1. CD54+ rabbit adipose-derived stem cells overexpressing HIF-1α facilitate vascularized fat flap regeneration
- Author
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Yi-Dan Liang, Guang-Yi Chi, Zhen-Dong Chen, Qi-Liu Peng, Rong-He Gu, Zhong-Heng Wei, Fang-Tian Xu, De-Quan Li, Guan-Ming Lu, Donghua Zou, Min-Hong Huang, Zhi-Jie Liang, Li Chen, Hui Gao, and Hong-Mian Li
- Subjects
0301 basic medicine ,engineered fat flaps ,medicine.medical_specialty ,Cellular differentiation ,Paracrine Communication ,HIF-1α ,Gene Expression ,Neovascularization, Physiologic ,Adipose tissue ,chemical and pharmacologic phenomena ,Free Tissue Flaps ,rabbit adipose-derived stem cells ,Immunophenotyping ,angiogenesis ,03 medical and health sciences ,Paracrine signalling ,hemic and lymphatic diseases ,parasitic diseases ,Animals ,Regeneration ,Medicine ,Wound Healing ,Adipogenesis ,business.industry ,Stem Cells ,Regeneration (biology) ,Cell Differentiation ,hemic and immune systems ,Hypoxia-Inducible Factor 1, alpha Subunit ,Cell biology ,Surgery ,Transplantation ,030104 developmental biology ,Adipose Tissue ,Oncology ,Models, Animal ,Rabbits ,Stem cell ,adipogenic differentiation ,business ,Biomarkers ,Research Paper - Abstract
Fat flap transplantation is frequently performed in patients suffering from soft tissue defects resulting from disease or trauma. This study explored the feasibility of constructing vascularized fat flaps using rabbit adipose-derived stem cells (rASCs) and collagen scaffolds in a rabbit model. We evaluated rASCs proliferation, paracrine function, adipogenesis, vascularization, and CD54 expression, with or without HIF-1α transfection in vitro and in vivo. We observed that adipogenic differentiation potential was greater in rASCs with high CD54 expression (CD54+rASCs) than in those with low expression (CD54-rASCs), both in vitro and in vivo. HIF-1α overexpression not only augmented this effect, but also enhanced cell proliferation and paracrine function in vitro. We also demonstrated that HIF-1α-transfected CD54+rASCs showed enhanced paracrine function and adipogenic capacity, and that paracrine function increases expression of angiogenesis-related markers. Thus, CD54+rASCs overexpressing HIF-1α enhanced large volume vascularized fat flap regeneration in rabbits, suggesting CD54 may be an ideal candidate marker for ASCs adipogenic differentiation.
- Published
- 2017