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9. Design and Synthesis of 2'-Functionalised Oligonucleotides. Their Application for Covalent Trapping the Protein - DNA Complexes

Author

Dolinnaya, Nina G., Zubin, Eugeny M., Kubareva, Elena A., Zatsepin, Timofey S., and Oretskaya, Tatiana S.

Abstract

This review outlines the design and synthesis of oligonucleotides bearing 2'-modified residues at predetermined positions within the strand. The relative merits of incorporation of reactive carboxyl, carbonyl, iodoacetamide and disulfide-containing groups into oligonucleotides were considered along with solid-phase synthesis of DNA 2'- conjugates. The specific cross-linking of 2'-substituted oligonucleotides to nucleic acid-binding proteins (transcription factor NF-κB, restriction-modification enzymes) was shown to be helpful in scanning the protein-DNA interface and studying the conformational dynamics of biopolymer ensembles. The future perspectives of chemically reactive DNA constructs as specific protein decoys are discussed.

Published
2009

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