1. Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC
- Author
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HU Xiaorong, WANG Yize, LOU Zhiying, HU Gang, and ZHOU Guoguang
- Subjects
emulsion ,paeoniflorin ,Q1-390 ,Science (General) ,cosmetic water ,high performance liquid chromatography(hplc) ,puerarin - Abstract
A high performance liquid chromatography (HPLC) method for determination of paeoniflorin and puerarin in emulsion and cosmetic water was established. The samples were extracted with methanol by ultrasonication, and separated with acetonitrile-0.1% (mass fraction) phosphoric acid aqueous solution by gradient elution using C18 RP column and then inspected by diode array detector (DAD) detector. Under the optimum conditions,The linear relationship of the concentrations of paeoniflorin and puerarin and the intensity of UV absorptions at 232 nm and 250 nm was good in a range of 0.2-10.0 μg·mL-1,and the squared correlation coefficient (R2) was greater than 0.999. The recovery rate of two herbs was between 90.4% and 103.9%, and the relative standard deviation (RSD) was between 1.53% and 2.37%. The detection limit was between 2 mg·kg-1 and 8 mg·kg-1, meeting the requirements of the national standard for the detection limit. The developed method is suitable for the rapid, efficient, and accurate determination of paeoniflorin and puerarin in emulsion and cosmetic water.
- Published
- 2021