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16 results on '"Zheng, Yuanru"'

9. Zebrafish facilitates drug screening: potential of 3-deoxy-andrographoside from Chuanxinlian (Herba Andrographitis Paniculatae) as an anti-inflammatory agent

Author

HE, Xuemei, XIAO, Junjie, FAN, Chunlin, LU, Zibin, CAO, Huihui, YU, Linzhong, ZHENG, Yuanru, and LIU, Junshan

Subjects
Inflammation, Lipopolysaccharides, Lactones, Plant Extracts, Anti-Inflammatory Agents, Drug Evaluation, Preclinical, NF-kappa B, Animals, Diterpenes, Research Articles, Zebrafish, Andrographis paniculata
Abstract

OBJECTIVE: To systematically evaluate the in vivo anti-inflammatory potential of diterpene lactones from Chuanxinlian (Herba Andrographitis Paniculatae) (AP). METHODS: We firstly adopted zebrafish, a novel and ideal animal model for high-throughput drug screening, to investigate the in vivo anti-inflammatory activities of 17 diterpene lactones isolated from AP. RESULTS: The results showed that most of diterpene lactones displayed significant anti-inflammatory effects in lipopolysaccharide microinjection-, copper sulfate exposure- or tail transection-induced zebrafish inflammation models. Moreover, diterpene lactone 3-deoxy-andrographoside (AP-5) was firstly found to attenuate inflammatory responses, which was closely associated with the myeloid differentiation primary response 88/nuclear factor-kappa B and signal transducer and activator of transcription 3 pathways. CONCLUSION: Our research sheds light on the inestimable roles of zebrafish in high-throughput drug screening, elucidates the potent inhibitory effects of diterpene lactones against inflammation and indicates that AP-5 may serve as a potential alternative agent for the treatment of inflammatory diseases.

Published
2022

12. Total triterpenoids from apple peels exert pronounced anti‐breast‐cancer activity in vivo and in vitro.

Author

Yan, Wanyu, Chen, Cong, Zheng, Yuanru, Xu, Jingwen, Wang, Yihai, and He, Xiangjiu

Subjects
*TRITERPENOIDS, *CHINESE medicine, *INHIBITION of cellular proliferation, *CELLULAR signal transduction, *CELL proliferation, *FRUIT skins
Abstract

BACKGROUND RESULTS CONCLUSION Apples are among the most nutritionally valuable fruits and have a history of use in traditional Chinese medicine. Triterpenoids, the primary bioactive compounds found in apples, demonstrate significant antitumor activity.Following enrichment and optimization, the total content of major triterpenoids in total triterpenoids from apple peels (ATT) reached 5.76 g kg−1. The growth of MDA‐MB‐231 xenograft tumors was significantly inhibited after treatment with ATT. Network pharmacology analysis conclusively identified a close association between the antitumor effect of ATT and the phosphatidylinositol 3‐kinase–protein kinase B (PI3K‐Akt) signaling pathway. Experimental validation using MDA‐MB‐231 cells and a xenograft nude mouse model confirmed that ATT suppressed tumor cell proliferation effectively by modulating the PI3K‐Akt signaling pathway, which was consistent with the findings from network pharmacology. The total triterpenoids from apple peels also induced cell apoptosis by mediating the PI3K‐Akt signaling pathway.The total triterpenoids from apple peels can inhibit tumor cell proliferation and induce cell apoptosis effectively through the PI3K‐Akt signaling pathway, suggesting that ATT holds promise as a prospective therapeutic agent for breast cancer treatment. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Screening of Bufadienolides from Toad Venom Identifies Gammabufotalin as a Potential Anti-inflammatory Agent.

Author

Zheng, Yuanru, Deng, Lijuan, Cao, Huihui, Xu, Nishan, Zhang, Dongmei, Tian, Haiyan, Li, Baojing, Lu, Zibin, Ye, Wencai, Yu, Linzhong, Fan, Chunlin, and Liu, Junshan

Subjects
*STEROIDS analysis, *INFLAMMATION prevention, *VENOM, *LIPOPOLYSACCHARIDES, *ANTI-inflammatory agents, *ANIMAL experimentation, *CELLULAR signal transduction, *FISHES, *DNA-binding proteins, *ANURA, *MOLECULAR structure, *TRANSCRIPTION factors, *PHARMACODYNAMICS
Abstract

Toad venom (Chansu) is used in the treatment of infectious and inflammatory diseases in China and East/Southeast Asian countries. However, the anti-inflammatory components of toad venom have not yet been systematically evaluated and clearly defined. To investigate the anti-inflammatory effects of toad venom and identify new anti-inflammatory ingredients, we used zebrafish, an alternative drug screening model, to evaluate the anti-inflammatory effects of 14 bufadienolides previously isolated from toad venom. Most of the bufadienolides were found to exert significant anti-inflammatory effects on lipopolysaccharide-, CuSO4-, or tail transection-induced zebrafish inflammatory models. Moreover, gammabufotalin (6) inhibits lipopolysaccharide-induced inflammation by suppressing the myeloid differentiation primary response 88/nuclear factor-kappa B and STAT3 signal pathways. This study confirms the potential of zebrafish in drug screening, clarifies the anti-inflammatory effects of bufadienolides from toad venom, and indicates that gammabufotalin may be developed as a novel therapeutic agent for inflammatory diseases in the future. [ABSTRACT FROM AUTHOR]

Published
2022
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