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In this paper, a discrete air model (DAM) is developed to capture the discontinuous characteristics of air at different locations during the rapid filling process in long-range, large-scale water pipeline. By introducing the continuity and momentum equations of air and combining them with the water control equation and the interface continuity equation, an improved model based on the uniform air is derived. The accuracy of the model is verified by comparing it with experimental data and the results of the original uniform air model (UAM). Subsequently, a long-range, large-scale pipeline was considered to investigate the dynamic properties of air in large systems, which had not been covered in previous studies. Additionally, the influence of air dynamic characteristics on initial air volume affected by different air lengths and various pipe diameters in large systems – is further studied. Results show that an increased pipe diameter expands the contact area of the air–water interface, often resulting in the UAM underestimating the maximum peak pressure. The propagation process of transient waves in air is divided into three stages: propagation stage with multiple variation, maximum value stage with interface propulsive, and stability stage with several fluctuations, which corresponds to the pressure fluctuation curve. This explains the occurrence of small fluctuations and peaks in the curve. Therefore, the peak pressure simulated by the proposed DAM offers a better understanding of wave behaviours. [ABSTRACT FROM AUTHOR]

Humic-like substances (HULIS), a class of macromolecular photosensitizers, are major components of light-absorbing aerosols in the atmosphere. Due to the amphiphilic nature of HULIS, they tend to appear at the air-water interface of atmospheric water. However, there have been limited studies on the photosensitized chemistry of HULIS at the air-water interface owing to the lack of methods with high interface selectivity. Here, a unique field-induced droplet ionization mass spectrometry (FIDI-MS) technique was used to investigate the photosensitized chemistry of several samples at the air-water interface. These samples include commercially available humic acid and the PM2.5 sample collected during Beijing's severe winter haze in December 2016. Ultrahigh-resolution Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) was also applied to analyze the atomic compositions, the degree of unsaturation and the aromaticity of these samples. Both Type I and Type II photosensitized oxidation pathways were observed, which were postulated to be dictated by the packing density of the molecules at the air-water interface. These findings reveal that the photosensitization of HULIS at the air-water interface contributes greatly to the formation of atmospheric aerosols, further advancing our understanding of the important roles played by photosensitized chemistry in the atmosphere. [ABSTRACT FROM AUTHOR]

At the air–water interface, many reactions are accelerated, sometimes by several orders of magnitude. This phenomenon has proved to be particularly important in water microdroplets, where the spontaneous oxidation of many species stable in bulk has been experimentally demonstrated. Different theories have been proposed to explain this finding, but it is currently believed that the role of interfacial electric fields is key. In this work, we have carried out a quantum chemistry study aimed at shedding some light on this question. We have studied two prototypical processes in which a hydroxide anion transfers its excess electron to either the water environment or a dioxygen molecule. To model the interface, we use a cluster of 21 water molecules immersed in an electric field, and we examine the energetics of the studied reactions as a function of field magnitude. Our results reveal that electric fields close to those estimated for the neat air–water interface (∼0.15 V ⋅ Å−1) have a moderate effect on the reaction energetics and that much stronger fields (>1 V ⋅ Å−1) are required to get spontaneous electron transfer. Therefore, the study suggests that additional factors such as an excess charge in microdroplets need to be considered for explaining the experimental observations. [ABSTRACT FROM AUTHOR]

Water microdroplets have been demonstrated to exhibit extraordinary chemical behaviors, including the abilities to accelerate chemical reactions by several orders of magnitude and to trigger reactions that cannot occur in bulk water. One of the most striking examples is the spontaneous generation of hydroxyl radical from hydroxide ions. Alcohols and alkoxide ions, being structurally similar to water and hydroxide ions, might exhibit similar behavior on microdroplets. Here, we report the spontaneous generation of alkoxide radicals from alcohols (RCH2OH) in aqueous microdroplets through quantum chemical calculations, quantum mechanical/molecular mechanical (QM/MM) molecular dynamics (MD) simulations, ab initio MD simulations, and mass spectrometry. Our results show that an electric field (EF) on the order of 10−1 V/Å and partial solvation at the air‐water interface jointly promote the dissociation of RCH2OH into RCH2O− and H3O+ ions. QM/MM MD simulations indicate that RCH2O− can be ionized to produce RCH2O⋅ radicals on the microdroplet surface. Furthermore, partial solvation and the EF collaboratively catalyze the isomerization of the RCH2O⋅ radical into a more stable tautomer, R⋅CHOH. This study highlights the molecular mechanisms underlying the widespread generation of radicals at the microdroplet surface and provides insights into the importance of fundamental alcohol chemistry in the atmosphere. [ABSTRACT FROM AUTHOR]

• The quantum chemical calculations and ab initio dynamics simulation were adopted to clarify the gas phase and air-water interface reaction mechanisms of CH 3 CHOO and H 2 SO 4. • The reaction of CH 3 CHOO with H 2 SO 4 plays an active role in the CH 3 CHOO removal channel. • The formed interfacial CH 3 HC(OOH)OSO 3 − and H 3 O+ ions can attract candidate species for particle formation. • The oligomerization reaction of CH 3 CHOO with HPES in the gas phase is both thermochemically and kinetically favored • The reaction product can form stable clusters with sulfuric acids, ammonias and water molecules. The formation of oligomeric hydrogen peroxide triggered by Criegee intermediate maybe contributes significantly to the formation and growth of secondary organic aerosol (SOA). However, to date, the reactivity of C2 Criegee intermediates (CH 3 CHOO) in areas contaminated with acidic gas remains poorly understood. Herein, high-level quantum chemical calculations and Born-Oppenheimer molecular dynamics (BOMD) simulations are used to explore the reaction of CH 3 CHOO and H 2 SO 4 both in the gas phase and at the air-water interface. In the gas phase, the addition reaction of CH 3 CHOO with H 2 SO 4 to generate CH 3 HC(OOH)OSO 3 H (HPES) is near-barrierless, regardless of the presence of water molecules. BOMD simulations show that the reaction at the air-water interface is even faster than that in the gas phase. Further calculations reveal that the HPES has a tendency to aggregate with sulfuric acids, ammonias, and water molecules to form stable clusters, meanwhile the oligomerization reaction of CH 3 CHOO with HPES in the gas phase is both thermochemically and kinetically favored. Also, it is noted that the interfacial HPES− ion can attract H 2 SO 4 , NH 3 , (COOH) 2 and HNO 3 for particle formation from the gas phase to the water surface. Thus, the results of this work not only elucidate the high atmospheric reactivity of C2 Criegee intermediates in polluted regions, but also deepen our understanding of the formation process of atmospheric SOA induced by Criegee intermediates. [Display omitted] [ABSTRACT FROM AUTHOR]

[Display omitted] • MD simulations reveal the interfacial properties of PFBA, GenX, and PFOA. • Molecular insight into PFAS self-assembly and their air-water interfacial structure. • PFAS molecular structure affect their orientation and mobility at the interface. • Long chain PFOA diffuses slower than GenX and PFBA at the air-water interface. • Surface tension of PFAS are lower than their hydrocarbon analogs. • Short chain PFAS need less surface coverage than long chain to reduce surface tension. Understanding the intricate molecular-level details of toxic per- and polyfluoroalkyl substances (PFAS) partitioning to the air–water interface holds paramount importance in evaluating their fate and transport, as well as for finding safer alternatives for various applications, including aqueous film forming foams. The behavior of these substances at interfaces strongly depends on molecular architecture, chemistry, and concentration, which define molecular packing, self-assembly, interfacial diffusion, and the surface tension. Modeling of three PFAS surfactants, namely, longer-tail (perfluorooctanoate (PFOA)) and shorter-tail (perfluorobutanoate (PFBA) and 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy) propanoate (GenX)) has been conducted using atomistic molecular dynamics simulations. A systematic comparison between these representative PFAS of different sizes and structure reveals factors influencing their association behavior, mechanism of surface tension reduction, and interfacial mobility as a function of surface coverage. Shorter-chain PFAS surfactants (GenX or PFBA) require lower surface coverage compared to longer chain (PFOA) PFAS to achieve the same decrease in surface tension. However, a higher concentration of GenX and PFBA is necessary in the bulk aqueous solution to achieve the same surface coverage as PFOA, due to their higher solubility in water. The PFAS molecular orientation and mobility at the interface are found to be vastly influenced by the length and architecture of the hydrophobic fluorocarbon tail. A significant ordering of the water dipole moment near the anionic headgroup is apparent at high surface concentration. A direct correlation is established between the PFAS interfacial properties and PFAS-PFAS, PFAS-counterion, and PFAS-water interactions. [ABSTRACT FROM AUTHOR]

The efficient reduction of CO2 into valuable products is a challenging task in an international context marked by the climate change crisis and the need to move away from fossil fuels. Recently, the use of water microdroplets has emerged as an interesting reaction media where many redox processes which do not occur in conventional solutions take place spontaneously. Indeed, several experimental studies in microdroplets have already been devoted to study the reduction of CO2 with promising results. The increased reactivity in microdroplets is thought to be linked to unique electrostatic solvation effects at the air‐water interface. In the present work, we report a theoretical investigation on this issue for CO2 using first‐principles molecular dynamics simulations. We show that CO2 is stabilized at the interface, where it can accumulate, and that compared to bulk water solution, its electron capture ability is larger. Our results suggest that reduction of CO2 might be easier in interface‐rich systems such as water microdroplets, which is in line with early experimental data and indicate directions for future laboratory studies. The effect of other relevant factors which could play a role in CO2 reduction potential is discussed. [ABSTRACT FROM AUTHOR]

We present a catalyst‐free route for the reduction of carbon dioxide integrated with the formation of a carbon‐carbon bond at the air/water interface of negatively charged aqueous microdroplets, at ambient temperature. The reactions proceed through carbanion generation at the α‐carbon of a ketone followed by nucleophilic addition to CO2. Online mass spectrometry reveals that the product is an α‐ketoacid. Several factors, such as the concentration of the reagents, pressure of CO2 gas, and distance traveled by the droplets, control the kinetics of the reaction. Theoretical calculations suggest that water in the microdroplets facilitates this unusual chemistry. Furthermore, such a microdroplet strategy has been extended to seven different ketones. This work demonstrates a green pathway for the reduction of CO2 to useful carboxylated organic products. [ABSTRACT FROM AUTHOR]

In this paper, a discrete air model (DAM) is developed to capture the discontinuous characteristics of air at different locations during the rapid filling process in long-range, large-scale water pipeline. By introducing the continuity and momentum equations of air and combining them with the water control equation and the interface continuity equation, an improved model based on the uniform air is derived. The accuracy of the model is verified by comparing it with experimental data and the results of the original uniform air model (UAM). Subsequently, a long-range, large-scale pipeline was considered to investigate the dynamic properties of air in large systems, which had not been covered in previous studies. Additionally, the influence of air dynamic characteristics on initial air volume affected by different air lengths and various pipe diameters in large systems – is further studied. Results show that an increased pipe diameter expands the contact area of the air–water interface, often resulting in the UAM underestimating the maximum peak pressure. The propagation process of transient waves in air is divided into three stages: propagation stage with multiple variation, maximum value stage with interface propulsive, and stability stage with several fluctuations, which corresponds to the pressure fluctuation curve. This explains the occurrence of small fluctuations and peaks in the curve. Therefore, the peak pressure simulated by the proposed DAM offers a better understanding of wave behaviours.

ABSTRACTMany artificial molecular machines have been synthesized, and various functions have been expressed by changing their molecular conformations. However, their structures are still simple compared with those of biomolecular machines, and more energy is required to control them. To design artificial molecular machines with more complex structures and higher functionality, it is necessary to combine molecular machines with simple movements such as components. This means that the motion of individual molecular machines must be precisely controlled and observed in various environments. At the air – water interface, the molecular orientation and conformation can be controlled with little energy as thermal fluctuations. We designed various molecular machines and controlled them using mechanical stimuli at the air – water interface. We also controlled the transfer of forces to the molecular machines in various lipid matrices. In this review, we describe molecular pliers with amphiphilic binaphthyl, molecular paddles with binuclear platinum complexes, and molecular rotors with julolidine and BODIPY that exhibit twisted intramolecular charge transfer.

This study explores how the unsaturation patterns of three acetogenins from Porcelia macrocarpa (2S,3R,4R)-3-hydroxy-4-methyl-2-(eicos-11′-yn-19′-enyl)-butanolide (1), (2S,3R,4R)-3-hydroxy-4-methyl-2-(eicos-11′-ynyl)-butanolide (2), and (2S,3R,4R)-3-hydroxy-4-methyl-2-eicosyl-butanolide (3)—affect their interactions with lipid interfaces, using Langmuir monolayers of dipalmitoyl-phosphoethanolamine (DPPE) as a model for protozoal membranes. The hypothesis posits that unsaturated acetogenins (1 and 2) will exhibit distinct behaviors compared to the saturated acetogenin (3) due to structural differences. Experiments incorporated each acetogenin into DPPE monolayers, employing techniques like tensiometry, dilatational rheology, infrared spectroscopy, and Brewster angle microscopy (BAM) to assess interactions and changes in film properties. Results showed that unsaturated acetogenin 1, active against Leishmania infantum and Trypanosoma cruzi, destabilized the film and induced rapid molecular reorganization, while acetogenin 2, inactive against these parasites, uniquely increased the viscous contribution to viscoelasticity. Saturated acetogenin 3 did not integrate into the film. All acetogenins enhanced fluidity, reducing elasticity and viscosity, with BAM revealing phase separation only in acetogenin 2. Infrared spectroscopy underscored the impact of drug-lipid interactions, leading to the conclusion that unsaturated acetogenins 1 and 2 have a more significant influence on thermodynamic and structural properties than acetogenin 3, offering insights into their potential therapeutic mechanisms.

Hydroxyl radical (OH) is a key oxidant that triggers atmospheric oxidation chemistry in both gas and aqueous phases. The current understanding of its aqueous sources is mainly based on known bulk (photo)chemical processes, uptake from gaseous OH, or related to interfacial O3 and NO3 radical-driven chemistry. Here, we present experimental evidence that OH radicals are spontaneously produced at the air-water interface of aqueous droplets in the dark and the absence of known precursors, possibly due to the strong electric field that forms at such interfaces. The measured OH production rates in atmospherically relevant droplets are comparable to or significantly higher than those from known aqueous bulk sources, especially in the dark. As aqueous droplets are ubiquitous in the troposphere, this interfacial source of OH radicals should significantly impact atmospheric multiphase oxidation chemistry, with substantial implications on air quality, climate, and health.

Amphiphilic silk fibroin (SF) forms stable adsorption layers at the air–water interface. The range of the investigated protein concentrations can be divided into two parts according to the peculiarities of the surface layer properties. At protein concentrations from 0.0005 to 0.01 mg/mL, the dynamic surface elasticity monotonically increases with the concentration and surface age and reaches values of up to 220 mN/m. In this range, the adsorption layer compression leads to a fast increase of the surface pressure. In the second part (>0.01 mg/mL), the surface elasticity decreases again and the kinetic dependences of the film thickness and adsorbed amount change only a little. In this case, the layer compression leads only to a slight increase of the surface pressure. These two types of behavior can be attributed to the distinctions in the protein aggregation in the surface layer. Atomic force microscopy (AFM) investigations of the layers transferred from the liquid surface onto a mica surface by the Langmuir–Schaefer method show some peculiarities of the layer morphology in the intermediate concentration range (~0.02 mg/mL). [ABSTRACT FROM AUTHOR]

Highly transparent, conductive nanosheets are extremely attractive for advanced opto-electronic applications. Previously, we have demonstrated that transparent, conductive Au nanosheets can be prepared by UV irradiation of Au nanoparticle (AuNP) monolayers spread on water, which serves as the subphase. However, thick Au nanosheets cannot be fabricated because the method is not applicable to large Au NPs. Further, in order to fabricate nanosheets with different thicknesses and compositions, it is necessary to prepare the appropriate NPs. A strategy is needed to produce nanosheets with different thicknesses and compositions from a single type of metal NP monolayer. In this study, we show that this UV irradiation technique can easily be extended as a nanosheet modification method by using subphases containing metal ions. UV irradiation of 4.7 nm AuNP monolayers on 480 µM HAuCl4 solution increased the thickness of Au nanosheets from 3.5 nm to 36.5 nm, which improved conductivity, but reduced transparency. On the other hand, the use of aqueous AgNO3 and CH3COOAg solutions yielded Au-Ag hybrid nanosheets; however, their morphologies depended on the electrolytes used. In Au-Ag nanosheets prepared on aqueous 500 µM AgNO3, Au and Ag metals are homogeneously distributed throughout the nanosheet. On the other hand, in Au-Ag nanosheets prepared on aqueous 500 µM CH3COOAg, AuNPs still remained and these AuNPs were covered with a Ag nanosheet. Further, these Au-Ag hybrid nanosheets had high conductivity without reduced transparency. Therefore, this UV irradiation method, modified by adding metal ions, is quite effective at improving and diversifying properties of Au nanosheets. [ABSTRACT FROM AUTHOR]

Environmental media affect the atmospheric oxidation processes of phenolic compounds (PhCs) released from biomass burning in the troposphere. Phenol (Ph), 4-hydroxybenzaldehyde (4-HBA), and vanillin (VL) are chosen as model compounds to investigate their reaction mechanism and kinetics at the air-water (A-W) interface, on TiO2 clusters, in the gas phase, and in bulk water using a combination of molecular dynamics simulation and quantum chemical calculations. Of them, Ph was the most reactive one. The occurrence percentages of Ph, 4-HBA, and VL staying at the A-W interface are ~72 %, ~68 %, and ~73 %, respectively. As the size of (TiO2)n clusters increases, the adsorption capacity decreases until n > 4, and beyond this, the capacity remains stable. A-W interface and TiO2 clusters facilitate Ph and VL reactions initiated by the O3 and HO•, respectively. However, oxidation reactions of 4-HBA are little affected by environmental media because of its electron-withdrawing group. The O3- and HO•-initiated reaction rate constant (k) values follow the order of PhA-W > VLTiO2 > VLA-W > 4-HBAA-W > 4-HBATiO2 > PhTiO2 and VLTiO2 > PhA-W > VLA-W > 4-HBATiO2 > PhTiO2 > 4-HBAA-W, respectively. Some byproducts are more harmful than their parent compounds, so should be given special attention. This work provides key evidence for the rapid oxidation observed in the O3/HO• + PhCs experiments at the A-W interface. More importantly, differences in oxidation of PhCs by different environmental media due to the impact of substituent groups were also identified. [ABSTRACT FROM AUTHOR]

While many aspects of single-particle electron cryo-microscopy (cryo-EM) of biological macromolecules have reached a sophisticated level of development, this is not yet the case when it comes to preparing thin samples on specimen grids. As a result, there currently is considerable interest in achieving better control of both the sample thickness and the amount of area that is useful, but this is only one aspect in which improvement is needed. This Perspective addresses the further need to prevent the macromolecular particles from making contact with the air-water interface, something that can result in preferential orientation and even structural disruption of macromolecular particles. This unwanted contact can occur either as the result of free diffusion of particles during the interval between application, thinning and vitrification of the remaining buffer, or-when particles have been immobilized-by the film of buffer becoming too thin prior to vitrification. An opportunity now exists to apply theoretical and practical insights from the fields of thin-film physical chemistry and interfacial science, in an effort to bring cryo-EM sample preparation to a level of sophistication that is comparable to that of current data collection and analysis.

The motion of magnetic particles under magnetic fields is an object to be solved in association with basic and practical phenomena. Movement phenomena of magnetite-encapsulated graphene particles at air–water interfaces were evaluated by manufacturing a feedback control system of the magnetic field to cause the motion of particles due to magnetic torque. A homogeneous magnetic field was generated using two pairs of electromagnets located perpendicular to each other, which were connected to an electronic switch. The system influenced the translational movement and the self-rotational speed of magnetic particles located at a center on the surface of fluid media in a continuous duty cycle. Operating the particle at a remote control in the same duty cycle at the air–water surface, the short and elongated magnetic particles successfully rotated. In addition, the rotational speed of the curved particle was slower than that of the elongated particle. The results indicate that the translational and self-rotational movements of magnetite-encapsulated graphene particles at the air–water interface under the external magnetic field are size- and shape-dependent for the speed and the direction. A short magnetic particle was used as a target particle to rotate on cancer cell lines, aiming to study the advantage of this method to induce the growth of HeLa cells. It was monitored for up to 4 days with and without magnetic particles by checking the viability and morphology of cells before and after the electromagnetic treatment. As an outcome, the movement of magnetic particles reduced the number of biological cells, at least on HeLa cells, but it was inactive on the viability of HeLa cells. [ABSTRACT FROM AUTHOR]

Although single-particle cryogenic electron microscopy (cryo-EM) has been applied extensively for elucidating many crucial biological mechanisms at the molecular level, this technique still faces critical challenges, the major one of which is to prepare the high-quality cryo-EM specimen. Aiming to achieve a more reproducible and efficient cryo-EM specimen preparation, novel supporting films including graphene-based two-dimensional materials have been explored in recent years. Here we report a robust and simple method to fabricate EM grids coated with single- or few-layer reduced graphene oxide (RGO) membrane in large batch for high-resolution cryo-EM structural determination. The RGO membrane has decreased interlayer space and enhanced electrical conductivity in comparison to regular graphene oxide (GO) membrane. Moreover, we found that the RGO supporting film exhibited nice particle-absorption ability, thus avoiding the air-water interface problem. More importantly, we found that the RGO supporting film is particularly useful in cryo-EM reconstruction of sub-100-kDa biomolecules at near-atomic resolution, as exemplified by the study of RBD-ACE2 complex and other small protein molecules. We envision that the RGO membranes can be used as a robust graphene-based supporting film in cryo-EM specimen preparation.

Amphiphilic silk fibroin (SF) forms stable adsorption layers at the air–water interface. The range of the investigated protein concentrations can be divided into two parts according to the peculiarities of the surface layer properties. At protein concentrations from 0.0005 to 0.01 mg/mL, the dynamic surface elasticity monotonically increases with the concentration and surface age and reaches values of up to 220 mN/m. In this range, the adsorption layer compression leads to a fast increase of the surface pressure. In the second part (>0.01 mg/mL), the surface elasticity decreases again and the kinetic dependences of the film thickness and adsorbed amount change only a little. In this case, the layer compression leads only to a slight increase of the surface pressure. These two types of behavior can be attributed to the distinctions in the protein aggregation in the surface layer. Atomic force microscopy (AFM) investigations of the layers transferred from the liquid surface onto a mica surface by the Langmuir–Schaefer method show some peculiarities of the layer morphology in the intermediate concentration range (~0.02 mg/mL).

The fix-grid method of characteristic (MOC) has been the main numerical scheme for modeling the transient pipe flows with an entrapped air pocket, where the Courant number Cr usually equals one (i.e., Cr=1) to ensure its accuracy and stability. However, Cr=1 cannot always be guaranteed in each pipe of real pipe systems; thus, the MOC needs to be approximated by interpolation or wavespeed adjustment. This could lead to large accumulated numerical errors and serious shape distortion of simulated pressure curves. To address this problem, an alternative coupled scheme, which combines the second-order Godunov-type scheme (GTS) and the MOC, is developed. Specifically, the conservation equations with unsteady friction of the water column are numerically solved by the GTS, and the moving air-water interface is modeled and captured by the coupled GTS-MOC scheme. The simulated pressure curves by the GTS-MOC scheme are compared with both MOC results and laboratory experiments. The proposed scheme with unsteady friction can better reproduce the experimental pressure oscillations, and is more robust and efficient than the MOC. The MOC scheme with Cr<1 and coarse grids causes more obvious numerical dissipation during an intensive transient induced by relatively high inlet pressure, in which more high-frequency waves occur. [ABSTRACT FROM AUTHOR]

Hydrophobins are small amphiphilic proteins that are conserved in filamentous fungi. They localized on the conidial surface to make it hydrophobic, which contributes to conidial dispersal in the air, and helps fungi to infect plants and mammals and degrade polymers. Hydrophobins self-assemble and undergo structural transition from the amorphous state to the rodlet (rod-like multimeric structure) state. However, it remains unclear whether the amorphous or rodlet state is biologically functional and what external factors regulate state transition. In this study, we analyzed the self-assembly of hydrophobin RolA of Aspergillus oryzae in detail and identified factors regulating this process. Using atomic force microscopy, we observed RolA rodlet formation over time, and determined “rodlet elongation rate” and “rodlet formation frequency.” Changes in these kinetic parameters in response to pH and salt concentration suggest that RolA rodlet formation is regulated by the strength of ionic interactions between RolA molecules. [ABSTRACT FROM AUTHOR]

Betaine is a kind of zwitterionic surfactant with both positive and negative charge groups on the polar head, showing good surface activity and aggregation behaviors. The interfacial adsorption, structures and properties of n-dodecyl betaine (NDB) at different surface coverages at the air–water interface are studied through molecular dynamics (MD) simulations. Interactions between the polar heads and water molecules, the distribution of water molecules around polar heads, the tilt angle of the NDB molecule, polar head and tail chain with respect to the surface normal, the conformations and lengths of the tail chain, and the interfacial thickness of the NDB monolayer are analyzed. The change of surface coverage hardly affects the locations and spatial distributions of the water molecules around the polar heads. As more NDB molecules are adsorbed at the air–water interface, the number of hydrogen bonds between polar heads and water molecules slightly decreases, while the lifetimes of hydrogen bonds become larger. With the increase in surface coverage, less gauche defects along the alkyl chain and longer NDB chain are obtained. The thickness of the NDB monolayer also increases. At large surface coverages, tilted angles of the polar head, tail chain and whole NDB molecule show little change with the increase in surface area. Surface coverages can change the tendency of polar heads and the tail chain for the surface normal. [ABSTRACT FROM AUTHOR]

Cryo-EM samples prepared using traditional methods often suffer from too few particles, poor particle distribution, strongly biased orientation, or damage from the air-water interface. Here we report that functionalization of graphene oxide (GO) coated grids with amino groups concentrates samples on the grid with improved distribution and orientation. By introducing a PEG spacer, particles are kept away from both the GO surface and the air-water interface, protecting them from potential denaturation.

Pore‐network models are powerful tools for studying particle transport in complex porous media, and investigating the role of interfaces in their fate. The first step in simulating particle transport using pore‐network models is to quantitatively describe particle transport in a single pore, and obtain relationships between pore‐averaged deposition rate coefficients and various pore‐scale parameters. So, in this study, a three‐dimensional (3D) mathematical model is developed to simulate the transport and retention of nanoparticles within a single partially saturated pore with an angular cross‐section. The model accounts for particle deposition at solid‐water interfaces (SWIs), air‐water interfaces (AWIs), and air‐water‐solid (AWS) contact regions. We provide a novel formulation for particle diffusive transport from AWI to AWS, where particles are assumed to be retained irreversibly by capillary forces. The model involves 12 dimensionless parameters representing various physicochemical conditions. The 3D model results are averaged over the pore cross‐section and then fitted to breakthrough curves from one‐dimensional (1D) advection‐dispersion‐sorption equations with three‐site kinetics to estimate 1D‐averaged deposition rate coefficients at interfaces. We find that half‐corner angle, particle size, radius of curvature of AWI, and mean flow velocity have a significant effect on those coefficients. In contrast, chemical parameters such as ionic strength and surface potentials of particles and interfaces have negligible effects. AWS is found to be the major retention site for particles, especially for hydrophobic particles. We develop algebraic relationships between 1D‐averaged deposition rate coefficients at interfaces vis‐à‐vis various pore‐scale parameters. These relationships are needed for pore‐network models to upscale nanoparticle transport to continuum scale. Key Points: A novel formulation for particle diffusive transport from air‐water interface to contact regionParameters describing geometry, flow, and hydrophobic energy govern particle retention at interfacesDeveloped algebraic relationships between 1D‐averaged deposition rate coefficients at interfaces vis‐à‐vis various pore‐scale parameters [ABSTRACT FROM AUTHOR]

Polysorbates (PS) are nonionic surfactants that are commonly included in protein formulations to mitigate the formation of interfacial stress-induced protein particles and thus increase their long-term storage stability. Nonetheless, factors that dictate the efficiency of different polysorbates in mitigating protein particle formation, especially during the application of interfacial stresses, are often ill defined. Here, we used a Langmuir trough to determine the surface activity of two IgG1 monoclonal antibodies formulated with two different polysorbates (PS20 and PS80) when subjected to interfacial dilatational stress. Interfacial properties of these formulations were then correlated with characterization of subvisible protein particles measured by micro-flow imaging (MFI). Both mAbs, when formulated in PS20, demonstrate faster adsorption kinetics and higher surface activity compared to PS80 or surfactant-free formulations. Compression/expansion results suggest that when exposed to interfacial dilatational stresses, both mAb/PS20 formulations display interfacial properties of PS20 alone. In contrast, interfacial properties of both mAb/PS80 formulations suggest mAbs and PS80 are co-adsorbed to the air–water interface. Further, MFI analysis of the interface and the bulk solution confirms that PS20 is more effective than PS80 at mitigating the formation of larger particles in the bulk solution in both mAbs. Concomitantly, the efficiency of PS to prevent interface-induced protein particle formation also depended on the protein's inherent tendency to aggregate at a surfactant-free interface. Together, the studies presented here highlight the importance of determining the interfacial properties of mAbs, surfactants, and their combinations to make informed formulation decisions about the choice of surfactant. [ABSTRACT FROM AUTHOR]

Bile Salts (BS) adsorb onto emulsified oil droplets to promote lipolysis and then desorb, solubilizing lipolytic products, a process which plays a crucial role in lipid digestion. Hence, investigating the mechanism of adsorption and desorption of BS onto the oil–water interface is of major importance to understand and control BS functionality. This can have implications in the rational design of products with tailored digestibility. This study shows the adsorption and desorption curves of BS at air–water and oil–water interfaces obtained by pendant drop tensiometry. Three BS have been chosen with different conjugation and hydroxyl groups: Sodium Taurocholate (NaTC), Glycodeoxycholate (NaGDC) and Sodium Glycochenodeoxycholate (NaGCDC). Experimental results show important differences between the type of BS and the nature of the interface (air/oil–water). At the air–water interface, Glycine conjugates (NaGDC and NaGCDC) are more surface active than Taurine (NaTC), and they also display lower surface tension of saturated films. The position of hydroxyl groups in Glycine conjugates, possibly favors a more vertical orientation of BS at the surface and an improved lateral packing. These differences diminish at the oil–water interface owing to hydrophobic interactions of BS with the oil, preventing intermolecular associations. Desorption studies reveal the presence of irreversibly adsorbed layers at the oil–water interface in all cases, while at the air–water interface, the reversibility of adsorption depends strongly on the type of BS. Finally, dilatational rheology shows that the dilatational response of BS is again influenced by hydrophobic interactions of BS with the oil; thus, adsorbed films of different BS at the oil–water interface are very similar, while larger differences arise between BS adsorbed at the air–water interface. Results presented here highlight new features of the characteristics of adsorption layers of BS on the oil–water interface, which are more relevant to lipid digestion than characteristics of BS adsorbed at air–water interfaces. [ABSTRACT FROM AUTHOR]

The knowledge of interaction of a drug with the membrane phospholipids is crucial to decipher its penetration mechanism inside the cell. Tetracycline is a widely used antibiotic effective against various classes of bacteria. In this work, we have employed vibrational sum-frequency generation (VSFG) spectroscopy to understand the molecular-level interaction of tetracycline with a model phospholipid monolayer, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), at the air–water interface. Results suggest that tetracycline molecules are not present on the topmost surface of the water and remain mostly in the bulk aqueous phase. However, due to charge–dipole interaction, the orientation of water molecules in the subphase immediately beneath the topmost layer gets randomized due to its presence. Due to their long hydrophobic aliphatic chains, DPPC molecules are surface active and replace the topmost water layer and increase the polar orientation of interfacial water molecules by generating electric field across the interface. In the presence of the DPPC monolayer, tetracycline molecules get adsorbed from the bulk water to the interface due to electrostatic interactions with the ionic headgroups of DPPC. This interaction also results in the ordering of hydrophobic chains as confirmed by an increase in the VSFG intensity of CH3 groups of the hydrocarbon chains. We have been successful in unraveling the changes induced in the hydrocarbon backbone of DPPC as a result of such interaction. The water molecules associated with the DPPC monolayer also get randomized in presence of tetracycline molecules. We, for the first time, have studied the dynamics of interfacial water molecules in presence of tetracycline and tetracycline-DPPC environment. [ABSTRACT FROM AUTHOR]

• The quantum chemical calculations and ab initio dynamics simulation were adopted to clarify the gas phase and air-water interface reaction mechanisms of CIs and HCHO. • The HCHO could be a significant tropospheric scavenger of CIs and an inhibitor in the CIs oxidizing ability on SOx in dry and highly polluted regions. • The BHMP formation from CH2OO and HCHO at the air-water interface was confirmed and the corresponding reaction mechanism was proposed. • At the water's surface, the reaction channels are completed on picosecond time scales and follow a loop structure mechanism. Given the high abundance of water in the atmosphere, the reaction of Criegee intermediates (CIs) with (H 2 O) 2 is considered to be the predominant removal pathway for CIs. However, recent experimental findings reported that the reactions of CIs with organic acids and carbonyls are faster than expected. At the same time, the interface behavior between CIs and carbonyls has not been reported so far. Here, the gas-phase and air-water interface behavior between Criegee intermediates and HCHO were explored by adopting high-level quantum chemical calculations and Born-Oppenheimer molecular dynamics (BOMD) simulations. Quantum chemical calculations evidence that the gas-phase reactions of CIs + HCHO are submerged energy or low energy barriers processes. The rate ratios speculate that the HCHO could be not only a significant tropospheric scavenger of CIs, but also an inhibitor in the oxidizing ability of CIs on SO x in dry and highly polluted areas with abundant HCHO concentration. The reactions of CH 2 OO with HCHO at the droplet's surface follow a loop structure mechanism to produce i) SOZ (▪), ii) BHMP (HOCH 2 OOCH 2 OH), and iii) HMHP (HOCH 2 OOH). Considering the harsh reaction conditions between CIs and HCHO at the interface (i.e. , the two molecules must be sufficiently close to each other), the hydration of CIs is still their main atmospheric loss pathway. These results could help us get a better interpretation of the underlying CIs-aldehydes chemical processes in the global polluted urban atmospheres. Graphical Abstract [Display omitted] [ABSTRACT FROM AUTHOR]

Hydroxyl radical (OH) is a key oxidant that triggers atmospheric oxidation chemistry in both gas and aqueous phases. The current understanding of its aqueous sources is mainly based on known bulk (photo)chemical processes, uptake from gaseous OH, or related to interfacial O3 and NO3 radical-driven chemistry. Here, we present experimental evidence that OH radicals are spontaneously produced at the air-water interface of aqueous droplets in the dark and the absence of known precursors, possibly due to the strong electric field that forms at such interfaces. The measured OH production rates in atmospherically relevant droplets are comparable to or significantly higher than those from known aqueous bulk sources, especially in the dark. As aqueous droplets are ubiquitous in the troposphere, this interfacial source of OH radicals should significantly impact atmospheric multiphase oxidation chemistry, with substantial implications on air quality, climate, and health. [ABSTRACT FROM AUTHOR]

Although high-resolution single-particle cryo-electron microscopy (cryo-EM) is now producing a rapid stream of breakthroughs in structural biology, it nevertheless remains the case that the preparation of suitable frozen-hydrated samples on electron microscopy grids is often quite challenging. Purified samples that are intact and structurally homogeneous - while still in the test tube - may not necessarily survive the standard methods of making extremely thin, aqueous films on grids. As a result, it is often necessary to try a variety of experimental conditions before finally finding an approach that is optimal for the specimen at hand. Here, we summarize some of our collective experiences to date in optimizing sample preparation, in the hope that doing so will be useful to others, especially those new to the field. We also hope that an open discussion of these common challenges will encourage the development of more generally applicable methodology. Our collective experiences span a diverse range of biochemical samples and most of the commonly used variations in how grids are currently prepared. Unfortunately, none of the currently used optimization methods can be said, in advance, to be the one that ultimately will work when a project first begins. Nevertheless, there are some preferred first steps to explore when facing specific problems that can be more generally recommended, based on our experience and that of many others in the cryo-EM field.

Despite significant advances in all aspects of single particle cryo-electron microscopy (cryo-EM), specimen preparation still remains a challenge. During sample preparation, macromolecules interact with the air-water interface, which often leads to detrimental effects such as denaturation or adoption of preferred orientations, ultimately hindering structure determination. Randomly biotinylating the protein of interest (for example, at its primary amines) and then tethering it to a cryo-EM grid coated with two-dimensional crystals of streptavidin (acting as an affinity surface) can prevent the protein from interacting with the air-water interface. Recently, this approach was successfully used to solve a high-resolution structure of a test sample, a bacterial ribosome. However, whether this method can be used for samples where interaction with the air-water interface has been shown to be problematic remains to be determined. Here we report a 3.1 Å structure of an RNA polymerase II elongation complex stalled at a cyclobutane pyrimidine dimer lesion (Pol II EC(CPD)) solved using streptavidin grids. Our previous attempt to solve this structure using conventional sample preparation methods resulted in a poor quality cryo-EM map due to Pol II EC(CPD)'s adopting a strong preferred orientation. Imaging the same sample on streptavidin grids improved the angular distribution of its view, resulting in a high-resolution structure. This work shows that streptavidin affinity grids can be used to address known challenges posed by the interaction with the air-water interface.

[Display omitted] Phospholipids are the protective layer of modern cells, but it is challenging for the formation of phospholipids that require a simple abiotic synthesis before the advent of primitive cells. Here, we reported the abiotic synthesis for lysophosphatidic acids (LPAs) with prebiotically plausible reactants in aqueous microdroplets under ambient conditions. The LPAs formation is carried out by fusing two microdroplets streams: one contains glycerol and pyrophosphate in water and the other one contains fatty acids in acetonitrile. Compared with the bulk solution, LPAs were generated in microdroplets without the addition of catalyst and heating. Conditions of reactant concentrations and microdroplet size varied and suggested that LPAs formation occurred near or at the microdroplet surface. The LPAs formation also showed chemoselective toward on chain-length of fatty acids. Finally, the formation of LPAs underwent two-step reactions with glycerol phosphorylation eliminating one water molecule followed by esterification with fatty acids. These results also implicated that pyrophosphate functioned as both catalysts and precursors in prebiotic LPAs synthesis. The approach using fusion aqueous microdroplets has desirable features in studying the substance exchange and interaction in atmosphere. [ABSTRACT FROM AUTHOR]

A filling operation generates continuous changes over the shape of an air–water interface, which can be captured using a 3D CFD model. This research analyses the influence of different hydro-pneumatic tank pressures and air pocket sizes as initial conditions for studying rapid filling operations in a 7.6 m long PVC pipeline with an irregular profile, using the OpenFOAM software. The analysed scenarios were validated using experimental measurements, where the 3D CFD model was suitable for simulating them. In addition, a mesh sensitivity analysis was performed. Air pocket pressure patterns, water velocity oscillations, and the different shapes of the air–water interface were analysed. [ABSTRACT FROM AUTHOR]

Interfaces, the boundary that separates two or more chemical compositions and/or phases of matter, alters basic chemical and physical properties including the thermodynamics of selectivity, transition states, and pathways of chemical reactions, nucleation events and phase growth, and kinetic barriers and mechanisms for mass transport and heat transport. While progress has been made in advancing more interface‐sensitive experimental approaches, their interpretation requires new theoretical methods and models that in turn can further elaborate on the microscopic physics that make interfacial chemistry so unique compared to the bulk phase. In this review, we describe some of the most recent theoretical efforts in modeling interfaces, and what has been learned about the transport and chemical transformations that occur at the air–liquid and solid–liquid interfaces. This article is categorized under:Structure and Mechanism > Reaction Mechanisms and CatalysisStructure and Mechanism > Computational Materials ScienceSoftware > Quantum ChemistrySoftware > Simulation Methods [ABSTRACT FROM AUTHOR]

Although aqueous foam is composed of simple fluids, air and water, it shows a complex rheological behavior. It exhibits solid-like behavior at low shear and fluid-like behavior at high shear rate. Therefore, understanding such behavior is important for many industrial applications in foods, pharmaceuticals, and cosmetics. Additionally, air–water interface of bubble surface plays an important role in the stabilizing mechanism of foams. Therefore, the rheological properties associated with the aqueous foam highly depend on its interfacial properties. In this review, a systematic study of aqueous foam are presented primarily from rheology point of view. Firstly, foaming agents, surfactants and particles are described; then foam structure was explained, followed by change in structure under applied shear. Finally, foam rheology was linked to interfacial rheology for the interface containing particles whose surface properties were altered by surfactants. [ABSTRACT FROM AUTHOR]

[Display omitted] Although the dynamics of colloids in the vicinity of a solid interface has been widely characterized in the past, experimental studies of Brownian diffusion close to an air–water interface are rare and limited to particle-interface gap distances larger than the particle size. At the still unexplored lower distances, the dynamics is expected to be extremely sensitive to boundary conditions at the air–water interface. There, ad hoc experiments would provide a quantitative validation of predictions. Using a specially designed dual wave interferometric setup, the 3D dynamics of 9 μ m diameter particles at a few hundreds of nanometers from an air–water interface is here measured in thermal equilibrium. Intriguingly, while the measured dynamics parallel to the interface approaches expected predictions for slip boundary conditions, the Brownian motion normal to the interface is very close to the predictions for no-slip boundary conditions. These puzzling results are rationalized considering current models of incompressible interfacial flow and deepened developing an ad hoc model which considers the contribution of tiny concentrations of surface active particles at the interface. We argue that such condition governs the particle dynamics in a large spectrum of systems ranging from biofilm formation to flotation process. [ABSTRACT FROM AUTHOR]

The Sequential Quantum Mechanics/Molecular Mechanics scheme has been enacted to perform a systematic investigation of the polarizability (α) and first hyperpolarizability (β) responses at the water–vacuum interface. After performing classical molecular dynamics simulations to provide snapshots of the structures, quantum chemistry calculations of the linear and nonlinear optical responses have been performed for clusters of five water molecules at the time‐dependent DFT level in combination with different embedding schemes, ranging from point charges to polarizable point charges, with and without local field effects. When going from the bulk to the interface, the main observations of these calculations encompass i) a modest increase of the average polarizability but an increase by about a factor of two of its anisotropy, ii) an increase by about 20 % of the βHRS response, accompanied by a small increase of its depolarization ratio, and iii) a net increase of the component of the β tensor normal to the interface (βzzz) as well as of β//. Globally, the interfacial effects on β are localized at the first molecular layer while they are observed up to the fourth molecular layer on α. [ABSTRACT FROM AUTHOR]

This review will focus on micromachines and microrobots, which are objects at the micro-level with similar machine functions, as well as nano-level objects such as molecular machines and nanomachines. The paper will initially review recent examples of molecular machines and microrobots that are not limited to interfaces, noting the diversity of their functions. Next, examples of molecular machines and micromachines/micro-robots functioning at the air-water interface will be discussed. The behaviors of molecular machines are influenced significantly by the specific characteristics of the air-water interface. By placing molecular machines at the air-water interface, the scientific horizon and depth of molecular machine research will increase dramatically. On the other hand, for microrobotics, more practical and advanced systems have been reported, such as the development of microrobots and microswimmers for environmental remediations and biomedical applications. The research currently being conducted on the surface of water may provide significant basic knowledge for future practical uses of molecular machines and microrobots. [ABSTRACT FROM AUTHOR]

• PFAS sorption to the air-water interface (AWI) controlled loading to groundwater. • The impact of AWI sorption was greatest for long-chain PFAS at low effluent rates. • AWI sorption had a negligible impact for short-chain PFAS at high effluent rates. • The median PFAS enrichment factors in groundwater-effluent ranged from 1.3 to 5.2. • Groundwater PFAS concentrations exceeded the enforceable levels for drinking water. Fate and transport of per- and polyfluoroalkyl substances (PFAS) in wastewater treatment plant (WWTP) effluent discharged to rapid infiltration basins (RIBs) is investigated using data from 26 WWTPs in Michigan, USA. PFAS were found to accumulate in groundwater downgradient from RIBs with median groundwater-effluent enrichment factors for ten commonly detected, terminal-form perfluoroalkyl acids (PFAAs) ranging from 1.3 to 5.2. Maximum contaminant levels for drinking water were exceeded in groundwater at all WWTPs with available PFAS data. Numerical models of unsaturated fluid flow and PFAS transport honoring RIB site properties, such as median vertical separation distance to the water table and a realistic range of area-normalized effluent fluxes, show long-chain PFAS undergo significant delays from air-water interface (AWI) adsorption, requiring up to 15 times longer to reach maximum mass flux to the saturated zone under low-flux conditions, where AWI area is 2.5 times greater. Short-chain PFAS commonly detected in effluent are only minimally affected by AWI adsorption and show little to no attenuation under high-flux conditions. The nonlinear inverse relationship between water content and AWI area highlights the important role of AWI adsorption in modulating unsaturated transport of long-chain PFAS to underlying groundwater due to the broad range of flux rates applied to RIB systems. [Display omitted] [ABSTRACT FROM AUTHOR]

[Display omitted] • Air–water interface impact on AOPs studied via AIMD and DFT. • More chlorine substituents lead to CPs accumulation at interface. • 2-CPs, 2,4,6-TCPs favor hydration while PCPs prefer hydroxylation. • Interface catalyzes CPs, CPs−, PFRs dimerization for dioxin chloride. • The •OH non-oxidative consumption reduces AOPs, enhances POPs. Advanced oxidation processes (AOPs) based on hydroxyl radicals (OH)-dominant pathway are the most important technologies for the removal of bio-recalcitrant organic pollutants in industrial wastewater. Air–water interface formed by aeration is one of the factors that affect the removal efficiency of pollutants in AOPs. In this work, the mechanistic and kinetic insights into the roles of air–water interface on the hydroxylation of three chlorophenols (CPs) in UV/H 2 O 2 process have been investigated via theoretical calculation and experimental methods. Results show that CPs is more prone to accumulate at the air–water interface as the number of chlorine substituents increases. Consequently, the hydration reaction of CPs occurring at the interfacial influence the electron transfer associated with OH, leading to the non-oxidative consumption of OH. This interfacial reaction diminishes degradation efficiency, resulting in the formation of persistent organic pollutants. Herein, in UV/H 2 O 2 degradation of 2,4,6-TCPs, persistent free radical (PFRs), as well as 1,3,7,9-TCDD and 2,3,6-Trichloro-4-(2,4,6-trichlorophenoxy)phenol, were identified using EPR spectroscopy and LC-Q-TOF-MS mass spectrometry, respectively. Altogether, this work provides a comprehensive understanding of the roles of aeration on OH-initiated degradation behavior of chlorinated phenol pollutants. [ABSTRACT FROM AUTHOR]

The transport of per - and polyfluoroalkyl substances (PFASs) through unsaturated source-zone soils is a critical yet poorly understood aspect of their environmental behavior. To date, most experimental studies have only focused on the equilibrium or non-equilibrium partitioning of PFASs to the air-water interface, or solid-phase based equilibrium or non-equilibrium transport. Currently, there are discrepancies between air-water interfacial partitioning (K ia) results measured using a drainage-based column method (which supports a Langmuir isotherm) when compared to measurements from alternative experimental methods (which support a Freundlich isotherm). We hypothesize that this discrepancy is the result of non-Fickian transport conditions developing during column tests using the drainage method, which reduces the magnitude of the apparent K ia (K ia,app) when estimated using the retardation factor correlation from breakthrough curve experiments. To test the validity of this hypothesis, the drainage method was implemented using PFOS in a sand column and compared with prior data collected using a quasi-saturated column method. Results demonstrate that the apparent K ia was reduced by 3 to 123-fold, resulting in up to 123-fold faster breakthrough of PFOS than predicted with the assumption of equilibrium adsorption to the air-water interface. A novel mobile-immobile model (MIM) of PFAS fate and transport was developed, incorporating a term for anomalously adsorbed solute in the mobile zone to explain highly anomalous data. The modelling results using a modified HYDRUS-1D software show that anomalous air-water interfacial adsorption and/or flowpath channelization are plausible mechanisms for accelerated transport of PFOS and support the application of a Freundlich isotherm for PFOS. Overall, non-Fickian transport mechanisms demonstrate the potential to accelerate PFOS transport through the vadose zone by up to a factor of 123 under specific circumstances. This work demonstrates the assumption of equilibrium adsorption to air-water interfaces, even for homogeneous laboratory experiments, is not necessarily valid. • Non-Fickian transport mechanisms accelerate the movement of PFOS by up to 120 fold in unsaturated systems. • A combined effect of mass transfer to immobile zones and poorly understood anomalous transport drive the acceleration. • Mass transfer limitations are more pronounced at lower saturations, higher velocities, and lower concentrations of PFOS. • Discrepancies between drainage-based measurements of K ia and other methods are resolved by non-equilibrium considerations. • The use of an Extended Langmuir (Freundlich-Langmuir) isotherm to predict PFAS K ia is supported. [ABSTRACT FROM AUTHOR]

Pulses are important protein sources in the current protein transition, but much of the behavior of pulse proteins in food systems (e.g. foam) is still unknown. This study compared the similarities and differences of the proteins from three common pulses: lentil, faba bean, and chickpea, at the molecular, mesoscopic and functionality levels. For each source, the proteins were extracted with a simple one-pot alkaline extraction, resulting in protein extracts with high protein content (67–85%) rich in globulins (68–81%) and albumins (19–32%). All pulse protein fractions were predominantly in a native state with high solubilities (83–91%), high denaturation enthalpies (8.9–10.1 J/(g protein)) and low aggregation levels. Lentil protein showed higher absolute value of the ζ-potential (−18.5 ± 1.2 mV) than faba bean (−13.2 ± 0.8 mV) and chickpea protein (−12.4 ± 1.2 mV). The behavior of all pulse proteins at the air-water interface including adsorption kinetics, interfacial dilatational rheology and interfacial microstructure was investigated and linked to their foaming properties. The pulse protein with the highest vicilin and convicilin content (39.5% in lentil protein) showed the shortest adsorption lag time (300 ms), and demonstrated the highest foam overrun (290 ± 17%). All three pulse protein fractions formed interfaces with disordered solid-like behavior and consisted of heterogeneous network structures. These structures were mostly comprised of intact globulin proteins for lentil and faba bean proteins, while mixtures of intact globulins and unfolded proteins/albumins were observed for chickpea protein. The interfacial stiffness, interfacial density and ζ-potential of these pulse proteins showed a high positive correlation with their foam stabilization properties. Lentil protein has the highest values of those parameters and showed the longest foam half-life time of 115 (± 22) min, while faba bean and chickpea proteins showed comparably lower values of those parameters and shared similarly shorter foam half-life times of 46 (± 19) min and 38 (± 6) min, respectively. These findings can aid to provide clearer directions in screening pulse proteins for desirable performance in aerated food products. [Display omitted] • Lentil, faba and chickpea proteins were extracted in one-pot alkaline conditions. • These proteins have high solubility and nativity with varying globulin content. • Higher vicilin and convicilin content gives higher foamability due to faster adsorption. • Globulins of lentil and faba bean barely unfold at the air-water interface. • Surface stiffness, density and charge are positively correlated to foam stability. [ABSTRACT FROM AUTHOR]

High-intensity ultrasonication is an emerging technology for plant protein isolation and modification. In this study, the potential of temperature-controlled ultrasonication to enhance the recovery of functional proteins from potato trimmings was assessed. Different ultrasound energy levels [2000–40,000 J/g fresh weight (FW)] were applied during protein extraction at pH 9.0. True protein yields after ultrasonication significantly increased (up to 91%) compared to conventional extraction (33%). Microstructural analysis of the extraction residues showed more disrupted cells as ultrasonication time increased. Ultrasound treatments (10,000 and 20,000 J/g FW) increased the protein yield without affecting the foaming and air-water interfacial properties of protein isolates obtained after isoelectric precipitation (pH 4.0). However, proteins obtained after extended ultrasonication (40,000 J/g FW) had significantly slower early-stage adsorption kinetics. This was attributed to ultrasound-induced aggregation of the protease inhibitor fraction. In conclusion, ultrasonication shows potential to help overcome some challenges associated with plant protein extraction. [Display omitted] • Ultrasonication significantly increases the protein recovery from potato trimmings. • Temperature-controlled ultrasonication causes disruption of cellular structures. • The impact on the foaming properties of the isolated proteins is limited. • Potato protease inhibitors aggregate during extended ultrasonication. • Interfacial adsorption of isolated proteins is slower after extended ultrasonication. [ABSTRACT FROM AUTHOR]

The motion of magnetic particles under magnetic fields is an object to be solved in association with basic and practical phenomena. Movement phenomena of magnetite-encapsulated graphene particles at air–water interfaces were evaluated by manufacturing a feedback control system of the magnetic field to cause the motion of particles due to magnetic torque. A homogeneous magnetic field was generated using two pairs of electromagnets located perpendicular to each other, which were connected to an electronic switch. The system influenced the translational movement and the self-rotational speed of magnetic particles located at a center on the surface of fluid media in a continuous duty cycle. Operating the particle at a remote control in the same duty cycle at the air–water surface, the short and elongated magnetic particles successfully rotated. In addition, the rotational speed of the curved particle was slower than that of the elongated particle. The results indicate that the translational and self-rotational movements of magnetite-encapsulated graphene particles at the air–water interface under the external magnetic field are size- and shape-dependent for the speed and the direction. A short magnetic particle was used as a target particle to rotate on cancer cell lines, aiming to study the advantage of this method to induce the growth of HeLa cells. It was monitored for up to 4 days with and without magnetic particles by checking the viability and morphology of cells before and after the electromagnetic treatment. As an outcome, the movement of magnetic particles reduced the number of biological cells, at least on HeLa cells, but it was inactive on the viability of HeLa cells.