1. Urine metabolomics of rats with chronic atrophic gastritis.
- Author
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Zu, Guo-Xiu, Sun, Qian-Qian, Chen, Jian, Liu, Xi-Jian, Sun, Ke-Yun, Zhang, Liang-Kun, Li, Ling, Han, Tao, and Huang, Hai-Liang
- Subjects
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ATROPHIC gastritis , *METABOLOMICS , *METABOLITES , *LIQUID chromatography-mass spectrometry , *URINE , *RATS - Abstract
Background/aim: To use liquid chromatography-mass spectrometry (LC-MS) to identify endogenous differential metabolites in the urine of rats with chronic atrophic gastritis (CAG). Materials and methods: Methylnitronitrosoguanidine (MNNG) was used to produce a CAG model in Wistar rats, and HE staining was used to determine the pathological model. LC-MS was used to detect the differential metabolic profiles in rat urine. Diversified analysis was performed by the statistical method. Results: Compared with the control group, the model group had 68 differential metabolites, 25 that were upregulated and 43 that were downregulated. The main metabolic pathways were D-glutamine and D-glutamic acid metabolism, histidine metabolism and purine metabolism. Conclusion: By searching for differential metabolites and metabolic pathways in the urine of CAG rats, this study provides effective experimental data for the pathogenesis and clinical diagnosis of CAG. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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