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With the increasingly strict control of hazardous waste in enterprises by the state, the issues regarding storage, transfer and compliance disposal of hazardous waste in small and micro enterprises are increasingly prominent, and their research get more and more attention. The management of small and micro waste enterprises with "many but scattered" has become an important part of ecological environment protection. This paper takes 358 papers related to the hazardous waste management of small and micro enterprises included in the CNKI database from 2004 to 2022 as the research object, conducts bibliometric analysis by CiteSpace and VOSviewer softwares, composes the research status of related papers by using trend analysis of issuance, analysis of major research institutions and research scholars, and further analyzes the current research hotspots by keyword co-occurrence. [ABSTRACT FROM AUTHOR]

In the development of dendritic cell (DC) vaccines, the maturation of DCs is a critical stage. Adjuvants play a pivotal role in the maturation of DCs, with a major concern being to ensure both efficacy and safety. This study introduces an innovative approach that combines high efficacy with safety through the synthesis of micro-adjuvants grafted with copolymers of 2-(methacrylamido) glucopyranose (MAG) and methacryloxyethyl trimethyl ammonium chloride (DMC). The utilization of metal-free surface-initiated atom transfer radical polymerization enables the production of safe and recyclable adjuvants. These micrometer-sized adjuvants surpass the optimal size range for cellular endocytosis, enabling the retrieval and reuse of them during the ex vivo maturation process, mitigating potential toxicity concerns associated with the endocytosis of non-metabolized nanoparticles. Additionally, the adjuvants exhibit a "micro-ligand-mediated maturation enhancement" effect for DC maturation. This effect is influenced by the shape of the particle, as evidenced by the distinct promotion effects of rod-like and spherical micro-adjuvants with comparable sizes. Furthermore, the porous structure of the adjuvants enables them to function as cargo-carrying "micro-shuttles", releasing antigens upon binding to DCs to facilitate efficient antigen delivery., (© 2024 Wiley-VCH GmbH.)

Introduction: The raise of multi-drug resistant bacteria involving carbapenem, colistin, or tigecycline resistance constitutes a threat to public health, which partly results from the transmission of corresponding mobile resistance genes, such as bla KPC and bla NDM for carbapenem, mcr for colistin, and tmexCD-toprJ gene cluster for tigecycline. Herein, we described the emergence of an Aeromonas veronii strain HD6454 co-harboring bla KPC-2 , mcr-3.17 , and tmexC3.2-tmexD3.3-toprJ1b gene cluster from hospital sewage., Methods: Whole genome sequencing (WGS) was used to determine the genome sequence of HD6454, and the detailed genomic analysis of genetic elements or regions carrying key antimicrobial resistance genes (ARGs) from HD6454 were performed. Cloning experiment was conducted to confirm the function of key ARGs in mediating antimicrobial resistance. Conjugation experiment was conducted to determine the mobility of the plasmid., Results: The results showed that this strain belonged to a novel sequence type (ST) variant ST1016, and carried 18 important ARGs. Among them, the bla KPC-2 was carried by non-self-transmissible IncP-6 plasmid, while tmexC3.2-tmexD3.3-toprJ1b gene cluster and mcr-3.17 were carried by integrative and mobilizable element (IME) or IME-related region in chromosome. The mcr-3.17 , mcr-3.6 , and mcr-3-like3 genes were further inferred to originate from IMEs of Aeromonas species. Additionally, for the first time, the mcr-3.17 was confirmed to confer low-level resistance to colistin under inducible expression, while tmexC3.2-tmexD3.3-toprJ1b gene cluster was confirmed to confer low-level resistance to tigecycline., Discussion: This is the first report of a strain co-harboring bla KPC-2 , mcr-3.17 , and tmexC3.2-tmexD3.3-toprJ1b gene cluster. Although the resistance and/or mobility of these ARGs are limited in this strain, the emergence of this multiple important ARGs-carrying strain deserves further attention., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Zhu, Wu, Zhu, Wang, Wen, Yang, Lv, Zhang, Chen and Du.)

The quinolone resistance crpP genes can mediate decreased susceptibility to quinolones. However, diversification and prevalence of crpP genes and crpP -carrying integrative and conjugative elements (ICEs) still need to be elucidated. In this study, genome sequencing was conducted for 200 Chinese Pseudomonas aeruginosa isolates, 16 of which were fully sequenced. All the 37 available CrpP variants were collected for phylogenetic analysis, 10 CrpP enzymes were chosen to conduct cloning and antimicrobial susceptibility test, and 22 crpP -carrying Tn 6786 -related ICEs were selected for detail genetic dissection analysis. Then, typing/nomenclature schemes for crpP variants and crpP -carrying ICEs were established for the first time. The 10 representative CrpP enzymes were confirmed to mediate decreased susceptibility to one to three quinolones. Tn 6786 -related ICEs displayed high-level diversification in both nucleotide sequences and modular structures. Mainly, massive gene acquisition/loss occurred across the whole genomes of Tn 6786 -related ICEs. 53.5% (107/200) of the tested clinical P. aeruginosa isolates from China carried crpP genes, which were exclusively located within chromosome-borne Tn 6786 -related ICEs. The crpP -carrying ICEs were at active stages of evolution and had the high potential to be an important vector for the dissemination of resistance genes besides crpP . The present study furthered the understanding of the bioinformatics and epidemiology of crpP genes and crpP -carrying ICEs.

We report on assembly and stimuli-response behavior of layer-by-layer (LbL) films of pH- and temperature-responsive cationic diblock copolymer micelles (BCMs) of poly(2-(dimethylamino)ethyl methacrylate)-block-poly(N-isopropylacrylamide) (PDMA-b-PNIPAM) and a linear polyanion polystyrene sulfonate (PSS). As a function of solution pH at temperatures above lower critical solution temperature (LCST) of PNIPAM, PDMA-b-PNIPAM micelles have been demonstrated earlier to exhibit an abrupt change in micellar aggregation number and hydrodynamic size between larger and smaller BCMs (LBCMs and SBCMs, respectively). Here, LBCMs or SBCMs were included within LbL films through self-assembly with a polyanion, and film pH and temperature responses were studied using ellipsometry and atomic force microscopy (AFM). Both types of micelle preserved their micellar morphology when adsorbed at the surface of oxidized silicon wafers coated with PSS-terminated precursor layer at a constant pH. Response of adsorbed BCMs to temperature and pH variations was strongly dependent on whether or not BCMs were coated with the PSS layer. While monolayers of LBCMs lost their original dry morphology in response to pH or temperature variations, depositing a PSS layer atop LBCMs inhibited such irreversible restructuring. As a result of wrapping around and strong binding of PSS chains with LBCM micelles, BCM/PSS assemblies preserved their original dry state morphology despite the application of pH and temperature triggers. However, the wet-state film response to pH and temperature stimuli was drastically different. Swelling of BCM/PSS multilayers was strongly affected by temperature but was almost independent of pH due to neutralization of BCM PDMA's coronal charge with PSS. Cycling the temperature below and above PNIPAM's LCST caused PNIPAM chains within BCM cores to swell or collapse, resulting in reversible swelling transitions in the entire BCM/PSS assemblies. Temperature-controlled switching between the hydrophobic and hydrophilic state of assembled micellar cores was also used to regulate the release of a micelle-loaded hydrophobic pyrene dye, whose release rate increased at temperatures below PNIPAM's LCST.

In the mol-ecule of the title compound, C(28)H(40)N(2)O(2), the tert-butyl group is disordered over two positions; site-occupation factors were kept fixed at 0.5. The morpholine ring has a chair conformation. Intra-molecular O-H⋯N hydrogen bonding results in the formation of a planar six-membered ring, which is oriented at a dihedral angle of 0.70 (3)° with respect to the adjacent aromatic ring. The dihedral angle between the benzene rings is 67.66 (3)°.