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BackgroundHematological medicine is a practical discipline that is difficult to study. Problem-based learning (PBL) is an innovative student-centered teaching method wherein students define their own learning objectives from clinically based problems. Considering that WeChat is the most popular communication app in China, we selected it as a new platform for online PBL to reduce the limitations of traditional PBL in hematology teaching. ObjectiveThis study aims to explore a new pedagogical method called WeChat-PBL, which is based on real micro clinical cases for postgraduates majoring in hematology and to demonstrate its feasibility and acceptability. MethodsA total of 48 hematological postgraduates and 7 tutors participated in this study. We divided the participants into 7 groups where students can learn theoretical knowledge. After each course, the members of each group were required to complete in-class quizzes. Moreover, the students and tutors were required to fill out periodic (after each class) and overall (after each semester) evaluations. ResultsA total of 8 micro clinical cases were presented in WeChat-PBL. The average quiz score for acute myelogenous leukemia, chronic myeloid leukemia, multiple myeloma, acute promyelocytic leukemia, and lymphoma were 89.0%, 86.0%, 83.4%, 88.8%, and 77.5%, respectively. Periodic evaluations showed that both students and tutors were satisfied with the process of WeChat-PBL. The overall evaluation results showed that WeChat-PBL was able to positively impact the learning experiences of hematological postgraduates. ConclusionsOur results indicate the feasibility and acceptability of the WeChat-PBL teaching method for postgraduates majoring in hematology.

AIM:To evaluate the effect of optical amplification on macular retinal thickness measurements in myopic eyes of children and adolescents using optical coherence tomography(OCT).METHODS:A total of 68 cases(126 eyes)of children and adolescents aged 6 to 18 years old attending our optometric center from April 2023 to January 2024 were selected. They were divided into 44 cases(83 eyes)in the mild myopia group(-0.50 D0.05). In the moderate myopia group, the differences between the retinal thickness in each quadrant of the macula and the mean retinal thickness before and after correction were statistically significant(all P0.05). There were significant differences between the two groups in the macular fovea, the nasal side of the inner ring, the temporal side of the inner ring, the upper inner ring, the lower inner ring, the nasal side of the outer ring, and the mean retinal thickness after correction(all P

Objective To investigate the advantages and disadvantages of purse-string suture, Hem-o-lok clip and double thread ligation in the treatment of appendiceal stump. Methods A retrospective study was conducted on 237 acute appendicitis patients who underwent laparoscopic appendectomy at Nanjing Jiangbei Hospital from July 2021 to April 2023. According to the treatment, the patients were divided into purse-string suture group (n=48), Hem-o-lok clip group (n=152) and double thread ligation group (n=37). The baseline data, surgical time, intraoperative bleeding, length of hospital stay, total hospitalization costs, and incidence of postoperative complications among three different treatment methods were compared. Results There was no significant in operation time, blood loss, and hospital stay among three groups (P＞0.05). there was significant difference in total hospitalization cost among three groups (F=5.823, P=0.003), and that in double thread ligation group was (9 809.1±1 199.7) yuan, which was significantly lower than that in the purse-string suture group ［(10 355.0±1 133.7) yuan］ and Hem-o-lok clip group ［(10 541.6±1 179.3) yuan］ (P＜0.05). The incidence of complications in the purse string suture group was 2.1% (1/48), the Hem-o-lok clip group was 2.0% (3/152), and the double thread ligation group was 2.7% (1/37). There was no statistically significant difference in the incidence of complications among the three groups (P＞0.05). Conclusion The three methods to secure the appendiceal stump are safe and effective, but double thread ligation is cost-saving than purse-string suture and Hem-o-lok clip.

Abstract Neurodegenerative diseases (NDs) are common chronic diseases arising from progressive damage to the nervous system. Here, in-house natural product database screening revealed that libertellenone C (LC) obtained from the fermentation products of Arthrinium arundinis separated from the gut of a centipede collected in our Tongji campus, showed a remarkable neuroprotective effect. Further investigation was conducted to clarify the specific mechanism. LC dose-dependently reversed glutamate-induced decreased viability, accumulated reactive oxygen species, mitochondrial membrane potential loss, and apoptosis in SH-SY5Y cells. Network pharmacology analysis predicted that the targets of LC were most likely directly related to oxidative stress and the regulation of inflammatory factor-associated signaling pathways. Further study demonstrated that LC attenuated nitrite, TNF-α, and IL-1β production and decreased inducible nitric oxide synthase and cyclooxygenase expression in lipopolysaccharide-induced BV-2 cells. LC could directly inhibit NLRP3 inflammasome activation by decreasing the expression levels of NLRP3, ASC, cleaved Caspase-1, and NF-κB p65. Our results provide a new understanding of how LC inhibits the NLRP3 inflammasome in microglia, providing neuroprotection. These findings might guide the development of effective LC-based therapeutic strategies for NDs.