The study was to explore the combination of fermentation strains and solid-state fermentation technology to improve the nutritional value of Maotai-flavor liquor grains and the application effect of distiller's grains on lambs. Using Maotai-flavor liquor lees as a single fermentation substrate, using eight combinations of six microbial strains for solid-state fermentation, using orthogonal experiment design to further optimize the conditions of the fermentation substrate moisture, fermentation temperature and fermentation time, to carry out solid-state fermentation scale-up test in production workshop, and the relevant nutritional indicators and mycotoxins were detected. 27 weaned female lambs with similar body weight and age were randomly divided into three groups, with three replicates in each group and three lambs in each replicate. The lambs in control group were fed with a basal diet, the lambs in group I were fed with a basal diet + 50 g/d MFFDG, and the lambs in group II were fed with a basal diet + 150 g/d MFFDG. The experiment lasted for 13 w. The results showed that the eight strains combinations could significantly increase the true protein content in distiller's grains (P<0.05). Group E (0.10% S. cerevisiae + 0.20% C. utilis + 0.0,5% B. subtilis+ 0.05% A. niger+ 0.05% A. oryzae), group G (0.10% S. cerevisiae + 0.05% A.niger+ 0.05% A. oryzae) and group F (0.10% S. cerevisiae + 0.25% C. utilis+ 0.05% B. subtilis + 0.05% B. licheniformis + 0.05% A. niger + 0.05% A. oryzae) had the better improvement effect. The optimum fermentation process was 38 1C with 55% water content for 5 d. The content of true protein, crude protein and calcium in Maotai-flavor distiller's grains were significantly increased (P<0.05), and the contents of crude fibre and organic matter were significantly decreased (P<0.05) compared with those before fermentation, and emetic toxin was significantly increased compared with before fermentation (P<0.05), but the content was far lower than the limit level of feed production. Feeding fermented lees to weaned lambs could significantly increase average daily gain (P<0.05) and decrease feed to gain ratio (P<0.05), but it had no significant effect on average daily feed intake (P>0.05). The experiment indicates that the combination of S. cerevisiae, A. niger and A. oryzae and the optimized conditions can significantly improve the nutritional value of white distiller's grains. Adding MFFDG in basal diet can effectively improve the performance of weaned lambs. [ABSTRACT FROM AUTHOR]