15 results on '"Zheng-Qiu, Zhu"'
Search Results
2. Controlling Nutritional Status (CONUT) score is a prognostic marker in III-IV NSCLC patients receiving first-line chemotherapy
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Yi Zhang, Fei-Fei Kong, Zheng-Qiu Zhu, and Hai-Xia Shan
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Non‑small-cell lung cancer ,Prognostic factor ,CONUT score ,PNI ,SII ,Chemotherapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background To investigate the prognostic impact of the controlling nutritional status (CONUT) score in non-small-cell lung cancer (NSCLC) patients receiving first-line chemotherapy. Methods We retrospectively reviewed 278 consecutive patients undergoing chemotherapy for stage III-IV NSCLC between May 2012 and July 2020. CONUT score was calculated by incorporating serum albumin, total cholesterol, and total lymphocyte count. The patients were divided into two groups: CONUT ≥ 3 and CONUT
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- 2023
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3. Computed Tomography Features of Adnexal Torsion: A Meta-Analysis
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Ling-Shan, Chen, Jing, Li, Zheng-Qiu, Zhu, Pin, Wang, Zhi-Tao, Wang, Fu-Ting, Tang, Xu-Yu, Hu, and Zhong-Qiu, Wang
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- 2022
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4. Magnetic resonance imaging features for differentiating tuberculous from pyogenic spondylitis: a meta-analysis.
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Ling-Shan, Chen, Zheng-Qiu, Zhu, Jing, Li, Rui, Zhao, Li-Fang, Ling, Zhi-Tao, Wang, and Zhong-Qiu, Wang
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MAGNETIC resonance imaging , *SPONDYLITIS - Abstract
Objective: To perform a meta-analysis comparing the MRI features of tuberculous and pyogenic spondylitis, using histopathological results and/or blood culture as the standard reference. Materials and methods: PubMed, Embase, Web of Science, and Cochrane Library were searched for English-language studies on the MRI features of tuberculous and pyogenic spondylitis published between January 2010 and February 2023. Risk for bias and concerns regarding applicability were assessed using the Quality Assessment of Diagnostic Accuracy Studies-2 tool. Pooled MRI features' proportions were calculated using a bivariate random-effects model. Results: Thirty-two studies met the inclusion criteria: 21 for tuberculous spondylitis, three for pyogenic spondylitis, and eight for both. Of the nine informative MRI features comparing tuberculous spondylitis to pyogenic spondylitis, involvement of ≥ 2 vertebral bodies (92% vs. 88%, P =.004), epidural extension (77% vs. 25%, P <.001), paravertebral collection (91% vs. 84%, P <.001), subligamentous spread (93% vs. 24%, P <.001), thin and regular abscess wall (94% vs. 18%, P <.001), vertebral collapse (68% vs. 24%, P <.001), and kyphosis (39% vs. 3%, P <.01) were more suggestive of tuberculous spondylitis, while disc signal change (82% vs. 95%, P <.001) and disc height loss (22% vs. 59%, P <.001) were more suggestive of pyogenic spondylitis. Conclusion: Involvement of ≥ 2 vertebral vertebral bodies, soft tissue attribution, thin and regular abscess wall, vertebral collapse, and kyphosis were MRI features more common in tuberculous spondylitis, while disc signal change and height loss were more common in pyogenic spondylitis. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Carotid stiffening predicts cardiovascular risk stratification in mid-life: non-invasive quantification with ultrafast ultrasound imaging
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Hui Gao, Xue-Hui Ma, Wenjun Liu, Yun Luan, Chong Zou, Yi-Yun Wu, Hui Huang, Yin-Ping Wang, Bixiao Shen, Zheng-Qiu Zhu, Ling-Shan Chen, Shanshan He, and Xinyi Zhang
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medicine.medical_specialty ,business.industry ,Non invasive ,medicine.disease ,Stiffening ,Internal medicine ,Risk stratification ,medicine ,Cardiology ,Ultrasound imaging ,Arterial stiffness ,Radiology, Nuclear Medicine and imaging ,business ,Ultrashort pulse ,Pulse wave velocity - Abstract
Purpose: The present study investigated the association between Systematic COronary Risk Evaluation (SCORE)-estimated cardiovascular risk and carotid stiffening in a middle-aged population using ultrafast pulse wave velocity (ufPWV).Methods: This study enrolled 683 participants without known cardiovascular disease or diabetes mellitus who underwent ufPWV measurements. Clinical interviews, physical examinations, laboratory findings, carotid intima-media thickness (cIMT), pulse wave velocity (PWV) at the beginning of systole (PWV-BS), and PWV at the end of systole (PWV-ES) were assessed. Each participant underwent an assessment of SCORE risk based on major cardiovascular risk factors (CVRFs), including age, sex, smoking, systolic blood pressure (SBP), and total cholesterol (TC). Crude and adjusted odds ratios (ORs) with 95% confidence intervals and ordinal logistic regression were used. Overall CVRFs were adjusted to assess ORs.Results: cIMT and carotid stiffening in PWV-BS and PWV-ES were significantly different between sex subgroups (all P0.05).Conclusion: Carotid stiffening quantified by ufPWV is linked to SCORE categories, and elevated PWV-ES may aid in cardiovascular risk stratification.
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- 2022
6. Controlling Nutritional Status (CONUT) Score is a Prognostic Marker in III-IV NSCLC Patients Receiving First-line Chemotherapy
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Yi Zhang, Fei-Fei Kong, and Zheng Qiu Zhu
- Abstract
Background: To investigate the prognostic impact of the controlling nutritional status (CONUT) score in non-small-cell lung cancer (NSCLC) patients receiving first-line chemotherapy.Methods: We retrospectively reviewed 278 consecutive patients undergoing chemotherapy for stage III-IV NSCLC between May 2012 and July 2020. CONUT score was calculated by incorporating serum albumin, total cholesterol, and total lymphocyte count. The clinicopathological features and follow-up data were evaluated to compare the CONUT score with other prognostic indices, such as the systemic immune-inflammation index (SII) and prognostic nutritional index (PNI), in patients with NSCLC. Results: Applying cut-offs of ≥3 (CONUT), ≥443.607 (SII), and ≥49.05 (PNI). The high CONUT group had a significantly shorter progression-free survival and overall survival than the low CONUT group. A high CONUT score was significantly associated with older age, worse ECOG PS, advanced clinical stage, and lower PNI (all P < 0.05). In the univariate analysis, higher SII, higher CONUT, advanced clinical stage and lower PNI were associated with worse PFS (PConclusion: CONUT score is an independent prognostic indicator of poor outcomes for patients with stage III-IV NSCLC and is superior to the SII and PNI in terms of prognostic ability.Trial registration: retrospectively registered.
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- 2020
7. Absent atherosclerotic risk factors are associated with carotid stiffening quantified with ultrafast ultrasound imaging
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Hui Gao, Ling-Shan Chen, Ping Dai, Yi-Yun Wu, Yun Luan, Zheng-Qiu Zhu, Hui-Juan Sun, Fu-Ming Liu, Yin-Ping Wang, Xue-Hui Ma, Lin-Lin Wu, Chong Zou, Hui Huang, Xue-Zhong Jiang, and Fei Zou
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Adult ,Male ,medicine.medical_specialty ,Population ,Pulse Wave Analysis ,Carotid Intima-Media Thickness ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,education ,Pulse wave velocity ,Ultrasonography ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Infant ,Interventional radiology ,General Medicine ,Odds ratio ,medicine.disease ,Atherosclerosis ,Confidence interval ,Blood pressure ,030220 oncology & carcinogenesis ,cardiovascular system ,Cardiology ,Arterial stiffness ,Radiology ,business ,Body mass index - Abstract
To evaluate carotid stiffening in participants without conventional cardiovascular risk factors (CVRFs) by using ultrafast pulse wave velocity (ufPWV). The present study enrolled 517 participants without conventional CVRFs (CVRF-Free total population). Subjects in this population were defined as current non-smokers with untreated blood pressure
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- 2020
8. Carotid stiffness and atherosclerotic risk: non-invasive quantification with ultrafast ultrasound pulse wave velocity
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Niu Liu, Yun Luan, Fu-Ming Liu, Hui Huang, Ling-Shan Chen, Pin Wang, Lin-Lin Wu, Han Wang, and Zheng-Qiu Zhu
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Adult ,Carotid Artery Diseases ,Male ,medicine.medical_specialty ,Adolescent ,Systole ,Pulse Wave Analysis ,Carotid Intima-Media Thickness ,030218 nuclear medicine & medical imaging ,Young Adult ,03 medical and health sciences ,Vascular Stiffness ,0302 clinical medicine ,Carotid stiffness ,Risk Factors ,Internal medicine ,medicine ,Humans ,Pulse wave ,Radiology, Nuclear Medicine and imaging ,Prospective Studies ,cardiovascular diseases ,Pulse wave velocity ,Aged ,Ultrasonography ,Neuroradiology ,Aged, 80 and over ,medicine.diagnostic_test ,business.industry ,Ultrasound ,Age Factors ,Interventional radiology ,General Medicine ,Middle Aged ,Atherosclerosis ,medicine.disease ,Carotid Arteries ,Early Diagnosis ,030220 oncology & carcinogenesis ,cardiovascular system ,Arterial stiffness ,Cardiology ,Female ,Radiology ,business ,circulatory and respiratory physiology - Abstract
To evaluate the value of ultrafast pulse wave velocity (ufPWV) for the quantitative assessment of carotid stiffness and its associated with atherosclerosis (AS) risk. The present study included 233 patients with hyperlipoidaemia (AS risk group) and 114 healthy adults as the control group. The carotid (n = 694) intima-media thickness (cIMT), pulse wave velocity-beginning of systole (PWV-BS) and pulse wave velocity-end of systole (PWV-ES) were measured on sample images. Differences, distributive characteristics and correlation evaluation were assessed in patients (ages 18–29, 30–39, 40–49, 50–59, 60–69 and ≥70) and carotids (control group vs AS risk group). The cIMT, PWV-BS and PWV-ES increased with age; PWV-ES and cIMT showed an early significant increase in the 30–39 years group, whereas PWV-BS displayed a significant increase at 40–49 years compared with the 18- to 29-years group. Besides, PWV-ES correlated well with age compared with PWV-BS and cIMT. For carotid level, cIMT, PWV-BS and PWV-ES measurements were higher in the AS risk group compared with control. To compare the value of ufPWV and cIMT in early AS assessment, we subdivided groups into cIMT subgroups using a cut-off thickness of 0.050 cm. PWV-ES measurements were higher in the AS risk group compared with the control in the 0.040–0.050 cm (not thickened) and 0.051–0.060 cm (thickened) cIMT subgroups. Carotid ufPWV measurement at PWV-ES is a novel modality for the early diagnosis and quantitative assessment of arterial stiffness associated with atherosclerotic risk. • ufPWV technique is real-time and well repeatable for assessing carotid stiffness • ufPWV measurements increase and correlate well with age • PWV-ES is a quantitative predictor for the early assessment of AS
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- 2018
9. Chemical shift magnetic resonance imaging for distinguishing minimal-fat renal angiomyolipoma from renal cell carcinoma: a meta-analysis
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Jing Li, Zhongqiu Wang, Zheng-Qiu Zhu, Zhi-Tao Wang, Ling-Shan Chen, Ji-yang Jin, and Li-Feng Liang
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medicine.medical_specialty ,Angiomyolipoma ,Urology ,Chromophobe cell ,Kidney ,urologic and male genital diseases ,030218 nuclear medicine & medical imaging ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Renal cell carcinoma ,Statistical significance ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Carcinoma, Renal Cell ,neoplasms ,Neuroradiology ,medicine.diagnostic_test ,business.industry ,Magnetic resonance imaging ,General Medicine ,Publication bias ,medicine.disease ,Magnetic Resonance Imaging ,Kidney Neoplasms ,female genital diseases and pregnancy complications ,Adipose Tissue ,030220 oncology & carcinogenesis ,Meta-analysis ,Radiology ,business ,Clear cell - Abstract
To determine the performance of chemical shift signal intensity index (CS-SII) values for distinguishing minimal-fat renal angiomyolipoma (mfAML) from renal cell carcinoma (RCC) and to assess RCC subtype characterisation. We identified eligible studies on CS magnetic resonance imaging (CS-MRI) of focal renal lesions via PubMed, Embase, and the Cochrane Library. CS-SII values were extracted by lesion type and evaluated using linear mixed model-based meta-regression. RCC subtypes were analysed. Two-sided p value
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- 2017
10. Evidence of motor injury due to damaged corticospinal tract following acute hemorrhage in the basal ganglia region
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Si Yuan Hou, Ling Shan Chen, Zhong Qiu Wang, Xue Hu Wei, Zheng Qiu Zhu, Xiao Kun Fang, Yong Kang Liu, and Jing Li
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Adult ,Male ,Internal capsule ,Pyramidal Tracts ,lcsh:Medicine ,03 medical and health sciences ,0302 clinical medicine ,Corona radiata ,Basal ganglia ,Fractional anisotropy ,medicine ,Humans ,030212 general & internal medicine ,lcsh:Science ,Paresis ,Aged ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Basal Ganglia Hemorrhage ,Magnetic resonance imaging ,Anatomy ,Recovery of Function ,Middle Aged ,Magnetic Resonance Imaging ,Diffusion Tensor Imaging ,Motor Skills ,Corticospinal tract ,lcsh:Q ,Female ,medicine.symptom ,business ,human activities ,030217 neurology & neurosurgery ,Diffusion MRI - Abstract
The integrity of the corticospinal tract (CST) is significantly affected following basal ganglia haemorrhage. We aimed to assess the local features of CST and to effectively predict motor function by diffusion characteristics of CST in patients with motor injury following acute haemorrhage in the acute basal ganglia region. We recruited 37 patients with paresis of the lateral limbs caused by acute basal ganglia haemorrhage. Based on the automated fiber quantification method to track CST, assessed the character of each CST segment between the affected and contralateral sides, and correlated these with the Fugl–Meyer (FM) and Barthel Index (BI) scores at 6 months after onset. The fractional anisotropy (FA) values of the injured side of CST showed a significantly lower FA than the contralateral side along the tract profiles (p
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- 2019
11. Hysterosalpingo-contrast-sonography vs. magnetic resonance-hysterosalpingography for diagnosing fallopian tubal patency: A systematic review and meta-analysis
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Ling-Shan Chen, Xu-Yu Hu, Mei-Mei Zhang, Zheng-Qiu Zhu, Ye Qiang, Jing Li, Zhi-Tao Wang, and Zhongqiu Wang
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Adult ,Cochrane Library ,Multimodal Imaging ,Sensitivity and Specificity ,Fallopian Tube Patency Tests ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Hysterosalpingography ,Laparoscopy ,Fallopian Tubes ,Ultrasonography ,medicine.diagnostic_test ,Receiver operating characteristic ,business.industry ,Ultrasound ,Reproducibility of Results ,General Medicine ,Magnetic Resonance Imaging ,Confidence interval ,ROC Curve ,030220 oncology & carcinogenesis ,Meta-analysis ,Diagnostic odds ratio ,Female ,business ,Nuclear medicine - Abstract
Purpose To compare hysterosalpingo-contrast-sonography (HyCoSy) and magnetic resonance-hysterosalpingography (MR-HSG) in the diagnosis of fallopian tubal patency. Materials and methods The databases of PubMed, Embase, and the Cochrane Library were searched for records up to November 30, 2019. Studies involved in the diagnostic detection of HyCoSy or MR-HSG for fallopian tubal patency using conventional HSG or laparoscopy as the reference test were included. Data was analyzed by meta-analysis. We compared sensitivity, specificity, positive and negative likelihood ratios (PLR and NLR), diagnostic odds ratio (DOR), and summary receiver operating characteristic (sROC) plots of both HyCoSy and MR-HSG. Quality was assessed using the QUADAS-2 tool. Results The analysis included 24 articles involving 1340 patients. HyCoSy was studied in 17 studies, and MR-HSG was studied in seven studies. For HyCoSy in diagnosis of fallopian tubal patency, pooled sensitivity was 89 % (95 % confidence interval [CI], 87 %–91 %), and specificity was 93 % (95 % CI, 91 %–94 %). For MR-HSG in diagnosis of fallopian tubal patency, pooled sensitivity was 100 % (95 % CI, 98 %–100 %), and specificity was 82 % (95 % CI, 74 %–89 %). The sROC showed similar diagnostic accuracy for MR-HSG and HyCoSy. 3D/4D HyCoSy with ultrasound microbubbles had equal sensitivity (95 % vs. 100 %, P = 0.186) and significantly higher specificity (94 % vs. 82 %, P = 0.005) compared with MR-HSG. Conclusions HyCoSy and MR-HSG showed similar overall diagnostic performance for diagnosing fallopian tubal patency. 3D/4D HyCoSy with ultrasound microbubbles could significantly improve the diagnostic specificity of HyCoSy.
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- 2020
12. Continuous infusion of a large dose of CF (folinic acid) and 5-FU combined with CDDP in the treatment of advanced esophageal cancer
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Hong-Xing Cai, Zheng-Qiu Zhu, and Zu-An Zhu
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0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Time Factors ,Esophageal Neoplasms ,Nausea ,medicine.medical_treatment ,Leucovorin ,Kaplan-Meier Estimate ,Gastroenterology ,Drug Administration Schedule ,Metastasis ,03 medical and health sciences ,Folinic acid ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,Carcinoma ,medicine ,Humans ,Pharmacology (medical) ,Karnofsky Performance Status ,Infusions, Intravenous ,Aged ,Neoplasm Staging ,Proportional Hazards Models ,Pharmacology ,Chemotherapy ,Chi-Square Distribution ,business.industry ,Therapeutic effect ,Middle Aged ,medicine.disease ,Regimen ,030104 developmental biology ,Treatment Outcome ,030220 oncology & carcinogenesis ,Lymphatic Metastasis ,Vomiting ,Carcinoma, Squamous Cell ,Female ,Esophageal Squamous Cell Carcinoma ,Fluorouracil ,medicine.symptom ,Cisplatin ,business ,medicine.drug - Abstract
5-fluorouracil (5-FU) and cisplatin (CDDP) are common chemotherapy drugs used in the treatment of patients with advanced esophageal cancer. We investigated the efficacy of adding a continuous infusion of a large dose of a common adjuvant, citrovorumfactor (CF), to the traditional 5-FU/wCDDP regimen. 50 patients with advanced esophageal cancer were treated with a continuous infusion of CF, 5-FU, and CDDP, and the short-term effects, adverse reactions, and survival periods after treatment were analyzed. Overall, the treatment was effective in 58% of patients, and the therapeutic effects of the first-line of chemotherapy were significantly better than the second-line (u = 4.121, p < 0.05). Patients experienced severe nausea and vomiting in 18.7% of the treatment cycles and experienced severe hair loss or leucopenia in 1.9% of the treatment cycles. The majority of the treatment cycles produced only mild side effects. The median survival period following chemotherapy treatment was 10.6 months (95% confidence interval was 8.146 ~ 13.054 months), with the median survival time of patients with a Karnofsky Performance Status (KPS) score ≥ 80 being significantly longer than that of patients with KPS scores < 80 (χ2 = 41.595, p < 0.05). The median survival time of patients with metastasis to the lymph nodes and surrounding tissue was significantly longer than that of patients with visceral metastasis (χ2 = 32.246, p < 0.05). Cox regression analysis showed that KPS scores before the treatment < 80 (relative risk (RR= = 1.635) and the incidence of visceral metastasis (RR = 1.875) were associated with survival time (p < 0.05). These results suggest that the continuous infusion of a large dose of CF, 5-FU, and CDDP as chemotherapy treatment of advanced esophageal cancer can produce promising short-term results and decrease adverse reactions.
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- 2017
13. Contrast-enhanced transrectal ultrasound for prediction of prostate cancer aggressiveness: The role of normal peripheral zone time-intensity curves
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Ling-Shan Chen, Ming Zhao, Li-Ping Yin, Hong-Bo Li, Hui Huang, Yang Zhang, Zheng-Qiu Zhu, and Zheng-Guo Zhou
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Male ,030232 urology & nephrology ,Contrast Media ,Malignancy ,Article ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Prostate ,Image Processing, Computer-Assisted ,medicine ,Humans ,Ultrasonography, Interventional ,Aged ,Multidisciplinary ,Receiver operating characteristic ,business.industry ,Ultrasound ,Area under the curve ,Prostatic Neoplasms ,Reproducibility of Results ,Cancer ,Nodule (medicine) ,Middle Aged ,Image Enhancement ,medicine.disease ,medicine.anatomical_structure ,ROC Curve ,030220 oncology & carcinogenesis ,Neoplasm Grading ,medicine.symptom ,Nuclear medicine ,business - Abstract
To assess the role of time-intensity curves (TICs) of the normal peripheral zone (PZ) in the identification of biopsy-proven prostate nodules using contrast-enhanced transrectal ultrasound (CETRUS). This study included 132 patients with 134 prostate PZ nodules. Arrival time (AT), peak intensity (PI), mean transit time (MTT), area under the curve (AUC), time from peak to one half (TPH), wash in slope (WIS) and time to peak (TTP) were analyzed using multivariate linear logistic regression and receiver operating characteristic (ROC) curves to assess whether combining nodule TICs with normal PZ TICs improved the prediction of prostate cancer (PCa) aggressiveness. The PI, AUC (p p = 0.011 and 0.040 respectively) values of the malignant nodules were significantly higher than those of the benign nodules. Incorporating the PI and AUC values (both, p p = 0.076 and 0.159 respectively), significantly improved the AUC for prediction of malignancy (PI: 0.784–0.923; AUC: 0.758–0.891) and assessment of cancer aggressiveness (p
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- 2016
14. Gene expression profile of human esophageal squamous carcinoma cell line TE-1
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Hong-Xing, Cai, Zheng-Qiu, Zhu, Xiao-Ming, Sun, Zhou-Ru, Li, Yan-Bo, Chen, and Guo-Kai, Dong
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Original Article - Abstract
Esophageal squamous cell carcinoma (ESCC) is one of the most common and deadly causes of cancer worldwide. However, to date, the mechanisms underlying its pathogenesis remain unclear. The present study investigated the gene expression profile of human esophageal cancer cell line TE-1, a cell model for ESCC, to gain insight to the genetic regulation of this disease. Human esophageal cancer TE-1 cells and normal esophageal HET-1A cells were cultured for isolation of total RNA. Differential expression of RNA transcripts was assessed using the Agilent 4×44 K microarray, combined with real-time PCR (qRT-PCR) for validation. Classification and function of the differential genes were illustrated by bioinformatics processing including hierarchical clustering and gene ontology (GO) analysis. We identified 4,986 transcripts with differential expression (fold-change ≥1.5, P
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- 2015
15. Reversion of multidrug resistance by SKI-II in SGC7901/DDP cells and exploration of underlying mechanisms
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Zheng-Qiu Zhu, Hong-Xing Cai, Ying Liu, and Zu-An Zhu
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Cancer Research ,Epidemiology ,Blotting, Western ,Reversion ,Down-Regulation ,Antineoplastic Agents ,Apoptosis ,Biology ,Flow cytometry ,Immunoenzyme Techniques ,Stomach Neoplasms ,Cell Line, Tumor ,medicine ,Humans ,MTT assay ,Cell Proliferation ,bcl-2-Associated X Protein ,Cisplatin ,medicine.diagnostic_test ,Cell growth ,Public Health, Environmental and Occupational Health ,NF-kappa B ,Molecular biology ,Drug Resistance, Multiple ,Blot ,Thiazoles ,Oncology ,Proto-Oncogene Proteins c-bcl-2 ,Cell culture ,Drug Resistance, Neoplasm ,Immunology ,medicine.drug - Abstract
In order to investigate whether SKI-II could reverse drug resistance and its possible mechanisms, we treated SGC7901/DDP cells with SKI-II or SKI-II in combination with DDP. Then cell growth, apoptosis, micro- morphological changes, and expression of SphK1, P-gp, NF-kB, Bcl-2 and Bax were assessed by MTT assay, flow cytometry, electron microscopy, immunocytochemistry and Western blot assay respectively. SGC7901/DDP cells were insensitive to cisplatin 2.5 mg/L, but when pretreated with SKI-II, their proliferation was inhibited by cisplatin 2.5mg/L significantly, the inhibition rate increasing with time and dose. The apoptosis rate was also significantly elevated. Expression of SphK1 and P-gp was decreased significantly, Pearson correlation analysis showing significant correlation between the two (r=0.595, P
- Published
- 2012
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