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Abstract: The transfer and distribution of 95Zr in a simulated broadbean-soil system was studied by using isotope-tracer techniques. The results showed that the 95Zr was mainly concentrated in the haulm, pod and root, and the activity concentration of 95Zr in these tissues reached the maximum in the initial stage then decreased continuously. The activity concentration of 95Zr in edible part-bean was relatively lower, which was just near to the detection limit. The 95Zr in soil was mainly (97%) deposited in surface layer soil (0–6 cm), indicating that the 95Zr absorbed by surface soil could not be moved downwards easily because of the strong adsorption. The dynamics of 95Zr concentrations in broadbean and soil were also confirmed by application of nonlinear regression method. [Copyright &y& Elsevier]

Objective: To explore the role of Nrf2/ARE pathway in skeletal muscle ischemia/reperfusion(I/R) injury preconditioning by dexmedetomidine(DEX)., Methods: Twenty-eight SD rats were randomly divided into sham-operated(Sham group)、I/R group、I/R+ DEX(DEX group) and I/R+DEX +Atipamezole (Atip group). In the Atip group, Atip(250 μ g/kg) and DEX(25 μ g/kg) were injected together after anesthesia; In the Sham and I/R groups, the homologous saline was also injected at the same time; In the DEX group, the homologous DEX and saline were coinjected. After 30 minutes, the hind limb ischemia was induced by clamping the common femoral artery and ligaturing collateral circulation. After 3 h of ischemia, the clamp and tourniquet were removed and the rats underwent 2 h of reperfusion. We measured plasma concentrations of lactate dehydrogenase (LDH) and creatine kinase(CK). The gastrocnemius muscle was harvested and immediately stored at -80℃ for the assessment of malondialdehyde(MDA)、superoxide dismutase(SOD) and Nrf2/HO-1 protein detected by Western blot. The other section muscle was stored in triformol for immunohistochemical and HE staining. The wet/dry was also immediately detecting., Results: The levels of wet/dry、MDA、LDH、CK、Nrf2 and HO-1 were higher( P <0.05) while the level of SOD was lower( P <0.05) in the I/R group than those in sham group. The levels of wet/dry、MDA、LDH、CK were significantly lower( P <0.05) yet the levels of SOD and Nrf2/HO-1 were significantly higher( P <0.05) in DEX group than those in I/R group. However, Atip reversed the effect of DEX in Atip group, each of indicators had significant changes compared with those in the DEX group( P <0.05)., Conclusions: Nrf2 protein was expressed in skeletal muscle of rat and DEX could promote its level in nucleus by α-adrenergic receptor. The down-stream products of Nrf2 have the effect of antioxidant.