1. Interferon-α suppresses invasion and enhances cisplatin-mediated apoptosis and autophagy in human osteosarcoma cells
- Author
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Zongshen Yin, Ming-Li Wang, Jing-xian Chen, Jun Chang, Dong-Mei Gao, Zeng Li, Shi-ping Wang, and Jun Zhao
- Subjects
Cancer Research ,autophagy ,medicine.medical_treatment ,cisplatin ,Biology ,Flow cytometry ,chemistry.chemical_compound ,interferon-α ,Interferon ,medicine ,Cisplatin ,medicine.diagnostic_test ,Matrigel Invasion Assay ,Acridine orange ,apoptosis ,Articles ,medicine.disease ,Cytokine ,Oncology ,chemistry ,Apoptosis ,osteosarcoma cell ,Immunology ,Cancer research ,Osteosarcoma ,medicine.drug - Abstract
Interferon (IFN)-α is generated in response to viral infections and is used clinically in the therapy of a variety of viral infections and cancers. The present study investigated whether IFN-α could inhibit the invasive ability of osteosarcoma cells using a Matrigel invasion assay. In addition, the osteosarcoma cells were treated with cisplatin and/or IFN-α. Apoptosis and autophagy were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Hoechst 33258 staining, flow cytometry assay, acridine orange staining, green fluorescent protein-LC3 dot assay and transmission electron microscopy. Further analysis revealed that the efficacy of cisplatin was enhanced by the addition of the cytokine, IFN-α. These results indicate that the combination therapy of chemotherapeutics and IFN-α is a new approach for osteosarcoma, which requires validation by experiments in vivo.
- Published
- 2013