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Background and Objectives: The main issues should be solved in Chinese hospital management process are illustrated as long registration time and waiting list, extensive prescription dealing period and short duration of General Practitioner consultation. In order to improve healthcare process and patients’ satisfactory level, a large amount of hospitals is engaging in developing mobile health applications. However, the research on the impact of mobile health applications use (MHAU) on patients’ satisfactory level and treatment quality are quite few. This research is concentrated on evaluating patients’ satisfaction and healthcare service quality via empirical analysis.Methods: We established one model based on literature review, which related to MHAU, clinical process change perceived by patient, service quality, and patient satisfaction. Additionally, the mature scale were selected and revised as a survey instrument in this study, and was conducted at Shenzhen Ophthalmic Hospital using a paper questionnaire. In terms of the survey, patients are randomly selected to fill out the form during December 2018 to January 2019. Further, the research model and hypotheses has been verified with assistance of regression analysis.Results: The results demonstrated that the MHAU on the patient’s satisfaction is significant and direct (b=.162, p=.000Conclusions: Hospital managers should not only pay attention to the management of system usage, but also to organizational factors that affect the results of system usage, such as the clinical process change perceived by patients. The dimension of convenience should be considered when evaluating the service quality of hospital, especially for large hospitals with large outpatient service. In addition, the managers should pay attention to enhancing the initiative of physician-patient interaction, as it is an important factor affecting patients' perception of the services quality. This study provides a reference for investigations in ophthalmic hospital scene and needs to be confirmed further in other hospital scenarios.

In previous work, we have shown the Sodium/Potassium/2 Chloride Cotransporter (NKCC1) to be a key effector of lens fiber cell volume regulation. Since others have shown that the activity of NKCC1 is regulated via its phosphorylation status, the purpose of this study was to investigate whether NKCC1 phosphorylation can be modulated in organ cultured bovine lenses, and to see how this relates to changes in lens wet weight. Western blotting was first used to confirm the expression of NKCC1, phosphorylated NKCC1 (NKCC1-P) and the regulatory kinases WNK/SPAK and phosphatases PP1/PP2A in bovine lenses at the protein level. Changes to NKCC1-P status were then assessed by organ culturing bovine lenses in either isotonic, hypertonic or hypotonic solutions in the presence or absence of the NKCC inhibitor, bumetanide, or phosphatase inhibitors okadaic acid and calyculin A. After 1–22 h of culturing, lenses were weighed, assessed for transparency and the cortical protein fractions analyzed by western blot using antibodies to detect total NKCC1 and NKCC1-P. NKCC1, NKCC1-P, SPAK, PP1 and PP2A were all detected in the membrane fraction of bovine lenses. Under hypertonic conditions, NKCC1 is phosphorylated and activated to mediate a regulatory volume increase. Finally, NKCC1-P signal increased in the presence of phosphatase inhibitors indicating that PP1/PP2A can dephosphorylate NKCC1. These results show that the phosphorylation status and hence activity of NKCC1 is dynamically regulated and that in response to hypertonic stress, NKCC1 activity is increased to effect a regulatory volume increase that limits cell shrinkage. These findings support the view that the lens dynamically regulates ion fluxes to maintain steady state lens volume, and suggest that dysfunction of this regulation maybe an initiating factor in the localized fiber cell swelling that is a characteristic of diabetic lens cataract.

Purpose Images from cultured lens cells do not convey enough spatial information, and imaging of fixed lens specimens cannot reveal dynamic changes in the cells. As such, a real-time, convenient approach for monitoring label-free imaging of dynamic processes of living cells within the whole lens is urgently needed. Methods Female Wistar rat lenses were kept in organ culture. Insulin-like growth factor-I was added to the culture medium to induce cell mitosis. A novel method of ultraviolet (UV) irradiation was used to induce cell apoptosis and fiber damage. The cellular morphological dynamics within the whole lens were monitored by inverted phase contrast microscopy. Apoptosis was assessed using a commercial kit with Hoechst 33342/YO-PRO®-1/propidium iodide (PI). Results The intrinsic transparency and low-light scattering property of the rat lens permitted direct imaging of the lens epithelial cells (LECs) and the superficial fiber cells. We visualized the processes of mitosis and apoptosis of the LECs, and we obtained dynamic images of posterior fiber cells following UVA irradiation. Conclusions This method opens a new window for observing lens cells in their physiologic location, and it can be readily applied in studies on lens physiology and pathology.