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ABSTRACT Anisakis simplex larvae, commonly found in marine fish, cause anisakiasis in humans, resulting in gastric to gastro‐allergic symptoms. Despite known health risks, the impact of Anisakidae larvae on fish hosts is less understood. This study aimed to investigate this interaction by assessing the feeding strategy of A. simplex. Anisakis larvae were isolated from North Sea Merluccius merluccius tissues (stomach, body cavity, liver and muscle) and were analysed for carbon (δ13C) and nitrogen (δ15N) isotope values. Significant differences in δ13C values were found among host tissues, with the liver differing from muscle and stomach tissues. In contrast, no differences were noted for the associated parasites. Additionally, δ15N values indicated that the host occupied a significantly higher relative trophic position than its parasite. This suggests a lack of direct nutrient transfer from host to parasite, as the parasite would typically exhibit higher stable isotope values than the tissue they feed on. Therefore, A. simplex's stable isotope values might reflect those of its previous host (crustacean and/or small fish), providing insights into diet and movement of the paratenic M. merluccius host. Further research is needed to confirm these findings across different fish species and to explore A. simplex as a proxy for trophic ecology. [ABSTRACT FROM AUTHOR]

Hysterothylacium aduncum is one of six pathogens responsible for human anisakiasis. Infection with H. aduncum can cause acute abdominal symptoms and allergic reactions and is prone to misdiagnosis in clinical practice. This study aims to enhance the efficiency and accuracy of detecting H. aduncum in food ingredients. We targeted the internal transcribed spacer 1 (ITS 1) regions of Anisakis to develop a visual screening method for detecting H. aduncum using recombinase polymerase amplification (RPA) combined with the CRISPR/Cas12a system. By comparing the ITS 1 region sequences of eight nematode species, we designed specific primers and CRISPR RNA (crRNA). The specificity of RPA primers was screened and evaluated, and the CRISPR system was optimized. We assessed its specificity and sensitivity and performed testing on commercial samples. The results indicated that the alternative primer ADU 1 was the most effective. The final optimized concentrations were 250 nM for Cas12a, 500 nM for crRNA, and 500 nM for ssDNA. The complete test procedure was achievable within 45 min at 37 °C, with a limit of detection (LOD) of 1.27 pg/μL. The amplified product could be directly observed using a fluorescence microscope or ultraviolet lamp. Detection results for 15 Anisakis samples were entirely consistent with those obtained via Sanger sequencing, demonstrating the higher efficacy of this method for detecting and identifying H. aduncum. This visual detection method, characterized by simple operation, visual results, high sensitivity, and specificity, meets the requirements for food safety testing and enhances monitoring efficiency. [ABSTRACT FROM AUTHOR]

In order to know whether there is a risk of anisakiasis (or anisakidosis) by consumption of fish of the genus Mullus from the western Mediterranean Sea, which are appreciated for their quality, an epidemiological survey was carried out to evaluate the occurrence of zoonotic or potentially zoonotic nematodes in M. barbatus and M. surmuletus. Although the presence of the third larval stage (L3) of anisakids (Anisakis and Contracaecum) has been previously described in these fish, the results showed the absence of anisakids and the presence, never in muscle, of L3 and L4 of raphidascaridids of the genus Hysterothylacium, molecularly identified as H. fabri. Phylogenetic analysis groups them into the Mediterranean Sea clade, far from individuals isolated in the Pacific Ocean. Prevalence was slightly higher, but not significant, in M. barbatus versus M. surmuletus (72.3% vs 60.0%), but mean intensity (MI) and mean abundance (MA) parameters were approximately twice as high in M. barbatus as in M. surmuletus (MI 5.8 vs 2.8, p =.001; MA 4.2 vs 1.7, p <.001). The presence of the parasite seems to have different effects on these two sympatric species. In M. barbatus it seems to affect their growth, as it appreciably reduces the value of allometry coefficient in infected fish (2.78 vs. 2.18). On the other hand, in M. surmuletus the infection significantly (p <.04) affects the Fulton's condition factor, an indicator of the health status of the fish. It can be concluded that the ingestion of these fish by the people poses negligible risk of anisakiasis, but the consumer should continue to be urged to follow the rules of prevention against this illness. [ABSTRACT FROM AUTHOR]

The increasing presence of Anisakis spp. in fish is having significant implications for public health due to a rise in cases of anisakiasis. Given this situation, there is a critical need to develop new strategies to fight this parasite. Satureja montana L., commonly known as savory, is a plant recognized in folk medicine for its therapeutic activity, such as being antispasmodic and digestive, among other properties. The aim of this study was to assess the nematicide activity against A. simplex larvae of the essential oil from two varieties of S. montana (subsp. montana (SMM) and variegata (SMV)). The essential oils were obtained via hydro-distillation of the flowering aerial parts. In vitro assays demonstrated the complete inactivation of anisakis larvae after 24 h when exposed to both essential oils, along with a significant reduction in their penetration capacity. Moreover, both essential oils showed an inhibitory effect on acetylcholinesterase (AChE). No differences between the subspecies were observed in any of the assays. Hence, the nematicidal activity of essential oils could be attributed to their capacity to inhibit AChE. These findings suggest the potential of S. montana essential oil for therapeutic and food industry applications. [ABSTRACT FROM AUTHOR]

In order to know whether there is a risk of anisakiasis (or anisakidosis) by consumption of fish of the genus Mullus from the western Mediterranean Sea, which are appreciated for their quality, an epidemiological survey was carried out to evaluate the occurrence of zoonotic or potentially zoonotic nematodes in M. barbatus and M. surmuletus. Although the presence of the third larval stage (L3) of anisakids (Anisakis and Contracaecum) has been previously described in these fish, the results showed the absence of anisakids and the presence, never in muscle, of L3 and L4 of raphidascaridids of the genus Hysterothylacium, molecularly identified as H. fabri. Phylogenetic analysis groups them into the Mediterranean Sea clade, far from individuals isolated in the Pacific Ocean. Prevalence was slightly higher, but not significant, in M. barbatus versus M. surmuletus (72.3% vs 60.0%), but mean intensity (MI) and mean abundance (MA) parameters were approximately twice as high in M. barbatus as in M. surmuletus (MI 5.8 vs 2.8, p =.001; MA 4.2 vs 1.7, p <.001). The presence of the parasite seems to have different effects on these two sympatric species. In M. barbatus it seems to affect their growth, as it appreciably reduces the value of allometry coefficient in infected fish (2.78 vs. 2.18). On the other hand, in M. surmuletus the infection significantly (p <.04) affects the Fulton's condition factor, an indicator of the health status of the fish. It can be concluded that the ingestion of these fish by the people poses negligible risk of anisakiasis, but the consumer should continue to be urged to follow the rules of prevention against this illness. [ABSTRACT FROM AUTHOR]

Background: Anisakis spp. are zoonotic nematodes causing mild to severe acute and chronic gastrointestinal infections. Chronic anisakiasis can lead to erosive mucosal ulcers, granulomas and inflammation, potential tumorigenic triggers. How Anisakis exerts its pathogenic potential through extracellular vesicles (EVs) and whether third-stage infective larvae may favor a tumorigenic microenvironment remain unclear. Methods: Here, we investigated the parasite's tumorigenic and immunomodulatory capabilities using comparative transcriptomics, qRT-PCR and protein analysis with multiplex ELISA on human intestinal organoids exposed to Anisakis EVs. Moreover, EVs were characterized in terms of shape, size and concentration using classic TEM, SEM and NTA analyses and advanced interferometric NTA. Results: Anisakis EVs showed classic shape features and a median average diameter of around 100 nm, according to NTA and iNTA. Moreover, a refractive index of 5–20% of non-water content suggested their effective biological cargo. After treatment of human intestinal organoids with Anisakis EVs, an overall parasitic strategy based on mitigation of the immune and inflammatory response was observed. Anisakis EVs impacted gene expression of main cytokines, cell cycle regulation and protein products. Seven key genes related to cell cycle regulation and apoptosis were differentially expressed in organoids exposed to EVs. In particular, the downregulation of EPHB2 and LEFTY1 and upregulation of NUPR1 genes known to be associated with colorectal cancer were observed, suggesting their involvement in tumorigenic microenvironment. A statistically significant reduction in specific mediators of inflammation and cell-cycle regulation from the polarized epithelium as IL-33R, CD40 and CEACAM1 from the apical chambers and IL-1B, GM-CSF, IL-15 and IL-23 from both chambers were observed. Conclusions: The results here obtained unravel intestinal epithelium response to Anisakis EVs, impacting host's anthelminthic strategies and revealing for the first time to our knowledge the host-parasite interactions in the niche environment of an emerging accidental zoonosis. Use of an innovative EV characterization approach may also be useful for study of other helminth EVs, since the knowledge in this field is very limited. [ABSTRACT FROM AUTHOR]

Elasmobranch populations are in steep decline mainly due to overfishing bycatch, but parasites may accelerate the collapse of vulnerable and/or highly parasitized species. We therefore studied metazoan parasites of Rajidae from the northeast Atlantic: vulnerable Leucoraja fullonica, near‐threatened Raja brachyura, Raja clavata, Raja microocellata and Raja undulata, and least‐concerned Raja montagui and Leucoraja naevus. Overall prevalence varied from 19% for R. montagui to 100% for L. fullonica. Parasite communities differed between skate species, and prevalence and abundance were higher for L. fullonica, R. microocellata, and R. undulata. We recorded 11 parasite taxa in the study: three nematodes, six cestodes, one monogenean, and one myxosporean. Whatever the skate species, the parasite component community comprised at least two nematode taxa among Phocanema spp., Proleptus sp. and Anisakis simplex. DNA‐sequencing revealed that Phocanema azarasi and Phocanema krabbei both occurred in R. microocellata and R. undulata. Phocanema spp. was first recorded in L. fullonica, L. naevus, R. microocellata, R. montagui, and R. undulata, as Proleptus sp. in L. fullonica, and A. simplex in L. fullonica and R. clavata, Rockacestus sp. and Nybelinia sp. in R. undulata, and gill‐myxosporeans on L. fullonica, L. naevus, R. microocellata, and R. undulata. The occurrence of 16 new host–parasite associations suggests potential environmental changes. Information provided by trophically transmitted helminths confirmed an opportunistic skate diet based on crustaceans and fish. We discuss results in terms of host fitness loss, bioindicator role of parasites, and anisakiasis risk. We recommend incorporating parasitology in research to improve elasmobranch conservation. [ABSTRACT FROM AUTHOR]

Dietary changes expose consumers to risks from Anisakis larvae in seafood, leading to parasitic diseases and allergies. Anisakis is recognized by EFSA as a significant hazard, with potential oncogenic implications. Diagnostic advancements, like the Basophil Activation Test (BAT), enhance sensitivity and accuracy in identifying Anisakis sensitization, complementing traditional IgE tests. We conducted a cross-sectional study on patients with allergic symptoms from April 2021 to April 2023 at two outpatient clinics in western Sicily. Our goal was to assess the prevalence of Anisakis-related allergies and to identify risk profiles using specific Anisakis IgE and the BAT, especially in regions with high raw fish consumption. The study evaluated specific Anisakis IgE as a screening tool for Anisakis sensitization, using questionnaires, blood samples, and immuno-allergology analyses. Anisakis-specific IgE values were compared with the BAT results, with statistical analyses including Fisher's exact test and logistic regression. The results showed an 18.5% seroprevalence of Anisakis IgE, while the BAT as a second-level test showed 4.63%, indicating the BAT's superior specificity and accuracy. The study highlighted the importance of the BAT in diagnosing Anisakis sensitization, especially in cases of cross-reactivity with Ascaris and tropomyosin. The findings confirm the BAT's exceptional specificity in identifying Anisakis sensitization and support using Anisakis-specific IgE for population-based risk profiling. The BAT can effectively serve as a confirmatory test. [ABSTRACT FROM AUTHOR]

Historical seroprevalence data for Anisakis in Spain vary greatly depending on the sampling region owing to different fish consumption habits. As a result of European Regulation (EC) No. 853/2004, the Royal Decree 1420/2006 on the prevention of parasitosis by Anisakis in fishery products supplied by establishments that serve food to final consumers or to communities came into force in Spain. In this study, a prevalence study of Anisakis in Madrid has been conducted to verify the prophylactic effects of the application of the law. Sera from 500 blood donors from the Fundación Jiménez Díaz University Hospital (Madrid/2021–2023) were collected, and the levels of anti-Anisakis IgG, IgA, and IgE were analyzed by ELISA, comparing them with those obtained with 110 donors from the Red Cross and the "Gómez Ulla" Central Defense Hospital (Madrid/2001–2002). The percentages of positivity in the 2021–2023 donor group were IgG (13.6%), IgA (13.6%), and IgE (2.2%), while in the 2001–2002 donors they were positive for IgG (15.45%), IgA (14.54%), and IgE (11.65%). A reduction of more than 80% was observed in the prevalence of anti-Anisakis IgE in the healthy population of Madrid, which confirmed the positive effect of RD1420/2006, which was later incorporated into RD1021/2022. [ABSTRACT FROM AUTHOR]

Background: Anisakiasis, a zoonotic disease caused by the nematode Anisakis, poses a significant concern for public health, particularly in regions with high consumption of raw or undercooked fish. Case Presentation: We present a case report of a 41-year-old woman who developed severe abdominal symptoms, ultimately diagnosed with intestinal obstruction due to Anisakis infestation, requiring surgery. Despite the absence of prominent eosinophilia or specific radiological findings, the diagnosis was confirmed through histological examination, highlighting the importance of considering anisakiasis in patients with a history of raw seafood consumption. Conclusion: The case underscores the diagnostic challenges associated with anisakiasis, emphasizing the need for increased awareness among healthcare professionals and the public regarding the risks of consuming raw or undercooked seafood. Effective management requires a multidisciplinary approach, including clinical assessment, imaging studies, and histological evaluation, to ensure timely diagnosis and appropriate treatment. [ABSTRACT FROM AUTHOR]

This study investigated the Anisakis larvae infection in the scads (Decapterus spp.) in the Indian Ocean off the southern coast of East Java. A fish sample of approximately 450 individuals, consisting of three scad species, i.e., the Indian scad (D. russelli), the shortfin scad (D. macrosoma), and the redtail scad (D. kurroides), were obtained from Prigi and Muncar fishing port, East Java. Fish samples were measured individually for length and weight and subsequently dissected and examined in detail to detect the presence of Anisakis larvae in the abdominal cavity, intestinal tract, gonad, liver, and muscle. The collected larvae were preserved in 100% ethanol for the further identification process. The study indicates that the Decapterus spp. were receptive to the Anisakis larvae infection, with varying prevalence and mean intensity across species and locations. The redtail scad (D. kurroides) exhibited the highest prevalence and mean intensity; all of the sample were infected with Anisakis larvae (P= 100%), with a mean intensity of 22.54 larvae/individual.The shortfin scad (D. macrosoma), on the other hand, showed the lowest prevalence (P= 30.61%) and a mean intensity of MI = 1.29 larvae/individual. Most Anisakis larvae (52.30–76.61%) were located within the body cavity, followed by the digestive tract (16.78– 18.40%), and only a small number of the larvae were detected in the liver, gonads, and muscles. The morphological identification indicated that the larva infecting the scad was Anisakis Type I, and it was confirmed as Anisakis typica throughout molecular identification. Anisakis typica parasitizes the redtail and shortfin scads in the Indian Ocean off the southern coast of East Java, indicating genetic variation, which is shown by the differences in nucleotide composition. The differences in Anisakis larvae infection and its genetic variation might be developed as biological indicators for various ecological investigations of the Decapterus spp. [ABSTRACT FROM AUTHOR]

Anisakis can cause Anisakiasis in humans if raw or undercooked fish is consumed. Symptoms of infection may include vomiting, acute abdominal symptoms, or allergies. In this study, we collected 187 commercially available marine fish from the Yellow Sea, East China Sea, and South China Sea. Among them, 79 were found positive containing 520 Anisakis worms. The average prevalence rate was found 42% in this investigation. Ninety-two worms from different sea areas were selected and analyzed for identification, revealing the presence of five different species, which are Anisakis pegreffii, Hysterothylacium aduncum, Hysterothylacium zhoushanense, Hysterothylacium amoyense, and Hysterothylacium sp. In the meta-analysis, three databases: PubMed, CNKI, and BaiduXueshu were searched for surveys on the prevalence of Anisakis in Chinese waters from January 2000 to December 2023. A total of 26 studies were included in this analysis of which 25 publications were retrieved from different databases and one being the present study. The pooled prevalence of Anisakis was 45% among commercially available marine fish. Variances in the prevalence of Anisakis were noted among the four seas, with the highest rates in the East China Sea and the Bohai Sea, reaching 53% [0.38; 0.68] and 49% [0.36; 0.62], respectively. The Prevalence of Anisakis infection was significantly higher in astern parts such as Liaoning, Shanghai, and Zhejiang. Analysis of the host fish subgroups revealed that the orders of Anguilliformes, Scombriformes, and Gadiformes had high rates of infection. These findings suggest a significant prevalence of Anisakis, posing an increasing risk of infection for individuals. This study provides impactful information for implementing preventative measures against Anisakis. [ABSTRACT FROM AUTHOR]

Background: Human fishing activities have significantly affect environmental concern for marine ecosystems, conservation of marine mammals, and human health. Coastal cetaceans are highly vulnerable to ingestion of fishing gear, bycatching, or entanglement, all of which can be fatal for these animals. In particular, certain coastal dolphins and porpoises are heavily impacted by fishing gear such as angling gear or stownet, as their food often overlap with the target fish species of human fisheries. Case presentation: This study presents a case of an Indo-Pacific finless porpoise (Neophocaena phocaenoides) beached on the coast of Jeju Island, Republic of Korea, with ingestion of fishing gear and severe Anisakis infection. Although this species inhabits waters ranging from the Persian Gulf to Taiwan, several stranded carcasses have been reported on Jeju Island in recent years. Post-mortem computed tomography revealed a bundle of four fishing hooks in the forestomach, along with nylon lines and steel lines with connectors, which were assumed to be angling gear for Jeju hairtail (Trichiurus lepturus). Further necroscopic investigation revealed that the forestomach contained a large number of Anisakis spp. (Nematoda: Anisakidae). Histological examination revealed a thickened forestomach wall with pinpoint and volcanic ulcerations, a thickened layer of stratified squamous epithelium, and infiltrated stroma in the squamous epithelium. conclusions: This study emphasizes the urgent need to address the impact of fishing activities on marine mammals, marine litter pollution, and the bycatch problem in Korean seawater. In addition, the occurrence of N. phocaenoides in seawater around Jeju Island should be raised in future geographical ecology or veterinary pathology studies and when its distribution is updated. [ABSTRACT FROM AUTHOR]

Background: Anisakis are globally distributed, marine parasitic nematodes that can cause human health problems, including symptoms such as vomiting, acute diarrhea, and allergic reactions. As parasitic nematodes that primarily affect the patient's digestive tract, intestinal helminths can interact directly with the host microbiota through physical contact, chemicals, or nutrient competition. It is widely accepted that the host microbiota plays a crucial role in the regulation of immunity. Materials and methods: Nematodes collected from the abdominal cavity of marine fish were identified by molecular biology and live worms were artificially infected in rats. Infection was determined by indirect ELISA based on rat serum and worm extraction. Feces were collected for 16S rDNA-based analysis of microbiota diversity. Results: Molecular biology identification based on ITS sequences identified the collected nematodes as A. pegreffii. The success of the artificial infection was determined by indirect ELISA based on serum and worm extraction from artificially infected rats. Microbiota diversity analysis showed that a total of 773 ASVs were generated, and PCoA showed that the infected group was differentiated from the control group. The control group contained five characterized genera (Prevotellaceae NK3B31 group, Turicibacter, Clostridium sensu stricto 1, Candidatus Stoquefichus, Lachnospira) and the infected group contained nine characterized genera (Rodentibacter, Christensenella, Dubosiella, Streptococcus, Anaeroplasma, Lactococcus, Papillibacter, Desulfovibrio, Roseburia). Based on the Wilcoxon test, four processes were found to be significant: bacterial secretion system, bacterial invasion of epithelial cells, bacterial chemotaxis, and ABC transporters. Conclusion: This study is the first to analyze the diversity of the intestinal microbiota of rats infected with A. pegreffii and to determine the damage and regulation of metabolism and immunity caused by the infection in the rat gut. The findings provide a basis for further research on host-helminth-microbe correlationships. [ABSTRACT FROM AUTHOR]

Fish health is of significant ecological and economic importance. In response to public observations of parasite-like structures in a popular edible fish, this study aimed to characterize nematode larvae commonly found in the muscle and body cavity of Saurida tumbil (Bloch, 1795), a commercially important fish species inhabiting the Persian Gulf and Oman Sea. This fish, locally known as Hasoom, holds substantial culinary importance, being a staple in the diets of millions residing in countries around the Persian Gulf. A total of 458 Saurida tumbil specimens were obtained from fish markets between June 2022 and May 2023. Subsequent examination revealed the presence of a total of 6132 nematode larvae. Nematodes found in the body cavity were identified as belonging to the genus Hysterothylacium sp., family Raphidascarididae, while those in the muscle were identified as Anisakis sp. larval type, family Anisakidae. Histopathology results suggested that these parasites may have adverse health impacts on their fish host. Notably, both nematode genera were found in the third larval stage, which is known to be the infective stage for anisakidosis. Given the reported cases of anisakidosis among people living in the study region, it is strongly recommended that fish be properly cooked before consumption to mitigate health risks. [ABSTRACT FROM AUTHOR]

Many pathogens are related to carcinogenesis. Chronic inflammation, as a result of persistent infection, leads to DNA damage, higher expression of oncogenes, decreased apoptosis and immunosuppression, which are some of the reasons for cancer induction. Among parasites, Schistosoma, Opistorchis and Clonorchis are recognised as infectious agents which contribute to cancer. A relationship between Anisakis and cancer was hypothesised because cellular responses to Anisakis products could result in inflammation and DNA damage. Previous research has shown a decrease in CD8+ γδ T-cells and an increase in αβ and γδ T-cell apoptosis in colon cancer (CC) samples. Ninety-two CC patients and 60 healthy subjects were recruited. γδ and αβ T-cells were analysed, and their apoptosis was evaluated. Anti-Anisakis antibodies were tested in sera from CC patients and controls. Anti-Anisakis IgG, IgM, IgA and IgE antibodies were significantly higher in CC patients. A significant increase in anti-Anisakis IgA levels was observed in patients with angiolymphatic invasion. The number of all γδ T-cells, as well as CD3+ CD4+ αβ T-cells, was significantly lower in CC patients. The apoptosis of all T-cells was significantly increased in patients with CC. We observed a significantly higher percentage of anti-Anisakis IgE positive patients having a deficit of CD3+ γδ T-cells. Our results suggest a relationship between Anisakis and CC. [ABSTRACT FROM AUTHOR]

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We here describe the location of anisakid third stage larvae in Atlantic herring Clupea harengus L. caught in the North Sea in August 2023. We further demonstrate how industrial processing (mechanical gutting, removal of entrails, head, tail, hypaxial anterior musculature and vertebral column) reduces the overall infection and worm load in the musculature. The isolated anisakid larvae were identified as Anisakis simplex sensu stricto by a combination of morphometrics and molecular methods (PCR of rDNA and mtDNA, sequencing, BLAST analysis). As a baseline we examined a total of 75 specimens of freshly caught and ungutted herring and showed a positive correlation between host size (fish length and weight) and infection level. The overall prevalence of infection was 84 %, the mean intensity 11.3 (range 1–38 parasites per fish) and the abundance 9.52. The main part of the overall worm population was associated with stomach and pyloric caeca in the body cavity (77 %) and only 5 % was found in the musculature. Larvae occurred in the hypaxial part of the musculature (21), the epaxial part (7 worms) and the caudal part (5 worms). The prevalence of muscle infection was 28 % and the mean intensity 1.6 (range 1–5) parasites per fish and abundance 0.44 parasites per fish. In order to assess the effect of industrial processing on worm occurrence in the fish we examined a total of 67 specimens of herring, from exactly the same batch, but following processing. This included removal of organs in the body cavity, cutting the lower part of the hypaxial segment but leaving the right and left musculature connected by dorsal connective tissue. Five out of these fish carried one larva (prevalence 7.5%, mean intensity 1, abundance 0.07 larvae per fish), and these worms were located in the ventral part of the anterior musculature (2), in the central part of the anterior musculature (2) and one larva in the central part of the caudal musculature. The industrial processing reduced the overall occurrence (abundance) of worms in the fish from 9.52 to 0.07 (136 times reduction) and the occurrence in the musculature from 0.44 to 0.07 (6.28 times reduction). The overall prevalence was reduced from 84 % to 7.5 % (11.2 times reduction). Muscle infection prevalence fell from 28 % to 7.5 % (3.7 times reduction). We then followed another batch of herring following a marinating process (11% NaCl for 24 h and subsequent incubation in acetic acid and vinegar) by artificially digesting the flaps during week 1–8. Although a total of 31 larvae were recovered from 144 fish examined no live nematode larvae were isolated. The importance of fish handling, processing and marination for consumer safety is discussed.

Strandings provide valuable information about rare marine organisms and their relationship with their environment. In the southern Gulf of México, specifically on the central coast of Campeche, Mexico, strandings of oceanic cetaceans have rarely been recorded, therefore, biological information has also been scarce. One of such rare stranding cases occurred on September 7, 2022, on the coast of Seybaplaya, Campeche. The specimen was identified as a female of Tursiops truncatus with the morphology features of the oceanic ecotype. The carcass was examined shortly after death, and samples for different analyses were obtained fresh. Tissue samples were collected, and some particular observations were made to define the individual's general health status. Results of the analyses revealed damage in the liver, lungs, and kidneys, probably related to 1) the advanced age of the organism (>35 years), and 2) the presence of persistent organic pollutants. Most importantly, this study contributed two new findings, the first record of the parasite Anisakis and the presence of renal calculi in the oceanic ecotype of T. truncatus in the southern Gulf of Mexico. [ABSTRACT FROM AUTHOR]

The longjaw tapertail anchovy Coilia nasus, which migrates from ocean to freshwater for spawning in spring, is an important anadromous fish with ecological and cultural significance. To determine parasite infection in anadromous C. nasus, a total of 103 fish from the Yangtze River were collected and examined in 2021 and 2022. The overall infection prevalence of nematodes in C. nasus was 100%, with a mean intensity of 13.81 ± 16.45. The mean intensity of nematode infections in 2022 was significantly higher than that observed in 2021 across all sampling sites (p < 0.05). Nematodes were widely detected in the mesentery, pyloric cecum, stomach, and liver, among which the mesentery accounted for the highest proportion, reaching up to 53.52%. A total of eight ascaridoid nematodes belonging to the family Anisakidae and Raphidascarididae were identified by using morphological characters and molecular biological techniques, including two species of Anisakis, five species of Hysterothylacium, and one species of Raphidascaris. A. pegreffii was found as the predominant species, accounting for 48.65% of all identified parasitic nematodes in liver, while Raphidascaris sp. was the most common nematode in the mesentery, pyloric cecum, and stomach, reaching up to 39.81%, 36.21%, and 74.36%, respectively. The present study systematically investigated the parasitic status and community structure of the nematode in C. nasus during its migration in the Yangtze River. This research provides a foundation for studying the impact of nematode parasitism on the reproductive migration and population recruitment of C. nasus, and offers valuable insights for biomarker screening and nematode identification in C. nasus. [ABSTRACT FROM AUTHOR]

This study reports on diseased Chelidonichthys spinosus fish that were originally obtained from the Taiwan Strait for exhibition in an aquarium. These fish showed abnormal swimming behavior, eye damage and skin lesions. Gross examination and histopathology revealed that the C. spinosus was suffering from severe nematode infections in the pyloric caeca, liver and mesentery. Marine nematodes from internal organs were verified by partially sequencing the internal transcribed spacer (ITS) gene. Sequencing results revealed that the nematodes shared 99.75% sequence identity with Raphidascaris lophii (GenBank accession No. MW132926). Additionally, secondary Vibrio harveyi infections were also detected in some C. spinosus. This is the first report of R. lophii infection in C. spinosus. [ABSTRACT FROM AUTHOR]

Background Anisakis infects humans by consuming contaminated undercooked or raw fish, leading to gastric anisakiasis, gastro-allergic anisakiasis, or asymptomatic contamination. Although larvae usually die when penetrating the gastric tissue, cases of intra- and extra-abdominal spread were described. We report the first probable case of pericardial anisakiasis. Case summary A 26-year-old man presented to the emergency department because of progressive lower limb oedema and exertional dyspnoea. Two months prior, he had consumed raw fish without any gastrointestinal symptoms. The echocardiogram reported a circumferential pericardial effusion ('swinging heart') and mildly reduced left ventricular ejection fraction (LVEF). He was diagnosed with myopericarditis after a cardiac magnetic resonance. A fluorodeoxyglucose positron emission tomography scan revealed an intense pericardial metabolism. Blood tests exhibited persistent eosinophilia and mild elevation of Anisakis simplex IgE—as for past infestation. A pericardial drainage was performed, subsequently, serial echocardiograms revealed a spontaneous recovery of his LVEF. No autoimmune, allergic, or onco-haematologic diseases were identified. Based on a history of feeding with potentially contaminated raw fish and on long-lasting eosinophilia, we suspected a pericardial anisakiasis, despite a low but persistent titre of specific IgE. Albendazole was administered for 21 days, along with colchicine and ibuprofen for 2 months; pericardial effusion resolution and eosinophil normalization occurred two weeks after. Discussion We hypothesized that Anisakis larvae may have migrated outside the gastrointestinal tract, penetrating the diaphragm and settling in the pericardium, causing pericarditis and pericardial effusion. Clinicians should know that the pericardium may be another extra-abdominal localization of anisakiasis, beyond pleuro-pulmonary involvement. [ABSTRACT FROM AUTHOR]

As gastrointestinal parasites affect the health of marine mammal species, they are a potentially consequential stressor. Although they are infrequently identified as a cause of mortality, gastrointestinal parasites could drive cryptic declines in individual fitness—including declines in nutritional status—by acting synergistically with other stressors. Nematodes in the family Anisakidae are ubiquitous throughout the world's oceans and are transmitted to marine mammal hosts through the consumption of fish and cephalopod prey. We sought to assess whether marine mammals face a rising risk of gastrointestinal infection due to an increase in anisakid burden of their prey species. We used data compiled from a recent meta‐analysis of anisakid abundance in fish and invertebrates from 1967 to 2015. We reviewed the diets of 113 marine mammal species to identify their known prey species. We extracted data on anisakid burden for each of the prey species and tested whether anisakid abundance in these intermediate hosts had increased over time. Our findings suggest that Anisakis spp. abundance has increased over the past half‐century, while Pseudoterranova spp. burden showed no significant change. Our findings suggest that the risk of Anisakis spp. infection is increasing for marine mammal hosts. [ABSTRACT FROM AUTHOR]

Consumption of raw and mildly processed seafood, in the context of modern Western world eating trends, is recognized as a major driver for human fish-borne infections. However, these zoonoses and their unfamiliar risks remain neglected and underappreciated among European diagnosticians. In contemporary Europe anisakidosis is one of the most important fish-borne zoonoses. It is caused by ingesting the third-stage infective larvae of the nematode parasites that belong to the family Anisakidae. The case described herein, is an intestinal and ectopic form of anisakiosis (Anisakis spp.), causing symptoms of subacute abdomen and masquerading as an intraperitoneal malignancy. It is the first anisakidosis case reported in Greece, affecting a young patient who had been repeatedly exposed to the parasite by consuming homemade raw fish. Right hemicolectomy, omentectomy and excision of a descending colon nodule were uneventfully performed. The pathology report confirmed granulomatous tissue with eosinophilic infiltration and parasites that were morphologically and molecularly identified as Anisakis spp. Although challenging, acquiring an accurate diagnosis of anisakidosis can prevent unnecessary surgery, as the infection typically is self-resolving, and if treatment is deemed necessary, it can be limited to antiparasitic medication. However, in rare cases, extra-gastrointestinal migration of larvae can cause severe damage with practically unknown risks, posing a diagnostic and therapeutic dilemma. In such a clinical case scenario, surgical exploration can decisively contribute to a definitive diagnosis and early identification of intraabdominal complications necessitating surgical intervention. [ABSTRACT FROM AUTHOR]

Introduction: Anisakiasis is a parasitic disease caused by larvae from anisakid nematodes. In recent years, there has been an increase in cases of anisakiasis, a relatively uncommon medical condition. Case Presentation: A 93-year-old woman with chronic heart failure developed hives and nausea 6 h after eating silver flounder sashimi, leading to suspicion of fish allergy. Despite treatment, symptoms persisted and progressed to abdominal pain. An upper gastrointestinal endoscopy revealed an Anisakis larva in her stomach, an unusual finding given her age. After endoscopic removal of the living worm, the patient's symptoms were relieved. Conclusion: A case of atypical anisakiasis in a 93-year-old patient was demonstrated. This case underscores the importance of considering atypical presentations in clinical decision-making. [ABSTRACT FROM AUTHOR]

Acute or chronic infections have been described among causes of chronic urticaria (CU). Anisakidosis is a human disease caused by the ingestion of larval nematodes of the family Anisakidae. The infestation is acquired by eating raw seafood or undercooked fish and squid. There are considerable variations in the frequency of underlying causes in the different studies and in different countries, such as differences in diets and the prevalence of infections. Anisakis simplex has been recognized as a trigger of both acute and CU manifestations. However, there is still a lack of evidence about its management and treatment in dermatology. We, therefore, reviewed some biologic properties of Anisakis simplex in order to understand the relationship between its biology and the mechanism it uses to establish chronic dermatological conditions such as urticaria and cause late complications. In addition, we herein report some concerns about the effectiveness of systemic treatment in preventing complications and management in dermatological settings. [ABSTRACT FROM AUTHOR]

The European anchovy Engraulis encrasicolus is one of the most important commercial species in the Bay of Biscay (ICES Subarea 8), and our analysis focused on the analysis of the temporal mean abundance, prevalence, and intensity of Anisakis spp. larvae species in anchovies from ICES Subarea 8 in the years 2000, 2001, 2014–2016, and 2019–2023. Prevalence in adult individuals of anchovy was only 1% in 2000 but increased to 90% in 2014. Since 2015, the prevalence has decreased, and the number of individuals affected in 2023 accounted for 17.6%. The mean abundance showed a similar trend, with a peak of 3.79 nematodes/anchovy in 2014, falling to 0.21 in 2023. The species A. simplex sensu stricto and A. pegreffii were identified by PCR/SANGER sequencing and PCR/RLFP techniques in 2019 and 2020. Anisakis simplex (s.s.) was the most abundant species and, according to the results returned by these two techniques, it accounted for an average of 62.4% and 52.1% of total nematodes in 2019 and 2020, respectively. The results of studies monitoring infection levels in anchovies showed that the mean abundance and prevalence changed over the course of the study period and that the proportion of different species of Anisakis is also subject to variation from year to year. [ABSTRACT FROM AUTHOR]

Food-borne zoonoses are of serious public health concern, with a high risk on human health in both developed and developing countries. Thus, this study aimed to determine the prevalence of zoonotic Anisakis larvae in smoked imported herring fish (Clupea harengus) and frozen mackerel (Scomber scomberus) from Assiut and Menoufia governorates, respectively in Egypt. All herring specimens were heavily infected with Anisakis larvae, while 9 out of 15 mackerel fish were infected. A total of 434 Anisakis larvae were collected from stomach, abdominal cavity, liver, between skin, muscles and gonads of 45 herring fish. In frozen mackerel, number of detected larvae was 78. Sequencing and phylogenetic analysis of internal transcribed spacer rDNA (ITS-rDNA) ribosomal DNA confirmed that the Anisakis larvae belonged to Anisakis simplex species. Furthermore, by employing light and scanning electron microscope, the morphological characters of Anisakis larvae were also determined. These findings highlight the importance of detecting health hazards in seafood products for helping in the management and prevention of anisakiasis. [ABSTRACT FROM AUTHOR]

A 53-year-old patient experienced 2 Anisakis-induced allergic episodes: the first with anaphylaxis, the second presenting with gastric symptoms and progressing to systemic anaphylaxis. The case could suggest a common pathophysiology involving allergic reactions in gastric anisakiasis and Anisakis allergy.

Globally, the exploitation of small pelagic fish, like Pacific mackerel is of great importance due to food industry demand. However, there are few studies regarding its parasites load and there are no in this geographic zone. This study aimed to assess the parasitic composition, some temporal changes (during spring and summer) in abundance, prevalence and intensity of infection parasitic of the Pacific mackerel (Scomber japonicus) from Todos Santos Bay, Baja California, Mexico. The parasite fauna of the Pacific mackerel consisted of 1930 parasites (1413 in spring and 517 in summer) distributed in the follow taxa: an Tetraphyllidea (Cestoda), Kuhnia scombri (Monogenea), Didymozoidae (Digenea), Anisakis sp. (Nematoda), Rhadinorhynchus sp. (Acanthocephala) and Caligus pelamydis (Copepoda). The nematodes parasite were the most abundant both in spring with a mean abundance of 27.6 parasites and in summer 8.2 parasites compared with the other taxa like Cestoda, Monogenea, Digenea, Acanthocephala and Copepoda (P = 0.003). The mean intensity of the nematodes in spring and summer was 28.1 and 13.4, respectively. The nematodes prevalence was 90 % in spring and 60 % in summer. In general, the parasite load is more abundant in spring than summer. In summer, absence of taxa as Cestoda and Copepoda were registered. Nematode larvae were present in the fish guts mesentery and inside of the stomach, pyloric caeca, intestine. Also the nematodes were found in the liver, muscle and gonads. The most affected organ by nematodes was the intestine mesentery. The most predominant parasite of this study has been Anisakis sp. during spring.

Anisakiasis is a parasitic disease caused by the ingestion of raw or uncooked seafood infected with third-stage larvae of anisakid nematodes. Generally, the larvae parasites live at the surface of the mucosa, but in this case, the larva deeply invaded its head into the gastric mucosa and was not removable with conventional biopsy forceps. In our case, we demonstrated the usefulness of jumbo forceps to remove the Anisakis larva in such a situation.

Historical seroprevalence data for Anisakis in Spain vary greatly depending on the sampling region owing to different fish consumption habits. As a result of European Regulation (EC) No. 853/2004, the Royal Decree 1420/2006 on the prevention of parasitosis by Anisakis in fishery products supplied by establishments that serve food to final consumers or to communities came into force in Spain. In this study, a prevalence study of Anisakis in Madrid has been conducted to verify the prophylactic effects of the application of the law. Sera from 500 blood donors from the Fundación Jiménez Díaz University Hospital (Madrid/2021–2023) were collected, and the levels of anti-Anisakis IgG, IgA, and IgE were analyzed by ELISA, comparing them with those obtained with 110 donors from the Red Cross and the “Gómez Ulla” Central Defense Hospital (Madrid/2001–2002). The percentages of positivity in the 2021–2023 donor group were IgG (13.6%), IgA (13.6%), and IgE (2.2%), while in the 2001–2002 donors they were positive for IgG (15.45%), IgA (14.54%), and IgE (11.65%). A reduction of more than 80% was observed in the prevalence of anti-Anisakis IgE in the healthy population of Madrid, which confirmed the positive effect of RD1420/2006, which was later incorporated into RD1021/2022.

Anisakid nematodes have been found in a variety of marine fishes worldwide and they are known to cause anisakiasis and allergic symptoms in human. One-hundred and five fish specimens of different sizes of red porgy (Pagrus pagrus) were collected randomly from Sharkia fish markets originating from the Gulf of Suez, Red Sea, Egypt and examined for the presence of ascaridoid nematodes. The overall prevalence of ascaridoids was 36.19% (38/105). Four morphotypes belonging to two genera of anisakid nematodes (Anisakis type I and Terranova type II larvae) as well as one genus of raphidascarid; Hysterothylacium (larvae and adult) were reported during this study. Hysterothylacium larvae were the most predominant species (23.81%) and abundance value =1.68. Interestingly, Pagrus pagrus was found as new host and locality records for larvae of Terranova type II. The results revealed a significant statistical correlation between fish length and number of the recovered ascaridoid nematodes (rs= 0.244, P < 0.05). There was a positive correlation between the host length and presence of Anisakis type I L3 (rs=0.276, P < 0.001). Significant differences were observed between the occurrence of Terranova type II L3 and Hysterothylacium L3 occurrence (rs= 0.388, P < 0.001) and adult (rs= 0.442, P < 0.001). In addition, a highly significant relationship was observed between the presence of Hysterothylacium L3 and adult (rs= 0.428, P < 0.001). Furthermore, the obtained results were lower than the recommended Egyptian standards (-100 parasites/fish), demonstrating that human health risk through consumption of fish flesh is not possible. [ABSTRACT FROM AUTHOR]

Fish play an important role in human nutrition. They are not only a great source of protein and healthy fats, but also a unique source of essential nutrients such as omega-3 fatty acids. Moreover, most fish are parasitized, and some of these parasites are able to influence the reallocation of resources in their favor and thus reduce the nutritional quality of the fish. The present study was conducted to investigate the impact of the third stage larvae (L3) of Anisakis spp. on the proximate composition, macro-minerals (potassium, calcium, and sodium), and fatty acids of European hake (Merluccius merluccius Linnaeus, 1758). In parasitized female group, our results revealed a decrease (p< 0.005) in the amount of carbohydrate by 6.5%, of calcium by 17%, and of 2 polyunsaturated fatty acids (arachidonic acid (C20: 4w-6), and eicosapentaenoic acid (C20: 5w-3) with 33% and 15% respectively. Simultaneously, an increase by 25% in the level of a single saturated fatty acid C10:0 was noticed. According to the principal component analysis, the parasitized female was wealthy of saturated fatty acids and monounsaturated fatty acids and contains less of polyunsaturated fatty acids, omega-3 fatty acids, and omega-6 fatty acids than the unparasitized female and male. No significant changes were observed in the biochemical composition of male hake, probably due to the low mean intensity of L3 larvae of Anisakis spp. in this group. [ABSTRACT FROM AUTHOR]

Anisakiasis is a parasitic disease caused by the ingestion of raw or uncooked seafood infected with third-stage larvae of anisakid nematodes. Generally, the larvae parasites live at the surface of the mucosa, but in this case, the larva deeply invaded its head into the gastric mucosa and was not removable with conventional biopsy forceps. In our case, we demonstrated the usefulness of jumbo forceps to remove the Anisakis larva in such a situation. [ABSTRACT FROM AUTHOR]

Anisakiosis is a significant zoonotic disease caused by parasitic nematodes of the Anisakis genus. It can be contracted by humans through the consumption of raw or undercooked fish contaminated with the parasite, leading to gastrointestinal and allergic symptoms. While anisakiosis is not frequently documented in Portugal, the presence of allergic reactions to Anisakis in Spain suggests ongoing exposure in the Iberian Peninsula. To address this concern, the Interdisciplinary Centre of Marine and Environmental Research in Porto, Portugal, in collaboration with the Biology Centre of Czech Academy of Sciences in Ceske Budejovice, Czech Republic, has proposed a project entitled 'Assessing Portuguese Health Risks: Anisakis Parasite in Atlantic Chub Mackerel (Scomber colias) Sold in Portuguese Markets' under the European Food Risk Assessment Fellowship Programme. The primary objective of the project is to gather valuable epidemiological data on the host, Atlantic chub mackerel (S. colias) and the parasitic nematode (Anisakis spp.) with the focus on assessing contamination levels and evaluating potential health risks associated with anisakiosis in the Portuguese population. By conducting this research, the project aims to contribute to the understanding of anisakiosis and its impact on public health in Portugal. Investigation of the presence of the Anisakis parasite in Atlantic chub mackerel sold in Portuguese markets will provide crucial insights into the risks associated with consuming raw or undercooked fish. Ultimately, our findings will aid in the development of preventive measures and guidelines to ensure the well‐being of the Portuguese population. [ABSTRACT FROM AUTHOR]

Red mullet (Mullus barbatus) is a commercially relevant fish species, yet epidemiological data on anisakid nematode infestation in M. barbatus are scarce. To fill this gap, we report the occurrence of Anisakis larvae in red mullet in the Ligurian Sea (western Mediterranean). This survey was performed between 2018 and 2020 on fresh specimens of M. barbatus (n = 838) from two commercial fishing areas (Imperia, n = 190; Savona, n = 648) in the Ligurian Sea. Larvae morphologically identified as Anisakis spp. (n = 544) were characterized using PCR-RFLP as Anisakis pegreffii. The overall prevalence of A. pegreffii was 24.46%; the prevalence at each sampling site was 6.32% for Imperia and 29.78% for Savona. Furthermore, 3300 larvae of Hysterothylacium spp. were detected in the visceral organs of fish coinfected with A. pegreffii, showing that coinfection with two parasitic species is not rare. This study provides a timely update on the prevalence of ascaridoid nematodes in red mullet of the Ligurian Sea, an important commercial fishing area in the Mediterranean. [ABSTRACT FROM AUTHOR]

The European conger, Conger conger, is a benthic marine fish species with a geographical distribution extending through the northeastern Atlantic and the Mediterranean. Despite being extensively distributed and widely appreciated by Spanish consumers, studies regarding parasite presence in this fish are scarce. In the present work, a hundred and eight specimens from the Mediterranean coast of northeastern Spain (Catalan waters) were surveyed for the presence of nematode parasites. Several species were morphologically identified: third-stage larvae of Anisakis type I (sensu Berland, 1961) (n = 131), third-stage larvae of Anisakis type II (sensu Berland, 1961) (n = 18), third- and fourth-stage larvae and adults of Hysterothylacium spp. (n = 48), adults of Cucullanus sp. (n = 391), and adults of Cristitectus congeri (n = 69). Moreover, some fish and decapode species were also observed as part of the host's diet, with the most detected preys being Micromesistius poutassou, Sardina pilchardus, Macropipus sp., and Goneplax rhomboides. This represents the first survey of nematode parasites infecting C. conger from the northeastern Spanish Mediterranean waters. Among the parasite species detected, the presence of Anisakis species should be highlighted as the ingestion of C. conger parasitized with these larvae could potentially lead to anisakiasis in consumers. [ABSTRACT FROM AUTHOR]

This research investigates the prevalence and mean intensity of Anisakis larvae on the bigeye scad, Selar crumenophthalmus (Bloch, 1793), along the Indian Ocean off Java. In total, 498 fish samples were collected from four locations: the Indian Ocean off East Java, Yogyakarta Special Territory, Central Java, and West Java. Each fish sample was measured for length and weight, then examined for Anisakis larvae in the abdominal cavity, liver, gonads, digestive tract, and muscle. Morphological characterisation was done using scanning electron microscope (SEM). The ITS rDNA region was amplified using PCR, then used to perform RFLP for identification and sequenced to form a phylogenetic tree. The results demonstrated that the bigeye scad was vulnerable to infection by Anisakis nematodes with varying prevalence and mean intensity at each location. The bigeye scad from the Indian Ocean off East Java had the highest prevalence and mean intensity (P = 75.44 %; MI = 10.38 larvae per-host), while from West Java had the lowest prevalence (P = 1.45 %; MI = 3.33 larvae per-host). The prevalence of Anisakis in the bigeye scad tends to decrease as one moves to the west from the sampling locations. Most larvae were found in the abdominal cavity (70 %-100 %), whereas a relatively low prevalence was found in other organs. The direct sequencing in the ITS rDNA region showed that Anisakis larvae found in the bigeye scad were A. typica (Diesing, 1860), which was indicated as A. typica var. indonesiensis (A. typica sp. B). Anisakis infection can be utilised as a biological indicator for movement patterns and fish stock differentiation. [ABSTRACT FROM AUTHOR]

Anisakiasis is caused by consuming raw fish contaminated with Anisakis sp. larvae and is extremely rare, especially when originating in the esophagus. We present a case of esophageal anisakiasis in a 61-year-old male who experienced severe precordial pain and radiating discomfort to the neck after consuming raw fish sashimi. Upper gastrointestinal endoscopy revealed the presence of a larva in the upper esophagus. On the basis of anatomo-morphological features, the worm was provisionally identified as Anisakis sp. and was easily extracted with forceps, which led to a prompt improvement in the patient's symptoms. This case highlights the importance of considering anisakiasis as a differential diagnosis in patients with gastrointestinal symptoms and a history of consuming raw fish. [ABSTRACT FROM AUTHOR]

Fish products in Slovakia have been heavily infected with Anisakis spp. larvae, which causes human anisakiasis. We found larvae in all tested samples of frozen Atlantic herring. Anisakid allergen t-Ani s7 testing revealed 2 positive cases in humans, signaling need for health authorities to closely monitor zoonotic marine parasites, even in inland areas.

In recent years, a drop in the condition of the European sardine has been observed. Although several causes have been attributed to this issue, as overfishing and climate change, little is known about the link between ascaridoid nematode parasitisation and fish status. In this study, sardines were obtained from four fishing grounds along the Mediterranean (Alboran, Northern Spain, Northern Adriatic, and Aegean), and one location in the Atlantic Ocean (Southern Portugal). After analysing individual fish body condition (by direct tissue fat content measurements and condition indices), and reproductive status (by a detailed gonadal examination) throughout the entire annual cycle, ascaridoids were recognised by combining naked eye and UV-press method along flesh, viscera, and gonads. Afterwards, sequence analysis of the rDNA internal transcribed spacers region (ITS) and the mtDNA cox2 gene were used to identify and characterise the different species of ascaridoids from the fish host in the localities throughout the seasons. The main species found along different areas was Hysterothylacium aduncum, present in the Northern Adriatic (prevalence of 7.6%, mean intensity 1.700), the Atlantic (7.5%, 3.889), and the Northern Spain (3.9%, 1.600). Moreover, few individuals of Anisakis simplex (s.s.) and A. pegreffii were observed in the Atlantic (1.7% and 0.8%, respectively), and the latter species was also found in the Adriatic stock (0.8%). All ascaridoid specimens were found in viscera. Obtained results seem to indicate that in stocks with medium sizes, small variations in length are related to parasite intensity. This study highlights the importance of seasonal parasitological analyses at stock level and, especially, in capital breeders, as relationships between condition and reproduction parameters and parasitism are conditioned by seasonality.

Abstract Anisakiosis is a significant zoonotic disease caused by parasitic nematodes of the Anisakis genus. It can be contracted by humans through the consumption of raw or undercooked fish contaminated with the parasite, leading to gastrointestinal and allergic symptoms. While anisakiosis is not frequently documented in Portugal, the presence of allergic reactions to Anisakis in Spain suggests ongoing exposure in the Iberian Peninsula. To address this concern, the Interdisciplinary Centre of Marine and Environmental Research in Porto, Portugal, in collaboration with the Biology Centre of Czech Academy of Sciences in Ceske Budejovice, Czech Republic, has proposed a project entitled ‘Assessing Portuguese Health Risks: Anisakis Parasite in Atlantic Chub Mackerel (Scomber colias) Sold in Portuguese Markets’ under the European Food Risk Assessment Fellowship Programme. The primary objective of the project is to gather valuable epidemiological data on the host, Atlantic chub mackerel (S. colias) and the parasitic nematode (Anisakis spp.) with the focus on assessing contamination levels and evaluating potential health risks associated with anisakiosis in the Portuguese population. By conducting this research, the project aims to contribute to the understanding of anisakiosis and its impact on public health in Portugal. Investigation of the presence of the Anisakis parasite in Atlantic chub mackerel sold in Portuguese markets will provide crucial insights into the risks associated with consuming raw or undercooked fish. Ultimately, our findings will aid in the development of preventive measures and guidelines to ensure the well‐being of the Portuguese population.

The purpose of this investigation was to identify the presence of parasite infections and cystic formation in Scomber japonicus, Scomber scombrus, and Thunnus thynnus. Euryphorus brachypterus and Clavellisa scombri were identified from Thunnus thynnus and Scomber scombrus, respectively. Anisakis pegreffii was identified from Scomber japonicus and Scomber scombrus by using molecular methods. Additionally, Anisakis typical was determined from Scomber japonicus. [ABSTRACT FROM AUTHOR]

Parasites play a key but overlooked role in the functioning of marine systems. Understanding the drivers of parasite infection in fish is thus crucial. Anisakis levels in the Atlantic horse mackerel Trachurus trachurus resulted from both biotic and environmental drivers. Ontogenic variation of the diet was the first driver of Anisakis level. The nonlinear pattern confirmed that the variation in parasitism was related to ontogenetic dietary shifts. The impact of depth on diet also explained the effect of depth on parasites. In deeper areas, fish only access pelagic prey, where Anisakis presence is high, consistent with the pelagic nature of the parasite. Finally, relative proportions of parasitic burden by tissue appeared to be driven by lipid content. In lipid-rich species like T. trachurus, Anisakis can find favorable conditions in the body cavity and in muscle and avoid the liver. Parasitism levels in fish are thus the result of patterns at play at different levels, and these factors should be better considered before being able to include parasites in an integrated vision of marine ecosystem functioning. [ABSTRACT FROM AUTHOR]

Anisakid nematode larvae are found in fish and cephalopods and can cause anisakiasis in humans when raw or undercooked seafood is consumed. An estimated 20,000 cases of anisakiasis occur annually in Japan. The only practical way to kill anisakis apart from heating has been freezing, but freezing can damage the texture and color of the fish meat. As an alternative to freezing, we have shown that anisakis in fish can be killed through the repeated application of pulsed high current through the fish meat, and that the quality of pulse-treated fish meat is almost the same as that of untreated meat. The following includes a description of a prototype device used to kill anisakid larvae using pulsed power technology. High temperatures can adversely affect fish meat quality. In order to apply the pulsed current while keeping the temperature of the fish meat as low as possible, we created a treatment tank filled with 180 liters of buffer saltwater, placed the fish meat between plane-to-plane electrodes in the treatment tank, and applied pulsed current while using pumps to agitate the water. Parameters such as saltwater conductivity, charging voltage, and capacitance necessary for killing anisakid larvae were studied using Taguchi methods, and the performance of the device was analyzed. To ensure worker safety, all parts of the device that may be touched by workers were grounded. The device was installed at a seafood processing factory where tests were conducted showing that all test larvae were killed. Currently, the company sells horse mackerel fillets treated with this device to some of its customers, and it is highly regarded for its safety and low impact on meat quality. [ABSTRACT FROM AUTHOR]

Parasites are concerning food-borne pathogens. Some of them are currently not being routinely controlled in food, probably because their burden on public health is underestimated and the relative importance of different transmission routes is not completely known. Parasitic incidences could be avoided if preventive technologies were applied during food processing. Effective inactivation treatments are currently based on heat or freezing, but their side effects collide head-on with current consumer trends and new culinary habits. This review describes the potential application of Pulsed Electric Field (PEF) technology in the control of food-borne parasites, with the aim of reducing the viability and infectivity of parasite transmission stages without affecting food quality. Results of published studies performed on different media are critically analyzed and factors affecting the outcomes are examined. Recent studies on the topic demonstrate the feasibility of PEF as an alternative to traditional freezing processes for the inactivation of Anisakis in fish. The development of new PEF equipment is advancing at a rapid pace, allowing for food treatment at a scale that would have been unimaginable some years ago. A review of more basic-science studies carried out on buffer media would contribute to progress in addressing the underlying drawbacks that remain to be solved. Thoroughly different fields (parasitology, physics, food engineering, water sanitation, etc.) should converge to achieve the industrial implementation of PEF for the inactivation of food-borne parasites. • The possibilities of parasite electroporation were critically analyzed. • Pulsed Electric Fields as an alternative to freezing for Anisakis killing in fish. • Studies on water sanitation and medicine were reviewed to help with food. • Equipment for food treatment implementation at the conditions required is available. [ABSTRACT FROM AUTHOR]

The genus Anisakis is among the most significant parasites to public health, as it causes anisakiasis, a parasitic infection in humans resulting from consuming raw or undercooked seafood. Although the infection status of Anisakis in second intermediate hosts, such as marine fishes and cephalopods, and humans have been severally reported in Korea, no information about the definitive host in Korean waters is available. In 2014, 2 adult gastric nematodes were collected from a common minke whale (Balaenoptera acutorostrata) found in the East Sea, Korea. These worms were identified as A. simplex sensu stricto (s.s.) by comparing the mitochondrial COX2 marker with previously deposited sequences. Phylogenetic and phylogeographic analyses of A. simplex (s.s.) worldwide revealed 2 distinct populations: the Pacific population and the European waters population. This is the first report on adult Anisakis and its definitive host species in Korea. Further studies on Anisakis infection in other cetacean species and marine mammals in Korean seas are warranted. [ABSTRACT FROM AUTHOR]