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Edge computing brings data computation and storage closer to the mobile device to save response time for decision making. After being processed at the edge, commonly, the data will be uploaded to the cloud for further enriched analysis. For privacy concerns, local devices may encrypt the collected data before sending it to the cloud server. However, this treatment increases the servers processing time and makes it hard to pick out the desired data. In this paper, to address this problem, we design an encrypted data validation scheme, which enables the edge to clean the encrypted data to be uploaded. Because edge computing encompasses numerous edge devices from different service providers, we explore the public-key cryptographic mechanism to implement our secure data validation scheme. Considering potential risks from quantum computers, we propose to leverage ideal lattice to realize our protocol, which reaches better performance in both time and storage for edge devices. Extensive evaluation results show that our proposed proposal achieves considerable performance improvements in terms of communication and computation aspects. Particularly, compared to prior work, nearly 123x-238x speed up is achieved in our key derivation procedure and the storage cost of the secret key is reduced from 547MB to 1.7MB.

Five new species belonging to the Chrysotus laesus-group redefined by Wei and Zhang are described and illustrated, and the group is divided into 5 species-subgroups: adunatus-subgroup, brevicercus-subgroup, nudisetus-subgroup, laesus-subgroup, trapezinus-subgroup. A key to the Chrysotus species groups known from China and all 22 species assigned to Ch. laesus species-group are provided. The type specimens of the new species are deposited in the Anshun Centre for Disease Prevention and Control, Anshun, Guizhou (ACDPCAG).http://www.zoobank.org/urn:lsid:zoobank.org:author:1B625D8E-6BAB-4A49-B49A-4F7303101DAA

Based on the strategy of copolymerization, methacrylatoethyl trimethyl ammonium chloride (DMC) and acrylamide (AM) copolymer hydrogel particles (PCM HPs) were prepared by precipitated droplets in-situ cross-linking polymerization, to overcome the easily fragile property of pure PDMC HPs and the low adsorption capacity of pure PAM HPs. Corn oil and deionized water were used for the reaction, which would not cause any pollutions; and the preparation process only took 40 s. Through the studies on various feeding ratios of DMC to AM, the hydrogel particles (PCM5-1 HPs) with the mass ratio of 5:1, showed the best mechanical strength and adsorption capacity. The compression strength of the PCM5-1 HPs was 207.7 K Pa, and no obvious mechanical loss occurred during five compression-rebound cycles. In addition, the removal ratios of anionic dyes such as amaranth red, orange G, and methyl orange were all higher than 94% (the initial dye concentration was 100 μmol/L and the adsorbent mass was 4 mg); and the adsorption capacity of orange G could reach as high as 992.63 mg/g. Meanwhile, the PCM5-1 HPs exhibited excellent selective adsorption of anionic dyes in mixed dye solutions. Therefore, the hydrogel particles prepared by the fast and environmental-friendly method had both mechanical strength and ultrahigh adsorption capacity, which verified their practicality in the removal of anionic dyes in sewage.

Inflammatory response is essential to host defense and repair, and requires tight regulation as excessive and constant inflammatory response is deleterious. We recently identified that one of the general but key mechanisms for inflammatory gene transcription regulation is controlled by the formation of super enhancers mediated by NF-κB, and bromodomain and extraterminal (BET) proteins. Given that microRNA transcription shares a similar mechanism to mRNA, we assume that the inflammatory microRNAs transcription could be NF-κB and BET bromodomain dependent. In the present study, we confirmed that inflammatory stimuli changed human umbilical vein endothelial cells (HUVEC) microRNA profile. Among these microRNAs, miR-146a and miR-155, two well-established inflammatory microRNAs, are both downregulated at transcriptional level by NF-κB and BET bromodomain inhibition. To pursue this mechanism, we analyzed the ChIP-seq data and found that NF-κB, BRD4 and RNA POL II were rapidly distributed at the upstream regions of miR-146a and miR-155, and more importantly mediated the formation of the super enhancers that drive miR-146a and miR-155 transcription. These microRNAs transcription driven by super enhancers in turn downregulate both in vitro and in vivo canonical inflammatory genes expression through targeting inflammatory mediators. This novel finding demonstrated how the host self-regulates inflammatory genes expression at both transcriptional and post-transcriptional level to ensure the appropriate level of the host inflammatory response.

The dolichopodid genus Chrysotus Meigen from China is reviewed by defining of a new species group, the Chrysotus papuanus with 107 species, along with descriptions of 105 new species. A key to the other Chrysotus species groups and the species constituting the papuanus species group is provided. The voucher specimens are deposited in the Centre for Disease Prevention and Control of Anshun City, Guizhou, China.http://zoobank.org/urn:lsid:zoobank.org:pub:4395C0CE-F96A-411A-87B7-FDC28ADA512F

To explore the effect of ROCK inhibitor Y-27632 on the matrix metalloproteinase 2 and 9 (MMP2 and MMP9) gene expression and activity in tumor necrosis factor α (TNF-α)-treated human umbilical vein endothelial cell (HUVEC). HHUVEC was divided into 3 groups, a control group, a TNF-α group, and a TNF-α plus Y-27632 group. The expressions of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), MMP2 and MMP9 were examined by real-time PCR. The MMP2/9 activity was measured by gelatin zymography. Compared to the control group, the mRNA expressions of ICAM-1, VCAM-1, MMP2 and MMP9 were increased TNF-α-treated cells, which were suppressed by ROCK inhibitor (P0.01). The MMP2/9 activity was elevated in TNF-α-treated cells, which was reversed by ROCK inhibitor (P0.05). ROCK inhibitor can suppress TNF-α-induced inflammation in endothelial cells through down-regulation of MMP2/9.目的：探讨ROCK通路抑制剂Y-27632对肿瘤坏死因子α(tumor necrosis factor α，TNF-α)诱导人脐静脉内皮细胞(human umbilical vein endothelial cell，HUVEC)基质金属蛋白酶2和9(matrix metalloproteinase 2 and 9，MMP2，MMP9)的表达与活性的影响。方法：体外培养原代HUVEC，分别予以TNF-α (25 ng/mL)、TNF-α+Y-27632(10 μmol/L)处理24 h。 Real-time PCR检测血管细胞黏附分子-1(vascular cell adhesion molecule-1，VCAM-1)、细胞间黏附分子-1(intercellular adhesion molecule-1，ICAM-1)、MMP2和MMP9 mRNA的表达水平；明胶酶谱检测MMP2和MMP9蛋白活性的改变情况。结果：与对照组相比，TNF-α明显上调ICAM-1，VACAM-1，MMP2，MMP9 mRNA的表达(P0.01)和MMP2，MMP9的蛋白活性(P0.05)；与TNF-α处理组相比，Y-27632可显著抑制ICAM-1，VCAM-1，MMP2和MMP9 mRNA的表达(P0.01)，下调MMP2和MMP9的蛋白活性(P0.05)。结论：Y-27632可以抑制TNF-α诱导的HUVEC炎症反应和MMP2，MMP9 mRNA和蛋白活性水平。.

Transcriptional coactivator with PDZ-binding motif (TAZ) is a key transcriptional mediator of Hippo signaling that has been recently reported to mediate Wnt-activated transcription and serve as a component to suppress canonical Wnt/β-catenin activity. The Bromodomain and Extra-terminal domain (BET) family of proteins can recognize the acetylated lysine chain on histones and plays a critical role in transcriptional regulation. However, the mechanisms underlying transcriptional repression by the BET bromodomain are poorly understood. Here, we found that BET bromodomain inhibition upregulated TAZ protein and its transcriptional output, independent of its well-established role as a mediator of Hippo and Wnt signaling. Additionally, JQ1, a synthetic BET inhibitor, suppressed Wnt/β-catenin activity by upregulating TAZ. Although JQ1 upregulated TAZ, which is known to promote cell proliferation, it drastically suppressed the growth of colon cancer cells by inducing cell cycle arrest. Collectively, our study identified an unexpected transcriptional repression function of the BET bromodomain and a novel mechanism for TAZ upregulation.

Wnt signaling plays essential role in mesenchymal stem cell (MSC) differentiation. Activation of Wnt signaling suppresses adipogenesis, but promotes osteogenesis in MSC. Adenomatous polyposis coli (APC) is a negative regulator of β-catenin and Wnt signaling activity. The mutation of APC gene leads to the activation of Wnt signaling and is responsible for tumorigenesis in APC(min) mouse; however, very few studies focused on its metabolic abnormalities. The present study reports a widespread metabolic disorder phenotype in APC(min) mice. The old APC(min) mice have decreased body weight and impaired adipogenesis, but severe hyperlipidemia, which mimic the phenotypes of Familial Adenomatous Polyposis (FAP), an inherited disease also caused by APC gene mutation in human. We found that the expression of lipid metabolism and free fat acids (FA) use genes in the white adipose tissue (WAT) of the APC(min) mice is much lower than those of control. The changed gene expression pattern may lead to the disability of circulatory lipid transportation and storage at WAT. Moreover, the APC(min) mice could not maintain the core body temperature in cold condition. PET-CT determination revealed that the BAT of APC(min) mice has significantly impaired ability to take up (18)FDG from the blood. Morphological studies identified that the brown adipocytes of APC(min) mice were filled with lipid droplets but fewer mitochondria. These results matched with the findings of impaired BAT function in APC(min) mice. Collectively, our study explores a new mechanism that explains abnormal metabolism in APC(min) mice and provides insights into studying the metabolic disorders of FAP patients.

With the rapid development of highway, the pollution on the surrounding water environment becomes more and more serious. In order to make a objective and effective comprehensive assessment on highway operation's impact on the surrounding water environment, we must set up an integrated quantitative analysis system of highway's impact on water environment and establish a scientific quantitative analysis model. In view of the complexity of highway water environment, this paper establishes a series of quantitative analysis models for various water environment and an comprehensive analysis model.