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Based on the strong pressure of the returning airway in 31102 working face of Nalinhe No.2 well, the theoretical analysis and on-site measurement are used to study the mechanism of strong mine pressure in the fully mechanized mining face of the thick sandstone layer. The research shows that when the 31102 working face enters the double working face squares, the overburden structure of the stope is the standard load three zones structure; based on the double-sided stratum structure of 31102 working face, the mechanical model of the strike bearing pressure is established and the double is obtained. It is concluded that the working face of stage 31102 in double squares is 42.69 m to 215.67 m ahead to reach the stress condition of rock burst; the medium impact hazard range is 57.47 m to 137.02 m, the high stress transmitted by the range “DLZ” in the double squares phase 31102 along the empty roadway is the main reason for the strong occurrence of mine pressure. The strong control mechanism of the 31102 return airway is: to determine the reasonable pressure relief parameters, to release the coal volumetric energy through the large diameter borehole, and minimize the damage to the surrounding rock structure to ensure that the surrounding rock of the roadway is in “low stress-low disturbance” status.

The compound CuL(N3)(H2O) where L = 1,15-diaza-3,4:8,9-dibenzo-5,8-trioxacyclopentadecane-N-acetatic acid has been synthesized and characterized. X-ray crystallography reveals that copper(II) is five-coordinated in a square-pyramidal environment. The complex is a monoclinic system, a = 8.523(3) Å, b = 9.844(4) Å c = 25.895(9) Å, β = 97.69(3)°, V = 2153.4(3) Å3, Z = 4, λ(MoKα) = 0.71073, Rw = 0.0757 for 3081 observed reflections [I > 2σ(I)]. The copper atom is surrounded by two nitrogen atoms of the macrocycle, one oxygen atom from the carboxyl group in the ligand, one nitrogen atom from the azide group and one oxygen atom of coordinated water in a distorted square-pyramidal arrangement. The stability constant (5.8 × 106) of the complex, whose compostion is in the 1:1 metal:ligand molar ratio, was measured by electronic spectra in dimethyl formamide (DMF) as solvent. The EPR spectrum of the complex shows g⊥ = 2.0373 and g∂ = 2.250. The results comfirm that the structure of the complex is a distorted square pyramid. [ABSTRACT FROM AUTHOR]

Abstract: 99mTc-N4-guanine (99mTc-N4amG) was synthesized and evaluated in this study. Cellular uptake and cellular fraction studies were performed to evaluate the cell penetrating ability. Biodistribution and planar imaging were conducted in breast tumor-bearing rats. Up to 17%ID uptake was observed in cellular uptake study with 40% of 99mTc-N4amG was accumulated in the nucleus. Biodistribution and scintigraphic imaging studies showed increased tumor/muscle count density ratios as a function of time. Our results demonstrate the feasibility of using 99mTc-N4amG in tumor specific imaging. [Copyright &y& Elsevier]

Objective: This study was to develop a cGMP grade of [(18)F]fluoropropoxytryptophan ((18)F-FTP) to assess tryptophan transporters using an automated synthesizer., Methods: Tosylpropoxytryptophan (Ts-TP) was reacted with K(18)F/kryptofix complex. After column purification, solvent evaporation, and hydrolysis, the identity and purity of the product were validated by radio-TLC (1M-ammonium acetate : methanol = 4 : 1) and HPLC (C-18 column, methanol : water = 7 : 3) analyses. In vitro cellular uptake of (18)F-FTP and (18)F-FDG was performed in human prostate cancer cells. PET imaging studies were performed with (18)F-FTP and (18)F-FDG in prostate and small cell lung tumor-bearing mice (3.7 MBq/mouse, iv)., Results: Radio-TLC and HPLC analyses of (18)F-FTP showed that the Rf and Rt values were 0.9 and 9 min, respectively. Radiochemical purity was >99%. The radiochemical yield was 37.7% (EOS 90 min, decay corrected). Cellular uptake of (18)F-FTP and (18)F-FDG showed enhanced uptake as a function of incubation time. PET imaging studies showed that (18)F-FTP had less tumor uptake than (18)F-FDG in prostate cancer model. However, (18)F-FTP had more uptake than (18)F-FDG in small cell lung cancer model., Conclusion: (18)F-FTP could be synthesized with high radiochemical yield. Assessment of upregulated transporters activity by (18)F-FTP may provide potential applications in differential diagnosis and prediction of early treatment response.

(99m)Tc-N4-guanine ((99m)Tc-N4amG) was synthesized and evaluated in this study. Cellular uptake and cellular fraction studies were performed to evaluate the cell penetrating ability. Biodistribution and planar imaging were conducted in breast tumor-bearing rats. Up to 17%ID uptake was observed in cellular uptake study with 40% of (99m)Tc-N4amG was accumulated in the nucleus. Biodistribution and scintigraphic imaging studies showed increased tumor/muscle count density ratios as a function of time. Our results demonstrate the feasibility of using (99m)Tc-N4amG in tumor specific imaging., (Published by Elsevier Ltd.)

Objective: To study the morphology of Y chromosome and microdeletion of the correlated specific azoospermia factor(AZF) region on Y chromosome in cases of azoospermia and to identify the genetic diagnosis made for male infertility patients., Methods: Peripheral blood samples were taken from two patients with azoospermia, and then were examined by use of G banding, C banding cytogenetic analysis and multiplex polymerase chain reaction (PCR) microdeletion analysis., Results: The karyotypes of the two cases were 45, X, -Y, -22, +der(Y)t(Y;22)(q11.2;q11.2) and 46, XY, del(Y)(q11.2) respectively. In 12 sequence-tagged sites(STS) of AZFa, AZFb, AZFd, AZFc, only one was detected in the first case and two were detected in the other case., Conclusion: The cytogenetic analysis and the detection of AZF microdeletion on Y chromosome are essential to the final genetic diagnosis to be made for male infertility patients.