Search

To investigate the influence of the number of inlet pipes on the characteristics of flow field and the discharge of solid particles such as bait residue and fish feces, STAR-CCM + was used to analyze the flow of field distribution in a ship-based aquaculture cabin under the conditions of two and four inlet pipes, thus optimizing the inlet pipes of the ship-based aquaculture cabin. Furthermore, a three-phase flow model (solid–liquid–gas) was established to determine the movement characteristics of similar solid particles of residual bait and fish manure in the cabin, while the emission performance of solid particles was analyzed. Finally, the formation of solid particles in the center of the aquaculture cabin was simulated. The simulation results show that the effect of flow field is better with two inlet pipes, and the discharge volume of the bottom outlet has little impact on the emission of solid particles. Findings from this study can serve as a reference and basis for the design and optimization of the aquaculture cabin of an aquaculture vessel. [ABSTRACT FROM AUTHOR]

Bergenin is a C‐glucoside of 4‐O‐methyl gallic acid with a variety of biological activities, such as antioxidant and anti‐inflammatory. Herein, we investigated the involvement of bergenin in the protective effect against H2O2‐induced oxidative stress and apoptosis in human nucleus pulposus cells (HNPCs) and the underlying mechanisms. HNPCs were cotreated with various concentrations of bergenin and 200 μM H2O2 for 24 h. Cell viability was detected by Cell Counting Kit‐8 and lactate dehydrogenase release assays. Reactive oxygen species (ROS) was evaluated utilizing 2′,7′‐dichlorofluorescein‐diacetate. Superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) levels were measured to assess oxidative stress. Apoptosis was evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling and caspase‐3/7 activity assays. Expression of protein was determined by western blotting. Results indicated that treatment with bergenin significantly alleviated H2O2‐induced viability reduction and ROS overproduction in HNPCs in a dose‐dependent manner. Bergenin alleviated H2O2‐induced oxidative stress in HNPCs by increased activity of superoxide dismutase and level of glutathione peroxidase. H2O2‐induced apoptosis and activity of caspase‐3/7 were also suppressed by bergenin treatment in HNPCs. Western blotting showed that H2O2‐induced decrease in expression of peroxisome proliferator‐activated receptor γ (PPAR‐γ) and increase in nuclear factor κB (NF‐κB) were inhibited by bergenin. However, the inhibitory effect of bergenin on H2O2‐induced viability reduction, oxidative stress and apoptosis were noticeably abrogated in PPAR‐γ knockdown HNPCs. In conclusion, our results indicated that bergenin alleviates H2O2‐induced oxidative stress and apoptosis in HNPCs by activating PPAR‐γ and suppressing NF‐κB pathway. [ABSTRACT FROM AUTHOR]

Bergenin is a C-glucoside of 4-O-methyl gallic acid with a variety of biological activities, such as antioxidant and anti-inflammatory. Herein, we investigated the involvement of bergenin in the protective effect against H 2 O 2 -induced oxidative stress and apoptosis in human nucleus pulposus cells (HNPCs) and the underlying mechanisms. HNPCs were cotreated with various concentrations of bergenin and 200 μM H 2 O 2 for 24 h. Cell viability was detected by Cell Counting Kit-8 and lactate dehydrogenase release assays. Reactive oxygen species (ROS) was evaluated utilizing 2',7'-dichlorofluorescein-diacetate. Superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) levels were measured to assess oxidative stress. Apoptosis was evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling and caspase-3/7 activity assays. Expression of protein was determined by western blotting. Results indicated that treatment with bergenin significantly alleviated H 2 O 2 -induced viability reduction and ROS overproduction in HNPCs in a dose-dependent manner. Bergenin alleviated H 2 O 2 -induced oxidative stress in HNPCs by increased activity of superoxide dismutase and level of glutathione peroxidase. H 2 O 2 -induced apoptosis and activity of caspase-3/7 were also suppressed by bergenin treatment in HNPCs. Western blotting showed that H 2 O 2 -induced decrease in expression of peroxisome proliferator-activated receptor γ (PPAR-γ) and increase in nuclear factor κB (NF-κB) were inhibited by bergenin. However, the inhibitory effect of bergenin on H 2 O 2 -induced viability reduction, oxidative stress and apoptosis were noticeably abrogated in PPAR-γ knockdown HNPCs. In conclusion, our results indicated that bergenin alleviates H 2 O 2 -induced oxidative stress and apoptosis in HNPCs by activating PPAR-γ and suppressing NF-κB pathway., (© 2021 Wiley Periodicals LLC.)