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Additive manufacturing (AM) technologies have advanced from rapid prototyping to becoming viable manufacturing solutions, offering users both design flexibility and mechanical properties that meet ISO/ASTM standards. Powder bed fusion using a laser beam (PBF-LB), a popular additive manufacturing process (aka 3D printing), is used for the cost-effective production of high-quality products for the medical, aviation, and automotive industries. Despite the growing variety of metallic powder materials available for the PBF-LB process, there is still a need for new materials and procedures to optimize the processing parameters before implementing them into the production stage. In this study, we explored the use of a checkerboard scanning strategy to create samples of various sizes (ranging from 130 mm3 to 8000 mm3 using parameters developed for a small 125 mm3 piece). During the PBF-LB process, all samples were fabricated using Ti grade 2 and were in situ alloyed with a precisely controlled amount of oxygen (0.1–0.4% vol.) to enhance their mechanical properties using a solid solution strengthening mechanism. The samples were fabricated in three sets: I. Different sizes and orientations, II. Different scanning strategies, and III. Rods for high-cycle fatigue (HCF). For the tensile tests, micro samples were cut using WEDM, while for the HCF tests, samples were machined to eliminate the influence of surface roughness on their mechanical performance. The amount of oxygen in the fabricated samples was at least 50% higher than in raw Ti grade 2 powder. The O2-enriched Ti produced in the PBF-LB process exhibited a tensile strength ranging from 399 ± 25 MPa to 752 ± 14 MPa, with outcomes varying based on the size of the object and the laser scanning strategy employed. The fatigue strength of PBF-LB fabricated Ti was 386 MPa, whereas the reference Ti grade 2 rod samples exhibited a fatigue strength of 312 MPa. Our study revealed that PBF-LB parameters optimized for small samples could be adapted to fabricate larger samples using checkerboard ("island") scanning strategies. However, some additional process parameter changes are needed to reduce porosity. [ABSTRACT FROM AUTHOR]