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Your search keyword '"Zulfiya Cernochova"' showing total 6 results
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6 results on '"Zulfiya Cernochova"'

1. Thermoresponsive triblock copolymers as widely applicable 19F magnetic resonance imaging tracers

Author

Kristyna Kolouchova, Ondrej Groborz, Miroslav Slouf, Vit Herynek, Laurens Parmentier, David Babuka, Zulfiya Cernochova, Filip Koucky, Ondrej Sedlacek, Martin Hruby, Richard Hoogenboom, and Sandra Van Vlierberghe

Subjects
Technology and Engineering, General Chemical Engineering, HYDROGELS, POLYMER, General Chemistry, F-19, Chemistry, ABA, BIODISTRIBUTION, Materials Chemistry, NANOPARTICLES, DRUG-DELIVERY, TEMPERATURE, BEHAVIOR, MRI
Abstract

Fluorine-19 magnetic resonance imaging (19F MRI) has emerged as a promising noninvasive diagnostic tool, broad-ening the diagnostic possibilities of commonly used proton MRI. Despite the potential of 19F MRI, an ideal tracer paving the way toward the entry of this method into common medical practice is yet to be developed. In this study, we report on a series of polymeric systems based on thermoresponsive poly[N-(2,2-difluoroethyl)acrylamide] (PDFEA), a polymer considered to be an ideal tracer for 19F MRI. The described systems are designed as BAB triblock copolymers, where B corresponds to thermores-ponsive PDFEA blocks and A is a hydrophilic poly(ethylene glycol) block. These BAB triblock copolymers are able to form nanoparticles in dilute aqueous solutions, which undergo a transition into physically cross-linked hydrogels upon increasing the polymer concentration. Since thermoresponsive particle-and hydrogel-based systems are applicable in a wide range of biomedical applications, we created a diagnostic system with potential therapeutic properties (theranostic) as a widely tunable platform through straightforward synthesis while serving a multitude of applications. We analyzed the effect of the BAB block ratio on the self-assembly, thermoresponsiveness, and mechanical properties of the studied hydrogels, together with their suitability for 19F MRI. Finally, their biocompatibility was assessed on a relevant cell line.

Published
2022

2. Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for

Author

Kristyna, Kolouchova, Ondrej, Groborz, Zulfiya, Cernochova, Aneta, Skarkova, Jan, Brabek, Daniel, Rosel, Pavel, Svec, Zenon, Starcuk, Miroslav, Slouf, and Martin, Hruby

Subjects
Drug Delivery Systems, Polymers, Nanoparticles, Pilot Projects, Precision Medicine, Reactive Oxygen Species, Ferric Compounds, Magnetic Resonance Imaging
Abstract

Fluorine-19 magnetic resonance imaging (

Published
2021

4. Thermoresponsive polymer system based on poly(N-vinylcaprolactam) intended for local radiotherapy applications

Author

Martin Hruby, Svetlana Lukáš Petrova, Peter Cernoch, Petr Stepanek, Jan Kučka, Zulfiya Cernochova, and Svetlana Petrova

Subjects
Materials science, Polymers, Brachytherapy, Biocompatible Materials, Iodine Radioisotopes, Neoplasms, Polymer chemistry, Materials Testing, Copolymer, Caprolactam, Humans, Thermoresponsive polymers in chromatography, Poly-N-vinylcaprolactam, Physiological saline, Solvent system, chemistry.chemical_classification, Radiation, Temperature, Water, Polymer, Biocompatible material, Chemical engineering, Local radiotherapy, chemistry, Solubility, Methacrylates, Tyrosine
Abstract

Brachytherapy represents effective local therapy of unresectable solid tumors with very few side effects. Radiolabeled thermoresponsive polymers offer almost noninvasive approach to brachytherapy applications. A radioiodinated, water-soluble, thermosensitive poly(N-vinylcaprolactam) (PVCL) polymer was prepared using two approaches. The direct copolymerization with N-methacryloyl- l -tyrosinamide, as well as end-capping of carboxy-terminated PVCL homopolymer with tyramine, were used. In both cases the product was successfully radiolabeled with 125I. The obtained polymers demonstrate cloud-point temperature (TC) values in the range of 33–35 °C in all the studied solvent systems (water, PBS (pH 7.4) and physiological saline solution). Above the cloud point temperature, the molecularly dissolved polymer is macroprecipitated from the solution. The TC values close to the human body temperature of this biocompatible poly(N-vinylcaprolactam) polymer makes it a promising material intended for local therapy of solid tumors.

Published
2014

5. Smart Poly(lactide)-b-poly(triethylene glycol methyl ether methacrylate) (PLA-b-PTEGMA) Block Copolymers: One-Pot Synthesis, Temperature Behavior, and Controlled Release of Paclitaxel

Author

Svetlana Lukáš Petrova, Martina Vragović, Ewa Pavlova, Zulfiya Černochová, Alessandro Jäger, Eliézer Jäger, and Rafał Konefał

Subjects
TEGMA, PLA, DLS, NMR, NOESY, LCST, Pharmacy and materia medica, RS1-441
Abstract

This paper introduces a new class of amphiphilic block copolymers created by combining two polymers: polylactic acid (PLA), a biocompatible and biodegradable hydrophobic polyester used for cargo encapsulation, and a hydrophilic polymer composed of oligo ethylene glycol chains (triethylene glycol methyl ether methacrylate, TEGMA), which provides stability and repellent properties with added thermo-responsiveness. The PLA-b-PTEGMA block copolymers were synthesized using ring-opening polymerization (ROP) and reversible addition–fragmentation chain transfer (RAFT) polymerization (ROP-RAFT), resulting in varying ratios between the hydrophobic and hydrophilic blocks. Standard techniques, such as size exclusion chromatography (SEC) and 1H NMR spectroscopy, were used to characterize the block copolymers, while 1H NMR spectroscopy, 2D nuclear Overhauser effect spectroscopy (NOESY), and dynamic light scattering (DLS) were used to analyze the effect of the hydrophobic PLA block on the LCST of the PTEGMA block in aqueous solutions. The results show that the LCST values for the block copolymers decreased with increasing PLA content in the copolymer. The selected block copolymer presented LCST transitions at physiologically relevant temperatures, making it suitable for manufacturing nanoparticles (NPs) and drug encapsulation-release of the chemotherapeutic paclitaxel (PTX) via temperature-triggered drug release mechanism. The drug release profile was found to be temperature-dependent, with PTX release being sustained at all tested conditions, but substantially accelerated at 37 and 40 °C compared to 25 °C. The NPs were stable under simulated physiological conditions. These findings demonstrate that the addition of hydrophobic monomers, such as PLA, can tune the LCST temperatures of thermo-responsive polymers, and that PLA-b-PTEGMA copolymers have great potential for use in drug and gene delivery systems via temperature-triggered drug release mechanisms in biomedicine applications.

Published
2023
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6. Potentiometric Performance of Ion-Selective Electrodes Based on Polyaniline and Chelating Agents: Detection of Fe2+ or Fe3+ Ions

Author

Rimeh Ismail, Ivana Šeděnková, Zulfiya Černochová, Iryna Romanenko, Ognen Pop-Georgievski, Martin Hrubý, and Elena Tomšík

Subjects
sensor of Fe2+ or Fe3+ ions, non-biofouling layer, poly(2-methyl-2-oxazoline)s, potentiometry, analysis, Biotechnology, TP248.13-248.65
Abstract

We constructed a sensor for the determination of Fe2+ and/or Fe3+ ions that consists of a polyaniline layer as an ion-to-electron transducer; on top of it, chelating molecules are deposited (which can selectively chelate specific ions) and protected with a non-biofouling poly(2-methyl-2-oxazoline)s layer. We have shown that our potentiometric sensing layers show a rapid response to the presence of Fe2+ or Fe3+ ions, do not experience interference with other ions (such as Cu2+), and work in a biological environment in the presence of bovine serum albumin (as a model serum protein). The sensing layers detect iron ions in the concentration range from 5 nM to 50 µM.

Published
2022
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