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10. Mechanistic insights into sulfur source-driven physiological responses and metabolic reorganization in the fuel-biodesulfurizing Rhodococcus qingshengii IGTS8

Author

Zumsteg, Julie, primary, Hirschler, Aurélie, additional, Carapito, Christine, additional, Maurer, Loïc, additional, Villette, Claire, additional, Heintz, Dimitri, additional, Dahl, Christiane, additional, El Nayal, Ashraf, additional, Sangal, Vartul, additional, Mahmoud, Huda, additional, Van Dorsselaer, Alain, additional, and Ismail, Wael, additional

Published
2023
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21. Biodesulfurization Induces Reprogramming of Sulfur Metabolism in Rhodococcus qingshengii IGTS8: Proteomics and Untargeted Metabolomics

Author

Hirschler, Aurélie, primary, Carapito, Christine, additional, Maurer, Loïc, additional, Zumsteg, Julie, additional, Villette, Claire, additional, Heintz, Dimitri, additional, Dahl, Christiane, additional, Al-Nayal, Ashraf, additional, Sangal, Vartul, additional, Mahmoud, Huda, additional, Van Dorsselaer, Alain, additional, and Ismail, Wael, additional

Published
2021
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22. Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Author

Rose, Jocelyn K.C., Kriegshauser, Lucie, Knosp, Samuel, Grienenberger, Etienne, Tatsumi, Kanade, Gütle, Desirée, Sørensen, Iben, Herrgott, Laurence, Zumsteg, Julie, Rose, Jocelyn, Reski, Ralf, Werck-Reichhart, Danièle, Renault, Hugues, Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), University of Freiburg [Freiburg], Cornell University, Cornell University [New York], and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
0106 biological sciences, Acylation, Physcomitrella, [SDV]Life Sciences [q-bio], Mutant, Shikimic Acid, Plant Science, Bryophyta, Saccharomyces cerevisiae, Genes, Plant, Models, Biological, 01 natural sciences, Gene Expression Regulation, Enzymologic, Conserved sequence, Evolution, Molecular, 03 medical and health sciences, Marchantia polymorpha, Phenols, Arabidopsis thaliana, Transferase, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Gene, Conserved Sequence, Phylogeny, Research Articles, 030304 developmental biology, 0303 health sciences, Phenylpropanoid, biology, food and beverages, Cell Biology, 15. Life on land, biology.organism_classification, Recombinant Proteins, Yeast, Kinetics, Metabolic pathway, Biochemistry, Biocatalysis, Embryophyta, Acyltransferases, 010606 plant biology & botany
Abstract

The plant phenylpropanoid pathway generates a major class of specialized metabolites and precursors of essential extracellular polymers that initially appeared upon plant terrestrialization. Despite its evolutionary significance, little is known about the complexity and function of this major metabolic pathway in extant bryophytes, which represent the non-vascular stage of embryophyte evolution. Here, we report that the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE (HCT) gene that plays a critical function in the phenylpropanoid pathway during seed plant development, is functionally conserved in Physcomitrium patens (Physcomitrella), in the moss lineage of bryophytes. Phylogenetic analysis indicates that bonafide HCT function emerged in the progenitor of embryophytes. In vitro enzyme assays, moss phenolic pathway reconstitution in yeast and in planta gene inactivation coupled to targeted metabolic profiling, collectively indicate that P. patens HCT (PpHCT), similar to tracheophyte HCT orthologs, uses shikimate as a native acyl acceptor to produce a p-coumaroyl-5-O-shikimate intermediate. Phenotypic and metabolic analyses of loss-of-function mutants show that PpHCT is necessary for the production of caffeate derivatives, including previously reported caffeoyl-threonate esters, and for the formation of an intact cuticle. Deep conservation of HCT function in embryophytes is further suggested by the ability of HCT genes from P. patens and the liverwort Marchantia polymorpha to complement an Arabidopsis thaliana CRISPR/Cas9 hct mutant, and by the presence of phenolic esters of shikimate in representative species of the three bryophyte lineages.

Published
2021

23. Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE 2 HYDROXYCINNAMOYL TRANSFERASE is evolutionarily 3 conserved in embryophytes

Author

Kriegshauser, Lucie, Knosp, Samuel, Grienenberger, Etienne, Gütle, Désirée, Sørensen, Iben, Herrgott, Laurence, Zumsteg, Julie, Rose, Jocely, Reski, Ralf, Werck Reichhart, Daniele, Renault, Hugues, Institut de biologie moléculaire des plantes (IBMP), and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021
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24. Nitrate-regulated growth processes involve activation of gibberellin pathway

Author

Camut, Lucie, primary, Gallova, Barbora, additional, Jilli, Lucas, additional, Sirlin-Josserand, Mathilde, additional, Carrera, Esther, additional, Sakvarelidze-Achard, Lali, additional, Zumsteg, Julie, additional, Heintz, Dimitri, additional, Ruffel, Sandrine, additional, Krouk, Gabriel, additional, Thomas, Stephen G., additional, Hedden, Peter, additional, Phillips, Andrew L., additional, Davière, Jean-Michel, additional, and Achard, Patrick, additional

Published
2021
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25. Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Author

Kriegshauser, Lucie, primary, Knosp, Samuel, additional, Grienenberger, Etienne, additional, Tatsumi, Kanade, additional, Gütle, Desirée D, additional, Sørensen, Iben, additional, Herrgott, Laurence, additional, Zumsteg, Julie, additional, Rose, Jocelyn K C, additional, Reski, Ralf, additional, Werck-Reichhart, Danièle, additional, and Renault, Hugues, additional

Published
2021
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27. Pioglitazone improves deficits ofFmr1-KO mouse model of Fragile X syndrome by interfering with excessive diacylglycerol signaling

Author

Geoffroy, Andréa, primary, Habbas, Karima, additional, Zambo, Boglarka, additional, Schramm, Laetitia, additional, Duchon, Arnaud, additional, Flatter, Eric, additional, Fouillen, Laetitia, additional, Zumsteg, Julie, additional, Heintz, Dimitri, additional, Mandel, Jean-Louis, additional, Hérault, Yann, additional, and Moine, Hervé, additional

Published
2020
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28. Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes

Author

Kriegshauser, Lucie, primary, Knosp, Samuel, additional, Grienenberger, Etienne, additional, Tatsumi, Kanade, additional, Gütle, Desirée D., additional, Sørensen, Iben, additional, Herrgott, Laurence, additional, Zumsteg, Julie, additional, Rose, Jocelyn K.C., additional, Reski, Ralf, additional, Werck-Reichhart, Danièle, additional, and Renault, Hugues, additional

Published
2020
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29. Environmental exposure to phthalates and dementia with Lewy bodies: contribution of metabolomics

Author

Agin, Arnaud, primary, Blanc, Frédéric, additional, Bousiges, Olivier, additional, Villette, Claire, additional, Philippi, Nathalie, additional, Demuynck, Catherine, additional, Martin-Hunyadi, Catherine, additional, Cretin, Benjamin, additional, Lang, Sabine, additional, Zumsteg, Julie, additional, Namer, Izzie Jacques, additional, and Heintz, Dimitri, additional

Published
2020
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31. Comparative patterns of modified nucleotides in individual tRNA species from a mesophilic and two thermophilic archaea

Author

Wolff, Philippe, Villette, Claire, Zumsteg, Julie, Heintz, Dimitri, Antoine, Laura, Chane-Woon-Ming, Béatrice, Droogmans, Louis, Grosjean, Henri, Westhof, Eric, Wolff, Philippe, Villette, Claire, Zumsteg, Julie, Heintz, Dimitri, Antoine, Laura, Chane-Woon-Ming, Béatrice, Droogmans, Louis, Grosjean, Henri, and Westhof, Eric

Abstract

To improve and complete our knowledge of archaeal tRNA modification patterns, we have identified and compared the modification pattern (type and location) in tRNAs of three very different archaeal species, Methanococcus maripaludis (a mesophilic methanogen), Pyrococcus furiosus (a hyperthermophile thermococcale), and Sulfolobus acidocaldarius (an acidophilic thermophilic sulfolobale). Most abundant isoacceptor tRNAs (79 in total) for each of the 20 amino acids were isolated by two-dimensional gel electrophoresis followed by in-gel RNase digestions. The resulting oligonucleotide fragments were separated by nanoLC and their nucleotide content analyzed by mass spectrometry (MS/MS). Analysis of total modified nucleosides obtained from complete digestion of bulk tRNAs was also performed. Distinct base- and/or ribose-methylations, cytidine acetylations, and thiolated pyrimidines were identified, some at new positions in tRNAs. Novel, some tentatively identified, modifications were also found. The least diversified modification landscape is observed in the mesophilic Methanococcus maripaludis and the most complex one in Sulfolobus acidocaldarius Notable observations are the frequent occurrence of ac4C nucleotides in thermophilic archaeal tRNAs, the presence of m7G at positions 1 and 10 in Pyrococcus furiosus tRNAs, and the use of wyosine derivatives at position 37 of tRNAs, especially those decoding U1- and C1-starting codons. These results complete those already obtained by others with sets of archaeal tRNAs from Methanocaldococcus jannaschii and Haloferax volcanii., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2020

32. Broad‐spectrum stress tolerance conferred by suppressing jasmonate signaling attenuation in Arabidopsis JASMONIC ACID OXIDASE mutants.

Author

Marquis, Valentin, Smirnova, Ekaterina, Graindorge, Stéfanie, Delcros, Pauline, Villette, Claire, Zumsteg, Julie, Heintz, Dimitri, and Heitz, Thierry

Subjects
JASMONIC acid, JASMONATE, SUPPRESSOR mutation, ABSCISIC acid, ARABIDOPSIS, DROUGHT tolerance
Abstract

SUMMARY: Jasmonate signaling for adaptative or developmental responses generally relies on an increased synthesis of the bioactive hormone jasmonoyl‐isoleucine (JA‐Ile), triggered by environmental or internal cues. JA‐Ile is embedded in a complex metabolic network whose upstream and downstream components strongly contribute to hormone homeostasis and activity. We previously showed that JAO2, an isoform of four Arabidopsis JASMONIC ACID OXIDASES, diverts the precursor jasmonic acid (JA) to its hydroxylated form HO‐JA to attenuate JA‐Ile formation and signaling. Consequently, JAO2‐deficient lines have elevated defenses and display improved tolerance to biotic stress. Here we further explored the organization and regulatory functions of the JAO pathway. Suppression of JAO2 enhances the basal expression of nearly 400 JA‐regulated genes in unstimulated leaves, many of which being related to biotic and abiotic stress responses. Consistently, non‐targeted metabolomic analysis revealed the constitutive accumulation of several classes of defensive compounds in jao2‐1 mutant, including indole glucosinolates and breakdown products. The most differential compounds were agmatine phenolamides, but their genetic suppression did not alleviate the strong resistance of jao2‐1 to Botrytis infection. Furthermore, jao2 alleles and a triple jao mutant exhibit elevated survival capacity upon severe drought stress. This latter phenotype occurs without recruiting stronger abscisic acid responses, but relies on enhanced JA‐Ile signaling directing a distinct survival pathway with MYB47 transcription factor as a candidate mediator. Our findings reveal the selected spectrum of JA responses controlled by the JAO2 regulatory node and highlight the potential of modulating basal JA turnover to pre‐activate mild transcriptional programs for multiple stress resilience. [ABSTRACT FROM AUTHOR]

Published
2022
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33. MOESM8 of Transcriptome analysis of Pueraria candollei var. mirifica for gene discovery in the biosyntheses of isoflavones and miroestrol

Author

Nithiwat Suntichaikamolkul, Kittitya Tantisuwanichkul, Pinidphon Prombutara, Khwanlada Kobtrakul, Zumsteg, Julie, Siriporn Wannachart, Schaller, Hubert, Yamazaki, Mami, Saito, Kazuki, Wanchai De-Eknamkul, Sornkanok Vimolmangkang, and Supaart Sirikantaramas

Subjects
Data_FILES
Abstract

Additional file 8: Table S2. Annotation statistics.

Published
2019
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34. Additional file 5: of Characterization of Jasmonoyl-Isoleucine (JA-Ile) Hormonal Catabolic Pathways in Rice upon Wounding and Salt Stress

Author

Hazman, Mohamed, Sühnel, Martin, Schäfer, Sandra, Zumsteg, Julie, Lesot, Agnès, Fréderic Beltran, Marquis, Valentin, Herrgott, Laurence, Miesch, Laurence, Riemann, Michael, and Heitz, Thierry

Abstract

Figure S5. SDS-PAGE analysis of recombinant AH8 and AH7 protein purification. AH8 and AH7 coding sequences were cloned in pHMGWA to be expressed as His-MBP-AH fusion proteins. a: Soluble AH8 bacterial lysate was submitted successively to amylose affinity (MBP-trap) followed by gel filtration chromatography. b: soluble AH7 bacterial lysate was submitted to immobilized metal-affinity chromatography (His-Trap). (DOCX 97 kb)

Published
2019
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35. Additional file 4: of Characterization of Jasmonoyl-Isoleucine (JA-Ile) Hormonal Catabolic Pathways in Rice upon Wounding and Salt Stress

Author

Hazman, Mohamed, Sühnel, Martin, Schäfer, Sandra, Zumsteg, Julie, Lesot, Agnès, Fréderic Beltran, Marquis, Valentin, Herrgott, Laurence, Miesch, Laurence, Riemann, Michael, and Heitz, Thierry

Subjects
food and beverages
Abstract

Figure S4. Expression profile of OsJAR2 upon salinity stress and leaf wounding in WT and jar1–1 seedlings. A: Ten-days old seedlings were submitted to leaf wounding and 2nd leaf was harvested at the indicated time points. Total RNA was submitted to RT-qPCR with JAR2-specific primers. B: Ten-days old seedlings were submitted to 100 mM NaCl stress and 2nd leaf was harvested at the indicated time points. Total RNA was submitted to RT-qPCR to reveal JAR2-specific expression. Values are means and SD from 3 independent biological replicates. (DOCX 48 kb)

Published
2019
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36. Additional file 3: of Characterization of Jasmonoyl-Isoleucine (JA-Ile) Hormonal Catabolic Pathways in Rice upon Wounding and Salt Stress

Author

Hazman, Mohamed, Sühnel, Martin, Schäfer, Sandra, Zumsteg, Julie, Lesot, Agnès, Fréderic Beltran, Marquis, Valentin, Herrgott, Laurence, Miesch, Laurence, Riemann, Michael, and Heitz, Thierry

Subjects
fungi, food and beverages
Abstract

Figure S3. OsAH gene expression upon leaf wounding. Ten-days old seedlings were submitted to leaf wounding and 2nd leaf was harvested at the indicated time points. Total RNA was submitted to RT-qPCR with AH-specific primers. Relative expression was determined using Actin as reference gene. Values are means and SD from 3 replicate determinations. (DOCX 57 kb)

Published
2019
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37. Additional file 1: of Characterization of Jasmonoyl-Isoleucine (JA-Ile) Hormonal Catabolic Pathways in Rice upon Wounding and Salt Stress

Author

Hazman, Mohamed, Sühnel, Martin, Schäfer, Sandra, Zumsteg, Julie, Lesot, Agnès, Fréderic Beltran, Marquis, Valentin, Herrgott, Laurence, Miesch, Laurence, Riemann, Michael, and Heitz, Thierry

Abstract

Figure S1. Arabidopsis simplified jasmonate metabolic pathway. JA-Ile oxidative and cleavage pathways are shown in yellow and blue backgrounds, respectively. (DOCX 48 kb)

Published
2019
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39. Additional file 2: of Characterization of Jasmonoyl-Isoleucine (JA-Ile) Hormonal Catabolic Pathways in Rice upon Wounding and Salt Stress

Author

Hazman, Mohamed, Sühnel, Martin, Schäfer, Sandra, Zumsteg, Julie, Lesot, Agnès, Fréderic Beltran, Marquis, Valentin, Herrgott, Laurence, Miesch, Laurence, Riemann, Michael, and Heitz, Thierry

Subjects
fungi, food and beverages
Abstract

Figure S2. OsCYP94 gene expression upon leaf wounding. Ten-days old seedlings were submitted to leaf wounding and 2nd leaf was harvested at the indicated time points. Total RNA was submitted to RT-qPCR with specific primers for selected OsCYP94 genes (as indicated in Fig. 1). Relative expression was determined using Actin as reference gene. Values are means and SD from 3 replicate determinations. (DOCX 72 kb)

Published
2019
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41. Transcriptome analysis of Pueraria candollei var. mirifica for gene discovery in the biosyntheses of isoflavones and miroestrol

Author

Suntichaikamolkul, Nithiwat, primary, Tantisuwanichkul, Kittiya, additional, Prombutara, Pinidphon, additional, Kobtrakul, Khwanlada, additional, Zumsteg, Julie, additional, Wannachart, Siriporn, additional, Schaller, Hubert, additional, Yamazaki, Mami, additional, Saito, Kazuki, additional, De-eknamkul, Wanchai, additional, Vimolmangkang, Sornkanok, additional, and Sirikantaramas, Supaart, additional

Published
2019
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42. De novo transcriptome assembly reveals putative biosynthetic genes involved in the biosyntheses of isoflavones and miroestrol in Pueraria candollei var. mirifica

Author

Suntichaikamolkul, Nithiwat, primary, Tantisuwanichkul, Kittiya, additional, Prombutara, Pinidphon, additional, Kobtrakul, Khwanlada, additional, Zumsteg, Julie, additional, Wannachart, Siriporn, additional, Schaller, Hubert, additional, Yamazaki, Mami, additional, Saito, Kazuki, additional, De-eknamkul, Wanchai, additional, Vimolmangkang, Sornkanok, additional, and Sirikantaramas, Supaart, additional

Published
2019
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44. RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis

Author

Lange, Heike, primary, Ndecky, Simon Y. A., additional, Gomez-Diaz, Carlos, additional, Pflieger, David, additional, Butel, Nicolas, additional, Zumsteg, Julie, additional, Kuhn, Lauriane, additional, Piermaria, Christina, additional, Chicher, Johana, additional, Christie, Michael, additional, Karaaslan, Ezgi S., additional, Lang, Patricia L. M., additional, Weigel, Detlef, additional, Vaucheret, Hervé, additional, Hammann, Philippe, additional, and Gagliardi, Dominique, additional

Published
2019
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45. Jasmonic Acid Oxidase 2 (JAO2) hydroxylates jasmonic acid and represses basal defense and resistance responses against Botrytis cinerea infection

Author

Smirnova, Ekaterina, Marquis, Valentin, Poirier, L, Aubert, Yann, Zumsteg, Julie, Menard, Rozenn, Miesch, Laurence, Heitz, T, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut de Chimie de Strasbourg, Centre National de la Recherche Scientifique (CNRS)-Université Louis Pasteur - Strasbourg I-Institut de Chimie du CNRS (INC), Laboratoire de Chimie Quantique, Centre National de la Recherche Scientifique (CNRS), and Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

46. Metabolomics - an overview. From basic principles to potential biomarkers (part 1)

Author

Agin, A, Heintz, Dimitri, Ruhland, E, Chao de La Barca, J M, Zumsteg, Julie, Mola, V, Gauchez, a S, Namer, Izzie-Jacques, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), and Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

48. Root-derived GA12contributes to temperature-induced shoot growth in Arabidopsis

Author

Camut, Lucie, Regnault, Thomas, Sirlin-Josserand, Mathilde, Sakvarelidze-Achard, Lali, Carrera, Esther, Zumsteg, Julie, Heintz, Dimitri, Leonhardt, Nathalie, Lange, Maria João Pimenta, Lange, Theo, Davière, Jean-Michel, and Achard, Patrick

Abstract

Plants are able to sense a rise in temperature of several degrees, and appropriately adapt their metabolic and growth processes. To this end, plants produce various signalling molecules that act throughout the plant body. Here, we report that root-derived GA12, a precursor of the bioactive gibberellins, mediates thermo-responsive shoot growth in Arabidopsis. Our data suggest that root-to-shoot translocation of GA12enables a flexible growth response to ambient temperature changes.

Published
2019
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50. The BRAHMA-associated SWI/SNF chromatin remodeling complex controls Arabidopsis seed quality and physiology.

Author

Wrona M, Zinsmeister J, Krzyszton M, Villette C, Zumsteg J, Mercier P, Neveu M, Sacharowski SP, Archacki R, Collet B, Buitink J, Schaller H, Swiezewski S, and Yatusevich R

Subjects
Plant Dormancy genetics, Chromosomal Proteins, Non-Histone metabolism, Chromosomal Proteins, Non-Histone genetics, Mutation genetics, Promoter Regions, Genetic genetics, Phenotype, Adenosine Triphosphatases, Arabidopsis genetics, Arabidopsis physiology, Arabidopsis metabolism, Seeds genetics, Seeds growth & development, Seeds metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Chromatin Assembly and Disassembly genetics, Gene Expression Regulation, Plant, Transcription Factors metabolism, Transcription Factors genetics
Abstract

The SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin remodeling complex is involved in various aspects of plant development and stress responses. Here, we investigated the role of BRM (BRAHMA), a core catalytic subunit of the SWI/SNF complex, in Arabidopsis thaliana seed biology. brm-3 seeds exhibited enlarged size, reduced yield, increased longevity, and enhanced secondary dormancy, but did not show changes in primary dormancy or salt tolerance. Some of these phenotypes depended on the expression of DOG1, a key regulator of seed dormancy, as they were restored in the brm-3 dog1-4 double mutant. Transcriptomic and metabolomic analyses revealed that BRM and DOG1 synergistically modulate the expression of numerous genes. Some of the changes observed in the brm-3 mutant, including increased glutathione levels, depended on a functional DOG1. We demonstrated that the BRM-containing chromatin remodeling complex directly controls secondary dormancy through DOG1 by binding and remodeling its 3' region, where the promoter of the long noncoding RNA asDOG1 is located. Our results suggest that BRM and DOG1 cooperate to control seed physiological properties and that BRM regulates DOG1 expression through asDOG1. This study reveals chromatin remodeling at the DOG1 locus as a molecular mechanism controlling the interplay between seed viability and dormancy., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)

Published
2024
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