Search

Abstract Objective Kawasaki disease (KD) can lead to permanent damage to coronary structures, the pathogenesis of which remains unknown. This experiment was designed to investigate whether miR‐223‐3p secreted in the serum of KD patients affects the proliferation and apoptosis of HCAECs in KD by regulating FOXP3. Methods Blood samples were collected in acute febrile phase of KD, after IVIG treatment, and from healthy controls. Transfected into HCAECs cells by synthetic FOXP3 siRNA/NC. A co‐culture system was established between HCAECs cells transfected with FOXP3 siRNA/NC and THP1 cells added with three sera. Results Compared with the control group, the expressions of miR‐223‐3p, RORγt, and Th17 in serum of KD patients were significantly upregulated, and the expressions of TGF‐β1, FOXP3 and Treg were significantly downregulated. At the same time, the levels of IL‐6, IL‐17, and IL‐23 were significantly increased, and the levels of IL‐10 and FOXP3 were significantly decreased. After IVIG treatment, the patient's above results were reversed. The serum of KD patients increased the expression of miR‐223‐3p and inhibited the expression of FOXP3 in HCAECs cells. IVIG serum is the opposite. Overexpression of miR‐223‐3p also promoted the apoptosis of HCAECs. In addition, serum from KD patients promoted apoptosis, whereas serum after IVIG treatment inhibited apoptosis. KD patient serum downregulated the expression of FOXP3, Bcl2, TGF‐β1 and IL‐10 in cells, and upregulated the expression of caspase3, Bax, IL‐17, IL‐6, and IL‐23. The opposite results were obtained with IVIG‐treated sera. Conclusion miR‐223‐3p secreted in serum of KD patients can regulate the expression of FOXP3 and affect the proliferation, apoptosis, and inflammation of cells.

Dissolved organic matter extracted from compost tea, can be regarded as alternatives to inorganic fertilizers as well to be used as a washing agent for heavy metal polluted soil. However, the composition and quality of compost tea produced under different extraction time are still unknown. The objective of the current study was set to explore the influence of different extraction time (i.e., 6, 12, and 24 h) on the composition, quality, and copper binding capacity of compost tea originated from pig manure compost. The results indicated that the extraction time obviously influenced the phenolic, aromatic carboxylic, and polycyclic aromatic groups of compost tea. In addition, the compost tea undergo the shorter extraction time (i.e., 6 and 12 h) contained more protein and humic-like compositions. Among the all treatments tested herein, the compost tea produced from 12 h extraction time obviously exhibited higher aromaticity, molecular weight, and humification degree than other two treatments. The highest stability constant value (log KM) was observed for the shortest extraction time, i.e., 6.08. According to the quality measures and copper binding capability, shorter extraction times (i.e., 6 and 12 h) would be suggested for compost tea production from pig manure compost.

Copyright of Agricultural Science & Technology is the property of Hunan Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)