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361 results on '"Zhu, Yefei"'

14. Metagenomic and metabolomic remodeling in nonagenarians and centenarians and its association with genetic and socioeconomic factors

Author

Xu, Qian, Wu, Chunyan, Zhu, Qi, Gao, Renyuan, Lu, Jianquan, Valles-Colomer, Mireia, Zhu, Jian, Yin, Fang, Huang, Linsheng, Ding, Lulu, Zhang, Xiaohui, Zhang, Yonghui, Xiong, Xiao, Bi, Miaomiao, Chen, Xiang, Zhu, Yefei, Liu, Lin, Liu, Yongqiang, Chen, Yongshen, Fan, Jian, Sun, Yan, Wang, Jun, Cao, Zhan, Fan, Chunsun, Ehrlich, S. Dusko, Segata, Nicola, Qin, Nan, and Qin, Huanlong

Published
2022
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29. Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.

Author

Zhang, Guiyuan, Ding, Yajie, Zhang, Hao, Wei, Dong, Liu, Yufeng, Sun, Jie, Xie, Zhuoying, Tao, W. Andy, and Zhu, Yefei

Subjects
SAMPLING (Process), PROTEOMICS, EXTRACELLULAR vesicles, VESICLES (Cytology), PROSTATE diseases, URINE, WESTERN immunoblotting
Abstract

Extracellular vesicles (EVs) in urine are a promising source for developing non-invasive biomarkers. However, urine concentration and content are highly variable and dynamic, and actual urine collection and handling often is nonideal. Furthermore, patients such as those with prostate diseases have challenges in sample collection due to difficulties in holding urine at designated time points. Here, we simulated the actual situation of clinical sample collection to examine the stability of EVs in urine under different circumstances, including urine collection time and temporary storage temperature, as well as daily urine sampling under different diet conditions. EVs were isolated using functionalized EVtrap magnetic beads and characterized by nanoparticle tracking analysis (NTA), western blotting, electron microscopy, and mass spectrometry (MS). EVs in urine remained relatively stable during temporary storage for 6 hours at room temperature and for 12 hours at 4 °C, while significant fluctuations were observed in EV amounts from urine samples collected at different time points from the same individuals, especially under certain diets. Sample normalization with creatinine reduced the coefficient of variation (CV) values among EV samples from 17% to approximately 6% and facilitated downstream MS analyses. Finally, based on the results, we applied them to evaluate potential biomarker panels in prostate cancer by data-independent acquisition (DIA) MS, presenting the recommendation that can facilitate biomarker discovery with nonideal handling conditions. [ABSTRACT FROM AUTHOR]

Published
2024
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34. Author Correction: Metagenomic and metabolomic remodeling in nonagenarians and centenarians and its association with genetic and socioeconomic factors

Author

Xu, Qian, Wu, Chunyan, Zhu, Qi, Gao, Renyuan, Lu, Jianquan, Valles-Colomer, Mireia, Zhu, Jian, Yin, Fang, Huang, Linsheng, Ding, Lulu, Zhang, Xiaohui, Zhang, Yonghui, Xiong, Xiao, Bi, Miaomiao, Chen, Xiang, Zhu, Yefei, Liu, Lin, Liu, Yongqiang, Chen, Yongshen, Fan, Jian, Sun, Yan, Wang, Jun, Cao, Zhan, Fan, Chunsun, Ehrlich, S. Dusko, Segata, Nicola, Qin, Nan, and Qin, Huanlong

Published
2022
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41. Table S4 from Fusobacterium Nucleatum Promotes the Development of Colorectal Cancer by Activating a Cytochrome P450/Epoxyoctadecenoic Acid Axis via TLR4/Keap1/NRF2 Signaling

Author

Kong, Cheng, primary, Yan, Xuebing, primary, Zhu, Yefei, primary, Zhu, Huiyuan, primary, Luo, Ying, primary, Liu, Peipei, primary, Ferrandon, Sylvain, primary, Kalady, Matthew F., primary, Gao, Renyuan, primary, He, Jide, primary, Yin, Fang, primary, Qu, Xiao, primary, Zheng, Jiayi, primary, Gao, Yaohui, primary, Wei, Qing, primary, Ma, Yanlei, primary, Liu, Jun-Yan, primary, and Qin, Huanlong, primary

Published
2023
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42. Data from Fusobacterium Nucleatum Promotes the Development of Colorectal Cancer by Activating a Cytochrome P450/Epoxyoctadecenoic Acid Axis via TLR4/Keap1/NRF2 Signaling

Author

Kong, Cheng, primary, Yan, Xuebing, primary, Zhu, Yefei, primary, Zhu, Huiyuan, primary, Luo, Ying, primary, Liu, Peipei, primary, Ferrandon, Sylvain, primary, Kalady, Matthew F., primary, Gao, Renyuan, primary, He, Jide, primary, Yin, Fang, primary, Qu, Xiao, primary, Zheng, Jiayi, primary, Gao, Yaohui, primary, Wei, Qing, primary, Ma, Yanlei, primary, Liu, Jun-Yan, primary, and Qin, Huanlong, primary

Published
2023
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43. Supplementary Materials from Fusobacterium Nucleatum Promotes the Development of Colorectal Cancer by Activating a Cytochrome P450/Epoxyoctadecenoic Acid Axis via TLR4/Keap1/NRF2 Signaling

Author

Kong, Cheng, primary, Yan, Xuebing, primary, Zhu, Yefei, primary, Zhu, Huiyuan, primary, Luo, Ying, primary, Liu, Peipei, primary, Ferrandon, Sylvain, primary, Kalady, Matthew F., primary, Gao, Renyuan, primary, He, Jide, primary, Yin, Fang, primary, Qu, Xiao, primary, Zheng, Jiayi, primary, Gao, Yaohui, primary, Wei, Qing, primary, Ma, Yanlei, primary, Liu, Jun-Yan, primary, and Qin, Huanlong, primary

Published
2023
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