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Advances in imaging technology have revolutionized the field of ophthalmology, changing the understanding, diagnosis, and treatment of ophthalmic diseases. Swept-source optical coherence tomography(SS-OCT)is a non-contact high-resolution imaging technology. It further improves imaging depth and scanning speed, adds new algorithms and features. The application of SS-OCT enables the three-dimensional evaluation of corneal structures, offering curvature and height maps for both the anterior and posterior surfaces of the cornea, as well as precise corneal thickness mapping. These invaluable tools aid ophthalmologists in effectively screening and diagnosing various corneal lesions such as keratoconus, corneal dystrophy, and degeneration. Moreover, the enhanced speed, accuracy, and sensitivity provided by SS-OCT measurements facilitate improved surgical planning and postoperative monitoring for patients undergoing refractive surgery or keratoplasty. This article reviews the development of SS-OCT technology and its potential clinical utility in corneal diseases and surgical application, in order to support more possible future research and clinical treatment.

To extend its shelf life, vacuum freeze-dried Toona sinensis (VFDTS) powder was packaged in sealed polyethylene (PE) bags, sealed aluminum foil bags, composite vacuum bags, or aluminum foil vacuum bags. Changes in the sensory quality, moisture content, color, flavor components and total bacterial count of VFDTS powder were monitored during storage under accelerated conditions at (55 ± 1) ℃ and (40 ± 3)% relative humidity (RH). The results showed that VFDTS powder packaged in sealed PE bags exhibited severe moisture absorption and browning with prolonged storage time, and the total bacterial count significantly increased. On the other hand, the moisture content, color and total bacterial count of the powder stored in aluminum foil vacuum bags remained substantially unchanged. A total of 56 volatile compounds were identified in VFDTS powder stored in the different packaging systems, including 13 alcohols, 11 aldehydes, 3 acids, 3 terpenes, 7 ketones, 9 esters, one ether, 2 sulfur compounds, 3 alkanes, and 4 heterocycles. Principal component analysis (PCA) of the volatile compounds showed clear separation among VFDTS powder samples stored in the 4 packaging systems. Based on variable importance in projection (VIP > 1) from orthogonal partial least squares discriminant analysis (OPLS-DA), a total of 14 differential volatile compounds were selected, and their clustering heatmaps could be used to effectively classify the samples in different packaging systems. The samples in aluminum foil vacuum bags had the best quality, followed by sealed aluminum foil bags and composite vacuum bags, while the greatest changes in moisture, color, flavor and microbiological quality were observed in VFDTS powder in sealed PE bags during storage.

Objective To analyze the effects of fat mass and obesity-associated (FTO) gene, IL-6, and HSP-60 gene polymorphism on the incidence rate and prognosis of breast cancer (BCa) for patients with type 2 diabetes mellitus (T2DM). Results A total of 1551 patients with BCa were included in the experimental group and 1605 women of the same age who participated in physical examination were included in the control group. The clinical data of the 3156 participants were collected through the baseline data questionnaire, and the genotypes of FTO, IL-6, and HSP-60 single-nucleotide polymorphism (SNP) were determined through blood sample detection. The predictive value of the three SNPs for the incidence risk of BCa for T2DM patients was evaluated. The OS of 1168 patients with BCa was obtained through follow-up, and the effects of the three SNPs and T2DM on OS of BCa patients were evaluated. Results The three loci were FTO rs3751812, IL-6 rs1800796, and HSP-60 rs2605039. The BCa incidence rate for T2DM women with wild homozygous SNP genotype was significantly higher than that for non-T2DM women (FTO: χ2=3.530, P=0.013; IL-6: χ2=6.288, P=0.029; HSP-60: χ2=4.926, P=0.005). The three wild homozygous genotypes were independent risk factors that influenced the incidence rate of BCa (all P < 0.05). Patients with HSP-60 rs2605039 (GT+TT) genotype had better OS (P=0.031). Conclusion FTO, IL-6, and HSP-60 gene polymorphisms have certain value in BCa prediction for T2DM patients. Patients with BCa and HSP-60 rs2605039 GT+TT genotype have high OS.

[Purpose/Significance] Soil microorganism is the most active component of soil, which is the key factor of a healthy soil ecosystem Therefore, to investigate the hotspots, frontiers and directions of soil microorganism research is helpful for researchers to understand research topics and directions. [Method/Process] Using CiteSpace V software papers of soil microbiology published by key journals from 2010 to 2019 collected by CNKI were used to analyze the distribution characteristics of authors, institutions and journals, and to draw the knowledge graph of keyword co-occurrence, burst terms distribution and the time line map of keyword burst terms. [Results/Conclusions] There are more than 400 articles about soil microorganism area published in China every year, and the research in this field continues to draw attention. Northwest A&F University and University of Chinese Academy of Sciences are the key research institutions, and Shen Qirong, Yang Dianlin and Mao Zhiquan are the core author groups, which have made outstanding contributions to the development of this field. The research hotspots have evolved over time, and can be divided into four modules: First, indirect indicators of soil microorganisms (soil enzyme activity, soil uptake, soil microbial biomass carbon and nitrogen); second, mature development of research methods (Phospholipid-derived Fatty Acids, denaturing gradient Gel electrophoresis, Biolog-ECO technique, and high throughput sequencing); third, the visual representation of soil microorganisms (microbial community structure and functional diversity); Lastly, the impact of factors (soil type and land use, fertilization, crops, nitrogen deposition, and bio-char). The application of molecular biological methods such as high throughput sequencing and system biological methods, the development and utilization of bio-char, the influence of nitrogen deposition and the improvement measures should be paid more attention as the focus of future research.

Objective To determine the effects of maternal and infant separation(MS) on anxiety and depression-like behaviors, Wnt signaling molecules and receptors in the hippocampus of offspring rats. Methods Sixteen Sprague-Dawley (SD) pregnant rats (240~260 g) were randomly and equally divided into 2 groups. The offspring rats from the control group (CON) received no postpartum intervention, while those of the experiment group (MS) were given maternal-infant separation 3 h per day for 14 consecutive days since delivery. Elevated plus maze test (EPMT) and forced swimming test (FST) were conducted to evaluate the anxiety and depression-like behaviors of 1-month-old offspring rats. Real-time quantitative PCR was used to detect the levels of Wnt molecules in the hippocampus of the offspring rats, and Western blotting was adopted to determine the expression of Wnt2 and Wnt3a proteins as well as the Wnt signaling pathway inhibitory molecules DKK1 and sFRP3 in the hippocampus. Results The total number of arm entries (including open-arm and close-arm entries) was not statistically different between the MS and CON groups. However, the MS group had significantly lower percentages of open-arm entries (P < 0.01) and open-arm time (P < 0.05), and longer stationary time (P < 0.05) in the FST than the CON group, indicating obvious anxiety and depression-like behaviors. Moreover, the mRNA levels of Wnt2 and Wnt3a in the hippocampus were notably decreased (P < 0.01), while the protein levels of DKK1 and sFRP3 were increased remarkably (P < 0.01) in the MS group than the CON group. Conclusion Maternal and infant separation leads to depression and anxiety-like behaviors in offspring rats, which may be associated to the disorder of Wnt signaling molecules in the hippocampus.

Among the fascinating phenomena observed in two-dimensional (2D) magnets, the magneto-exciton effect stands out as a pivotal link between optics and magnetism. Although the excitonic effect has been revealed and exhibits a considerable correlation with the spin structures in certain 2D magnets, the underlying mechanism of the magneto-exciton effect remains underexplored, especially under high magnetic fields. Here we perform a systematic investigation of the spin-exciton coupling in 2D antiferromagnetic NiPS3 under high magnetic fields. When an in-plane magnetic field is applied, the exceptional sharp excitonic emission at ~1.4756 eV exhibits a Zeeman-like splitting with g ≈ 2.0, experimentally identifying the exciton as an excitation of dominant triplet-singlet character. By examining the polarization of excitonic emission and simulating the spin evolution, we further verify the correlation between excitonic emission and Néel vector in NiPS3. Our work elucidates the mechanism behind the spin-exciton coupling in NiPS3 and establishes a strategy for optically probing the spin evolutions in 2D magnets.

With the changing behavior of land use by explosive population growth, the ecological environment is constantly disturbed and destroyed by humans, and the value of ecosystem services is obviously degraded. After the introduction of the national new-type urbanization plan, counties have become the key areas to promote new-type urbanization, and county land use is exceptionally active. Therefore, it is especially urgent to grasp the impact of county land use changes on ecosystem service values. Based on remote sensing satellite image interpretation to obtain county land use classification data, the changes of ecosystem service values in County between 1995 and 2015 were thoroughly explored. The research results show that the total value of ecosystem services has increased in most regions during the 20 years, among which, the service value of Shunchang County has increased most significantly. In terms of the service values of different land use types in each region, woodland is the main contributor to ecological services in different regions. From different time periods, Jian’ou City had the highest value of woodland ecosystem services in 1995 and 2015. In contrast, Songxi County had a lower value of services for each ecosystem type during 20 years.

UNLABELLED: Patients with H3K27M-mutant diffuse midline glioma (DMG) have no proven effective therapies. ONC201 has recently demonstrated efficacy in these patients, but the mechanism behind this finding remains unknown. We assessed clinical outcomes, tumor sequencing, and tissue/cerebrospinal fluid (CSF) correlate samples from patients treated in two completed multisite clinical studies. Patients treated with ONC201 following initial radiation but prior to recurrence demonstrated a median overall survival of 21.7 months, whereas those treated after recurrence had a median overall survival of 9.3 months. Radiographic response was associated with increased expression of key tricarboxylic acid cycle-related genes in baseline tumor sequencing. ONC201 treatment increased 2-hydroxyglutarate levels in cultured H3K27M-DMG cells and patient CSF samples. This corresponded with increases in repressive H3K27me3 in vitro and in human tumors accompanied by epigenetic downregulation of cell cycle regulation and neuroglial differentiation genes. Overall, ONC201 demonstrates efficacy in H3K27M-DMG by disrupting integrated metabolic and epigenetic pathways and reversing pathognomonic H3K27me3 reduction. SIGNIFICANCE: The clinical, radiographic, and molecular analyses included in this study demonstrate the efficacy of ONC201 in H3K27M-mutant DMG and support ONC201 as the first monotherapy to improve outcomes in H3K27M-mutant DMG beyond radiation. Mechanistically, ONC201 disrupts integrated metabolic and epigenetic pathways and reverses pathognomonic H3K27me3 reduction. This article is featured in Selected Articles from This Issue, p. 2293.

Wireless communication technology has been developed rapidly after entering the 21st century. Data transfer rate increased significantly as well as the bandwidth became wider and wider from 2G to 4G in wireless communication systems. Channel estimation is an import part of any communication systems; its accuracy determines the quality of the whole communication. Channel estimation methods of typical wireless communication systems such as UWB, 2G and 3G have been researched.