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Background. The effects of 40% ethanolic nettle extract (the first factor) and adrenaline-induced stress (the second factor) were evaluated, both individually and in combination, on some biochemical and hematological parameters in rats using a two-factor analysis of variance. Materials and Methods. In experiments, we used 4 groups of rats. Animals of groups UD-nettle and UD+A received 40% ethanolic extract of nettle (Urtica dioica L.) in a dose of 5 mL/kg of body weight for 4 weeks. Rats of the groups (C-control) and (A-stress) received an appropriate amount of 40% ethanol. On the 29th day of the experiment, the stress was simulated in the animals of groups A and UD + A by an intramuscular injection of 0.1% adrenaline hydrochloride in the dose of 1 mg/kg of the body weight. The rats of the C and UD-nettle groups were injected with the appropriate amount of physiological solution. The animals were decapitated under anesthesia 24 hours after injections. Some hematological and biochemical parameters of the blood, liver, heart, and kidney of rats were studied. All experimental data were processed by ANOVA analysis. Results. Adrenaline stress has the most intense effect on the elevation of aspartate aminotransferase activity in blood, a decrease of superoxide dismutase activity in erythrocytes and concentration of reduced glutathione in the liver and heart, and increased levels of free radical reaction products in all studied organs of rats. The ANOVA analysis showed that nettle extract in the body of non-stressed animals has the most intense effect on reducing the content of lipid hydroperoxide in the liver and carbonyl groups of protein in the heart, and activates the glutathione chain of the antioxidant system in all organs. Administration of nettle extract to rats before stress induction inhibits lipid peroxidation in the kidneys and heart, decreases the concentration of carbonyl groups of protein in blood and liver, reduced glutathione concentration and the activity of glutathione peroxidase in erythrocytes. Our results indicate that the heart is more resistant to stress in terms of free radical formation, but is sensitive to the effect of nettle extract before stress induction in rats which is manifested by the increase in the activity of the enzymatic and non-enzymatic chain of the antioxidant system. All studied factors significantly affected the TBA-active products in the kidneys accompanied by increasing their concentration. The unaccounted factors affect the accumulation of lipid peroxidation products and catalase activity by more than 50%, while they have the least effect on the content of reduced glutathione in all rat tissues. Conclusion. The dispersion analysis confirmed the tissue specificity of the investigated factors’ individual influence and their combined effect on some biochemical and hematological parameters in the rats, as well as the protective and antioxidant properties of nettle extract.

Introduction. Psychological stress is an important factor in the pathogenesis of the periodontal disease. It is therefore essential to determine the relationship between psychosomatic stress and periodontal disease. For the treatment of stress in patients with generalized periodontitis, a medicinal composition is proposed, for determining its antidisbiotic effect in the experimental substantiation of its periodontio-protective action. Aim. to determine the psychosomatic state of subjects, the influence of psychological stress on the periodontal tissue and the experimental substantiation of the antidisbiotic effect of drug composition for stress neutralization. Materials and methods. To study the subjective human responses to the effect of various environmental factors, a specially designed questionnaire is advisable to be used. Diagnosis of the anxiety level was made by a self-determination test including reactive and personal anxiety according to Spielberger. Adrenaline stress was followed on rats, by oral application of a gel with adrenalin in a 0.36 mg/kg dose, for 10 days. Lincomycin was introduced with drinking water in a dose of 60 mg/kg. The gel of adrenoblockers (zocson + nicergolin and sibason) was applied in a 0.6 mg/kg does. The activity of urease, lysozyme, elastase was determined in serum, and the degree of disbiosis was calculated according to Levitsky. Results and disscusions. The Spielberger test showed a moderate level of reactive anxiety among respondents - 34.72±2.45, and a high level of personal anxiety - 50.64±3.58. In patients with a high level of personal anxiety, a significantly higher prevalence of periodontal disease, especially generalized periodontitis, has been detected. Experimental studies have shown that gel adrenoblockers decrease urease activity, increase lysozyme levels, which leads to a significant reduction of disbiosis. Conclusions. A high level of personal anxiety - 50.64±3.58 - leads to a significant increase in the prevalence of periodontal diseases -- 95.09±6.7%, especially generalized periodontitis -- 88.72±6.5%. Oral application of a gel containing adrenoblockers had an antidisbiotic effect after modeling of the adrenaline stress by administration of adrenalin and lincomycin to animals. [ABSTRACT FROM AUTHOR]