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Your search keyword '"Zhao, Junya"' showing total 18 results
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18 results on '"Zhao, Junya"'

6. Upregulating HIF‐1α to Boost the Survival of Neural Stem Cells via Functional Peptides‐Complexed MRI‐Visible Nanomedicine for Stroke Therapy

Author

Wang, Zhe, primary, Cai, Yujun, additional, Zhang, Qinyuan, additional, Li, Jianing, additional, Lin, Bingling, additional, Zhao, Junya, additional, Zhang, Fang, additional, Li, Yunhua, additional, Yang, Xieqing, additional, Lu, Liejing, additional, Shuai, Xintao, additional, and Shen, Jun, additional

Published
2022
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7. Upregulating microRNA‐210 to Inhibit Apoptosis of Neural Stem Cells with an MRI–Visible Nanomedicine for Stroke Therapy

Author

Zhang, Qinyuan, primary, Cai, Jiali, additional, Wang, Zhiyong, additional, Wang, Zhe, additional, Lin, Bingling, additional, Zhao, Junya, additional, Mao, Jiaji, additional, Li, Yunhua, additional, Li, Jianing, additional, Yang, Xieqing, additional, Shuai, Xintao, additional, Lu, Liejing, additional, and Shen, Jun, additional

Published
2022
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17. Generation of reporter hESCs by targeting EGFP at the CD144 locus to facilitate the endothelial differentiation.

Author

Hu, Zhiqing, Wu, Yong, Zhou, Miaojin, Wang, Xiaolin, Pang, Jialun, Li, Zhuo, Feng, Mai, Wang, Yanchi, Hu, Qian, Zhao, Junya, Liu, Xionghao, Wu, Lingqian, and Liang, Desheng

Subjects
EMBRYONIC stem cells, GREEN fluorescent protein, ENDOTHELIAL cells, CELL differentiation, REPORTER genes
Abstract

Reporter embryonic stem cell (ESC) lines with tissue-specific reporter genes may contribute to optimizing the differentiation conditions in vitro as well as trafficking transplanted cells in vivo. To optimize and monitor endothelial cell (EC) differentiation specifically, here we targeted the enhanced green fluorescent protein (EGFP) reporter gene at the junction of 5'UTR and exon2 of the endothelial specific marker gene CD144 using TALENs in human ESCs (H9) to generate a EGFP-CD144-reporter ESC line. The reporter cells expressed EGFP and CD144 increasingly and specifically without unexpected effects during the EC differentiation. The EC differentiation protocol was optimized and applied to EC differentiation from hiPSCs, resulting in an efficient and simplified endothelial differentiation approach. Here we created our own optimized and robust protocol for EC differentiation of hESCs and hiPSCs by generating the lineage- specific site- specific integration reporter cell lines, showing great potential to be applied in the fields such as trafficking gene and cell fate in vivo in preclinical animal models. [ABSTRACT FROM AUTHOR]

Published
2018
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18. Upregulating HIF‐1αto Boost the Survival of Neural Stem Cells via Functional Peptides‐Complexed MRI‐Visible Nanomedicine for Stroke Therapy

Author

Wang, Zhe, Cai, Yujun, Zhang, Qinyuan, Li, Jianing, Lin, Bingling, Zhao, Junya, Zhang, Fang, Li, Yunhua, Yang, Xieqing, Lu, Liejing, Shuai, Xintao, and Shen, Jun

Abstract

Neural stem cells (NSCs) transplantation has been considered as a promising strategy for the treatment of ischemic stroke. However, the therapeutic prospect is limited by the poor control over the survival, migration, and maturation of transplanted NSCs. Upregulating hypoxia inducible factor (HIF)‐1αexpression in stem cells can improve the survival and migration of NSCs grafted for stroke therapy. Functional peptide drugs, which could inhibit endogenous HIF‐1αubiquitination, might be used to effectively upregulate the HIF‐1αexpression in NSCs, thereby to improve the therapeutic effect in ischemia stroke. Herein, a magnetic resonance imaging (MRI)‐visible nanomedicine is developed to codeliver functional peptides and superparamagnetic iron oxide (SPIO) nanoparticles into NSCs. This nanomedicine not only promotes the survival and migration ability of NSCs but also allows an in vivo tracking of transplanted NSCs with MRI. The results demonstrate the great potential of the functional peptides‐complexed multifunctional nanomedicine in boosting the therapeutic effect of stem cell‐based therapy in stroke. Labeling neural stem cells (NSCs) with superparamagnetic iron oxide (SPIO)‐loaded, methoxy‐polyethylene glycol (mPEG)‐modified tetraethylenepentamine (TEPA) and cholic acid (CA)‐based micelles (PTC) can promote the survival and migration of NSCs via upregulation of hypoxia inducible factor‐1α(HIF‐1α). This magnetic resonance imaging (MRI)‐visible nanomedicine can enhance the therapeutic effect and allow in vivo MRI tracking of NSCs in a mouse model of acute stroke.

Published
2022
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