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This study investigates acoustic radiation forces on spherical objects generated by Bessel beams in a viscous fluid. Radiation forces on elastic spheres and shells of different materials are examined using viscid expression with the thermoviscous correction included, and the results are then compared with numerical simulations based on the finite element method. The Stokes drag force for zero-order Bessel waves was theoretically derived, and in turn, a practical example of negative radiation forces is proposed and investigated together with the gravity, the buoyancy, and the drag force from acoustic streaming. It is found that the negative pulling force exists even including the positive forces from the other sources; however, the parameter regions for pulling forces are reduced especially for small objects. This work helps the further study of particle manipulations by acoustic Bessel beams in viscous fluids and also guides the experimental realization of acoustic tractor beams. [ABSTRACT FROM AUTHOR]

A deep neural network (DNN) was trained to generate a multiparametric ultrasound (mpUS) volume from four input ultrasound-based modalities (acoustic radiation force impulse [ARFI] imaging, shear wave elasticity imaging [SWEI], quantitative ultrasound-midband fit [QUS-MF], and B-mode) for the detection of prostate cancer. A DNN was trained using co-registered ARFI, SWEI, MF, and B-mode data obtained in men with biopsy-confirmed prostate cancer prior to radical prostatectomy (15 subjects, comprising 980,620 voxels). Data were obtained using a commercial scanner that was modified to allow user control of the acoustic beam sequences and provide access to the raw image data. For each subject, the index lesion and a non-cancerous region were manually segmented using visual confirmation based on whole-mount histopathology data. In a prostate phantom, the DNN increased lesion contrast-to-noise ratio (CNR) compared to a previous approach that used a linear support vector machine (SVM). In the in vivo test datasets (n = 15), the DNN-based mpUS volumes clearly portrayed histopathology-confirmed prostate cancer and significantly improved CNR compared to the linear SVM (2.79 ± 0.88 vs. 1.98 ± 0.73, paired-sample t -test p < 0.001). In a sub-analysis in which the input modalities to the DNN were selectively omitted, the CNR decreased with fewer inputs; both stiffness- and echogenicity-based modalities were important contributors to the multiparametric model. The findings from this study indicate that a DNN can be optimized to generate mpUS prostate volumes with high CNR from ARFI, SWEI, MF, and B-mode and that this approach outperforms a linear SVM approach. [ABSTRACT FROM AUTHOR]

A formal analytical solution of the acoustic radiation force on an elliptical cylinder arbitrarily positioned in a cylindrical quasi-Gaussian beam is developed. The scattering coefficients for the elliptical cylinder are obtained through forcing the acoustic field to meet the boundary condition at the surface of the cylinder, which depends on whether the elliptical cylinder is rigid or fluid. A numerical integration is performed for each partial-wave during the computations. Numerical simulations are provided with particular emphases on the amount of the shift from the center of the cylinder, the tilt incident angle, the aspect ratio of the cylinder and the beam waist of the incident beam. The radiation force on a fluid elliptical cylinder shows a more oscillatory behavior than a rigid one. The attractive transverse radiation force exists under certain conditions, proving the feasibility of designing lateral acoustic tweezers. A wider incident beam induces a stronger axial radiation force due to expansion of the cross section. The results are helpful for predicting the dynamic behaviors of cylindrical particles and guiding particle manipulation in the field of focused cylindrical beams. [ABSTRACT FROM AUTHOR]

Contactless microscale tweezers are highly effective tools for manipulating, patterning, and assembling bioparticles. However, current tweezers are limited in their ability to comprehensively manipulate bioparticles, providing only partial control over the six fundamental motions (three translational and three rotational motions). This study presents a joint subarray acoustic tweezers platform that leverages acoustic radiation force and viscous torque to control the six fundamental motions of single bioparticles. This breakthrough is significant as our manipulation mechanism allows for controlling the three translational and three rotational motions of single cells, as well as enabling complex manipulation that combines controlled translational and rotational motions. Moreover, our tweezers can gradually increase the load on an acoustically trapped cell to achieve controllable cell deformation critical for characterizing cell mechanical properties. Furthermore, our platform allows for three-dimensional (3D) imaging of bioparticles without using complex confocal microscopy by rotating bioparticles with acoustic tweezers and taking images of each orientation using a standard microscope. With these capabilities, we anticipate the JSAT platform to play a pivotal role in various applications, including 3D imaging, tissue engineering, disease diagnostics, and drug testing. The joint subarray acoustic tweezer platform offers 3D translation and rotation of bioparticles, enabling complex manipulation and cell deformation, with applications in tissue engineering, disease diagnostics, and drug testing. [ABSTRACT FROM AUTHOR]

Microparticle manipulation is a critical concern across various fields including microfabrication, flexible electronics and tissue engineering. Acoustic-activated sharp structures have been designed as simple and flexible tools to manipulate microparticles with their good compatibility, fast response, and broad tunability. However, there still lacks rational acoustic-structure design for effective energy concentration at the acoustic-activated sharp structures for microparticle manipulation. Here, we present the acoustic black hole (ABH) effect as enhancement for the acoustic micro-manipulator. It provides great reliability, simplicity and ease of use, supporting custom design of high-throughput patterning modes. Moreover, compared to commonly used configurations, such as cylindrical or conical microneedles, those microneedles with ABH profile exhibit superior acoustic energy focusing at the tip and induce stronger acoustofluidic effects. The average acoustic flow velocity induced by the ABH microneedle is 154 times greater than that of the conical one and 45 times greater than that of the cylindrical microneedle. Besides, the average acoustic radiation force (ARF) produced by the ABH microneedle against acrylic microparticles is about 319 times greater than that of the cylindrical one and 16 times greater than that of the conical one. These results indicate that ABH design significantly enhances microparticle manipulation. We demonstrate this concept with ABH effect enhanced microparticle manipulation and study the parameters influencing its performance including operating frequency, operating voltage and particle diameter. Furthermore, considering the flexibility of this system, we employ it for various patterning and high-throughput microparticle manipulation. This work paves the way for controllable microparticle manipulation, holding great potential for applications in microfabrication and biomedicine. [ABSTRACT FROM AUTHOR]

In recent years, there has been significant development in microfluidic devices for cell separation and sorting using acoustic methods in biomedical applications. The acoustic interparticle force (AIF) or the secondary acoustic radiation force arises from particle interactions with the scattered field of other particles, influencing particle motion at close ranges and facilitating optimal trapping and separation. This study analyzes a two-particle system consisting of a fixed particle and a white blood cell (WBC) within a standing acoustic field and creeping flow using fluid-structure interaction (FSI). To reduce computational costs by decoupling the acoustics and FSI, the acoustic pressure equation was solved on the frequency domain to calculate the total acoustic radiation force in each time step. Model accuracy was assessed by evaluating interparticle (AIF) and primary acoustic radiation force (ARF) on a polystyrene particle and comparing simulation results to analytical and experimental data. Results demonstrate the precise primary ARF computation, with discrepancies in AIF attributed to viscous losses near the particle surface. Moreover, the higher density of the fixed particle compared to WBCs induces significant acoustic interparticle attraction at close distances. Consequently, cell entrapment occurs through strong attraction and collision with fixed aluminum and silicon particles in creeping flow in all three Reynolds numbers 1.4 × 10−3, 2.1 × 10−3, and 3 × 10−3. Increasing Reynolds numbers augment the likelihood of cell separation from the fixed particle. These findings contribute to optimizing cell isolation and entrapment strategies. [ABSTRACT FROM AUTHOR]

Introduction: Numerous studies have shown that natural mechanical waves have the potential to assess the elastic properties of the myocardium. When the Aortic and Mitral valves close, a shear wave is produced, which provides insights into tissue stiffness. In addition, the Atrial Kick (AK) generates a wave similar to Pulse Waves (PWs) in arteries, providing another way to assess tissue stiffness. However, tissue anisotropy can also impact PW propagation, which is currently underexplored. This study aims to address this gap by investigating the impact of anisotropy on PW propagation in a phantom. Methods: Tube phantoms were created using Polyvinyl Alcohol (PVA). Anisotropy was induced between two sets of two freeze-thaw cycles by stretching and twisting the material. The study first tests and validates the procedure of making helical anisotropic vessel phantoms using the shear wave imaging technique (by estimating the shear wave speed at different probe angles). Using plane wave ultrasound tomography synchronized with a peristaltic pump, 3D high frame rate imaging is performed and used to detect the 3D propagation pattern of PW for each manufactured vessel phantom. Finally, the study attempts to extract the anisotropic coefficient of the vessel using pulse wave propagation angle. Results: The Shear wave imaging results obtained for the isotropic vessel show very similar values for each probe angle. On the contrary, the results obtained for the axial anisotropy vessel show a region with a higher shear wave speed at about 0°, corresponding to the long axis of the vessel. Finally, the results obtained for the helical anisotropy depicted increasing shear wave velocity value from -20° to 20°. For the axial phantom, the wavefront of the pulse wave is perpendicular to the long axis of the vessel, while oriented for the helical anisotropic vessels phantom. The pulse wave propagation angle increased with the number of twists made during the vessel manufacturing. Discussion: The results show that anisotropy can be induced in PVA vessel phantoms by stretching and twisting the material in freeze-thaw cycles. The findings also suggest that vessel anisotropy affects pulse wave propagation angles. Estimating the pulse wave propagation angle may be interesting in characterizing tissue anisotropy in organs where such waves are naturally present. [ABSTRACT FROM AUTHOR]

Anisotropic elasticity measurements of the retina are essential for retinal disease diagnosis and function assessment. Optical coherence elastography (OCE) is a high-resolution imaging technique for mapping the elasticity distribution of tissues. However, previous OCE measurements quantified the tissue elasticity in a single direction, resulting in a biased estimation of the elastic properties. In this study, we propose an OCE method with acoustic radiation force (ARF) excitation to map the retinal anisotropic elasticity in the depth and lateral directions. The axial elasticity was analyzed using the natural frequency of free vibration, and the lateral elasticity was quantified using the elastic wave velocity. After evaluating the feasibility of the OCE method on the phantoms, the anisotropic elasticity of ex vivo porcine retinas was mapped. The results show that the OCE method with ARF excitation can assess the elasticity in orthogonal directions and provide a comprehensive understanding of the elasticity of the anisotropic tissues. [ABSTRACT FROM AUTHOR]

Superhydrophobic surfaces (SHS) exhibit a pronounced ability to resist wetting. When immersed in water, water does not penetrate between the microstructures of the SHS. Instead, a thin layer of trapped gas remains, i.e., plastron. This fractional wetting is also known as the Cassie–Baxter state (CB). Impairment of superhydrophobicity occurs when water penetrates the plastron and, when complete wetting is achieved, a Wenzel state (W) results. Subsequent recovery back to CB state is one of the main challenges in the field of SHS wetting. Current methods for plastron recovery require complex mechanical or chemical integration, are time‐consuming or lack spatial control. Here an on‐demand, contact‐less approach for performing facile transitions between these wetting states at micrometer length scales is proposed. This is achieved by the use of acoustic radiation force (ARF) produced by high‐intensity focused ultrasound (HIFU). Switching from CB to W state takes <100 µs, while the local recovery back to CB state takes <45 s. To the best of authors knowledge, this is the first demonstration of ARF‐induced manipulation of the plastron enabling facile two‐way controlled switching of wetting states. [ABSTRACT FROM AUTHOR]

Acoustic levitation is frequently used for non-contact manipulation of objects and to study the impact of microgravity on physical and biological processes. While the force field produced by sound pressure lifts particles against gravity (primary acoustic force), multiple levitating objects in the same acoustic cavity interact via forces that arise from scattered sound (secondary acoustic forces). Current experimental techniques for obtaining these force fields are not well-suited for mapping the primary force field at high spatial resolution and cannot directly measure the secondary scattering force. Here, we introduce a method that can measure both acoustic forces in situ, including secondary forces in the near-field limit between arbitrarily shaped, closely spaced objects. Operating similarly to an atomic force microscope, the method inserts into the acoustic cavity a suitably shaped probe tip at the end of a long, flexible cantilever and optically detects its deflection. This makes it possible to measure forces with a resolution better than 50 nN and also to apply stress or strain in a controlled manner to manipulate levitated objects. We demonstrate this by extracting the acoustic potential present in a levitation cavity, directly measuring the acoustic scattering force between two objects, and applying tension to a levitated granular raft of acoustically bound particles in order to obtain the force–displacement curve for its deformation. [ABSTRACT FROM AUTHOR]

This report described the use of acoustic radiation force impulse (ARFI) elastography in the diagnosis of two cases of canine malignant peripheral nerve sheath tumors. Both patients presented with lameness in the thoracic limb and no response to previous clinical treatment or trauma history. Physical examination revealed muscular atrophy in one of the thoracic limbs, in both dogs. B-mode axillary ultrasound showed the presence of a predominantly hypoechogenic, heterogeneous, irregular, nonencapsulated nodule with adjacent reactivity, measuring approximately 20.1 mm x 15.4 mm in the first case and 18.3 mm x 10.7 mm in the second. ARFI elastography was performed, showing areas of significant stiffness, with an average shear wave velocity of 4.12 m/s (case 1) and 4.35 m/s (case 2), suggesting malignancy. In both cases, the affected limb was amputated, and the tumors sent for histopathological analysis, which were; subsequently, diagnosed as malignant tumors of the peripheral nerve sheath. The ultrasonographic methods used were essential in the diagnosis and therapeutic conduct. [ABSTRACT FROM AUTHOR]

Ultrasound‐directed self‐assembly (DSA) utilizes the acoustic radiation force associated with a standing ultrasound wave field to organize particles dispersed in a fluid medium into specific patterns. State‐of‐the‐art ultrasound DSA methods use single‐frequency ultrasound wave fields, which only allow organizing particles into simple, periodic patterns, or require a large number of ultrasound transducers to assemble complex patterns. In contrast, this work introduces multi‐frequency ultrasound wave fields to organize particles into complex patterns. A method is theoretically derived to determine the operating parameters (frequency, amplitude, phase) of any arrangement of ultrasound transducers, required to assemble spherical particles dispersed in a fluid medium into specific patterns, and experimentally validated for a system with two frequencies. The results show that multi‐frequency compared to single‐frequency ultrasound DSA enables the assembly of complex patterns of particles with substantially fewer ultrasound transducers. Additionally, the method does not incur a penalty in terms of accuracy, and it does not require custom hardware for each different pattern, thus offering reconfigurability, which contrasts, e.g., acoustic holography. Multi‐frequency ultrasound DSA can spur progress in a myriad of engineering applications, including the manufacturing of multi‐functional polymer matrix composite materials that derive their structural, electric, acoustic, or thermal properties from the spatial organization of particles in the matrix. [ABSTRACT FROM AUTHOR]

Acoustic tweezers have gained substantial interest in biology, engineering, and materials science for their label-free, precise, contactless, and programmable manipulation of small objects. However, acoustic tweezers cannot independently manipulate multiple microparticles simultaneously. This study introduces acousto-dielectric tweezers capable of independently manipulating multiple microparticles and precise control over intercellular distances and cyclical cell pairing and separation for detailed cell-cell interaction analysis. Our acousto-dielectric tweezers leverage the competition between acoustic radiation forces, generated by standing surface acoustic waves (SAWs), and dielectrophoretic (DEP) forces, induced by gradient electric fields. Modulating these fields allows for the precise positioning of individual microparticles at points where acoustic radiation and DEP forces are in equilibrium. This mechanism enables the simultaneous movement of multiple microparticles along specified paths as well as cyclical cell pairing and separation. We anticipate our acousto-dielectric tweezers to have enormous potential in colloidal assembly, cell-cell interaction studies, disease diagnostics, and tissue engineering. [ABSTRACT FROM AUTHOR]

Introduction: Chronic kidney disease is a progressive condition that affects >10% of the general population worldwide and has emerged as one of the leading causes of mortality worldwide. The diagnosis and staging are based on the clinical and biochemical assays. Shear wave elastography is an emerging ultrasound technique that has a potential role in assessing the severity of CKD. However, it is still in the preliminary phase and has yet to be incorporated into the guidelines. Hence, the main purpose of our study is to evaluate the role of shear wave elastography utilizing acoustic radiation force impulse technique in assessing the severity of CKD and to evaluate whether there is any correlation between renal SWV values with serum creatinine, estimated glomerular filtration rate, and renal resistive index. Method: A prospective observational study of comparative cross-sectional design with analytical component comparing two groups, one group being clinical and biochemically proven case of CKD and the other group comprising non-CKD patients. There were 106 patients, with 52 being known cases of CKD and the other 54 being non-CKD patients. Shear wave velocity and renal resistive index were measured in these two groups, and a correlation between SWV, RRI, serum creatinine, and eGFR was made. Results: A cutoff kidney shear wave speed value of 2.49 m/s could be used to discriminate the diseased group from the diseased group with a sensitivity, specificity, PPV, NPV, and accuracy of 84.62%, 87.04%, 86.27%, 85.45% and 85.85% respectively and the mean shear wave velocity decreased with the decrease in eGFR and the mean SWV among the CKD groups were 2.51 ±0.26 m/s for stage 2, 2.34 ±0.25 m/s for stage 3A and 2.24±0.28 m/s for stage 3B and 1.98±0.21 m/s for stage 4 of CKD. There is also a linear positive correlation between SWV and renal resistive index. Renal shear wave velocity values decreased with the increase in serum creatinine and a decrease in the estimated GFR. There was a statistically significant difference between renal SWV values in patients with an estimated GFR greater than 90 mL/min/1.73m2 compared to patients with stage 4 or 5 CKD (estimated GFR, <30 mL/min/1.73m2). Conclusion: Our study showed that renal shear wave velocity (SWV) values estimated by ARFI elastography showed a statistically significant difference between patients with CKD and those with normal renal functions. In patients with CKD, the SWV values showed a progressive decline with deterioration of eGFR vis-a-vis an increase in the severity of CKD. A cut-off SWV value of 2.49 m/s or less could differentiate the CKD group from the non-CKD group while having good sensitivity and specificity. Our study also showed that along with RI, SWV values were also found to be helpful in predicting the progression of CKD. Thus, ARFI elastography along with renal Doppler (in addition to eGFR ) evaluation is a potential adjunct imaging method for assessing patients with CKD, especially in the case of diabetic nephropathy where conventional US findings can be equivocal. [ABSTRACT FROM AUTHOR]

An analytical solution is developed for the acoustic radiation force and torque caused by an arbitrary sound field that is incident on a compressible spheroid of any size near a planar boundary that is either rigid or pressure release. The analysis is an extension of a recent solution for a compressible sphere near a planar boundary [Simon and Hamilton, J. Acoust. Soc. Am. 153, 627–642 (2023)]. Approximations that account for a boundary formed by a two-fluid interface may be incorporated as in the previous analysis for a sphere. The present solution is based on expansions of the total acoustic pressure field in spheroidal wave functions and the use of addition theorems. Verification of the solution is accomplished by comparison with a finite element model. Examples are presented for incident fields that are either plane or spherical waves. Effects resulting from the presence of the boundary are studied by comparing the full theory with a simplified model in which multiple scattering is neglected. Numerical implementation of the proposed solution is also discussed. [ABSTRACT FROM AUTHOR]

We present a numerical study to investigate the efficacy of impedance boundary conditions in capturing the interfacial dynamics of a particle subjected to an acoustic field and study the concomitant time-averaged acoustic streaming and radiation force fields. While impedance boundary conditions have been utilized to represent fluid–solid interface in acoustofluidics, such models assume the solid material to be locally reactive to the acoustic waves. However, there is a limited understanding of when this assumption holds true, raising concerns about the suitability of impedance boundary conditions. Here, we systematically investigate the applicability of impedance boundary conditions by comparing the predictions of an impedance boundary approach against a fully coupled fluid–solid model. We contrast the oscillation profiles of the fluid–solid interface predicted by the two models. We consider different scatterer materials to identify the extent to which the differences in interfacial dynamics impact the time-averaged fields and highlight the divergence within the predictions of the two models. Our findings indicate that, although impedance boundary conditions can yield qualitatively similar results to the full model in certain situations, the predictions from the two models generally differ both qualitatively and quantitatively. These results underscore the importance of exercising caution when applying these boundary conditions to model general acoustofluidic systems. [ABSTRACT FROM AUTHOR]

Acoustofluidics has shown great potential in enabling on‐chip technologies for driving liquid flows and manipulating particles and cells for engineering, chemical, and biomedical applications. To introduce on‐demand liquid sample processing and micro/nano‐object manipulation functions to wearable and embeddable electronics, wireless acoustofluidic chips are highly desired. This paper presents wireless acoustofluidic chips to generate acoustic waves carrying sufficient energy and achieve key acoustofluidic functions, including arranging particles and cells, generating fluid streaming, and enriching in‐droplet particles. To enable these functions, the wireless acoustofluidic chips leverage mechanisms, including inductive coupling‐based wireless power transfer (WPT), frequency multiplexing‐based control of multiple acoustic waves, and the resultant acoustic radiation and drag forces. For validation, the wirelessly generated acoustic waves are measured using laser vibrometry when different materials (e.g., bone, tissue, and hand) are inserted between the WPT transmitter and receiver. Moreover, the wireless acoustofluidic chips successfully arrange nanoparticles into different patterns, align cells into parallel pearl chains, generate streaming, and enrich in‐droplet microparticles. This research is anticipated to facilitate the development of embeddable wireless on‐chip flow generators, wearable sensors with liquid sample processing functions, and implantable devices with flow generation and acoustic stimulation abilities for engineering, veterinary, and biomedical applications. [ABSTRACT FROM AUTHOR]

Summary: Background: Obesity impacts the diagnostic accuracy of shear wave elastography (SWE). A deep abdominal ultrasound transducer (DAX) capable of point (pSWE) and two‐dimensional (2D)‐SWE has recently been introduced to address this issue. Methods: We performed a prospective study in a cohort of mostly patients with obesity undergoing liver biopsy with a high prevalence of metabolic dysfunction‐associate steatotic liver disease (MASLD). Liver stiffness measurement (LSM) was measured using vibration‐controlled transient elastography (VCTE), as well as pSWE and 2D SWE on the standard (5C1) and the DAX transducers. Results: We included 129 patients with paired LSM and liver biopsy: median age 44.0 years, 82 (63.6%) women, median BMI: 43.2 kg/m2. Histologic fibrosis stages: F0: N = 55 (42.6%), F1: N = 14 (10.9%), F2: N = 50 (38.8%), F3: N = 2 (1.6%), F4: N = 8 (6.2%). VCTE‐LSM failed (N = 13) or were unreliable (IQR/median ≤30% in ≥7.1 kPa, N = 14) in 20.9% of patients. The Pearson correlation of reliable VCTE‐LSM with both pSWE and 2D SWE was strong (all >0.78). The diagnostic accuracy for all LSM techniques was poor for significant fibrosis (≥F2, AUC: 0.54–0.63); however, it was good to excellent for advanced fibrosis (≥F3, AUC: 0.87–0.99) and cirrhosis (F4, AUC: 0.86–1.00). In intention‐to‐diagnose analysis, pSWE on DAX was significantly superior to VCTE‐LSM. Conclusions: pSWE‐ and 2D‐SWE enable the non‐invasive identification of advanced fibrosis and cirrhosis in patients with obese MASLD. The use of the DAX transducer for acoustic radiation force imaging (ARFI)‐LSM avoids technical failures in an obese population and subsequently offers advantages over VCTE‐LSM for the evaluation of fibrosis in an obese MASLD population at risk for fibrosis. [ABSTRACT FROM AUTHOR]

Liquid surfaces can be depressed by applying acoustic radiation force. The balance between the acoustic radiation force, surface tension force, and buoyant force sustains the stable dimple depression. Beyond a certain threshold, higher acoustic radiation force leads to instability and bubble formation. The bubble size is determined by the acoustic radiation force and the liquid surface tension. Effective management of bubble generation can be achieved by controlling acoustic radiation waves. A novel method for creating depression on liquid surfaces and generating bubbles is described, which requires neither gas supply nor direct contact with equipment. [ABSTRACT FROM AUTHOR]

Recently researchers often normalize the radiation force on spheres in standing waves in inviscid fluids using an acoustic contrast factor (typically denoted by Φ) that is independent of kR where k is the wave number and R is the sphere radius. An alternative normalization uses a function Ys that depends on kR. Here, standard results for Φ are extended as a power series in kR using prior Ys results. Also, new terms are found for fluid spheres and applied to the kR dependence of Φ for strongly responsive and weakly responsive examples. Partial-wave phase shifts are used in the derivation. [ABSTRACT FROM AUTHOR]

Manipulating objects with acoustics has been developed for hundreds of years since Chladni patterns in gaseous environments were exhibited. In recent decades, acoustic manipulation in microfluidics, known as acoustofluidics, has rapidly thrived and many sophisticated technologies were born. However, the basic background motion of particles under acoustic excitation is usually neglected and the classical Chladni patterns haven't been reproduced in an aqueous environment. In this study, we investigated the basic mechanism and the motion of suspended particles and sinking particles in a plain microchamber under low-frequency excitation (3-5 kHz). The mechanisms were clearly distinguished by comparing the differences among colored fluids, suspended particles, and sinking particles. The suspended particles rotated around the antinode with a speed up to 55.1 µm s-1 at 100 Vpp by the acoustic streaming and they approached each other by the secondary acoustic radiation force. The sinking particles concentrated at the node with a speed up to 22.3 µm s-1 at 100 Vpp by bouncing on the vibrating surface and the primary acoustic radiation force. We have reproduced the classical standard/inverse Chladni patterns in an aqueous environment for the first time, and they were leveraged to separate SiO2 particles with different sizes. The big particles with an average diameter of 9.68 µm were concentrated at the node while the small particles with an average diameter of 2.72 µm were collected at the antinode within 2 min. These results not only provide insightful perspectives of basic mechanisms, but also open up new possibilities for advanced acoustic tweezers. [ABSTRACT FROM AUTHOR]

Desmosomes are relatives of ancient cadherin-based junctions, which emerged late in evolution to ensure the structural integrity of vertebrate tissues by coupling the intermediate filament cytoskeleton to cell-cell junctions. Their ability to dynamically counter the contractile forces generated by actin-associated adherens junctions is particularly important in tissues under high mechanical stress, such as the skin and heart. Much more than the simple cellular 'spot welds' depicted in textbooks, desmosomes are in fact dynamic structures that can sense and respond to changes in their mechanical environment and external stressors like ultraviolet light and pathogens. These environmental signals are transmitted intracellularly via desmosome-dependent mechanochemical pathways that drive the physiological processes of morphogenesis and differentiation. This Cell Science at a Glance article and the accompanying poster review desmosome structure and assembly, highlight recent insights into how desmosomes integrate chemical and mechanical signaling in the epidermis, and discuss desmosomes as targets in human disease. [ABSTRACT FROM AUTHOR]

Objective: To investigate and quantify age-related changes in lower limb muscle stiffness in typically developing children and adolescents using acoustic radiation force impulse shear wave elastography. Materials and methods: Shear wave velocities of bilateral rectus femoris, tibialis anterior, and medial gastrocnemius muscles at rest were obtained in typically developing children and adolescents aged 3 to 18 years. The participants were classified into three age groups: Group 1 (children), 3 to 7 years old; Group 2, 8 to 12 (pre-adolescent); and Group 3 (adolescent), 13 to 18. The shear wave velocities of muscle were compared across the three age groups, as well as compared between right- and left-side limbs. The correlation between shear wave velocities and body weight or body mass index was assessed. Results: Of the 47 participants, 21 were in Group 1, 17 in Group 2, and 9 in Group 3. There were no significant differences among the three age groups' shear wave velocities of bilateral lower limb muscles, and no significant differences between right and left sides. There was no correlation between muscle stiffness and body weight or body mass index. Conclusion: The present pilot study applied acoustic radiation force impulse shear wave elastography to quantify lower limb muscle stiffness in typically developing children and adolescents aged 3 to 18 years, suggesting no marked change in muscle stiffness occurs as they develop. [ABSTRACT FROM AUTHOR]

Background: Non-alcoholic fatty liver disease (NAFLD) encompasses a heterogeneous spectrum ranging from simple steatosis to fibrosis and cirrhosis. Fibrosis, associated with long-term overall mortality and liver-related events, requires evaluation. Traditionally, liver biopsy has been the gold standard for diagnosing fibrosis. However, its invasive nature, potential complications, and sampling variability limit widespread use. Consequently, various non-invasive tests have been developed as alternatives for diagnosing fibrosis in NAFLD patients. Aim: This study aimed to compare the accuracy of non-invasive tests (NITs) and evaluate the diagnostic accuracy of acoustic radiation force impulse (ARFI), one of the point shear wave techniques, compared to conventional methods, assessing its effective role in diagnosis. Methods: This is a retrospective study; a total of 136 patients diagnosed with fatty liver disease through ultrasonography were enrolled. The anthropometric data of the patients were collected on the day of admission and blood tests, measurements of ARFI, and a point shear test were conducted using abdominal ultrasound; a biopsy was performed the following day. In addition, we calculated the aspartate aminotransferase-to-platelet ratio index (APRI) index based on four factors (FIB-4) and the NAFLD fibrosis score (NFS). Subsequently, we assessed the diagnostic accuracy of NITs within various subgroups based on the extent of obesity, steatosis, or NAFLD activity score. Results: ARFI has been shown to have the highest diagnostic value among various NITs, with AUROC values of 0.832, 0.794, 0.767, and 0.696 for ARFI, APRI, FIB-4, and NFS, respectively. In the morbidly obese subgroup, the AUROC values of ARFI, APRI, FIB-4, and NFS were 0.805, 0.769, 0.736, and 0.674. In the group with severe steatosis or non-alcoholic steatohepatitis (NASH), the AUROC values were 0.679, 0.596, 0.661, and 0.612, respectively, for severe steatosis and 0.789, 0.696, 0.751, and 0.691, respectively, for NASH. Conclusions: In conclusion, ARFI is not affected by various factors and maintains diagnostic accuracy compared to serum NITs. Therefore, we can recommend ARFI as a valuable diagnostic test to screen for advanced fibrosis in patients with NAFLD. [ABSTRACT FROM AUTHOR]

We investigate the quantum forces occurring between the defects and/or boundaries of a conformal field theory (CFT). We propose to model imperfect defects and boundaries as localized relevant double-trace operators that deform the CFT. Our focus is on pointlike and codimension-one planar defects. In the case of two parallel membranes, we point out that the CFT 2-point function tends to get confined and develops a tower of resonances with a constant decay rate when the operator dimension approaches the free field dimension. Using a functional formalism, we compute the quantum forces induced by the CFT between a variety of configurations of pointlike defects, infinite plates and membranes. Consistency arguments imply that these quantum forces are attractive at any distance. Forces of the Casimir–Polder type appear in the UV (ultraviolet), while forces of the Casimir type appear in the IR (infrared), in which case the CFT gets repelled from the defects. Most of the forces behave as a non-integer power of the separation, controlled by the dimension of the double-trace deformation. In the Casimir regime of the membrane–membrane configuration, the quantum pressure behaves universally as 1 / ℓ d ; however, information about the double-trace nature of the defects still remains encoded in the strength of the pressure. [ABSTRACT FROM AUTHOR]

High-intensity magnetic resonance-guided focused ultrasound (MRgFUS) is a non-invasive therapy to lesion brain tissue, used clinically in patients and pre-clinically in several animal models. Challenges with focused ablation in rodent brains can include skull and near-field heating and accurately targeting small and deep brain structures. We overcame these challenges by creating a novel method consisting of a craniectomy skull preparation, a high-frequency transducer (3 MHz) with a small ultrasound focal spot, a transducer positioning system with an added manual adjustment of ∼0.1 mm targeting accuracy, and MR acoustic radiation force imaging for confirmation of focal spot placement. The study consisted of two main parts. First, two skull preparation approaches were compared. A skull thinning approach (n = 7 lesions) was compared to a craniectomy approach (n = 22 lesions), which confirmed a craniectomy was necessary to decrease skull and near-field heating. Second, the two transducer positioning systems were compared with the fornix chosen as a subcortical ablation target. We evaluated the accuracy of targeting using histologic methods from a high-frequency transducer with a small ultrasound focal spot and MR acoustic radiation force imaging. Comparing a motorized adjustment system (∼1 mm precision, n = 17 lesions) to the motorized system with an added micromanipulator (∼0.1 mm precision, n = 14 lesions), we saw an increase in the accuracy of targeting the fornix by 133%. The described work allows for repeatable and accurate targeting of small and deep structures in the rodent brain, such as the fornix, enabling the investigation of neurological disorders in chronic disease models. [ABSTRACT FROM AUTHOR]

This study addresses the growing interest in developing new acoustophoresis designs for efficient particle separation, introducing a novel concave–convex electrode design for lymphocyte separation. Initially, a numerical model for acoustophoresis was employed and validated against existing experimental results in the literature with a 4% variance, based on the finite element method. Furthermore, in order to ensure the accuracy of the performed simulations, a mesh independency approach was employed for the piezoelectric substrate, alongside an investigation into resonant frequencies across the computational domain. These analyses were conducted to ensure that the results approximate experimental findings more closely and identify the frequency at which the maximum surface displacement occurs, making the results empirically reliable. As a major innovation, a new concentric concave–convex electrode design was introduced, and then the separation distance of targeted particles, as the goal parameter, was studied relative to the geometrical design and acoustofluidic operation parameters of the microfluidic chip. Through numerical analysis, the flow rate ranging from 7 to 14 μl/min and the applied radio frequency signal amplitude ranging from 16 to 26 V were investigated simultaneously. Results demonstrated the microfluidic chip's capability to function effectively across the entire range of voltage and flow rates examined. At the chip's highest operational point, with a flow rate of 13 μl/min and an applied radio frequency signal amplitude of 24 V, particle separation distance reached up to 380 μm. Under similar flow rates, cell conditions, and microchannel length, the particle separation distance has been improved by about 26% as compared with the standard electrode pattern, revealing a significant enhancement in separation efficiency and output purity. Moreover, due to the predominantly radial propagation of the acoustic waves and the expanding acoustic aperture, the resultant standing wave pattern spans a greater length of the microchannel. Assuming a constant injection velocity, this consequently extends the effective exposure time of particles to the acoustic radiation force, allowing for an increase in Stokes drag force. Given that drag force increases with velocity, it enables the opportunity to introduce higher input flow rates and throughput. [ABSTRACT FROM AUTHOR]

The analytical expression of the axial acoustic radiation force (ARF) exerted on a spherical particle located on the propagation axis of non-diffracting beams constructed by a discrete superposition of Bessel beams is calculated. The axial acoustic radiation for the fundamental non-diffracting beams as cosine beams, Bessel beams, Mathieu beams, and parabolic or Weber beams are considered. In this study, we extend the recent results on the ARF concerning Bessel and Mathieu beams to include the other fundamental non-diffracting cosine and parabolic beams. Additionally, the influences of each beam parameter, such as the angle of the half cone, the beam's own value, and the beam order on ARF will be examined, and the dependence of ARF with the particle radius is treated. In addition, some numerical simulations are performed the influence these parameters on the ARF. Furthermore, a comparison between the ARF exerted by each non-diffracting beam is accomplished by analyzing the curves of the dimensionless force function Yp versus the size parameter ka. This work may contribute to understanding the interaction between spherical particles and the fundamental non-diffracting beams. [ABSTRACT FROM AUTHOR]

Considering the lack of studies on the transient vibro-acoustic properties of conical shell structures, a Jacobi–Ritz boundary element method for forced vibro-acoustic behaviors of structure is proposed based on the Newmark-β integral method and the Kirchhoff time domain boundary integral equation. Based on the idea of the differential element method and the first-order shear deformation theory (FSDT), the vibro-acoustic model of conical shells is established. The axial and circumferential displacement tolerance functions are expressed using Jacobi polynomials and the Fourier series. The time domain response of the forced vibration of conical shells is calculated based on the Rayleigh–Ritz method and Newmark-β integral method. On this basis, the time domain response of radiated noise is solved based on the Kirchhoff integral equation, and the acoustic radiation characteristics of conical shells from forced vibration are analyzed. Compared with the coupled FEM/BEM method, the numerical results demonstrate the high accuracy and great reliability of this method. Furthermore, the semi-vertex angle, load characteristics, and boundary conditions related to the vibro-acoustic response of conical shells are examined. [ABSTRACT FROM AUTHOR]

Acoustic streaming enabled by a Lamb wave resonator (LWR) is efficient for particle trapping and enrichment in microfluidic channels. However, because Lamb waves combine the features of bulk acoustic waves and surface acoustic waves, the resulting acoustic streaming in the LWR occurs in multiple planes, and the particle flow behavior in this acoustofluidic system is largely unknown. Reported here are numerical simulations and laboratory experiments conducted to investigate the boundary conditions for particle motion inside a microvortex induced by an LWR. Upon dynamic capture, the particles' trajectories become orbital paths within an acoustic vortex. The suspended particles encounter two distinct acoustic phenomena, i.e., the drag force resulting from acoustic streaming and the acoustic radiation force, which exert forces in various directions on the particles. When the acoustic radiation force and the fluid drag force are dominant for large and small particles in a mixed solution, respectively, the large particles reside within the vortex while the small particles remain at its periphery. Conversely, when the acoustic radiation force is dominant for both types of particles, the distribution pattern is reversed. ARTICLE HIGHLIGHTS: HIGHLIGHTS • A Lamb wave resonator (LWR) was fabricated and used for particle manipulation, and upon analyzing the forces acting on the particles, two distinct distribution patterns are found. • Particles in the acoustic streaming are dominated by different forces depending on their size and the frequency of the LWR. • An LWR with a frequency of 370 MHz was used, and this frequency induces a potent acoustic flow in the LWR that is suitable for particle manipulation. [ABSTRACT FROM AUTHOR]

The acoustic radiation force (ARF) acting on particles measures the performance of microfluidic devices driven by standing surface acoustic waves (SSAWs). However, existing ARF calibration techniques rely on image post-processing or additional equipment. This work proposes a look-up table method to determine the ARF by examining the particle acoustophoresis mode in discrete phase-modulated SSAW fields, where the phase difference between the two counter-propagating SAWs is changed at fixed time intervals. Theoretical analysis indicates that particles in a straight channel migrate laterally either in the "locked" mode or the "drift" mode, while mode switching can be observed when the interval reaches a critical value highly dependent on the ARF amplitude. A look-up table can then be established for a given SSAW device. By observing the particle acoustophoresis modes at different phase-changing intervals, the ARF amplitude can be obtained from the easily determined critical interval. The procedure is demonstrated experimentally in an SSAW acoustofluidic device and compared with the particle tracking protocol to verify the former's effectiveness and demonstrate its operational simplicity. Inspired by the established theory, a method to improve the efficiency of particle acoustophoresis by optimizing the phase-modulating parameters is also proposed. [ABSTRACT FROM AUTHOR]

Abstract Superhydrophobic surfaces (SHS) exhibit a pronounced ability to resist wetting. When immersed in water, water does not penetrate between the microstructures of the SHS. Instead, a thin layer of trapped gas remains, i.e., plastron. This fractional wetting is also known as the Cassie–Baxter state (CB). Impairment of superhydrophobicity occurs when water penetrates the plastron and, when complete wetting is achieved, a Wenzel state (W) results. Subsequent recovery back to CB state is one of the main challenges in the field of SHS wetting. Current methods for plastron recovery require complex mechanical or chemical integration, are time‐consuming or lack spatial control. Here an on‐demand, contact‐less approach for performing facile transitions between these wetting states at micrometer length scales is proposed. This is achieved by the use of acoustic radiation force (ARF) produced by high‐intensity focused ultrasound (HIFU). Switching from CB to W state takes

In this study, we developed a novel type of dibenzocyclooctyne (DBCO)‐functionalized microbubbles (MBs) and validated their attachment to azide‐labelled sialoglycans on human pluripotent stem cells (hPSCs) generated by metabolic glycoengineering (MGE). This enabled the application of mechanical forces to sialoglycans on hPSCs through molecularly specific acoustic tweezing cytometry (mATC), that is, displacing sialoglycan‐anchored MBs using ultrasound (US). It was shown that subjected to the acoustic radiation forces of US pulses, sialoglycan‐anchored MBs exhibited significantly larger displacements and faster, more complete recovery after each pulse than integrin‐anchored MBs, indicating that sialoglycans are more stretchable and elastic than integrins on hPSCs in response to mechanical force. Furthermore, stimulating sialoglycans on hPSCs using mATC reduced stage‐specific embryonic antigen‐3 (SSEA‐3) and GD3 expression but not OCT4 and SOX2 nuclear localization. Conversely, stimulating integrins decreased OCT4 nuclear localization but not SSEA‐3 and GD3 expression, suggesting that mechanically stimulating sialoglycans and integrins initiated distinctive mechanoresponses during the early stages of hPSC differentiation. Taken together, these results demonstrated that MGE‐enabled mATC uncovered not only different mechanical properties of sialoglycans on hPSCs and integrins but also their different mechanoregulatory impacts on hPSC differentiation, validating MGE‐based mATC as a new, powerful tool for investigating the roles of glycans and other cell surface biomolecules in mechanotransduction. [ABSTRACT FROM AUTHOR]

Ultrasound directed self-assembly (DSA) utilizes the acoustic radiation force (ARF) associated with a standing ultrasound wave to organize particles dispersed in a fluid medium into specific patterns. The ARF is a superposition of the primary acoustic radiation force, which results from the incident standing ultrasound wave, and the acoustic interaction force, which originates from single and multiple scattering between neighboring particles. In contrast with most reports in the literature that neglect multiple scattering when calculating the ARF, we demonstrate that the deviation between considering single or multiple scattering may reach up to 100%, depending on the ultrasound DSA process parameters and material properties. We evaluate a theoretical case with three spherical particles in a viscous medium and derive operating maps that quantify the deviation between both scattering approaches as a function of the ultrasound DSA process parameters. Then, we study a realistic system with hundreds of particles dispersed in a viscous medium, and show that the deviation between the ARF resulting from single and multiple scattering increases with decreasing particle size and increasing medium viscosity, density ratio, compressibility ratio, and particle volume fraction. This work provides a quantitative basis for determining whether to consider single or multiple scattering in ultrasound DSA simulations. [ABSTRACT FROM AUTHOR]

Based on the solution of the problem of scattering of a plane harmonic wave by a rigid spherical body within a semi-infinite cylindrical cavity filled with a fluid, we have determined the radiation force acting on the body as a function of the primary wave frequency and the physical and geometrical parameters of the mechanical system. The total wave field potential has been expressed in a computationally convenient form. The numerical simulations have shown that the end wall of the cylindrical cavity significantly affects the radiation force acting on the spherical body at frequencies exceeding the first natural frequency of the radial oscillations of the fluid in the cylindrical cavity. As the gap between the boundaries of the body and the cavity decreases, at frequencies of the incident wave close to the "resonant" ones for the studied mechanical system, the radiation force increases significantly. The described methodology enables precise calculation of the radiation force and facilitates the determination of the incident wave frequency required for controlled motion and localization of a spherical body within a cylindrical cavity. [ABSTRACT FROM AUTHOR]

In general, it is difficult to visualize internal ocular structure and detect a lesion such as a cataract or glaucoma using the current ultrasound brightness-mode (B-mode) imaging. This is because the internal structure of the eye is rich in moisture, resulting in a lack of contrast between tissues in the B-mode image, and the penetration depth is low due to the attenuation of the ultrasound wave. In this study, the entire internal ocular structure of a bovine eye was visualized in an ex vivo environment using the compound acoustic radiation force impulse (CARFI) imaging scheme based on the phase-inverted ultrasound transducer (PIUT). In the proposed method, the aperture of the PIUT is divided into four sections, and the PIUT is driven by the out-of-phase input signal capable of generating split-focusing at the same time. Subsequently, the compound imaging technique was employed to increase signal-to-noise ratio (SNR) and to reduce displacement error. The experimental results demonstrated that the proposed technique could provide an acoustic radiation force impulse (ARFI) image of the bovine eye with a broader depth-of-field (DOF) and about 80% increased SNR compared to the conventional ARFI image obtained using the in-phase input signal. Therefore, the proposed technique can be one of the useful techniques capable of providing the image of the entire ocular structure to diagnose various eye diseases. [ABSTRACT FROM AUTHOR]

Ultrasonic treatment has been widely used in the mineral flotation process due to its advantages in terms of operational simplicity, no secondary pollutant formation, and safety. Currently, many studies have reported the effect of ultrasonic treatment on mineral flotation and shown excellent flotation performance. In this review, the ultrasonic mechanisms are classified into three types: the transient cavitation effect, stable cavitation effect, and acoustic radiation force effect. The effect of the main ultrasonic parameters, including ultrasonic power and ultrasonic frequency, on mineral flotation are discussed. This review highlights the uses of the application of ultrasonic treatment in minerals (such as the cleaning effect, ultrasonic corrosion, and desulfuration), flotation agents (such as dispersion and emulsification and change in properties and microstructure of pharmaceutical solution), and slurry (such formation of microbubbles and coalescence). Additionally, this review discusses the challenges and prospects of using ultrasonic approaches for mineral flotation. The findings demonstrate that the application of the ultrasonic effect yields diverse impacts on flotation, thereby enabling the regulation of flotation behavior through various treatment methods to enhance flotation indices and achieve the desired objectives. [ABSTRACT FROM AUTHOR]

Elastography can be used as a diagnostic method for quantitative characterization of tissue hardness information and thus, differential changes in pathophysiological states of tissues. In this study, we propose a new method for shear wave elastography (SWE) based on laser-excited shear wave, called photoacoustic shear wave elastography (PASWE), which combines photoacoustic (PA) technology with ultrafast ultrasound imaging. By using a focused laser to excite shear waves and ultrafast ultrasonic imaging for detection, high-frequency excitation of shear waves and noncontact elastic imaging can be realized. The laser can stimulate the tissue with the light absorption characteristic to produce the thermal expansion, thus producing the shear wave. The frequency of shear wave induced by laser is higher and the frequency band is wider. By tracking the propagation of shear wave, Young's modulus of tissue is reconstructed in the whole shear wave propagation region to further evaluate the elastic information of tissue. The feasibility of the method is verified by experiments. Compared with the experimental results of supersonic shear imaging (SSI), it is proved that the method can be used for quantitative elastic imaging of the phantoms. In addition, compared with the SSI method, this method can realize the noncontact excitation of the shear wave, and the frequency of the shear wave excited by the laser is higher than that of the acoustic radiation force (ARF), so the spatial resolution is higher. Compared to the traditional PA elastic imaging method, this method can obtain a larger imaging depth under the premise of ensuring the imaging resolution, and it has potential application value in the clinical diagnosis of diseases requiring noncontact quantitative elasticity. [ABSTRACT FROM AUTHOR]

In this paper, a horizontal-type surface acoustic wave device based on electrode width control interdigital transducer/polymethylmethacrylate/54° Y --X LiNbO3 is proposed to achieve the removal of non-specific binding particles by Rayleigh waves and the detection of meningococcus by Love waves. It is proved that the surface acoustic wave force plays a leading role in the removal of non-specific binding, and the frequency of the acoustic wave also affects the removal effect. The advantage of electrode width control interdigital transducer lies not only in overcoming the shortcomings of bidirectional and focused interdigital transducers, but also in improving the utilization of acoustic waves and the sensitivity of the sensor. When non-specific binding particles are removed by Rayleigh waves, the Love wave sensor shows a sensitivity of 330 Hz/(ng/l'L) and a detection limit of 50 pg/µL for meningococcus. [ABSTRACT FROM AUTHOR]

Due to the tunable characteristics of elastic waves, the vibroacoustic coupling behavior of a mechanical metastructure is a hot topic of underwater vehicles. In this work, a metastructure shell with active feedback control is presented and fabricated. The dynamic effective density and sound pressure level are derived to find the influences of acceleration and displacement feedback control. Different from a single cylinder, a double cylinder structure has both in-phase and anti-phase modes. Numerical results are obtained by Fourier transform and harmonic series expansion. With the introduction of an active feedback control system, the reduction of acoustic radiation shows low-frequency and broadband characteristics. In addition, finite element simulation is applied to support numerical results and present vibroacoustic characteristics. Finally, an experiment is performed in the anechoic chamber to illustrate the quiet metastructure shell, which can be applied to new designs of underwater vehicles. [ABSTRACT FROM AUTHOR]

Protein molecules adsorb on the air/liquid interface due to possessing a hydrophobic side. A full surface coverage is important in many processes such as in protein harvesting by foam fractionation. The adsorption of proteins in low concentration solutions is preceded by a relatively long time lag known as the induction period. This has been attributed to the formation of an adsorbed monolayer, which relies on the reorientation of the protein molecules. The reduction of the induction period can significantly facilitate the sorption process to reach full protein coverage. For this purpose acoustically induced non-spherical bubble oscillations can aid in the formation of the monolayer and enhance the sorption process. In this study, low frequency ultrasound was used to induce non-spherical oscillations on an air bubble attached to a capillary. Profile analysis tensiometry was deployed to examine the effect of these non-spherical oscillations on the sorption dynamics of different proteins. We observed that during the initial stages of adsorption, when the bubble surface is almost empty, non-spherical oscillations occur, which were found to significantly expedite the adsorption process. However, during later stages of the adsorption process, despite the continued presence of several sonication phenomena such as the primary radiation force and acoustic streaming, no change in adsorption behavior of the proteins could be noted. The occurrence, duration, and intensity of the non-spherical bubble oscillations appeared to be the sole contributing factors for the change of the sorption dynamics of proteins. [ABSTRACT FROM AUTHOR]

Background: Wilson's disease (WD) often leads to liver fibrosis and cirrhosis, and early diagnosis of WD cirrhosis is essential. Currently, there are few non-invasive prediction models for WD cirrhosis. The purpose of this study is to non-invasively predict the occurrence risk of compensated WD cirrhosis based on ultrasound imaging features and clinical characteristics. Methods: A retrospective analysis of the clinical characteristics and ultrasound examination data of 102 WD patients from November 2018 to November 2020 was conducted. According to the staging system for WD liver involvement, the patients were divided into a cirrhosis group (n = 43) and a non-cirrhosis group (n = 59). Multivariable logistic regression analysis was used to identify independent influencing factors for WD cirrhosis. A nomogram for predicting WD cirrhosis was constructed using R analysis software, and validation of the model's discrimination, calibration, and clinical applicability was completed. Due to the low incidence of WD and the small sample size, bootstrap internal sampling with 500 iterations was adopted for validation to prevent overfitting of the model. Results: Acoustic Radiation Force Impulse (ARFI), portal vein diameter (PVD), and serum albumin (ALB) are independent factors affecting WD cirrhosis. A nomogram for WD cirrhosis was constructed based on these factors. The area under the ROC curve (AUC) of the model's predictive ability is 0.927 (95% CI: 0.88–0.978). As demonstrated by 500 Bootstrap internal sampling validations, the model has high discrimination and calibration. Clinical decision curve analysis shows that the model has high clinical practical value. ROC curve analysis of the model's rationality indicates that the model's AUC is greater than the AUC of using ALB, ARFI, and PVD alone. Conclusion: The nomogram model constructed based on ARFI, PVD, and ALB can serve as a non-invasive tool to effectively predict the risk of developing WD cirrhosis. [ABSTRACT FROM AUTHOR]

Application of the reciprocity principle to evaluate the acoustic radiation from arbitrary multilayered fluid and solid materials is described. To include the effect of shear motion in surrounding media, including viscosity in a fluid, equations for the acoustic radiation from such materials under point force excitation are developed in terms of reflection and transmission coefficients for longitudinal and shear waves. Calculations for forcing on either side of the layered material and in arbitrary directions, and for any asymmetric layer arrangements, are conducted. The frequency range of the calculations is not restricted by thin-plate or thick-plate theory. The test case for the radiation from plates embedded in a viscous and attenuating fluid has been investigated in detail. The effect of viscosity and attenuation has been quantified and shown to be significant at high frequencies. Application to the problem of flow noise is also briefly discussed. [ABSTRACT FROM AUTHOR]

Acoustic radiation forced impulse (ARFI) is an integrated ultrasound method, measuring stiffness by point shear wave elastography. To evaluate the diagnostic performance of the ARFI of the liver and the spleen, combined with spleen dimension and platelet count, in predicting high-risk esophageal varices (HRVs) in cirrhotic patients, a prospective and cross-sectional study was conducted between February 2017 and February 2021. The following ratio scores were calculated based on ARFI measurements: ALSDP (ARFI Liver–Spleen Diameter-to-Platelet Ratio Score), ASSDP (ARFI Spleen–Spleen Diameter-to-Platelet Ratio Score), ASSAP (ARFI Spleen–Spleen Area-to-Platelet Ratio Score), and ALSAP (ARFI Liver–Spleen Area-to-Platelet Ratio Score). In 100 enrolled subjects, spleen ARFI, ASSDP, and ASSAP were significantly associated with HRVs in the prospective short- and long-term follow-ups and in the cross-sectional study (p < 0.05), while ALSDP and ALSAP were associated with HRVs only in the prospective long-term follow-up and cross-sectional study (p< 0.05). ASSAP was the best ARFI ratio score for HRVs at the long-term follow-up [value of area under curve (AUC) = 0.88], although all the ARFI ratio scores performed better than individual liver and spleen ARFI (AUC > 0.7). In our study, ARFI ratio scores can predict, in well-compensated cirrhotic patients, the risk of developing HVRs in short- and long-term periods. [ABSTRACT FROM AUTHOR]

The propagation of linear acoustic disturbances in an infinite, homogeneous, gray radiating plasma, initially in mechanical and radiation equilibrium, is considered. An exact governing equation for radiation acoustics in a radiating gray gas is derived, taking into account the influence of the transverse magnetic field. Radiation magnetohydrodynamics (MHD) is described by three hydrodynamic equations and two radiative momentum equations, making extensive use of the formalism of radiation thermodynamics. With the aim of more reliably describing the evolution of radiation magnetic–acoustic disturbance waves with scattering and attenuation, the conditions of radiation-thermal dissipation, the force of radiation resistance, as well as magnetic force and Joule heat are introduced into these equations. In this case, the Eddington approximation is used, which allows one to study the modes of radiation magnetohydrodynamic waves in two asymptotic cases—optically thin and thick gas. The exact control equation derived in the work made it possible, using the heuristic Whitham method, to obtain a set of approximate control equations of the lowest order, each of which is part of a reliable approximation to the exact equation in a certain region of the independent time variable. The relatively simple form of such equations made it possible to study the physical processes occurring in each radiation magnetic–acoustic wave without a formal solution to the full problem. [ABSTRACT FROM AUTHOR]

Gravity‐induced drainage is one of the main destabilizing mechanisms for soap bubbles and foams. Here we show that solely through acoustic levitation without introducing any chemical stabilizers, liquid drainage in the bubble film can be completely inhibited, therefore leading to a significant enhancement of bubble lifetime by more than two orders of magnitude and enabling the bubble to survive puncturing by a needle. Based on sound simulation and force analysis, it has been found that acoustic radiation force, exerted on both the inner and outer surfaces of the levitated bubble, acts in opposite directions, thus providing a squeezing effect to the bubble film. The hydrostatic pressure that induces drainage has been balanced by the acoustic radiation pressure exerted on both sides of the film, which is at the origin of the sound stabilization mechanism. This study provides new insights into the interplay between sound and soap bubbles or films, thus stimulating a wide range of fundamental research concerning bubble films and expanding their applications in bio/chemical reactors. [ABSTRACT FROM AUTHOR]

Cavitation noise, mainly from bubble cluster collapse, is a primary source of noise in underwater equipment. This study aims to elucidate the acoustic characteristics of bubble clusters with varying volume fractions (0.002%–43%), thereby illuminating the noise patterns generated during bubble cluster collapse. With the direct numerical simulation (DNS) method, we simulate the collapse of bubble clusters containing 352 vapor bubbles. Far-field sound pressure was calculated by using the Farassat 1 A integral solution of the Ffowcs Williams–Hawkings (FW–H) equation. The results show that when the volume fraction of bubble clusters is greater than 20%, the higher the volume fraction, the closer the far-field sound pressure radiated by its collapse is to that of the equivalent bubble (whose volume is equal to the volume of the bubble cluster), and when the volume fraction is greater than 30%, the sound pressure of the bubble cluster is basically the same as that of the equivalent bubble. Furthermore, when the volume fraction of bubble clusters is less than 1%, the lower the volume fraction is, the closer the sound pressure obtained through the superposition of a single bubble (each with an initial radius equal to that within clusters and in a free field) is to that of bubble clusters, and when the volume fraction is less than 0.1%, the bubble clusters' sound pressures agree with those obtained through superposition. This study reveals the far-field noise pattern generated by the collapse of bubble clusters, providing a theoretical basis for the prediction of cavitation noise. [ABSTRACT FROM AUTHOR]

In this research, clusters of 450 μm sized polystyrene particles, suspended in a solution of sodium dodecyl sulfate (SDS), were trapped at nodes of acoustic standing waves. It was observed for the first time that under certain circumstances, the clusters of polystyrene become unstable and start spontaneous ruptures. For further investigation of the mechanism of this phenomenon, the acoustic radiation forces between two polystyrene particles in an acoustic trap were studied using the finite element method (FEM), and it was demonstrated that when the particles go toward the trap and approach each other, after a specific distance, the repulsive acoustic interparticle force dramatically builds up. Finally, when it dominates the primary radiation force, it drives the particles apart. Observations show that by increasing the concentration of SDS in the solution from 0.25 to 3 g/l, the rate of spontaneous ruptures increases, and the clusters disintegrate at smaller radii. The threshold radius of the cluster decreased from 4.29 mm (on average) at 0.5 g/l to 2.8 mm (on average) at 3 g/l. FEM methods justified this observation too. As the concentration of SDS in the fluid increases, the viscosity of the fluid rises. Moreover, simulations indicated that by increasing the viscosity of the fluid, the value of repulsive interparticle force dramatically enhances, and the clusters fall apart more quickly. At the concentration of 1 g/l, however, an unexpected instability was observed in the clusters. Following studies on the zeta potential of the particles at different concentrations showed that this unexpected insatiability of the clusters at this concentration could be attributed to electrostatic repulsion among particles. Zeta potential of the particles in different SDS solutions showed that at this concentration, the net negative charge on the particles is maximum. So, the electrostatic repulsive force intensified the repulsive interparticle force among particles and made the cluster burst at the average radius of 2.63 mm. [ABSTRACT FROM AUTHOR]

Acoustic focusing of particle flow in microfluidics has been shown to be an efficient tool for particle separation for various chemical and biomedical applications. The mechanism behind the method is the selective effect of the acoustic radiation force on distinct particles. In this way, they can be selectively focused and separated. The technique can also be applied under stationary conditions, i.e., in the absence of fluid flows. In this study, the manipulation of self-propelled particles, such as Janus particles, in an acoustofluidic setup was investigated. In experiments with self-propelled Janus particles and passive beads, we explored the interplay between self-propulsion and the acoustic radiation force. Our results demonstrated unusual and potentially useful effects such as selective trapping, escape, and assisted escape in binary mixtures of active and passive particles. We also analyzed various aspects related to the behavior of Janus particles in acoustic traps in the presence and absence of flows. [ABSTRACT FROM AUTHOR]

Emulsion formulations should be monodispersed in terms of their stability. Therefore, there is a need for a device that can classify droplets of the desired size from polydispersed emulsions in a fluidized bed manufacturing system. In the previous study, we evaluated the fabrication of a droplet manipulation device using acoustic radiation forces through simulation using the finite element method. In this study, particle manipulation experiments using 1, 6, and 10 µm polystyrene particles were first estimated and evaluated in comparison with their theoretical particle behavior. Based on the results we obtained, the driving conditions and droplet behavior were derived, and the droplet manipulation device using ultrasonic waves to shrink monodisperse emulsions was evaluated. As a result, the droplet classification effect in the microchannel was confirmed to be consistent with the droplet behavior prediction, and the microchannel structure with a constriction component improved its classification effect. [ABSTRACT FROM AUTHOR]