Search

Your search keyword '"Zhong, Xiufang"' showing total 11 results
Start Over Author "Zhong, Xiufang"
11 results on '"Zhong, Xiufang"'

3. Metabolic and epigenetic dysfunctions underlie the arrest of in vitro fertilized human embryos in a senescent-like state

Author

Yang, Yang, primary, Shi, Liyang, additional, Fu, Xiuling, additional, Ma, Gang, additional, Yang, Zhongzhou, additional, Li, Yuhao, additional, Zhou, Yibin, additional, Yuan, Lihua, additional, Xia, Ye, additional, Zhong, Xiufang, additional, Yin, Ping, additional, Sun, Li, additional, Zhang, Wuwen, additional, Babarinde, Isaac A., additional, Wang, Yongjun, additional, Zhao, Xiaoyang, additional, Hutchins, Andrew P., additional, and Tong, Guoqing, additional

Published
2022
Full Text
View/download PDF

4. Correction of the pathogenic mutation in TGM1 gene by adenine base editing in mutant embryos

Author

Dang, Lu, primary, Zhou, Xueliang, additional, Zhong, Xiufang, additional, Yu, Wenxia, additional, Huang, Shisheng, additional, Liu, Hanyan, additional, Chen, Yuanyuan, additional, Zhang, Wuwen, additional, Yuan, Lihua, additional, Li, Lei, additional, Huang, Xingxu, additional, Li, Guanglei, additional, Liu, Jianqiao, additional, and Tong, Guoqing, additional

Published
2022
Full Text
View/download PDF

5. Human embryos arrest in a quiescent-like state characterized by metabolic and zygotic genome activation problems

Author

Yang, Yang, primary, Shi, Liyang, additional, Fu, Xiuling, additional, Ma, Gang, additional, Yang, Zhongzhou, additional, Li, Yuhao, additional, Zhou, Yibin, additional, Yuan, Lihua, additional, Xia, Ye, additional, Zhong, Xiufang, additional, Yin, Ping, additional, Sun, Li, additional, Wuwen, Zhang, additional, Babarinde, Isaac A., additional, Wang, Yongjun, additional, Zhao, Xiaoyang, additional, Hutchins, Andrew P., additional, and Tong, Guoqing, additional

Published
2021
Full Text
View/download PDF

8. Correction of the pathogenic mutation in TGM1gene by adenine base editing in mutant embryos

Author

Dang, Lu, Zhou, Xueliang, Zhong, Xiufang, Yu, Wenxia, Huang, Shisheng, Liu, Hanyan, Chen, Yuanyuan, Zhang, Wuwen, Yuan, Lihua, Li, Lei, Huang, Xingxu, Li, Guanglei, Liu, Jianqiao, and Tong, Guoqing

Abstract

A couple diagnosed as carriers for lamellar ichthyosis, an autosomal recessive rare disease, encountered two pregnancy losses. Their blood samples showed the same heterozygous c.607C>T mutation in the TGM1gene. However, we found that about 98.4% of the sperm had mutations, suggesting possible de novogermline mutation. To explore the probability of correcting this mutation, we used two different adenine base editors (ABEs) combined with related truncated single guide RNA (sgRNA) to repair the pathogenic mutation in mutant zygotes. Our results showed that the editing efficiency was 73.8% for ABEmax-NG combined with 20-bp-length sgRNA and 78.7% for Sc-ABEmax combined with 19-bp-length sgRNA. The whole-genome sequencing (WGS) and deep sequencing analysis demonstrated precise DNA editing. This study reveals the possibility of correcting the genetic mutation in embryos with the ABE system.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results