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A remote monitoring system with the intelligent compaction index CMV as the core is designed and developed to address the shortcomings of traditional subgrade compaction quality evaluation methods. Based on the actual project, the correlation between the CMV and conventional compaction indexes of compaction degree K and dynamic resilient modulus E is investigated by applying the one-dimensional linear regression equation for three types of subgrade fillers, clayey gravel, pulverized gravel, and soil-rock mixed fill, and the scheme of fitting CMV to the mean value of conventional indexes is adopted, which is compared with the scheme of fitting CMV to the single point of conventional indexes in the existing specification. The test results show that the correlation between the CMV and conventional indexes of clayey gravel and pulverized gravel is much stronger than that of soil-rock mixed subgrades, and the correlation coefficient can be significantly improved by fitting CMV to the mean of conventional indexes compared with single-point fitting, which can be considered as a new method for intelligent rolling correlation verification.

Neuroblastoma is a heterogeneous embryonal malignancy and the most deadly tumor of childhood, although a minor subset may show spontaneous differentiation. It arises from the multipotent neural crest lineage during development. Some of this multipotency is retained in neuroblastoma, which can give rise to both adrenergic and mesenchymal tumor cells. The mechanisms enabling such dual fates are unknown, but likely help neuroblastoma to evade existing therapies. To understand neuroblastoma plasticity, we analyzed patient tumors using single-cell transcriptomics. In addition to the heterogeneous adrenergic and mesenchymal populations, we identify a subpopulation of malignant cells resembling Schwann cell precursors (SCPs). This SCP-like population connects the adrenergic and mesenchymal compartments through transitions structurally reminiscent of the SCP cell-fate decision fork that occurs during normal development. While the directionality of such transitions in neuroblastoma remains to be established, this finding expands the potential reservoirs of malignant cells, and suggests intratumoral plasticity mechanisms relevant for therapeutic resistance and relapse.

Most genome-wide association and fine-mapping studies to date have been conducted in individuals of European descent, and genetic studies of populations of Hispanic/Latino and African ancestry are limited. In addition, these populations have more complex linkage disequilibrium structure. In order to better define the genetic architecture of these understudied populations, we leveraged >100,000 phased sequences available from deep-coverage whole genome sequencing through the multi-ethnic NHLBI Trans-Omics for Precision Medicine (TOPMed) program to impute genotypes into admixed African and Hispanic/Latino samples with genome-wide genotyping array data. We demonstrated that using TOPMed sequencing data as the imputation reference panel improves genotype imputation quality in these populations, which subsequently enhanced gene-mapping power for complex traits. For rare variants with minor allele frequency (MAF) < 0.5%, we observed a 2.3- to 6.1-fold increase in the number of well-imputed variants, with 11–34% improvement in average imputation quality, compared to the state-of-the-art 1000 Genomes Project Phase 3 and Haplotype Reference Consortium reference panels. Impressively, even for extremely rare variants with minor allele count 86%. Subsequent association analyses of TOPMed reference panel-imputed genotype data with hematological traits (hemoglobin (HGB), hematocrit (HCT), and white blood cell count (WBC)) in ~21,600 African-ancestry and ~21,700 Hispanic/Latino individuals identified associations with two rare variants in the HBB gene (rs33930165 with higher WBC [p = 8.8x10-15] in African populations, rs11549407 with lower HGB [p = 1.5x10-12] and HCT [p = 8.8x10-10] in Hispanics/Latinos). By comparison, neither variant would have been genome-wide significant if either 1000 Genomes Project Phase 3 or Haplotype Reference Consortium reference panels had been used for imputation. Our findings highlight the utility of the TOPMed imputation reference panel for identification of novel rare variant associations not previously detected in similarly sized genome-wide studies of under-represented African and Hispanic/Latino populations., Author summary Admixed African and Hispanic/Latino populations remain understudied in genetic studies of complex diseases. These populations have more complex linkage disequilibrium (LD) structure that can impair mapping of variants. Genotype imputation represents an approach to improve genome coverage, especially for rare or ancestry-specific variation; however, these understudied populations also have smaller relevant imputation reference panels. In this study, we leveraged >100,000 phased sequences generated from the multi-ethnic NHLBI TOPMed project for imputation in ~21,600 individuals of African ancestry (AAs) and ~21,700 Hispanics/Latinos. We demonstrated substantially higher imputation quality for low frequency and rare variants in comparison to the 1000 Genomes Project and Haplotype Reference Consortium reference panels. Analysis of quantitative hematological traits led to the discovery of associations with two rare variants in the HBB gene; one of these variants was replicated in an independent sample, and the other is known to cause anemia in the homozygous state. By comparison, the same HBB variants would not have been genome-wide significant using current reference panels due to lower imputation quality. Our findings demonstrate the power of TOPMed whole genome sequencing data for imputation and subsequent association analysis in admixed African and Hispanic/Latino populations.

To explore the hotspots in the area of Smart education in China, 245 related articles form the China National Knowledge Infrastructure (CKNI) were analyzed by the visualization software of CiteSpace. The results show that: (1) the studies of smart education began in 2003 while there were sporadic papers published in the next ten years. There was an increasing trend year by year from 2013 to 2017 and the number of articles reached the historical peak in 2017 with 52 articles. Xianming Yang, Jiangsu Normal University and E-Education Research topped the lists of contributing authors, organizations and publications respectively. (2) “smart education”, “educational informationization”, “intelligent education” and “smart campus” were the top four largest clusters in the domain of smart education in China.

Ceramsite concrete is a light weight and high strength material. The influence of every factor to the strength of ceramsite concrete in different age was discussed by way of orthogonal experiment. It was shown that the ratio of water to bender is a key factor affecting compressive and flexural strength of ceramsite concrete. Ceramsite concrete strength increases with the bender materials agent. The earlier period strength of ceramsite concrete would decrease whereas the later period strength would increase with flyash being added. There is little affecting that ceramsite grain volume to earlier but a certain degrees to later period strength of ceramsite concrete. Ceramsite concrete may be used in bridge decks. The Shanmenkou Bridge deck is performing well after being reconstructed with ceramsite concrete a year and a half. ago There are no crack and scaling failures in the bridge. Great economic and social profits to use ceramsite concrete in bridge decks can be found.

An index system of synthetically valuation for highway construction project must show its aim behavior. In term of aim dividing method, the index system of synthetically valuation for highway construction project was shown in 3 one degree indices: the economic index (J), social index (S), and environment index (H). Every next administrative level of index has an exact valuation rule index. In a synthetically valuation index system, an influence factor of highway construction project was divided to some subsystem. Based on fuzzy synthetically valuation, a synthetically valuation index system and value were established using multi-hierarchy fuzzy valuation model. When to valuate an enterprise synthetically, decision maker would widen the composition of fuzzy operator among several valuation mathematical models in which the construction project may be fuzzy valuated. The valuation principle is as follows: every factors are all thought over, important factors are thought over only, important factors are thought over emphatically, and all are thought over as well as important factors are paid attention. A detailed evaluating method of weight coefficients can be gotten using dynamic theory. A reliable and viable method was put out for synthetically valuation of highway construction project.