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6 results on '"biosurfaktanti"'

1. MODELING AND SIMULATION OF THE BIOSURFACTANT PRODUCTION BY ENZYMATIC ROUTE USING XYLOSE AND OLEIC ACID AS REAGENTS.

Author

MOULIN CANSIAN, ANA BÁRBARA, WALDIR TARDIOLI, PAULO, FERNANDO FURLAN, FELIPE, and DE SOUSA JÚNIOR, RUY

Subjects
*XYLOSE, *METHYL ethyl ketone, *FREE fatty acids, *BIOSURFACTANTS, *OLEIC acid, *INDUSTRIAL costs, *LIGNOCELLULOSE, *INDUSTRIAL wastes
Abstract

The biosynthesis of sugar esters, molecules with biosurfactant properties, can occur through the esterification of sugars with fatty acids by enzymatic catalysis. An alternative to reduce the impact of raw materials on the final biosurfactant production cost and the reuse of industrial waste is to use residues from vegetable oil industries as a source of free fatty acids, such as oleic acid, and lignocellulosic residues of 2G ethanol as a source of sugar (xylose). In this scenario, the present work aimed at modeling the biosurfactants production via heterogeneous biocatalysis using lipase, oleic acid, and xylose. Product separation and purification were performed using a sequence of precipitations (adding ethanol, water, and methyl ethyl ketone). The simulation was performed using the equation-oriented software EMSO (Environment for Modeling, Simulation, and Optimization), CAPE-OPEN compliant. The percentage of biosurfactants in the product was around 86%, with a recovery of 88% in the purification. Regarding the study of energy expenditure, a value of -604.1 kW of heat associated with cooling and a value of 137.6 kW associated with heating was observed. Developed mathematical models successfully described the process. The initial economic analysis of the process indicates a minimum biosurfactant selling price of US$ 72.37/kg. [ABSTRACT FROM AUTHOR]

Published
2022
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2. Screening and Characterization of Emulsifying HydrocarbonDegrading Bacteria from Coastal Waters of the Caspian Sea.

Author

Goncharova, Alla, Karpenyuk, Tatyana, Kalbayeva, Aliya, Mukasheva, Togzhan, and Bektileuova, Nurgul

Subjects
*TERRITORIAL waters, *SEAWATER, *COASTS, *BACTERIA, *ACHROMOBACTER, *BIOSURFACTANTS
Abstract

As a result of 400 cultures screening isolated from the contaminated coastal zones of the Caspian Sea, 4 new strains were selected that had a stable growth and utilized oil with NaCl concentration close to that of seawater. Stenotrophomonas chelatiphaga wkal49, Stenotrophomonas chelatiphaga wkal51, Sphingobacterium kitahiroshimense wkar54, and Achromobacter sp. wkar55 were identifi ed based on an analysis of the direct nucleotide sequence of the 16S rRNA gene fragment. The degree of oil degradation by these strains was above 50%. The hydrophobicity of the cell surface, emulsifying activity, the degree of infl uence on the viscosity of crude oil, and also the ability to produce surfactants were the four key factors that made up the studied parameters for the selected strains. The studied strains formed an emulsion layer on the surface of the culture medium. The most active producers of extracellular bioemulsifi ers were the Stenotrophomonas chelatiphaga wkal49 and Stenotrophomonas chelatiphaga wkal51 strains. Both demonstrated the highest hydrophobicity, emulsifi cation index, and the highest value for decreasing oil viscosity. [ABSTRACT FROM AUTHOR]

Published
2021
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3. Analiza potenciala sevov Bacillus spp. za biozaščito in pospeševanje rasti rastlin

Author

Benko, Bernarda and Mandić-Mulec, Ines

Subjects
biocontrol traits, Arabidopsis thaliana, phosphate solubilization, rizobakterije, siderophores, litični encimi, indol-3-ocetna kislina, biosurfaktanti, Bacillus, biofilm, udc:579.22/.26:579.852.1:631.461, raztapljanje fosfatov, biosurfactants, vpliv na rast rastlin, PGPR, siderofori, lytic enzymes, rhizobacteria, indole-3-acetic acid, biokontrolne lastnosti, effect on plant growth
Published
2020

4. Genotipska in fenotipska karakterizacija nabrežnih izolatov Bacillus subtilis

Author

Planinc, Jerneja and Mandić-Mulec, Ines

Subjects
ecotypes, biosurfaktanti, ferotipi, Dienesova linija, swarming, rojenje, medsebojno prepoznavanje, Dienes line, biosurfactants, udc:579.22/.26:579.852.11:577.2.083, pherotypes, hemolitična aktivnost, recognition patterns, hemolytic activity, BOX-PCR, ekotipi, Bacillus subtilis
Published
2020

6. Adhezivna svojstva dvaju sojeva Lactobacillus i fizikalno-kemijska svojstva njihovih biosurfaktanata

Author

Bartosz Brzozowski, Włodzimierz Bednarski, and Piotr Gołek

Subjects
Lactobacillus, adhezija, biosurfaktanti, Enterobacteriaceae, adhesion, biosurfactants, food and beverages
Abstract

The aim of this study is to describe the adhesive capability of Lactobacillus fermenti 126 and Lactobacillus rhamnosus CCM 1825 as well as to isolate and evaluate the functional properties of their synthesized biosurfactants. Fourier transform infrared spectroscopy shows that both crude biosurfactants contain three components: protein, polysaccharide and phosphate in different ratio. The crude biosurfactants synthesized by Lactobacillus fermenti 126 and Lactobacillus rhamnosus CCM 1825 contain 8 and 9 fractions analyzed by capillary gel electrophoresis. Lactobacillus fermenti 126 and Lactobacillus rhamnosus CCM 1825 strains used in this study synthesize biosurfactants with low effectiveness, critical micelle concentration of 9.0 and 6.0 g/L, and surface tension of (45.1±0.1) and (43.6±0.6) mN/m, respectively. Biosurfactant synthesized by Lactobacillus rhamnosus CCM 1825 demonstrated higher emulsifying and froth-forming activity than that obtained from Lactobacillus fermenti 126, which resulted in better antiadhesive properties. The advantageous adhesive properties of these Lactobacillus strains were confirmed. A positive effect of the impregnation of polystyrene surface with an aqueous solution of biosurfactants on the inhibition of adhesion of Escherichia coli 22, Klebsiella pneumoniae 2 and Pseudomonas aeruginosa W2 to the impregnated surface was found., Svrha je rada bila opisati adhezivna svojstva bakterija Lactobacillus fermenti 126 i Lactobacillus rhamnosus CCM 1825 i iz njih izolirati biosurfaktante, te procijeniti njihova funkcionalna svojstva. Fourierovom transformiranom infracrvenom spektroskopijom utvrđeno je da se oba biosurfaktanta sastoje od tri komponente: protein, polisaharid i fosfat u različitim omjerima. Neobrađeni biosurfaktanti iz Lactobacillus fermenti 126 i Lactobacillus rhamnosus CCM 1825 imaju 8 i 9 frakcija, koje su analizirane kapilarnom gel-elektroforezom. Biosurfaktant iz Lactobacillus fermenti 126 ima malu učinkovitost, kritičnu micelarnu koncentraciju od 9,0 g/L i površinsku napetost od (45,1±0,1) mN/m. Biosurfaktant iz Lactobacillus rhamnosus CCM 1825 također ima malu učinkovitost, kritičnu micelarnu koncentraciju od 6,0 g/L i površinsku napetost od (43,6±0,6) mN/m. Ima bolja emulgirajuća svojstva i svojstva pjenjenja, stoga bolje inhibira adheziju patogenih bakterija. Oba su soja dobro prianjala na podlogu, a impregnacijom površine od polistirena vodenom otopinom biosurfaktanata spriječena je adhezija patogenih bakterija Escherichia coli 22, Klebsiella pneumoniae 2 i Pseudomonas aeruginosa W2 na podlogu.

Published
2011

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