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CuCr contact material has become the preferred contact material for vacuum interrupters, and its composition design is an effective way to improve the microstructure and properties of the alloy. Based on the process of preparing CuCr alloy by self-propagating metallurgy, in-situ microalloying of Mo in CuCr alloy by aluminothermic self-propagating reduction is proposed in this paper. It is found that the addition of Mo can effectively reduce the Gibbs mixing free energy Gm of CuCr melt and the Gibbs mixing free energy change ΔGm of melt before and after liquid phase decomposition. The decrease of Gm can reduce the temperature of liquid phase decomposition, and the decrease of ΔGm can reduce the driving force of liquid phase decomposition and inhibit the liquid phase decomposition. On this basis, homogeneous CuCr48.5Mo0.6 alloy is successfully synthesized. whose microstructure exists in the form of two phases. Mo is mainly combined with Cr in CuCr alloy, and the addition of Mo promotes the irregular development of Cr-rich alloy. Solution + aging treatment can promote the refinement of CrMo phase, and at the Cu-rich matrix is dispersed with nano-scale fine Cr precipitates, which plays a role in strengthening the second phase. Therefore, the conductivity of CuCrMo alloy is increased from 12.63 MS m−1 to 17.41 MS m−1. Based on the performance of 12 kV vacuum interrupter assembled with self-propagating CuCr contacts, it is found that compared with CuCr alloy, the insulation, chopping current and breaking performance of the interrupter are obviously improved by adding Mo.

CuW composites are widely used in high voltage transmission, electronic information, aerospace and other cutting-edge fields. At present, the preparation methods of CuW composites based on powder metallurgy have the defects of uneven microstructure and low density. Reducing the size of tungsten particles in the CuW composites can significantly improve the microstructure uniformity and density. In this paper, an innovative idea for regulating the microstructure of CuW composites by aluminothermic coupling with magnesiothermic reduction was proposed. The thermodynamics and dynamics of the reaction system for preparation of CuW composites by this method were studied, and the microstructure of CuW composites were systematically analysed and evaluation. The researches show that the inclusion in CuW prepared by aluminothermic reduction was Al2O3, and the inclusion in CuW prepared by aluminothermic coupling with magnesiothermic reduction was MgO·Al2O3. The addition of magnesium to the reducing agent had an obvious refining effect on the tungsten particles. The mechanism was that magnesium was converted into magnesium vapor during the rapid heating process of the system, and the gas-solid, gas-liquid-solid reaction occurred with tungsten oxide to generate smaller tungsten particles. With an increase of magnesium content, the average size of tungsten particles in the microstructure of CuW gradually decreased from 2.38 μm to 0.82 μm, and the microstructure uniformity increased from 56.26% to 76.96%.

Abstract Chronic allodynia stemming from peripheral stump neuromas can persist for extended periods, significantly compromising patients’ quality of life. Conventional managements for nerve stumps have demonstrated limited effectiveness in ensuring their orderly termination. In this study, we present a spatially confined conduit strategy, designed to enhance the self-organization of regenerating nerves after truncation. This innovative approach elegantly enables the autonomous slowing of axonal outgrowth in response to the gradually constricting space, concurrently suppressing neuroinflammation through YAP-mediated mechanotransduction activation. Meanwhile, the decelerating axons exhibit excellent alignment and remyelination, thereby helping to prevent failure modes in nerve self-organization, such as axonal twisting in congested regions and overgrowth beyond the conduit’s capacity. Additionally, proteins associated with mechanical allodynia, including TRPA1 and CGRP, exhibit a gradual reduction in expression as spatial constraints tighten, a trend inversely validated by the administration of the YAP-targeted inhibitor Verteporfin. This spatially confined conduit strategy significantly alleviates allodynia, thus preventing autotomy behavior and reducing pain-induced gait alterations.

Abstract In recent years, large-scale artificial intelligence (AI) models have become a focal point in technology, attracting widespread attention and acclaim. Notable examples include Google’s BERT and OpenAI’s GPT, which have scaled their parameter sizes to hundreds of billions or even tens of trillions. This growth has been accompanied by a significant increase in the amount of training data, significantly improving the capabilities and performance of these models. Unlike previous reviews, this paper provides a comprehensive discussion of the algorithmic principles of large-scale AI models and their industrial applications from multiple perspectives. We first outline the evolutionary history of these models, highlighting milestone algorithms while exploring their underlying principles and core technologies. We then evaluate the challenges and limitations of large-scale AI models, including computational resource requirements, model parameter inflation, data privacy concerns, and specific issues related to multi-modal AI models, such as reliance on text-image pairs, inconsistencies in understanding and generation capabilities, and the lack of true “multi-modality”. Various industrial applications of these models are also presented. Finally, we discuss future trends, predicting further expansion of model scale and the development of cross-modal fusion. This study provides valuable insights to inform and inspire future future research and practice.

Abstract Background Percocypris pingi (Tchang) was classified as Endangered on the Red List of China′s Vertebrates in 2015 and is widely distributed in the Upper Yangtze River. Although breeding and release into wild habitats have been performed for this commercially important fish in recent years, low genetic diversity has been found in wild populations. Genomic resources are strongly recommended before formulating and carrying out conservation strategies for P. pingi. Thus, there is an urgent need to conserve germplasm resources and improve the population diversity of P. pingi. To date, the whole genome of P. pingi has not been reported. Results In our study, we constructed the first chromosome-level genome of P. pingi by high-throughput chromosome conformation capture (Hi-C) technology and PacBio long-read sequencing. The assembled genome was 1.7 Gb in size, with an N50 of 17,692 bp and a GC content from circular consensus sequencing of 37.67%. The Hi-C results again demonstrated that P. pingi was tetraploid (n = 98), with the genome consisting of 24-type and 25-type chromosomes. Chr.19 of the 24-type chromosomes in P. pingi resulted from the fusion of chr.19 and chr.22 in zebrafish. The divergence times between 24-type and 25-type chromosomes was around 6.1 million years ago. A total of 25,198 and 25,291 protein-coding genes were obtained from the 24-type and 25-type chromosomes, respectively. The ploidy of P. pingi is an allotetraploid. A total of 8,741 genes of P. pingi were clustered into 4,378 gene families that were shared with 14 other species, and the P. pingi genome had 68 unique gene families. Phylogenetic analyses indicated that P. pingi was most closely related to Schizothorax oconnori, and the genes were clustered on one branch. We identified 166 significantly expanded gene families and 173 significantly contracted gene families in P. pingi. The most enriched positive protein-coding genes, such as Bmp-4, Etfdh, homeobox protein HB9, and ATG3, were screened. Conclusion Our study provides a high-quality chromosome-anchored reference genome for P. pingi and provides sufficient information on the chromosomes, which will lead to valuable resources for genetic, genomic, and biological studies of P. pingi and for improving the genetic diversity, population size, and scientific conservation of endangered fish and other key cyprinid species in aquaculture.

Abstract Background Complications from prolonged esophageal foreign body impaction are well-documented, yet the significance of non-foreign body factors has not been thoroughly explored. This study aims to investigate non-foreign body risk factors for complications during esophageal foreign-body removal and to evaluate the impact of treatment timing. Study We conducted a retrospective evaluation of patients diagnosed with esophageal foreign bodies requiring gastroscopic removal in our hospital between January 2019 and December 2020. Non-foreign body factors, such as whether endoscopic treatment was on the day of the visit, visiting time, complaint time (from ingestion to presentation), anesthesia method, and holidays, were considered. Results In total, 831 patients were included. The success rate of endoscopic treatment was 97.8%. The overall probabilities of mucosal injury, bleeding, and perforation were 90.3%, 53.3%, and 6.9%, respectively. The treatment was performed on the day of the patient’s visit for 70.4% patients, under sedation anesthesia for 50.7% patients, and in the early night for 44.6% patients. Treatment on the day of the visit did not affect the success rate. Same-day treatment was a protective factor for mucosal injury and perforation on univariate logistic regression analysis, but did not independently influence mucosal injury, bleeding, or perforation on multivariate analysis. Visiting time, complaint time, and holidays affected the complication rate. During the COVID-19 period in China, visiting time and anesthesia method were found to be independent predictors of same-day treatment. Conclusions Complaint time, visiting time and same-day treatment are significant and practical factors influencing the complications of endoscopic foreign-body removal. Certain patients, notably night-time visitors, might benefit from delaying treatment until the following day to utilize sedative anesthesia to minimize risks.

Abstract Color centers in silicon carbide (SiC) offer exciting possibilities for quantum information processing. However, the challenge of ionization during optical manipulation leads to charge variations, hampering the efficacy of spin-photon interfaces. Recent research predicted that modified divacancy color centers can stabilize their charge states, resisting photoionization. This study presents a method for precisely creating single divacancy arrays in 4H-SiC using a focused helium ion beam. Photoluminescence tests reveal consistent emission with minimal linewidth fluctuations (∼50 MHz over 3 h). By measuring the ionization rate for different polytypes of divacancies, we found that the modified divacancies are more robust against resonant excitation. Furthermore, angle-resolved photoluminescence excitation spectra unveil two resonant-transition lines with orthogonal polarizations. Enhanced optical and spin characteristics were notably observed in these color centers compared to those generated through carbon-ion and shallow implantation methods, positioning modified divacancies as promising contenders for advancing quantum networking.

Abstract The application of terahertz technology in space is frontier for the development of 6G technologies. Terahertz transceiver devices based on gallium arsenide Schottky barrier diodes (GaAs SBDs) have the characteristics of small size, light weight and low power consumption, making them suitable for application on spacecraft. However, there is currently a lack of experimental assessments on their space adaptability. Here, we study the radiation hardness of terahertz devices to determine their adaptability in complex space environments. We exposed GaAs SBDs and terahertz multipliers as typical terahertz devices to gamma rays and protons. The experimental results showed that the terahertz devices exhibited good tolerance to protons, but prolonged exposure to gamma rays could significantly increase the leakage current of the GaAs SBDs and alter its C-V characteristics, leading to the failure of the terahertz multiplier. Nevertheless, the terahertz devices maintained a good level of radiation hardness, making them highly suitable for use in Low Earth Orbit (LEO) satellites. The comparison between the results of proton and gamma ray tests indicated that the terahertz devices exhibited high inherent radiation hardness against displacement damage but were more sensitive to ionization damage, requiring higher shielding requirements.

Abstract Background To investigate the effect of different working periods on missed diagnoses in patients with colorectal polyps in colonoscopy. Methods We conducted a retrospective analysis of patients who were diagnosed with colorectal polyps during colonoscopy in an outpatient department between July and December 2022. These patients were subsequently hospitalized for resection during this period. Patients with missed diagnoses were those who had newly discovered polyps in a second colonoscopy. The working periods were categorized as work, near the end of work, and delayed work, respectively, in the morning and afternoon. Results A total of 482 patients were included, and the miss rate of diagnosis was 48.1% (232/482), mainly in the transverse colon (25%), and the ascending colon (23%). Patient age was a risk factor for the miss rate of diagnosis (OR = 1.025, 95%CI: 1.009–1.042, P = 0.003) and was also associated with the number of polyps detected for the first colonoscopy (χ2 = 18.196, P = 0.001). The different working periods had no statistical effect on the missed rate of diagnosis (χ2 = 1.998, P = 0.849). However, there was an increasing trend in miss rates towards the end of work and delayed work periods, both in the morning and afternoon. The highest miss rate (60.0%) was observed during delayed work in the afternoon. Additionally, poor bowel preparation was significantly more common during delayed work in the afternoon. Conclusions The increasing trend in miss rates towards the end of work and delayed work periods deserves clinical attention. Endoscopists cannot always stay in good condition under heavy workloads.

Alternaria species are commonly found as saprophytes, endophytes and plant pathogens. During a survey of small-spored Alternaria in China, two new species were discovered from Cucurbitaceae plants collected in Hubei and Sichuan provinces. This study identified two new species of Alternaria using seven genes (ITS, GAPDH, TEF1, RPB2, Alt a 1, EndoPG, and OPA10-2) for phylogenetic analyses and morphological characteristics. The two new species A. jingzhouensis and A. momordicae were described and illustrated. Alternaria jingzhouensis sp. nov., associated with Citrullus lanatus, is characterized by producing muriform, ellipsoidal, flask-shaped, rostrate, and beaked conidia. It differs from A. koreana, A. ovoidea, and A. baoshanensis by bearing conidia in a simple conidiogenous locus with occasionally longer beaks in a chain, and from A. momordicae sp. nov. by having shorter beaks. Alternaria momordicae sp. nov. from Momordica charantia was distinct from A. koreana, A. ovoidea, and A. baoshanensis by producing muriform, long ellipsoid or ovoid to obclavate, sometimes inverted club-shaped conidia on a single conidiogenous locus with a wider body and longer beak in a chain, and distinct from A. jingzhouensis sp. nov. by a longer beak conidia. These two species were clearly distinguished from other species in the section Alternaria based on DNA based phylogeny and morphological characteristics. The morphological features were discussed and compared to relevant species in the present paper.

Abstract Background Cyp19a1a is a key enzyme in the pathway that converts androgens into estrogen and is regulated by TGF-β signaling. Smad4 and FoxH1 are downstream effectors of TGF-β signaling and may play important roles in ovarian development in M. albus. Methods We investigated the expression pattern of the Smad4 and FoxH1 using qRT‒PCR and immunofluorescence, then tested the changes of smad4 and foxh1 by qRT‒PCR after ovary incubation with FSH in vitro, and analysed the regulation of cyp19a1a transcription by Smad4 and FoxH1 by dual-luciferase reporter assays. Results We found that Smad4 encoded a putative protein of 449 amino acids and harbored the three conserved domains typical of this protein family. Smad4 and foxh1 exhibited similar expression patterns during ovarian development and after FSH incubation, with Pearson’s coefficients of 0.873 and 0.63–0.81, respectively. Furthermore, Smad4, FoxH1 and Cyp19a1a colocalized in the granulosa cells and theca cells of ovaries during the mid-to-late vitellogenic stage. Smad4 repressed cyp19a1a activity via SBE1 (− 1372/−1364) and SBE2 (− 415/−407) in the cyp19a1a promoter, whereas mutating SBE1 or SBE2 restored cyp19a1a promoter activity. Co-overexpression of Smad4 and FoxH1 significantly reduced cyp19a1a promoter activity. Conclusions This study provides new insights into the potential functions of transcription factors Smad4 and FoxH1 in ovarian development and the transcriptional regulation mechanism of cyp19a1a in M. albus, which will reveal Smad4/FoxH1-mediated TGF-β signaling in reproduction and the regulation of the cyp19a1a. Plain English summary Aromatase, encoded by cyp19a1a, is involved in ovarian development and plays an important role in the quality of eggs, as well the sex ratio, of the teleost fish, M. albus. The research on the transcriptional regulation of cyp19a1a has contributed to the understanding of its role in ovarian development. In previous study, it was shown that FoxH1 inhibits cyp19a1a transcription. In the present study, Smad4 was confirmed as a cyp19a1a transcriptional repressor and Smad4 may also coordinate with FoxH1 to repress cyp19a1a transcription. At present, we provide a new perspective for the transcriptional regulation of cyp19a1a by transcription factors Smad4 and FoxH1 in teleost fish ovary. In the future, the regulatory networks of Smad4 and FoxH1 will be further studied and the gene editing technology will be applied to screen specific regulatory factors of cyp191a1a gene, so as to alter the female cycle and modulate the sex ratio of the eggs production.

A method combining experimental and simulation optimization was used to calibrate parameters to enhance the accuracy of discrete element model parameters during kiwifruit stem separation. First, physical experiments were conducted to determine the intrinsic and contact parameters of kiwifruit stalk. Second, the mechanical parameters of the kiwifruit stalks were determined using three-point bending and shear tests. On this basis, simulation tests were conducted on kiwifruit stalks by combining the Hertz-Mindlin model with a bonding model, and the optimal combination of bonding parameters was confirmed using the bending strength and maximum shear force. Finally, a discrete element model of the kiwifruit was built with the determined bonding parameters and simulated, and the reliability of the model was verified through mechanical tests. The results showed that the density was 867.5 kg/m3, Poisson's ratio was 0.26, the modulus of elasticity was 3.25 × 108 Pa, the recovery coefficient between the fruit stalks and steel parts was 0.365, and the average values of the static and dynamic friction coefficients between the kiwifruit stalks and steel parts were 0.268 and 0.152, respectively. The kiwifruit stem bonding parameters were normal stiffness per unit area kn=7.201×1011 N/m3, shear stiffness per unit area kt=2.379×1011 N/m3, critical normal stress σmax=5.937×108 Pa, critical shear stress tmax = 2.354×109 Pa, and bonded disc radius Rj=0.164 mm. Compared with the results of the mechanical tests, the relative errors of the bending strength and maximum shear of the discrete element model were 2.13% and 2.84%, respectively. The results showed that the discrete element model improves the simulation of the bending and shearing processes of kiwifruit stalks and is capable of characterizing the physical properties of kiwifruit stalks. The results of this study provide a theoretical foundation for the optimal design of end effectors.

ABSTRACTWith the development of social media, it has become increasingly important to quickly and accurately identify social media texts related to disasters (e.g. typhoon) to aid in rescue and recovery efforts. Currently, multi-class classification and pre-trained language model Bidirectional Encoder Representations from Transformers (BERT) are widely used for text classification. However, most studies on typhoon damage classification are multi-class single-label, which contradicts to the reality that a social media text may correspond to multiple types of damage. Moreover, the outputs of the hidden layers of BERT are not fully utilized. This paper proposes a two-stage multi-class multi-label classification method for typhoon damage assessment by fully integrating the outputs of the hidden layers of BERT. In the first stage, sentence vectors are adopted to identify typhoon damage-related texts. In the second stage, word matrices are applied for multi-class multi-label classification to further classify the texts into five damage categories (i.e. transportation, public, electricity, forestry, and waterlogging). The two stages are trained end-to-end to identify typhoon damage from social media texts. Experiments on [Formula: see text] texts during typhoon landfall in Chinese coastal regions demonstrate that the proposed method can effectively improve the accuracy of text classification and comprehensively assess typhoon damage.

Objectives Persistent severe acute kidney injury (PS-AKI) is associated with poor clinical outcomes. Our study attempted to evaluate the diagnostic value of chemokines for early-stage PS-AKI prediction.Methods According to the KDIGO criteria, 115 COVID-19 patients diagnosed with stage 2/3 AKI were recruited from the intensive care unit between December 2022 and February 2023. Primary clinical outcomes included detecting PS-AKI in the first week (≥ KDIGO stage 2 ≥ 72 h). Cytometric Bead Array was used to detect patient plasma levels (interleukin-8 (IL-8), C-C chemokine ligand 5 (CCL5), chemokine (C-X-C Motif) ligand 9 (CXCL9), and interferon-inducible protein 10 (IP-10)) of chemokines within 24 h of enrollment.Results Of the 115 COVID-19 patients with stage 2/3 AKI, 27 were diagnosed with PS-AKI. Among the four measured chemokines, only the IL-8 level was significantly elevated in the PS-AKI group than in the Non-PS-AKI group. IL-8 was more effective as a biomarker while predicting PS-AKI with an area under the curve of 0.769 (0.675-0.863). This was superior to other biomarkers related to AKI, including serum creatinine. Moreover, plasma IL-8 levels of >32.2 pg/ml on admission could predict PS-AKI risk (sensitivity = 92.6%, specificity = 51.1%). Additionally, the IL-8 level was associated with total protein and IL-6 levels.Conclusion Plasma IL-8 is a promising marker for the early identification of PS-AKI among COVID-19 patients. These findings should be validated in further studies with a larger sample size.

Small mothers against decapentaplegic2/3 (Smad2/3) are two signalling intermediates in the transforming growth factor beta (TGF-β) signalling pathway, which plays a critical role in gonad development. However, a systemic analysis of Smad2/3 in Monopterus albus (M. albus) has not been performed. The smad2 sequence in M. albus was characterized in our previous publication. In this study, we characterized the nucleotide sequence of smad3 and reported that smad3 is widely expressed in different tissues of M. albus. Importantly, the expression of smad3 significantly increased at the early vitellogenic stage in the ovary and decreased with oocyte maturation. Next, we aimed to identify the upstream factors that regulate Smad2/3 transcription. The 5′ flanking sequences of smad2/3 were cloned, and their core promoter regions were determined to be between −447 bp and −100 bp and between −1520 bp and −927 bp, respectively. The Jun and Foxo1 binding sites were conserved in the smad2 promoter region, and the Sp1, Bcl-6, and Nobox binding sites were conserved in the smad3 promoter region. Through dual-luciferase reporter gene experiments, we showed that Jun and Foxo1 binding sites may be involved in the regulation of smad2 transcription, whereas the Nobox binding site may be involved in the activation of the smad3 promoter.

Large-volume presses (LVPs) providing large volumes, liquid media, deformation capability, jump compression, and in situ measurements are in great demand for high-pressure research, particularly in the fields of geoscience, condensed matter physics, material science, chemistry, and biology. A high-pressure and high-temperature (HPHT) platform with different LVP subsystems, both solid-state and liquid environments, and nonequilibrium subsystems, has been constructed at the Synergetic Extreme Condition User Facility, Jilin University. This article describes the construction of the different subsystems and provides an overview of the capabilities and characteristics of the different HPHT subsystems. A large sample volume (1000 mm3) at 20 GPa is achieved through the use of a belt-type apparatus in the solid-state subsystem. HPHT conditions (1.8 GPa and 1000 K) are realized in the liquid subsystem through the use of a piston–cylinder-type LVP with optical diamond windows for in situ spectroscopic measurements. A maximum pressure jump to 10.2 GPa can be reached within 20 ms in the nonequilibrium subsystem with the use of an improved bladder-pressurization jump press. Some typical results obtained with different LVPs are briefly reviewed to illustrate the applications and advantages of these presses. In summary, the platform described here has the potential to contribute greatly to high-pressure research and to innovations in high-pressure technology.

Abstract Background The ricefield eel Monopterus albus undergoes a natural sex change from female to male during its life cycle, and previous studies have shown the potential mechanisms of this transition at the transcriptional and protein levels. However, the changes in protein levels have not been fully explored, especially in the intersexual stage. Results In the present study, the protein expression patterns in the gonadal tissues from five different periods, the ovary (OV), early intersexual stage gonad (IE), middle intersexual stage gonad (IM), late intersexual stage gonad (IL), and testis (TE), were determined by untargeted proteomics sequencing. A total of 5125 proteins and 394 differentially expressed proteins (DEPs) were detected in the gonadal tissues. Of the 394 DEPs, there were 136 between the OV and IE groups, 20 between the IM and IE groups, 179 between the IL and IM groups, and 59 between the TE and IL groups. Three candidate proteins, insulin-like growth factor 2 mRNA-binding protein 3 isoform X1 (Igf2bp3), triosephosphate isomerase (Tpi), and Cu-Zn superoxide dismutase isoform X1 [(Cu-Zn) Sod1], were validated by western blotting to verify the reliability of the data. Furthermore, metal metabolite-related proteins were enriched in the IL vs. IM groups and TE vs. IL groups, which had close relationships with sex change, including Cu2+-, Ca2+-, Zn2+- and Fe2+/Fe3+-related proteins. Analysis of the combined transcriptome data revealed consistent protein/mRNA expression trends for two metal metabolite-related proteins/genes [LOC109953912 and calcium Binding Protein 39 Like (cab39l)]. Notably, we detected significantly higher levels of Cu2+ during the sex change process, suggesting that Cu2+ is a male-related metal metabolite that may have an important function in male reproductive development. Conclusions In summary, we analyzed the protein profiles of ricefield eel gonadal tissues in five sexual stages (OV, IE, IM, IL, and TE) and verified the plausibility of the data. After preforming the functional enrichment of metal metabolite-related DEPs, we detected the contents of the metal metabolites Zn2+, Cu2+, Ca2+, and Fe2+/Fe3+ at these five stages and screened for (Cu-Zn) Sod1 and Mmp-9 as possible key proteins in the sex reversal process.

The cryopreservation of oocytes through vitrification is imperative for the conservation of livestock germplasm resources. However, as oocytes exhibit significant oxidative stress and organelle damage following vitrification freezing, it is crucial to optimise the vitrification conditions to mitigate the deleterious effects of freezing. In this study, we demonstrated that spermidine has been showed to enhance oocyte survival after vitrification freezing (92% ± 4% vs. 82% ± 3%, p < 0.01) and blastocyst formation after freezing for in vitro fertilisation (14.86% ± 7% vs. 6% ± 3, p < 0.05). Spermidine supplementation rescued 47.3% of dysregulated pathways, including ovarian steroidogenesis, and restored normal expression levels in 43.3% of aberrantly expressed genes. Subsequent studies elucidated that spermidine effectively rescued mitochondrial dysfunction after vitrification, alleviated oxidative stress damage, and regulated intracellular calcium homeostasis. Consequently, we concluded that the addition of spermidine during vitrification freezing is an effective method to protect oocytes from freezing damage.

A long noncoding RNA (lncRNA) is longer than 200 bp. It regulates various biological processes mainly by interacting with DNA, RNA, or protein in multiple kinds of biological processes. Adenosine monophosphate-activated protein kinase (AMPK) is activated during nutrient starvation, especially glucose starvation and oxygen deficiency (hypoxia), and exposure to toxins that inhibit mitochondrial respiratory chain complex function. AMPK is an energy switch in organisms that controls cell growth and multiple cellular processes, including lipid and glucose metabolism, thereby maintaining intracellular energy homeostasis by activating catabolism and inhibiting anabolism. The AMPK signalling pathway consists of AMPK and its upstream and downstream targets. AMPK upstream targets include proteins such as the transforming growth factor β-activated kinase 1 (TAK1), liver kinase B1 (LKB1), and calcium/calmodulin-dependent protein kinase β (CaMKKβ), and its downstream targets include proteins such as the mechanistic/mammalian target of rapamycin (mTOR) complex 1 (mTORC1), hepatocyte nuclear factor 4α (HNF4α), and silencing information regulatory 1 (SIRT1). In general, proteins function relatively independently and cooperate. In this article, a review of the currently known lncRNAs involved in the AMPK signalling pathway is presented and insights into the regulatory mechanisms involved in human ageing and age-related diseases are provided.

Abstract Background Schizothorax o’connori is an endemic fish distributed in the upper and lower reaches of the Yarlung Zangbo River in China. It has experienced a fourth round of whole gene replication events and is a good model for exploring the genetic differentiation and environmental adaptability of fish in the Qinghai-Tibet Plateau. The uplift of the Qinghai-Tibet Plateau has led to changes in the river system, thereby affecting gene exchange and population differentiation between fish populations. With the release of fish whole genome data, whole genome resequencing has been widely used in genetic evolutionary analysis and screening of selected genes in fish, which can better elucidate the genetic basis and molecular environmental adaptation mechanisms of fish. Therefore, our purpose of this study was to understand the population structure and adaptive characteristics of S. o’connori using the whole-genome resequencing method. Results The results showed that 23,602,746 SNPs were identified from seven populations, mostly distributed on chromosomes 2 and 23. There was no significant genetic differentiation between the populations, and the genetic diversity was relatively low. However, the Zangga population could be separated from the Bomi, Linzhi, and Milin populations in the cluster analysis. Based on historical dynamics analysis of the population, the size of the ancestral population of S. o’connori was affected by the late accelerated uplift of the Qinghai Tibet Plateau and the Fourth Glacial Age. The selected sites were mostly enriched in pathways related to DNA repair and energy metabolism. Conclusion Overall, the whole-genome resequencing analysis provides valuable insights into the population structure and adaptive characteristics of S. o’connori. There was no obvious genetic differentiation at the genome level between the S. o’connori populations upstream and downstream of the Yarlung Zangbo River. The current distribution pattern and genetic diversity are influenced by the late accelerated uplift of the Qinghai Tibet Plateau and the Fourth Ice Age. The selected sites of S. o’connori are enriched in the energy metabolism and DNA repair pathways to adapt to the low temperature and strong ultraviolet radiation environment at high altitude.

Abstract Background Whole-genome methylation sequencing of cfDNA is not cost-effective for tumor detection. Here, we introduce reduced representative methylome profiling (RRMP), which employs restriction enzyme for depletion of AT-rich sequence to achieve enrichment and deep sequencing of CG-rich sequences. Methods We first verified the ability of RRMP to enrich CG-rich sequences using tumor cell genomic DNA and analyzed differential methylation regions between tumor cells and normal whole blood cells. We then analyzed cfDNA from 29 breast cancer patients and 27 non-breast cancer individuals to detect breast cancer by building machine learning models. Results RRMP captured 81.9% CpG islands and 75.2% gene promoters when sequenced to 10 billion base pairs, with an enrichment efficiency being comparable to RRBS. RRMP allowed us to assess DNA methylation changes between tumor cells and whole blood cells. Applying our approach to cfDNA from 29 breast cancer patients and 27 non-breast cancer individuals, we developed machine learning models that could discriminate between breast cancer and non-breast cancer controls (AUC = 0.85), suggesting possibilities for truly non-invasive cancer detection. Conclusions We developed a new method to achieve reduced representative methylome profiling of cell-free DNA for tumor detection.

Energetic structural materials (ESMs) are a new type of structural materials with bearing and damage characteristics. In this work the microstructure, mechanical properties and energy release characteristics of multi-element Ti–Zr–Ta alloys with good casting performance were studied. The microstructure of the TixZrTa alloys gradually change from BCC + HCP to single BCC structure with the increase of Ti. While the Ti2ZryTa alloys was still uniform and single BCC structure with the increase of Zr. The evolution of microstructure and composition then greatly affect the mechanical properties and energy-release characteristics of Ti–Zr–Ta alloys. The synergistic effect of dual phase structure increases the fracture strain of TixZrTa (x = 0.2, 0.5) with the Ti content decreases, while the fracture strain of TixZrTa (x = 2.0, 3.0, 4.0) gradually increase with the Ti content increases caused by the annihilation of the obstacles for dislocation movement. And as Zr content increases, the fracture strain of Ti2ZryTa alloys decrease, then the oxidation reaction rate and fragmentation degree gradually increase. The higher oxidation rate and the lager exposed oxidation area jointly leads the higher releasing energy efficiency of TixZrTa alloys with low Ti content and Ti2ZryTa alloys with high Zr content.

Abstract Lightweight flexible piezoelectric polymers are demanded for various applications. However, the low instinctively piezoelectric coefficient (i.e. d33) and complex poling process greatly resist their applications. Herein, we show that introducing dynamic pressure during fabrication is capable for poling polyvinylidene difluoride/barium titanate (PVDF/BTO) composites with d33 of ~51.20 pC/N at low density of ~0.64 g/cm3. The melt-state dynamic pressure driven energy implantation induces structure evolutions of both PVDF and BTO are demonstrated as reasons for self-poling. Then, the porous material is employed as pressure sensor with a high output of ~20.0 V and sensitivity of ~132.87 mV/kPa. Besides, the energy harvesting experiment suggests power density of ~58.7 mW/m2 can be achieved for 10 N pressure with a long-term durability. In summary, we not only provide a high performance lightweight, flexible piezoelectric polymer composite towards sustainable self-powered sensing and energy harvesting, but also pave an avenue for electrical-free fabrication of piezoelectric polymers.

Background: Seminal plasma is an important component of semen and has a significant effect on sperm function. However, the relationship between seminal plasma and sperm freezing capacity has not been fully studied. Purpose: Exploring metabolites and proteins related to the boar sperm freezing capacity in seminal plasma, by metabolomic and proteomic approaches, and directly verifying the protective effect of seminal plasma on the cryopreservation of boar sperm using high and low freezability seminal plasma as base freezing extender. Methods: Semen samples were collected from 30 different boars, 11 high and 11 low freezing-resistant boars were selected after freezing 2~4 times, and seminal plasma was selected at the same time. Sperm motility and movement parameters were analyzed using a CASA system. Reproductive hormones (Testosterone, progesterone, estradiol, prolactin, prostaglandin F2α, luteinoid hormone) in seminal plasma were detected by ELISA. Analysis of proteins and metabolites in high and low freezing-resistant seminal plasma by proteomics and metabolomics techniques. Results: The six reproductive hormones tested were not significantly associated with sperm freezing resistance. A total of 13 differentially expressed metabolites (DEMs) and 38 differentially expressed proteins (DEPs) were identified, while a total of 348 metabolites and 1000 proteins were identified. These DEMs were related to energy metabolism, drugs, or environmental pollutants, while the DEPs were mainly involved in the cytoskeletal dynamics and cell adhesion processes. There were 33 metabolites and 70 proteins significantly associated with mean progress motility (PM) at 10 min and 2 h after thawing. The 70 related proteins were associated with cell division and cycle regulation in gene ontology (GO) terms, as well as KEGG pathways, thermogeneration, and pyruvate metabolism. Using highly freezable boar SP as a base freezing extender made no difference from using lowly freezable boar SP, and both were not as good as the commercial control. Conclusion: There were significant differences in seminal plasma with different freezability, but the similarity was much greater than the difference. The protection effect of seminal plasma is not remarkable, and it does not exhibit superior cryoprotective properties compared to commercial semen cryoelongators. Significance: This study provides a deeper understanding of how seminal plasma composition affects sperm freezabilty. It provides potential biomarkers and targets for improving sperm cryopreservation techniques.

The retrieval of global significant wave height (SWH) data is crucial for maritime navigation, aquaculture safety, and oceanographic research. Leveraging the high temporal resolution and spatial coverage of Cyclone Global Navigation Satellite System (CYGNSS) data, machine learning models have shown promise in SWH retrieval. However, existing models struggle with accuracy under high-SWH conditions and discard a significant number of such observations due to low quality, which limits their effectiveness in global SWH retrieval, particularly for monitoring tropical cyclone (TC) events. To address this, this study proposes a daily global SWH retrieval framework through the enhanced eXtreme Gradient Boosting model (XGBoost-SC), which incorporates Cumulative Distribution Function (CDF) matching to introduce prior distribution information and reduce errors for SWH values exceeding 3 m. An enhanced loss function is employed to improve accuracy and mitigate the distribution bias in low-SWH retrieval induced by CDF matching. The results were tested over one million sample points and validated against the European Centre for Medium-Range Weather Forecasts (ECMWF) SWH product. With the help of CDF matching, XGBoost-SC outperformed all models, significantly reducing RMSE and bias while improving the retrieval capability for high SWHs. For SWH values between 3–6 m, the RMSE and bias were 0.94 m and −0.44 m, and for values above 6 m, they were 2.79 m and −2.0 m. The enhanced performance of XGBoost-SC for large SWHs was further confirmed in TC conditions over the Western North Pacific and in the Western Atlantic Ocean. This study provides a reference for large-scale SWH retrieval, particularly under TC conditions.

Abstract Background Population‐based epidemiological evidence regarding the association between fruit and vegetable intake and the incidence of hypertension is inconsistent. This prospective cohort study aimed to investigate the association between fruit and vegetable intake and the risk of new‐onset hypertension. Methods Based on the project of Prediction for Atherosclerotic Cardiovascular Disease Risk in China (China‐PAR), 58,981 Chinese adults without hypertension at baseline were included. Information on fruit and vegetable intake was collected using a food‐frequency questionnaire. Cox proportional hazards models were performed to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) of incident hypertension. Results During 640,795 person‐years of follow‐up, 21,008 new cases of hypertension were recorded. Compared with participants in the lowest quintile (Q1) of total fruit and vegetable (TFV) intake, the HRs (95% CIs) of incident hypertension were 0.90 (0.86–0.95), 0.85 (0.81–0.90), 0.82 (0.78–0.86), and 0.83 (0.78–0.88) for the Q2 to Q5 group (ptrend

The prediction of equilibrium components for chemical reactions is a considerable section in the metallurgical industry. According to the ion and molecule coexistence theory (IMCT), a modified mass action concentration model based on a thermodynamic database is proposed in this paper, which complys with the law of mass conservation and can be applied in the batching process for Al-Ti-Ca-oxide system that originates from SHS (Self-propagating High-temperature Synthesis) metallurgy. The trend for slag and alloy component under different batching conditions are in good agreement with experiment, while the difference between the theoretical calculation and experiment can be attributed to the deviation from the thermodynamic equilibrium. The modified mass action concentration model with melts and slag can be used to predict the composition and content of the system when equilibrium is achieved at a certain temperature under a specific material ratio, which is conducive to reducing the cost of the experiment and predicting the operability of the actual process. Moreover, it is believed that this thermodynamic insight may has certain application prospects in these metallurgical procedure based on the equilibrium process.

Alismatis Rhizoma (AR) is a crucial substance for discovering new triterpenoids to address hyperlipidemia and obesity. Currently, approximately 120 triterpenoids have been identified in AR, making the discovery of new triterpenoids increasingly challenging. Thus, based on the advantages of HPLC-HRMS, it was utilized to identify both reported and new triterpenoids, and then in-silico analysis was employed to predict the functions of these new triterpenoids. Twenty reported triterpenoids and four new triterpenoids (25-methoxy-16-oxo-11-anhydroalisol A, 25-methoxy-16-oxo-alisol A, 25-methoxy-16-oxo-alisol A 23-acetate, and 25-methoxy-16-oxo-alisol A 24-acetate) from AR were identified using HPLC-HRMS, and then KEGG analysis suggested four new triterpenoids might be activated the PPAR signaling pathway to relieve hyperlipemia. The results of PPI and molecular docking indicated PPARA might be the key targets for anti-hyperlipidemia of four new triterpenoids, of which 25-methoxy-16-oxo-11-anhydroalisol A has better binding activity with PPARA. Additionally, 25-methoxy-16-oxo-11-anhydroalisol A has more potential as a candidate drug because of its better absorption, distribution, metabolism, excretion, and lower toxicity. Hence, our findings proved there are new triterpenoids with anti-hyperlipidemia medicinal potential in AR, thereby guiding the direction of future works and reducing the consumption of time and financial resources.