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Aneuclis flavopedes Sheng, Zhou & Wei, sp. nov. (Figs 1���10) Material examined. Holotype, Female, CHINA: Aerxiang, 273 m, Zhangwu, Liaoning Province, 18 June 2020, Ya-Wei Wei (IT). Diagnosis. Head and mesosoma almost entirely leathery texture. Clypeus smooth, shiny. Malar space 1.2 �� as long as basal width of mandible. Occipital carina (Fig. 4) complete. Speculum (Fig. 6) absent. 2m-cu slightly basal of rs-m. Median longitudinal carina between anterior margin and area superomedia 0.25 �� as long as combined area of area superomedia and area petiolaris (Fig. 7). Metasomal tergites smooth, shiny. Tergite I (Figs 8, 9) about 3.4 �� as long as apical width. Subapical portion of lower valve (Fig. 10) with 3 weak edges. Tergite II red brown, lateral portions of subsequent tergites yellowish brown. Description. Holotype, female (Fig. 1). Body length approximately 2.9 mm. Fore wing length about 2.5 mm. Ovipositor sheath about 1.7 mm. Head. Inner margins of eyes almost parallel. Face (Fig. 2) slightly evenly convex, 2.3 �� as wide as long, with even leathery texture and opalescent hairs; upper median portion smooth, shiny. Clypeal sulcus vague. Clypeus 2.9 �� as wide as long, smooth, shiny, evenly convex apically; apical margin broadly slightly convex, with a fringe of parallel long brownish hairs (Fig. 2). Mandible relatively elongate, slightly narrow apically, with weak fine punctures; upper tooth slightly longer than lower tooth. Malar area shagreened. Malar space 1.2 �� as long as basal width of mandible. Gena (Figs 3, 4) entirely shagreened, strongly evenly convergent posteriorly. Vertex (Fig. 4) and frons with texture as face. Postocellar line 0.9 �� as long as ocular-ocellar line. Posterior portion of vertex evenly concave. Frons slightly evenly convex medially.Antenna with 15 flagellomeres, each one distinctly longer than its own width (Fig. 1), penultimate flagellomere about 1.5 �� as long as width. Ratios of lengths from first to fifth flagellomeres: 1.0:1.1:1.1:1.1:1.0. Occipital carina complete, distinct, reaching hypostomal carina above base of mandible. Mesosoma. Anterior margin of pronotum with dense yellowish white hairs, lateral concavity with short transverse wrinkles; dorsal posterior portion almost shiny, shagreened. Epomia indistinct. Mesoscutum (Fig. 5) wide, almost as wide as long, evenly slightly convex, with even leathery texture. Notaulus evident on anterior portion of mesoscutum. Scutoscutellar groove almost smooth. Scutellum slightly convex, with texture as mesoscutum. Posterior portion of postscutellum transversely convex, rough, indistinctly punctate. Mesopleuron and metapleuron (Fig. 6) with texture as face; speculum absent; episternal scrobe indistinct; upper anterior portion beneath subalar prominence with short indistinct transverse wrinkles. Epicnemial carina reaching to anterior margin of mesopleuron, about 0.3 �� as long as posterior margin of pronotum. Sternaulus indistinct. Metapleuron with gray white hairs. Legs relatively slender; claw simple. Ratio of length of first to fifth hind tarsomeres 4.2:1.6:1.3:1.0:1.2. Wings hyaline. Fore wing with vein 1cu-a distal of M&RS by 0.4 �� length of cu-a; section between 1cu-a and M&RS strongly widened. 2m-cu slightly basal of rs-m. 2cu-a absent. Hind wing cu 0.4 �� as long as cu-a. Propodeum (Fig. 7) evenly convex, with rough leathery texture and gray white hairs. Median longitudinal carina between basal margin and area superomedia 0.25 �� as long as combined area of area superomedia and area petiolaris. Lateral longitudinal carina between posterior transverse carina and anterior margin absent. Propodeal spiracle small, circular. Metasoma. Metasomal tergites smooth, shiny, almost without punctures. Tergite I (Figs 8, 9) about 3.4 �� as long as apical width; slender; postpetiole about 1.3 �� as long as apical width; latero-median carina absent; basal portion of dorsolateral carina present; ventro-lateral carina complete; spiracle small, circular, located at apical 0.35 of tergite I. Tergite II (Fig. 8) about 1.3 �� as long as basal width; 1.1 �� as long as apical width. Third and subsequent tergites strongly compressed. Ovipositor sheath about 2.5 �� as long as hind tibia. Ovipositor (Fig. 10) evenly curved upwards, subapical dorsal notch weak and shallow. Subapical portion of lower valve with 3 weak edges, distance between apical two about 3.0 �� as long as distance between basal two. Coloration (Fig. 1). Black, except the following. Basal-ventral portion of antenna, apical portion of clypeus, mandible except teeth, maxillary and labial palpi, tegula, legs except fifth tarsomeres, lateral portions of third and subsequent tergites yellowish brown. Second tergite red brown. Dorsal profile and apical-ventral portion of antenna brown to dark brown. Basal portion of clypeus red brown. Lower portion of pronotum irregularly reddish brown. Dorsal portions of third and subsequent tergites brownish black. Lower portion of pterostigma dark brown. Veins mostly slightly brown; M&RS, RA, 2r&RS, RS, rs-m, CU darkish brown. Distribution. CHINA: Liaoning. Etymology. The name of the new species is based on the legs being brownish yellow. Remarks. This new species is similar to A. maritimus (Thomson, 1889), but can be easily distinguished from the latter by having fore wing 2m-cu slightly basal of rs-m; clypeus reddish brown; middle and hind coxae yellowish brown. A. maritimus: 2m-cu distinctly distal of rs-m.; basal portion of clypeus black; middle and hind coxae black. The new species can be distinguished from known Oriental species by having occipital carina complete and distinct (other Oriental species with dorsal portion of occipital carina widely absent)., Published as part of Wei, Ya-Wei, Zhou, Yong-Bin & Sheng, Mao-Ling, 2021, First record of the genus Aneuclis F��rster (Hymenoptera, Ichneumonidae) from China, pp. 292-296 in Zootaxa 4908 (2) on pages 293-295, DOI: 10.11646/zootaxa.4908.2.10, http://zenodo.org/record/4438205

Aneuclis Forster, 1869 Aneuclis Forster, 1869:147. Type-species: (Isurgus rufipes Sz��pligeti) = maritimus Sz��pligeti. The genus may be distinguished from other genera by the key and figures given by Khalaim (2004, 2018). In Khalaim���s (2004) key to species, the new species can be inserted as follows (partly modified): 1. Second recurrent vein interstitial (Fig. 10) or basal of rs-m. Antenna 16���17-antennomeres............................ 2 - Second recurrent vein postfurcal (Fig. 11).................................................................. 5 2. Width of pterostigma 1.3 �� length of first radial abscissa. Ovipositor sheath 2.6 �� as long as tergite I...... 10 A. mongolica - Width of pterostigma equal to, or less than length of first radial abscissa.......................................... 3 3. Lower part of pronotum yellowish or partly reddish brown, distinctly differing in color from black mesosoma. Ovipositor sheath 2.5 �� as long as tergite I......................................................................... 3��� - Lower part of pronotum black, of same color as rest of mesosoma............................................... 4 3���. Second recurrent vein interstitial or slightly distal of rs-m. Mesopleuron usually dark brown. Metasomal tergites with brown spots........................................................................... 11. A. rufipleuris Horstm. - Second recurrent vein slightly basal of rs-m. Mesopleuron entirely black. Tergite I black, tergite II entirely reddish brown, three and subsequent tergites brownish black dorsally, reddish brown laterally........ A. flavopedes Sheng, Zhou & Wei, sp. nov., Published as part of Wei, Ya-Wei, Zhou, Yong-Bin & Sheng, Mao-Ling, 2021, First record of the genus Aneuclis F��rster (Hymenoptera, Ichneumonidae) from China, pp. 292-296 in Zootaxa 4908 (2) on pages 292-293, DOI: 10.11646/zootaxa.4908.2.10, http://zenodo.org/record/4438205, {"references":["Forster, A. (1869) Synopsis der Familien und Gattungen der Ichneumonen. Verhandlungen des Naturhistorischen Vereins der Preussischen Rheinlande und Westfalens, 25 (1868), 135 - 221.","Khalaim, A. I. (2004) A review of the genera Aneuclis Forster and Sathropterus Forster (Hymenoptera, Ichneumonidae, Tersilochinae). Entomological Review, 84 (8), 922 - 934.","Khalaim, A. I. (2018) The genera Allophrys Forster and Aneuclis Forster (Hymenoptera: Ichneumonidae: Tersilochinae) of Vietnam. Zootaxa, 4378 (3), 414 - 428. https: // doi. org / 10.11646 / zootaxa. 4378.3.9"]}

For disinfection of Arabidopsis thaliana seeds, the seeds of WT and EX (independent transgenic lines 4, 5 and 6) lines was disinfected with sodium hypochlorite. After 3 days of vernalization at 4 °C, the plates containing the seeds was transferred to a growth chamber. For statistical methods of data, the data shown are the means ± SDs (n = 80) of three experiments. ANOVA tests demonstrated that there were significant differences (∗ P < 0.05, ∗∗ P < 0.01). Expression of AtActin was analyzed as a loading control in Arabidopsis (Table S1). Expression of GmActin was analyzed as a control in soybean (Table S1.) Figure S1. Quantitative gene expression of all MYB-related TF family members in soybean. The tissue expression data of quantified prediction for a diverse set of fourteen tissue types from the soybase website ( http://soybase.org/soyseq/ ). Figure S2. Subcellular localization and expression level of GmMYB118. GmMYB118 was localized in the nucleus in Arabidopsis mesophyll protoplasts (A). Scale bars = 20 μm. GmMYB118 gene expression in ectopic expression and WT (Col-0) plants (B). Figure S3. Germination rate of OE lines under PEG treatment. Images of germinating EX and WT (Col-0) seeds after 72 h under 0, 3, 6 and 9% PEG6000 treatment (A). The germination of the WT and EX plants of sown on 1/2-strength MS growth media with 0% (B), 3% (C), 6% (D) and 9% PEG6000 (E) were monitored until to 72 h. Figure S4. Germination rate of EX lines under NaCl treatment. Images of germinating EX and WT (Col-0) seeds after 72 h under 0, 75, 100 and 125 mM NaCl treatment (A). The germination of the WT and EX plants of sown on 1/2-strength MS growth media with 0 (B), 75 (C), 100 (D) and 125 mM NaCl (E) were monitored until to 72 h. Figure S5. Targeted mutagenesis in soybean hairy roots and GUS staining. The GUS staining of transgenic hairy roots revealed a transformation efficiency of approximately 50% (A). The target gene was amplified with specific primers and sequenced (AuGCT, China). The results show that some bases have been replaced (B). Figure S6. Expression level of GmMYB118 under drought and salt treatments. Expression level of GmMYB118 under drought (B) and salt (C) treatments was quantified by qRT-PCR assays. The expression level of GmMYB118 under normal condition was shown in Figure S6A. Table S1. Gene-specific primers used for qRT-PCR. Table S2. Primers used to construct recombinant vectors. (PDF 753 kb)