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Your search keyword '"Zhao, Zhen-Wang"' showing total 38 results
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38 results on '"Zhao, Zhen-Wang"'

9. A novel nude mouse model for studying the pathogenesis of endometriosis

Author

Li-Ping, Li, Zeng-Ming, Li, Zhao-Zhen, Wang, Yu-Fen, Cheng, De-Ming, He, Ge, Chen, Bian-Na, Cao, Yang, Zou, and Yong, Luo

Subjects
Cancer Research, Immunology and Microbiology (miscellaneous), General Medicine
Abstract

Endometriosis is a common female gynecological disease that is characterized by the presence of functional endometrial tissue outside the uterine cavity. At present, many animal models have been established. However, previous studies consistently use human endometrial tissue implanted in the subcutaneous or abdominal cavity for modeling and rarely use endometrial cells. In the present study, we ascertained whether immortalized stromal and/or epithelial endometrial cells are able to induce subcutaneous endometriosis in nude mice. Mixed human immortalized endometriosis stromal and epithelial cells, but not the cells of Group 1 or Group 2, were successfully constructed and led to endometriotic-like lesions. The endometriosis-like lesions observed in nude mice consisted of endometriosis-like glands lined with columnar epithelial cells and surrounded by stromal cells in the fibrous fatty connective tissue. Immunofluorescence analysis showed that glandular epithelial cells were intensely stained for E-cadherin and cytokeratin 7, and surrounding stromal cells were mildly stained for neprilysin (CD10) and vimentin. Moreover, the cells present in the endometriosis-like lesions were of human origin. Our data indicate that the mixture of human immortalized endometriosis stromal cells and epithelial cells is able to establish subcutaneous endometriosis lesions in nude mice. This model could be used to understand the molecular mechanisms involved in the occurrence and development of endometriosis.

Published
2022

12. Additional file 1 of Asprosin inhibits macrophage lipid accumulation and reduces atherosclerotic burden by up-regulating ABCA1 and ABCG1 expression via the p38/Elk-1 pathway

Author

Zou, Jin, Xu, Can, Zhao, Zhen-Wang, Yin, Shan-Hui, and Wang, Gang

Abstract

Additional File 1: Figure S1. Asprosin suppresses the production and secretion of IL-1β and IL-6. A, B THP-1 macrophage-derived foam cells were transfected with LV-Mock or LV-Asprosin for 72 h (n=3). A Measurement of IL-1β and IL-6 mRNA expression by qRT-PCR. B The cell culture supernatant was collected and then subjected to ELISA for detecting the levels of IL-1β and IL-6. Data are the mean ± SD. **P < 0.01.

Published
2022
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13. CORRIGENDUM: MicroRNA-182 Promotes Lipoprotein Lipase Expression and Atherogenesisby Targeting Histone Deacetylase 9 in Apolipoprotein E-Knockout Mice

Author

Cheng, Hai-Peng, primary, Gong, Duo, additional, Zhao, Zhen-Wang, additional, He, Ping-Ping, additional, Yu, Xiao-Hua, additional, Ye, Qiong, additional, Huang, Chong, additional, Zhang, Xin, additional, Chen, Ling-Yan, additional, Xie, Wei, additional, Zhang, Min, additional, Li, Liang, additional, Xia, Xiao-Dan, additional, Ouyang, Xin-Ping, additional, Tan, Yu-Lin, additional, Wang, Zong-bao, additional, Tian, Guo-Ping, additional, Zheng, Xi-Long, additional, Yin, Wei-Dong, additional, and Tang, Chao-Ke, additional

Published
2022
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18. Suppression of PRDX4 inhibits cell proliferation and invasion of ectopic endometrial stromal cells in endometriosis

Author

Yang Zou, Li-Ping Li, Liqun Wang, Ziyu Zhang, Yong Luo, Zhao-Zhen Wang, Fa-Ying Liu, and Ou-Ping Huang

Subjects
Gene knockdown, Stromal cell, medicine.diagnostic_test, Chemistry, Cell growth, Endocrinology, Diabetes and Metabolism, Endometriosis, Obstetrics and Gynecology, Transfection, Peroxiredoxins, Flow cytometry, Andrology, Blot, Endocrinology, Apoptosis, Case-Control Studies, medicine, Humans, Female, Viability assay, Molecular Targeted Therapy, RNA, Small Interfering, Stromal Cells, Reactive Oxygen Species, Cell Proliferation
Abstract

Oxidative stress (OS) has been proposed to play a role in the development of EMs. Peroxiredoxins are a family of antioxidant proteins that exhibit peroxidase activity in a thioredoxin-dependent manner, protecting cells against OS. The Western blotting results showed that the relative expression of PRDX4 was significantly increased in ectopic endometria compared with the normal endometria of EMs-free (p

Published
2020
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25. Pregnancy-Associated Plasma Protein-A Accelerates Atherosclerosis by Regulating Reverse Cholesterol Transport and Inflammation

Author

Tang, Shi-Lin, primary, Zhao, Zhen-Wang, additional, Liu, Shang-Ming, additional, Wang, Gang, additional, Yu, Xiao-Hua, additional, Zou, Jin, additional, Wang, Si-Qi, additional, Dai, Xiao-Yan, additional, Fu, Min-Gui, additional, Zheng, Xi-Long, additional, Zhang, Da-Wei, additional, Fu, Hui, additional, and Tang, Chao-Ke, additional

Published
2019
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26. MicroRNA-377 Inhibits Atherosclerosis by Regulating Triglyceride Metabolism Through the DNA Methyltransferase 1 in Apolipoprotein E-Knockout Mice

Author

Chen, Ling-Yan, primary, Xia, Xiao-Dan, additional, Zhao, Zhen-Wang, additional, Gong, Duo, additional, Ma, Xiao-Feng, additional, Yu, Xiao-Hua, additional, Zhang, Qiang, additional, Wang, Si-Qi, additional, Dai, Xiao-Yan, additional, Zheng, Xi-Long, additional, Zhang, Da-Wei, additional, Yin, Wei-Dong, additional, and Tang, Chao-Ke, additional

Published
2018
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27. Heat shock protein 70 accelerates atherosclerosis by downregulating the expression of ABCA1 and ABCG1 through the JNK/Elk-1 pathway

Author

Zhao, Zhen-Wang, primary, Zhang, Min, additional, Chen, Ling-Yan, additional, Gong, Duo, additional, Xia, Xiao-Dan, additional, Yu, Xiao-Hua, additional, Wang, Si-Qi, additional, Ou, Xiang, additional, Dai, Xiao-Yan, additional, Zheng, Xi-Long, additional, Zhang, Da-Wei, additional, and Tang, Chao-Ke, additional

Published
2018
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28. Research of Water Shortage Information Detection System for Crop Growth

Author

Hui Chen, Yue Liu, Meng Li, Zhao Zhen Wang, and Mei Xia Yang

Subjects
Canopy, Crop, Precision irrigation, Water conservation, Data acquisition, Deficit irrigation, Environmental science, General Medicine, Precision agriculture, Agricultural engineering, Water scarcity
Abstract

Precision irrigation is an important subsystem of precision agriculture, which is helpful to improve the water utilization of agricultural irrigation, so as to achieve the purpose of water conservation. For the issues of crop water shortage information timely and accurately, it designed and implemented a water shortage information detection system in the growth of crop based on canopy temperature and environmental factors. The system used the STM32 processor as the core, configured a variety of sensors, acquired and processed crop canopy temperature and various kinds of crops micro-environment information, obtained crop water shortage information through crop water stress index (CWSI), so as to guide the agricultural irrigation. The test proves that the system is working in good condition, can be effective for data acquisition and processing, so as to achieve the design requirements.

Published
2013

30. [Retraction]MicroRNA-134 Promotes the Development of Atherosclerosis Via the ANGPTL4/LPL Pathway in Apolipoprotein E Knockout Mice

Author

Ye, Qiong, primary, Tian, Guo-Ping, additional, Cheng, Hai-Peng, additional, Zhang, Xin, additional, Ou, Xiang, additional, Yu, Xiao-Hua, additional, Tan, Ru-Qi, additional, Yang, Feng-Yun, additional, Gong, Duo, additional, Huang, Chong, additional, Pan, Yan-Jun, additional, Zhang, Jie, additional, Chen, Ling-Yan, additional, Zhao, Zhen-Wang, additional, Xie, Wei, additional, Li, Liang, additional, Zhang, Min, additional, Xia, Xiao-Dan, additional, Zheng, Xi-Long, additional, and Tang, Chao-Ke, additional

Published
2018
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32. MicroRNA-182 Promotes Lipoprotein Lipase Expression and Atherogenesisby Targeting Histone Deacetylase 9 in Apolipoprotein E-Knockout Mice

Author

Cheng, Hai-Peng, primary, Gong, Duo, additional, Zhao, Zhen-Wang, additional, He, Ping-Ping, additional, Yu, Xiao-Hua, additional, Ye, Qiong, additional, Huang, Chong, additional, Zhang, Xin, additional, Chen, Ling-Yan, additional, Xie, Wei, additional, Zhang, Min, additional, Li, Liang, additional, Xia, Xiao-Dan, additional, Ouyang, Xin-Ping, additional, Tan, Yu-Lin, additional, Wang, Zong-bao, additional, Tian, Guo-Ping, additional, Zheng, Xi-Long, additional, Yin, Wei-Dong, additional, and Tang, Chao-Ke, additional

Published
2017
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34. MicroRNA-27 Prevents Atherosclerosis by Suppressing Lipoprotein Lipase-Induced Lipid Accumulation and Inflammatory Response in Apolipoprotein E Knockout Mice

Author

Xie, Wei, primary, Li, Liang, additional, Zhang, Min, additional, Cheng, Hai-Peng, additional, Gong, Duo, additional, Lv, Yun-Cheng, additional, Yao, Feng, additional, He, Ping-Ping, additional, Ouyang, Xin-Ping, additional, Lan, Gang, additional, Liu, Dan, additional, Zhao, Zhen-Wang, additional, Tan, Yu-Lin, additional, Zheng, Xi-Long, additional, Yin, Wei-Dong, additional, and Tang, Chao-Ke, additional

Published
2016
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35. Cystathionine γ-lyase(CSE)/hydrogen sulfide system is regulated by miR-216a and influences cholesterol efflux in macrophages via the PI3K/AKT/ABCA1 pathway

Author

Gong, Duo, primary, Cheng, Hai-peng, additional, Xie, Wei, additional, Zhang, Min, additional, Liu, Dan, additional, Lan, Gang, additional, Huang, Chong, additional, Zhao, Zhen-wang, additional, Chen, Ling-yan, additional, Yao, Feng, additional, Tan, Yu-lin, additional, Li, Liang, additional, Xia, Xiao-dan, additional, Zheng, Xi-long, additional, Wang, Zong-bao, additional, and Tang, Chao-ke, additional

Published
2016
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36. CXCL12 promotes atherosclerosis by downregulating ABCA1 expression via the CXCR4/GSK3β/β-catenin T120 /TCF21 pathway.

Author

Gao JH, He LH, Yu XH, Zhao ZW, Wang G, Zou J, Wen FJ, Zhou L, Wan XJ, Zhang DW, and Tang CK

Subjects
Animals, Apolipoproteins E deficiency, Atherosclerosis genetics, Atherosclerosis pathology, Cholesterol metabolism, Down-Regulation, HEK293 Cells, Humans, Macrophages metabolism, Male, Mice, Mice, Inbred C57BL, ATP Binding Cassette Transporter 1 genetics, Atherosclerosis metabolism, Basic Helix-Loop-Helix Transcription Factors metabolism, Chemokine CXCL12 metabolism, Glycogen Synthase Kinase 3 beta metabolism, Receptors, CXCR4 metabolism, beta Catenin metabolism
Abstract

CXC chemokine ligand 12 (CXCL12) is a member of the CXC chemokine family and mainly acts on cell chemotaxis. CXCL12 also elicits a proatherogenic role, but the molecular mechanisms have not been fully defined yet. We aimed to reveal if and how CXCL12 promoted atherosclerosis via regulating lipid metabolism. In vitro, our data showed that CXCL12 could reduce ABCA1 expression, and it mediated cholesterol efflux from THP-1-derived macrophages to apoA-I. Data from the luciferase reporter gene and chromatin immunoprecipitation assays revealed that transcription factor 21 (TCF21) stimulated the transcription of ABCA1 via binding to its promoter region, which was repressed by CXCL12. We found that CXCL12 increased the levels of phosphorylated glycogen synthase kinase 3β (GSK3β) and the phosphorylation of β-catenin at the Thr120 position. Inactivation of GSK3β or β-catenin increased the expression of TCF21 and ABCA1. Further, knockdown or inhibition of CXC chemokine receptor 4 (CXCR4) blocked the effects of CXCL12 on TCF21 and ABCA1 expression and the phosphorylation of GSK3β and β-catenin. In vivo, the overexpression of CXCL12 in Apoe -/- mice via lentivirus enlarged the atherosclerotic lesion area and increased macrophage infiltration in atherosclerotic plaques. We further found that the overexpression of CXCL12 reduced the efficiency of reverse cholesterol transport and plasma HDL-C levels, decreased ABCA1 expression in the aorta and mouse peritoneal macrophages (MPMs), and suppressed cholesterol efflux from MPMs to apoA-I in Apoe -/- mice. Collectively, these findings suggest that CXCL12 interacts with CXCR4 and then activates the GSK-3β/β-catenin T120 /TCF21 signaling pathway to inhibit ABCA1-dependent cholesterol efflux from macrophages and aggravate atherosclerosis. Targeting CXCL12 may be a novel and promising strategy for the prevention and treatment of atherosclerotic cardiovascular diseases., (Copyright © 2019 Gao et al.)

Published
2019
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37. MicroRNA-134 Promotes the Development of Atherosclerosis Via the ANGPTL4/LPL Pathway in Apolipoprotein E Knockout Mice.

Author

Ye Q, Tian GP, Cheng HP, Zhang X, Ou X, Yu XH, Tan RQ, Yang FY, Gong D, Huang C, Pan YJ, Zhang J, Chen LY, Zhao ZW, Xie W, Li L, Zhang M, Xia XD, Zheng XL, and Tang CK

Subjects
Animals, Atherosclerosis metabolism, Cholesterol metabolism, Cytokines metabolism, Foam Cells metabolism, Inflammation, Lipids chemistry, Lipoprotein Lipase metabolism, Macrophages metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, ApoE, Angiopoietin-Like Protein 4 blood, Angiopoietin-Like Protein 4 genetics, Atherosclerosis genetics, MicroRNAs blood, MicroRNAs genetics
Abstract

Aims: Atherosclerosis is the most common cause of cardiovascular disease, such as myocardial infarction and stroke. Previous study revealed that microRNA (miR)-134 promotes lipid accumulation and proinflammatory cytokine secretion through angiopoietin-like 4 (ANGPTL4)/lipid lipoprotein (LPL) signaling in THP-1 macrophages., Methods: ApoE KO male mice on a C57BL/6 background were fed a high-fat/high-cholesterol Western diet, from 8 to 16 weeks of age. Mice were divided into four groups, and received a tail vein injection of miR-134 agomir, miR-134 antagomir, or one of the corresponding controls, respectively, once every 2 weeks after starting the Western diet. After 8 weeks we measured aortic atherosclerosis, LPL Activity, mRNA and protein levels of ANGPTL4 and LPL, LPL/ low-density lipoprotein receptor related protein 1 Complex Formation, proinflammatory cytokine secretion and lipid levels., Results: Despite this finding, the influence of miR-134 on atherosclerosis in vivo remains to be determined. Using the well-characterized mouse atherosclerosis model of apolipoprotein E knockout, we found that systemic delivery of miR-134 agomir markedly enhanced the atherosclerotic lesion size, together with a significant increase in proinflammatory cytokine secretion and peritoneal macrophages lipid contents. Moreover, overexpression of miR-134 decreased ANGPTL4 expression but increased LPL expression and activity in both aortic tissues and peritoneal macrophages, which was accompanied by increased formation of LPL/low-density lipoprotein receptor-related protein 1 complexes in peritoneal macrophages. However, an opposite effect was observed in response to miR-134 antagomir., Conclusions: These findings suggest that miR-134 accelerates atherogenesis by promoting lipid accumulation and proinflammatory cytokine secretion via the ANGPTL4/LPL pathway. Therefore, targeting miR-134 may offer a promising strategy for the prevention and treatment of atherosclerotic cardiovascular disease.

Published
2018
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