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Your search keyword '"Zhu, Laikuan"' showing total 21 results
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21 results on '"Zhu, Laikuan"'

12. Additional file 1 of Highly sensitive and portable mRNA detection platform for early cancer detection

Author

Li, Hongxia, Warden, Antony R., Su, Wenqiong, He, Jie, Zhi, Xiao, Wang, Kan, Zhu, Laikuan, Shen, Guangxia, and Ding, Xianting

Abstract

Additional file 1: Table S1. Sequences of all probes in experiments. Figure S1. Simulation of all structures produced in CHA process. Figure S2. The stability ofgold signal probes in high salt buffer. FigureS3. UV–VIS of raw gold nanoparticles and gold signal probes. Figure S4. UV–VIS of gold signal probes before and after amplification. Figure S5. The gold nanoparticles wavelength various with time in different enhancement buffer. Figure S6. The goldnanoparticles wavelength various with time in different enhancement buffer. Figure S7. The gold nanoparticles grayscale various with time in different enhancement buffer. Figure S8. The appearance of enhancement buffer treatment in strips. Figure S9. Specificity of CHAGE stripes for detection of GPC1 mRNA. Figure S10. The stability of gold signal probes in buffer and strips. Table S2. Comparison of different detection methods.

Published
2021
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20. Differential proteomics analysis on fluoride-resistant Streptococcus mutansby label-free quantitation

Author

Zhao, Hongyan, Zhang, Zhimin, Zhu, Laikuan, Li, Zhenling, Li, Penglian, Liu, Jiabai, and Zhang, Zhiying

Abstract

To figure out the changed biological characteristics of a fluoride-resistant strain compared with those of the parent sensitive strain of Streptococcus mutans(S. mutans) with the help of differential proteomics analysis, sixty-seven proteins with obvious differential expression were identified in fluoride-resistant strain. Among them are the important virulence factors causing caries, including glucosyltransferase-S, glucosyltransferase-I, glucosyltransferase-Si, major cell-surface adhesin Pac, F-ATPase β-subunit, Ffh, molecular chaperone DnaK, chaperonin GroEL, and the lactate dehydrogenase. We obtained the differential proteomics expression profiles of the fluoride-resistant strain(UA159-FR) and its parental strain(UA159) of S. mutans. Several important virulence factors bringing about caries were identified to have differential expressions, among which the expression of lactate dehydrogenase in fluoride-resistant strain was enhanced compared to that in its parent strain. The results could provide the basis for analysing the functions of proteins encoded by caries-related genes from the fluoride-resistant S. mutansstrain.

Published
2014
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21. Crystallization and preliminary X-ray analysis of S-ribosylhomocysteinase from Streptococcus mutans.

Author

Li H, Zhao H, Zhu L, Hong L, Zhang H, Lin F, Xu C, Li S, and Zhang Z

Subjects
Crystallization, Crystallography, X-Ray, Bacterial Proteins chemistry, Carbon-Sulfur Lyases chemistry, Streptococcus mutans enzymology
Abstract

S-Ribosylhomocysteinase (LuxS) encoded by the luxS gene from Streptococcus mutans plays a crucial role in the quorum-sensing system. LuxS was solubly expressed in Escherichia coli with high yield. The purity of the purified target protein, which was identified by SDS-PAGE and MALDI-TOF MS analysis, was >95%. The protein was crystallized using the hanging-drop vapour-diffusion method with PEG 3350 as the primary precipitant. X-ray diffraction data were collected at Beijing Synchrotron Radiation Facility (BSRF). Diffraction by the crystal extended to 2.4 Å resolution and the crystal belonged to space group C222(1), with unit-cell parameters a = 55.3, b = 148.7, c = 82.8 Å.

Published
2012
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