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Florida sugarcane production is mostly on organic soils, but the cultivation area on mineral soils is also expanding. Silicon (Si) is beneficial for sugarcane growth and development on weathered soil. However, the low solubility and availability of soluble Si is an issue in Florida's mineral soils. This study aimed to assess soil Si extraction methods based on sugarcane yield response to calcium silicate application. It also examined the relationship between sugarcane yield with extracted plant-available soil Si, alongside plant Si concentrations. The experiment followed a randomized complete block design involving 2 fields, with 24 plots each, and 6 replications. Four rates of calcium silicate (0 control, 2.24, 4.48, and 6.67 Mg ha−1) were applied before planting sugarcane. Soil samples were collected prior to planting and after each harvest. Soil Si was extracted using 0.5 M acetic acid, 0.7 N NH4OAc, Mehlich 3, and 0.01 M CaCl2 extractants. Leaf samples were collected over a three-year sugarcane life cycle (from 2019 to 2022) to evaluate the relationship between soil Si and leaf Si concentrations. The relationships between parameters were analyzed using linear and nonlinear regression models. Results indicated that higher Si application rates increased Si concentrations in sugarcane leaves in both fields. However, only one field showed a yield response to Si, likely due to high pre-plant soil Si in the other field. The best extractant should correlate strongly with either yield or leaf Si content, but no single extractant showed a strong correlation with either, making it infeasible to identify the best extractant. [ABSTRACT FROM AUTHOR]

Diabetic wounds are characterized by chronic inflammation, reduced angiogenesis, and insufficient collagen deposition, leading to impaired healing. Extracellular vesicles (EVs) derived from adipose-derived mesenchymal stem cells (ADSC) offer a promising cell-free therapeutic strategy, yet their efficacy and immunomodulation can be enhanced through bioactivation. In this study, we developed calcium silicate (CS)-stimulated ADSC-derived EVs (CSEV) incorporated into collagen hydrogels to create a sustained-release system for promoting diabetic wound healing. CSEV exhibited enhanced protein content, surface marker expression, and bioactive cargo enriched with pro-angiogenic and anti-inflammatory factors. In vitro, CSEV-loaded collagen significantly reduced reactive oxygen species production, promoted cell proliferation and migration compared to standard EV-loaded collagen. Cytokine profiling revealed the upregulation of anti-inflammatory cytokines and extracellular matrix components, highlighting their immunomodulatory and regenerative potential. In vivo, histological evaluation of diabetic rabbit models treated with CSEV-loaded collagen revealed superior reepithelialization and organized collagen deposition, indicating accelerated wound closure. These findings underscore the potential of CSEV-loaded collagen hydrogels as an innovative and effective therapeutic platform for enhancing diabetic wound healing by simultaneously addressing inflammation and tissue regeneration. [ABSTRACT FROM AUTHOR]

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement to enhance bone regeneration and modulate the immune microenvironment to promote tissue repair. We synthesized a LiCS ceramic powder and performed comprehensive analyses of its physicochemical properties, including phase composition, morphology, setting time, and mechanical strength. The results demonstrated that the incorporation of lithium into calcium silicate significantly increased the diametral tensile strength (DTS) and facilitated hydroxyapatite formation compared with undoped calcium silicate. In vitro assays revealed that the LiCS cement enhanced the proliferation, adhesion, and spread of Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS cement exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines and increasing anti-inflammatory cytokines, promoting the polarization of macrophages towards the M2 phenotype. The presence of Li in the cement also significantly improved the osteogenic differentiation of WJMSCs, as evidenced by elevated levels of alkaline phosphatase and osteocalcin expression. These findings underscore the dual functional capabilities of the LiCS cement in enhancing osteogenesis and modulating the immune environment, making it a promising material for bone tissue engineering and regeneration. [ABSTRACT FROM AUTHOR]

Environmental protection issues have received widespread attention, making material recycling increasingly important. The upcycling of polysulfone (PSF) medical waste, recognized as a high-performance plastic with excellent mechanical properties, deserves promotion. While PSF is suitable for use as an orthopedic implant material, such as internal fixation, its osteogenesis capabilities must be enhanced. Mechanical stability, particularly over the long term, is a significant concern for bone implants in load-bearing applications. This study recycled PSF medical waste to create bone composites by incorporating osteogenic calcium silicate (CaSi) at three different contents: 10%, 20%, and 30%. We evaluated the phase, morphology, weight loss, and three-point bending strength of the PSF-based composites after they were soaked in dynamic simulated body fluid (SBF) at pH levels of 7.4 and 5.0 for up to 12 months. Human mesenchymal stem cells (hMSCs) were utilized to assess the osteogenic activity of these composites. Our findings revealed that, while the bending strength of PSF-based composites declined with prolonged exposure to SBF, the dissolution of CaSi particles led to a manageable weight loss of about 4% after 12 months, regardless of pH 7.4 or 5.0. Importantly, the incorporation of CaSi into the PSF matrix exhibited a positive effect on the attachment and proliferation of hMSCs. The levels of alkaline phosphatase (ALP) and calcium deposits directly correlated with the CaSi content, indicating superior osteogenic activity. Considering biostability and osteogenic ability, the 20% CaSi-PSF composite demonstrated promise as a candidate for load-bearing implant applications. [ABSTRACT FROM AUTHOR]

Calcium silicate carbonatable binder is an important direction to realize the green and sustainable development of building materials industry. In order to study the effect of calcium silicon ratio (n(CaO):n(SiO2), molar ratio) on the composition of calcium silicate mineral phase and their carbon sequestration capabilities, calcium silicate carbonatable binders were synthesized with controlled calcium silicon ratio (n(CaO):n(SiO2)=1.8-2.2). Techniques such as t-pH, XRD, TGA, and SEM were employed to examine the evolution of phase composition, carbonization hardening process, CO2 uptakes and carbonization products of calcium silicate carbonatable binders. The results indicate that the content of γ-dicalcium silicate (Ca2SiO4, γ-C2S) initially increases and subsequently decreases as the calcium silicon ratio rises. When the calcium silicon ratio is no higher than 2.0, the content of rankinite (Ca3Si2O7, C3S2) gradually increases with the decreasing of calcium silicon ratio. When the calcium silicon ratio is higher than 2.0, the content of β-dicalcium silicate (Ca2SiO4, β-C2S) progressively increases with the increasing of calcium silicon ratio. The reaction of carbonization for 0.5 h is intense, and the compressive strength initial increases and subsequent decreases as the calcium silicon ratio increases. The reaction produces calcite, aragonite and vaterite. With the content of C3S2 and γ-C2S decreasing significantly, and the calcite content remains largely unchanged even when the carbonization reaction is extended to 24 h. C3S2 decelerates the early reaction of binder, but a more sustained carbonization reaction and a greater variety of carbonization products can enhance its subsequent compressive strength. The compressive strength and CO2 uptakes after carbonization for 24 h reach 129.76 MPa and 18.57%, respectively. [ABSTRACT FROM AUTHOR]

Objective: To assess physico-chemical properties of BioRoot RCS (powder-to-liquid formulation) and BioRoot Flow (pre-mixed formulation) after exposure to citric acid (CA) and EDTA. Methods: BioRoot RCS and BioRoot Flow specimens (5 × 2 mm) were incubated for 28 days at 37 °C before being treated with test solution (distilled water, 17% EDTA, 10% CA, 20% CA or 40% CA). Changes in mass, ion release (inductively coupled plasma optical emission spectroscopy), phase composition (X-ray diffraction and Raman spectroscopy), surface morphology (scanning electron microscopy and energy dispersive spectroscopy) were evaluated. The Shapiro–Wilk, Levene and one-way ANOVA, followed by Tukey’s test, were used for statistical analysis (p < 0.05). Results: The greatest mass loss, ion release and surface morphological changes (pores and cracks) of BioRoot RCS and BioRoot Flow occurred in 10% and 20% CA. No significant differences were identified between BioRoot RCS and BioRoot Flow in distilled water and EDTA (p > 0.05), whereas BioRoot Flow was significantly more soluble than BioRoot RCS in all CA solutions (p < 0.001). The main phase identified for both materials was zirconium oxide, which remained unaffected by the solutions. Conclusions: Within the limitations of the study, 10% and 20% CA were more effective than distilled water, 17% EDTA and 40% CA in terms of dissolving BioRoot RCS and BioRoot Flow. The BioRoot Flow was significantly more soluble in all CA solutions than BioRoot RCS. Clinical significance: 10% and 20% CA solutions may have a potential to be used as a solvent for hydraulic calcium silicate sealers during endodontic retreatment. [ABSTRACT FROM AUTHOR]

This study evaluated the effectiveness of three different irrigant activation techniques in cleaning and establishing patency during retreatment of root canals obturated with gutta‐percha and bioceramic sealer. 60 extracted premolars with oval‐shaped canals were instrumented and obturated with gutta‐percha and EndosequenceBC sealer using the 'warm hydraulic condensation' technique. The teeth were retreated using Protaper Universal Retreatment and XP‐Endo Shaper system and divided into four groups according to the irrigant activation protocol used: control, passive ultrasonic irrigation (PUI), Endovac irrigation (EVI) and XP‐Endo Finisher R (XPFR). Apical patency was achieved in all the samples of the XPFR group (100%), which showed a significantly higher success rate compared with the control (73.3%) and EVI groups (73.3%) (p < 0.05). The scanning electron microscopic evaluation revealed significantly cleaner middle and apical third root canals in the PUI and XPFR groups compared with the control group (p < 0.05). These findings suggest that XPFR effectively cleans and establishes patency in root canals filled with bioceramic sealers. [ABSTRACT FROM AUTHOR]

Marble and granite industries have expanded significantly over the past few decades, leading to an increase in processing waste. To reduce the environmental impacts of granite and marble processing waste, proper management and potential recycling with sustainable methodologies are crucial. The aim of this study is to synthesize nano-calcium silicates by utilizing a fine fraction of waste derived from the granite (rich in SiO2) and the marble (rich in CaO) processing industries, using a chemical-free solid-state reaction method. The research investigates the effects of three different mix proportions of CaO:SiO2 (1:0.9, 1:1, 1:1.1) on calcium silicate production at a calcination temperature of 1000 ℃. Furthermore, calcium silicate is reduced to nano-size by adopting various milling time intervals (0, 4, 6, 8, 10 h) using a planetary ball mill. The results indicate that the mix proportion of 1:1 yields the highest amount of calcium silicate, and a milling time of 6 h yields the smallest particle size. The confirmation of calcium silicate (in this case, wollastonite) formation is verified using various analysis techniques, such as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and Scanning electron microscopy (SEM). Particle size distribution analysis and transmission electron microscopy (TEM) show that the mean particle size is less than 100 nm, and Brunauer–Emmett–Teller (BET) analysis reveals a surface area of 6 m2/g. The study highlights that the solid-state reaction method might be a promising approach for effectively treating waste materials. [ABSTRACT FROM AUTHOR]

Background and Aim: A wide range of dental materials have incorporated the concept of nanotechnology into their composition to enhance their physical and antimicrobial properties. In this pretext, silver nanoparticles (AgNPs) are among the most commonly used nanoparticles which are exceptionally noteworthy for their role in medical applications as an antibacterial agent. Another essential, desirable physical characteristic of all endodontic cements is their radiopacity, while in similar context, various radiopacifying agents such as bismuth oxide, barium sulfate, and even AgNPs have been incorporated in endodontic sealers to enhance their physical properties. The aim of the present study was to assess whether the incorporation of AgNPs and 10% bismuth oxide imparted the required radiopacity to the novel cement material (Nano CS) as per the requirement and standards laid by the International Organization for Standardization (ISO) guidelines and whether it complied with the ISO 6876:2001 specifications to achieve the necessary norms. Materials and Methods: The structural characteristics of the novel cement material (Nano CS) were observed using energy-dispersive X-ray analysis under a Zeiss Gemini 500 Field Emission Scanning Electron Microscope, while radiopacity of the test material (Nano CS) was assessed with the help of an aluminum (Al) step‐wedge using a nondestructive testing method following ISO guidelines. The optical density of the test material (Nano CS) was tested with the specimens of mineral trioxide aggregate (MTA) as the standard cement material along with the specimens of enamel and dentin that were 1 mm thick, and Al of appropriate thickness with the desired and equivalent radiopacity. Results: The findings of the present study suggested MTA to have higher radiopacity index equivalent to 4.56 ± 0.00 mm thickness of Al when compared to the test material (Nano CS) (2.78 ± 0.01 mm thickness of Al) and enamel (4.09 ± 0.01 mm thickness of Al) and dentin (2.01 ± 0.01 mm thickness of Al) specimens. Furthermore, the radiopacity index of test material (Nano CS) was found to be more when compared to dentin, though, less when compared to the enamel specimens with the results being statistically highly significant (P < 0.001). Conclusion: The addition of nanosilver and bismuth oxide to the test material (Nano CS) imparted characteristic radiopacity, though the required specifications laid down by the ISO standards were not achieved. Increasing the concentration of the additives used might be considered to bring in the required radiopacity without having a significant impact on the physical and biological properties of the test material (Nano CS) intended to be used for endodontic applications.

This study investigates the potential of calcium silicates (CS) synthesised from granite and marble waste as an adsorbent for sustainable waste management. A simple and chemical-free synthesis method was adopted in the preparation of CS. X-ray diffraction, scanning electron microscopy, zeta potential and particle size distribution techniques were used for the characterisation of CS. As Pb(II) and its derivatives from various industrial effluents exercise significant negative impact on the environment and human health, an attempt was made to remove Pb(II) by adsorption process. Batch experiments were conducted to evaluate the feasibility of removal of pb(II) using CS. The zeta potential value of −83.7 mV and the mean particle size of 916 nm for the prepared CS can enhance adsorption process. The analysis of various adsorption kinetic models reveals that pseudo second order kinetic model exhibited a favourable level of agreement with kinetic data (R2 = 0.999). Response surface methodology utilising central composite design was employed to evaluate various process parameters, such as initial Pb(II) concentration, pH, adsorbent dosage and sonication time on adsorption process. Results from 30 experimental runs performed in accordance with model recommendations concluded that the effect of selected parameters with an R2 value of 0.937 was adequate for the current study. In order to assess the goodness of fit and statistical significance of the model's performance, ANOVA and Lack of Fit (LOF) tests were conducted. The findings show that Pb(II) can be easily removed from the aqueous solutions using CS as an adsorbent under optimal experimental conditions of 100 mg/l Pb(II) initial concentration, 2 g/l adsorbent dosage, pH of 8 and a sonication time of 45 min. [ABSTRACT FROM AUTHOR]

Aim: The aim of this study was to evaluate the flow, solubility, and pH of AH Plus sealer, Bio-C Sealer, and Cerafill RCS. Methods: The selected sealer materials were mixed according to instructions of the manufacturers under aseptic conditions. For flow and solubility, the recommendations of ISO 6876/2012 and ANSI/ADA 2000 standards were followed (n = 10). Additionally, pH changes during the setting of selected sealers (n = 10) were measured at regular intervals of time using a digital pH meter. Data were statistically analyzed using Kruskal–Wallis ANOVA test with post hoc Dunn’s test. Results: The flow of Bio-C Sealer was significantly higher than Cerafill RCS (P < 0.05). Among the sealers, Bio-C Sealer exhibited the highest solubility, whereas AH Plus exhibited the least solubility. Calcium silicate-based sealers showed higher pH than resin-based sealer. Conclusion: The results of the present study indicate that resin-based sealer and calcium silicate-based sealers have acceptable flow, whereas calcium silicate sealers exhibited high solubility and an alkaline pH.

Abstract Background Bioceramic-based root canal sealers are increasingly important in root canal treatment because of their biocompatible properties. This study aimed to evaluate postobturation pain incidence and intensity after root canal obturation with NeoSealer Flo, MTA-Bioseal, and GuttaFlow bioseal calcium silicate-based sealers and AH Plus epoxy resin-based sealer in patients with asymptomatic irreversible pulpitis or necrotic pulp with chronic apical periodontitis. Methods A total of 120 participants with single-rooted teeth were included and randomly divided into four groups according to the root canal sealer used (n = 30). The patients were subsequently categorised based on the pulp vitality (vital or nonvital) in each group (n = 15) and all teeth were obturated in a single-visit. The postobturation pain scores were recorded on a visual analogue scale (VAS) at 6 h, 24 h, 48 h, 72 h, 7 d and 30 d. Moreover, analgesic intake was also noted at 24 h and 48 h. The Mann–Whitney U test, Kruskal‒Wallis H test, Friedman test, and Spearman’s correlation test were used, and a p value 24 h > 48 h ≈ 72 h ≈ 7 d ≈ 30 d time intervals for both pulp status in each root canal sealer. A significant decrease in the VAS score was observed for all sealers from 6 h to 48 h (p 0.05). Nonvital cases had lower VAS scores at all time intervals. Analgesic intake was greater in the first 24 h (p

Abstract Background This clinical study aims to compare postoperative pain after single-visit root canal treatment of teeth with asymptomatic apical periodontitis using epoxy-resin-based AH Plus and calcium silicate-based Endosequence BC sealers with or without sonic activation. Methods This study included 72 individuals with one first or second mandibular premolar tooth with asymptomatic apical periodontitis. They were randomly divided into four groups according to the root canal sealer (AH Plus or Endosequence BC) and activation protocol (sonic activation or non-activation) (n = 18). The participants were ask to rate their postoperative pain intensity on a NRS scale as none, minimal, moderate, or severe after 24 h, 48 h, 72 h, and 7 days following treatment. Patients were also asked to record the number of prescribed analgesic medication tablets (400 mg of ibuprofen) taken. Statistical analysis were performed using the Mann-Whitney U test, the Friedman test, the Spearman’s correlation test, the Chi-square test. Significance level was established at 0.05. Results There was no statistically significant difference in postoperative pain scores or analgesic intake between AH Plus and Endosequence BC sealers regardless of the activation protocol (p > 0.05). There was no statistically significant difference in postoperative pain scores between the sonic activation and non-activation groups (p > 0.05). A weak positive correlation was detected between Endoactivator sonic activation and sealer extrusion (r = 0.36). Conclusions AH Plus and EndoSequence BC root canal sealers showed similar results in terms of prevalance and intensity of postoperative pain. Sonic activation and non-activation groups had similar postoperative pain scores. Clinical trial registration The study was retrospectively registered with ClinicalTrials.gov (NCT06403293). Date of Registration: 07/05/24.

Abstract Calcium silicate ceramic is a promising bioceramic for various biomedical applications, but its high biodegradation rate and low strength restrict its clinical utility. As a result, the study devised an innovative solution to address these issues by utilizing the titanium aluminum carbide phase, potentially for the first time in biological applications, in conjugation with hydroxyapatite. Then, using powder metallurgy technology, they added these phases to calcium silicate to create nanocomposites. After soaking in simulated body fluid for ten days, the produced nanocomposites were assessed for bioactivity and biodegradability using scanning electron microscopy, inductively coupled plasma-atomic emission spectroscopy, and weight loss assays. Their electrical and dielectric properties were also measured before and after soaking in the simulated body fluid solution. Furthermore, the tribo-mechanical properties of all sintered samples were measured. Interestingly, adding 40% hydroxyapatite nanoparticles to calcium silicate reduced the porosity from 12 to 6%. However, adding five vol% of the titanium aluminum carbide phase to the same sample increased the porosity to 8%. Importantly, these recorded percentages of porosity were comparable to those of compact bone porosity, which range from 5 to 13%. The addition of hydroxyapatite and titanium aluminum carbide phase significantly improved the rapid biodegradation of calcium silicate, albeit with a slight decrease in its bioactive properties, as evidenced by the incomplete surface coverage of the samples with the hydroxyapatite layer in the scanning electron microscopy images. The electrical properties of the nanocomposites were better with the addition of hydroxyapatite and titanium aluminum carbide phase, which helped the bone heal faster. The addition of a titanium aluminum carbide phase significantly improved the mechanical properties of the resulting nanocomposites. For example, the calculated values for compressive strength of all examined samples were 131, 115, 105, 147, and 135 MPa. Based on the results, the prepared samples can be used in orthopaedic and dental applications.

Abstract Background This study aimed to compare the marginal adaptation of a single customized gutta percha cone with calcium silicate-based sealer versus mineral trioxide aggregate (MTA) and Biodentine apical plugs in simulated immature permanent teeth. Methods Thirty-nine extracted human maxillary anterior teeth were selected, prepared to simulate immature permanent teeth with an apical diameter 1.1 mm, placed in moist foam and divided into three groups. Group 1: Obturation with a single customized gutta percha cone and calcium silicate sealer. Group 2: MTA apical plug. Group 3: Biodentine apical plug. After incubation, teeth were horizontally sectioned at 1 mm and 3 mm from the apex and marginal adaptation was evaluated using scanning electron microscope (SEM). Results Biodentine showed the least mean gap size at both 1 and 3 mm from the apex with no statistically significant differences compared to MTA (p > 0.05). The single customized cone with calcium silicate based sealer showed the greatest mean gap size at both 1 and 3 mm from the apex with a statistically significant difference compared to the other groups (p

The aim of this study was to evaluate the effects of mechanized final irrigation protocols (XPE, XP-Endo Finisher; XPC, XP-Clean; and ECL, Easy Clean) compared to PUI (passive ultrasonic irrigation) on the debris incidence and open dentinal tubules, and their effects on the adhesion interface after 48 h and 6 months. One hundred twenty maxillary central incisors were submitted to chemical–mechanical preparation using a rotary instrument and 2.5% sodium hypochlorite. Specimens were distributed in 4 groups (n = 30) in accordance with the mechanized final irrigation protocol: XPE, XPC, ECL, or PUI. Forty specimens (n = 10/group) were submitted to SEM analysis to evaluate the residue incidence and dentin open tubules. The other specimens were obturated using Bio-C Sealer and submitted to push-out bond strength and adhesive failure mode evaluations in the cervical, middle, and apical thirds after 48 h or 6 months (n = 10/group). Only in the apical third, ECL presented the highest residue incidence and fewer open dentinal tubules when compared to the XPE, XPC, and PUI groups (p < 0.05). In the cervical and middle root thirds, no significant differences were observed regardless of the group evaluated (p > 0.05). After 48 h, ECL resulted in the lowest bond strength only in the apical third (p < 0.05), while the XPE, XPC, and PUI groups remained similar in the cervical and middle thirds (p > 0.05). At 6 months, all groups showed lower bond strength values regardless of the root third evaluated, but ECL showed the lowest bond strength in the apical and middle root thirds when compared to the other groups (p < 0.05). The ECL protocol did not provide adequate residue removal on the apical radicular third and negatively affected the longevity of endodontic obturation using a calcium silicate-based sealer. [ABSTRACT FROM AUTHOR]

Production of Portland cement consumes a huge amount of silica mineral. The present work aims to introduce desert sand to replace the natural mineral to achieve green manufacturing of silicate cement clinker, as well as further decreasing the synthesis temperature and increasing the production efficiency. The effects of desert sand, industrial quartz and high‐purity quartz as raw materials, and Al2O3 and Fe2O3 as additives on phase transformation and microstructure of calcium silicate powder were comparably investigated using X‐ray diffractometer, differential scanning calorimeter, and scanning electron microscopy. Results show that the main phases of all samples with different raw materials and additives were 3CaO·SiO2 (C3S) and 2CaO·SiO2 (C2S). In which, the samples prepared using desert sand had the lowest phase transformation temperature for C2S and C3S. When adding Al2O3 and Fe2O3, the phase transformation temperatures of C2S and C3S further decreased, which were 1032.5°C and 1270.9°C, respectively, meanwhile phase content reached the maximum (C2S of 65.6 wt.% and C3S of 61.2 wt.%). In addition, the most uniformly and finely distributed crystalline phases formed at an optimal calcination temperature of 1350°C. This is beneficial for reducing production costs, saving mineral resources, and promoting the sustainable development of the cement industry. [ABSTRACT FROM AUTHOR]

Background: Bioceramic-based root canal sealers are increasingly important in root canal treatment because of their biocompatible properties. This study aimed to evaluate postobturation pain incidence and intensity after root canal obturation with NeoSealer Flo, MTA-Bioseal, and GuttaFlow bioseal calcium silicate-based sealers and AH Plus epoxy resin-based sealer in patients with asymptomatic irreversible pulpitis or necrotic pulp with chronic apical periodontitis. Methods: A total of 120 participants with single-rooted teeth were included and randomly divided into four groups according to the root canal sealer used (n = 30). The patients were subsequently categorised based on the pulp vitality (vital or nonvital) in each group (n = 15) and all teeth were obturated in a single-visit. The postobturation pain scores were recorded on a visual analogue scale (VAS) at 6 h, 24 h, 48 h, 72 h, 7 d and 30 d. Moreover, analgesic intake was also noted at 24 h and 48 h. The Mann–Whitney U test, Kruskal‒Wallis H test, Friedman test, and Spearman's correlation test were used, and a p value < 0.05 was considered significant. Results: VAS scores were highest for the 6 h > 24 h > 48 h ≈ 72 h ≈ 7 d ≈ 30 d time intervals for both pulp status in each root canal sealer. A significant decrease in the VAS score was observed for all sealers from 6 h to 48 h (p 0.05). Nonvital cases had lower VAS scores at all time intervals. Analgesic intake was greater in the first 24 h (p < 0.05) in vital cases (p < 0.05) and also in females than males. Conclusion: The level of pain experienced after obturation was similar in patients with different pulp status for all the root canal sealers. Analgesic intake was greater in vital cases and females within 24 h. Trial registration: ClinicalTrial.gov, NCT06515756, record date: 2024-07-17, retrospectively registered. [ABSTRACT FROM AUTHOR]

Cellular ceramics are highly valued for their exceptional mechanical properties and low density, making them suitable for applications like thermal insulation, impact absorption, and biomedical implants. However, high-temperature sintering required for densification can cause issues like shrinkage, loss of precision, and degradation of functional additives. This work reports a novel approach for fabricating 3D printed cellular ceramics under ambient conditions using CO 2 -cured γ-C 2 S ink via direct ink writing. The printed green body is converted into a dense matrix of CaCO 3 and silica gel upon exposure to a CO 2 -rich environment without high temperature sintering. The non-sintered cellular ceramics achieve high compressive and specific strength comparable to sintered counterparts, with the square-shaped structure exhibiting the best performance of 104 and 171 MPa in terms of in-plane and out-of-plane compressive strength, respectively. Further, the in-situ analysis reveals distinct deformation modes and crack propagation patterns across structures, providing insights into structure-property relationships to guide the design of high-strength non-sintered cellular ceramics. [ABSTRACT FROM AUTHOR]

Polysulfone (PSF) medical waste can be effectively repurposed due to its excellent mechanical properties. Due to the increasing need for load-bearing bone implants, it is crucial to prioritize the development of biocompatible polymer–matrix composites. Calcium silicate (CaSi), known for its osteogenesis and antibacterial properties, is widely used in medical applications. In this study, recycled PSF plastics in fiber or nanoparticle forms and commercial PSF products were used to create PSF-based composites filled with three different amounts (10, 20, and 30 vol%) of CaSi. The green compact was heat-treated at various temperatures. Experimental results showed that the mechanical interlocking of the PSF matrix and CaSi filler occurred due to the liquefaction of PSF fibers or nanoparticles during heat treatment. When the composite contained 20% CaSi, the obtained three-point bending strength exceeded 60 MPa, falling within the reported strength of compact bone. There was a concurrent improvement in the biocompatibility and antibacterial activity of the PSF-based composites with the increasing amount of CaSi. Considering their mechanical properties and antibacterial activity, the 20% CaSi-containing PSF-based composites treated at 240 °C emerged as a promising candidate for bone implant applications. This study demonstrated the feasibility of upcycling medical waste such as PSF as a matrix, opening doors for its potential usage in the medical field. [ABSTRACT FROM AUTHOR]

Background: Different pulp capping materials have different origins and compositions, require different preparations, and may vary in their bioactive properties. Aim: The purpose of this study was to evaluate the antibacterial activity, biocompatibility, and mineralization‐inducing potential of calcium silicate‐based pulp capping materials. Design: Six contemporary calcium silicate‐based cements, ProRoot MTA, MTA Angelus, Biodentine, EndoSequence, NeoMTA 2, and NeoPutty, were evaluated. The antibacterial effects of these materials against Streptococcus mutans UA159 and Enterococcus faecalis ATCC 29212 were determined by the agar diffusion assay and the direct culture test. The biocompatibility and mineralization‐inducing potential of these materials in preodontoblastic 17IIA11 cells were evaluated by the MTT assay and by Alizarin Red S staining, respectively. Results and Conclusion: In agar diffusion test, only Biodentine showed distinct antibacterial effects against S. mutans. All the tested materials, however, showed antibacterial effects against S. mutans and E. faecalis in the direct culture test, with Biodentine showing the strongest growth inhibition against both S. mutans and E. faecalis. All the tested materials showed acceptable biocompatibility and mineralization‐supporting potential in our experimental conditions. In summary, ProRoot MTA, MTA Angelus, Biodentine, EndoSequence, NeoMTA 2, and NeoPutty demonstrated acceptable in vitro antimicrobial, biocompatible, and mineralization‐supporting properties. [ABSTRACT FROM AUTHOR]

Background: This clinical study aims to compare postoperative pain after single-visit root canal treatment of teeth with asymptomatic apical periodontitis using epoxy-resin-based AH Plus and calcium silicate-based Endosequence BC sealers with or without sonic activation. Methods: This study included 72 individuals with one first or second mandibular premolar tooth with asymptomatic apical periodontitis. They were randomly divided into four groups according to the root canal sealer (AH Plus or Endosequence BC) and activation protocol (sonic activation or non-activation) (n = 18). The participants were ask to rate their postoperative pain intensity on a NRS scale as none, minimal, moderate, or severe after 24 h, 48 h, 72 h, and 7 days following treatment. Patients were also asked to record the number of prescribed analgesic medication tablets (400 mg of ibuprofen) taken. Statistical analysis were performed using the Mann-Whitney U test, the Friedman test, the Spearman's correlation test, the Chi-square test. Significance level was established at 0.05. Results: There was no statistically significant difference in postoperative pain scores or analgesic intake between AH Plus and Endosequence BC sealers regardless of the activation protocol (p > 0.05). There was no statistically significant difference in postoperative pain scores between the sonic activation and non-activation groups (p > 0.05). A weak positive correlation was detected between Endoactivator sonic activation and sealer extrusion (r = 0.36). Conclusions: AH Plus and EndoSequence BC root canal sealers showed similar results in terms of prevalance and intensity of postoperative pain. Sonic activation and non-activation groups had similar postoperative pain scores. Clinical trial registration: The study was retrospectively registered with ClinicalTrials.gov (NCT06403293). Date of Registration: 07/05/24. [ABSTRACT FROM AUTHOR]

To evaluate experimental dimethacrylate-based materials containing calcium orthophosphates or calcium silicate particles in terms of their optical, mechanical and Ca2+ release behaviour. Dicalcium phosphate dihydrate (DCPD), hydroxyapatite (HAp), beta-tricalcium phosphate (β-TCP) or calcium silicate (CaSi) particles were added to a photocurable BisGMA/TEGDMA resin (1:1 in mols) at a 30 vol% fraction. Materials containing silanized or non-silanized barium glass particles were used as controls. Degree of conversion (DC) at the top and base of 2-mm thick specimens was determined by ATR-FTIR spectroscopy (n = 5). Translucency parameter (TP) and transmittance (%T) were determined using a spectrophotometer (n = 3). Biaxial flexural strength (BFS) and flexural modulus (FM) were determined by biaxial flexural testing after 24 h storage in water (n = 10). Ca2+ release in water was determined during 28 days by inductively coupled plasma optical emission spectrometry (n = 3). Statistical analysis was performed using ANOVA/Tukey test (DC: two-way; TP, %T; BFS and FM: one-way; Ca2+ release: repeated measures two-way, α = 5 %). Results: CaSi and β-TCP particles drastically reduced DC at 2 mm, TP and %T (p < 0.001). Compared to both controls, all Ca2+-releasing materials presented lower BFS (p < 0.001) and only the material with DCPD showed significantly lower FM (p < 0.05). The material containing CaSi presented the highest Ca2+ release, while among materials formulated with calcium orthophosphates the use of DCPD resulted in the highest release (p < 0.001). CaSi particles allowed the highest Ca2+ release. Notwithstanding, the use of DCPD resulted in a material with the best compromise between optical behaviour, DC, strength and Ca2+ release. • The addition of β-TCP and CaSi reduced degree of conversion, %T and translucency. • All particles negatively affected biaxial flexural strength. • The addition of DCPD reduced the flexural modulus in relation to the control. • The addition CaSi resulted in the greater release of Ca2+. [ABSTRACT FROM AUTHOR]

Copyright of Science of Sintering is the property of National Library of Serbia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background and Aim: A wide range of dental materials have incorporated the concept of nanotechnology into their composition to enhance their physical and antimicrobial properties. In this pretext, silver nanoparticles (AgNPs) are among the most commonly used nanoparticles which are exceptionally noteworthy for their role in medical applications as an antibacterial agent. Another essential, desirable physical characteristic of all endodontic cements is their radiopacity, while in similar context, various radiopacifying agents such as bismuth oxide, barium sulfate, and even AgNPs have been incorporated in endodontic sealers to enhance their physical properties. The aim of the present study was to assess whether the incorporation of AgNPs and 10% bismuth oxide imparted the required radiopacity to the novel cement material (Nano CS) as per the requirement and standards laid by the International Organization for Standardization (ISO) guidelines and whether it complied with the ISO 6876:2001 specifications to achieve the necessary norms. Materials and Methods: The structural characteristics of the novel cement material (Nano CS) were observed using energy-dispersive X-ray analysis under a Zeiss Gemini 500 Field Emission Scanning Electron Microscope, while radiopacity of the test material (Nano CS) was assessed with the help of an aluminum (Al) step‐wedge using a nondestructive testing method following ISO guidelines. The optical density of the test material (Nano CS) was tested with the specimens of mineral trioxide aggregate (MTA) as the standard cement material along with the specimens of enamel and dentin that were 1 mm thick, and Al of appropriate thickness with the desired and equivalent radiopacity. Results: The findings of the present study suggested MTA to have higher radiopacity index equivalent to 4.56 ± 0.00 mm thickness of Al when compared to the test material (Nano CS) (2.78 ± 0.01 mm thickness of Al) and enamel (4.09 ± 0.01 mm thickness of Al) and dentin (2.01 ± 0.01 mm thickness of Al) specimens. Furthermore, the radiopacity index of test material (Nano CS) was found to be more when compared to dentin, though, less when compared to the enamel specimens with the results being statistically highly significant (P < 0.001). Conclusion: The addition of nanosilver and bismuth oxide to the test material (Nano CS) imparted characteristic radiopacity, though the required specifications laid down by the ISO standards were not achieved. Increasing the concentration of the additives used might be considered to bring in the required radiopacity without having a significant impact on the physical and biological properties of the test material (Nano CS) intended to be used for endodontic applications. [ABSTRACT FROM AUTHOR]

Aim: The aim of this study was to evaluate the flow, solubility, and pH of AH Plus sealer, Bio-C Sealer, and Cerafill RCS. Methods: The selected sealer materials were mixed according to instructions of the manufacturers under aseptic conditions. For flow and solubility, the recommendations of ISO 6876/2012 and ANSI/ADA 2000 standards were followed (n = 10). Additionally, pH changes during the setting of selected sealers (n = 10) were measured at regular intervals of time using a digital pH meter. Data were statistically analyzed using Kruskal–Wallis ANOVA test with post hoc Dunn's test. Results: The flow of Bio-C Sealer was significantly higher than Cerafill RCS (P < 0.05). Among the sealers, Bio-C Sealer exhibited the highest solubility, whereas AH Plus exhibited the least solubility. Calcium silicate-based sealers showed higher pH than resin-based sealer. Conclusion: The results of the present study indicate that resin-based sealer and calcium silicate-based sealers have acceptable flow, whereas calcium silicate sealers exhibited high solubility and an alkaline pH. [ABSTRACT FROM AUTHOR]

The azalea lace bug (ALB), Stephanitis pyrioides (Scott) (Hemiptera: Tingidae), is a pest of azaleas and rhododendrons. The application of silicon (Si) to plants has been shown to accumulate in other plants and enhance defense to other plant pests. We evaluated whether Si applications decreased ALB infestation on rhododendron leaves and increased Si accumulation in leaves. Potted plants were treated with 4 or 8 weekly applications of calcium silicate and calcium carbonate (calcium control, Ca) via foliar or soil application. In 3 out of 4 choice studies, plants treated with calcium silicate or calcium carbonate had less frass deposition and oviposition by ALB compared to controls, but treated plants did not consistently have fewer ALB adults. Leaf damage was quantified in one study and leaves with more frass as an indicator of feeding had more visible damage. In no-choice studies, there were no differences between treatments in one study, but oviposition was greater on foliar/soil Si-treated plants than controls in another study. Since rhododendron aphids (Illinoia lambersi) appeared in the greenhouse during or after studies, we compared their colonization on previously treated rhododendrons. Infestation of new leaf rosettes or random leaves by I. lambersi was lower on plants sprayed with foliar silicon or calcium applied via soil in 2 studies. Treated rhododendrons did not accumulate extra Si or Ca in leaves compared to controls. In general, silicon or calcium application protected rhododendrons from ALB oviposition and aphid colonization in free-choice conditions, and may be part of an integrated pest management program. [ABSTRACT FROM AUTHOR]

Introduction: Calcium silicate-based sealers are an alternative to be used into root canal, mainly to their biological properties. However, some biological parameters need to be determined in an in vivo animal research model. So, the aim of the present study was to evaluate in vivo the tissue biocompatibility of a calcium silicate-based sealer (EndoSequence BC Sealer) and an epoxy resin-based sealer (AH-Plus). Materials and Methods: Polyethylene tubes were filled with freshly mixed sealers and implanted in connective subcutaneous tissue of 25 rats (5/euthanasia day) (Rattus norvegicus albinus). Empty tubes were used as controls and no tubes as sham. Histopathological (hematoxylin eosin) and histochemical (Picrosirius red) examinations were conducted at 3, 7, 15, 30 and 60 days (five rats/day) after the implantation procedure (n=5/group). The type/intensity of inflammation and collagenesis was analyzed statistically with Friedman or Kruskal-Wallis/Dunn tests (P<0.05). Results: The profile of inflammation induced by AH-Plus (Median=2, Range=2-3) was significantly greater than that by Endosequence BC Sealer (Median=1, Range=1-1) during the 15-day experimentation period (P=0.018). After 30 days, both materials produced similar tissue reaction (P>0.05). AH-Plus and Endosequence BC Sealer (Median=2, Range=1-2) induced a high level of fibrosis after 60-day than control (Median=1, Range=1-1) and sham (Median=0, Range=0-0) groups (P<0.001) of fibrosis based in type I collagen increase (P=0.025 and P=0.021, respectively). Tissue necrosis was not observed and the bioceramic sealer showed significant signs of endocytosed (Median=1, Range=1-1) material after 7 days than other groups (Median=0, Range=0-0) (P<0.05). The calcium silicate-based sealer induced tissue repair faster than the epoxy resin-based sealer tested. However, both materials showed adequate biocompatibility and tolerance by subcutaneous tissues, with few differences in inflammatory profiles, formation of granulation tissue, and collagenesis. Conclusions: It may be concluded that calcium silicate-based sealer (EndoSequence BC Sealer) and an epoxy resin-based sealer (AH-Plus) presented suitable biocompatibility. [ABSTRACT FROM AUTHOR]

Copyright of Oilfield Chemistry is the property of Sichuan University, Oilfield Chemistry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: This study aimed to compare the marginal adaptation of a single customized gutta percha cone with calcium silicate-based sealer versus mineral trioxide aggregate (MTA) and Biodentine apical plugs in simulated immature permanent teeth. Methods: Thirty-nine extracted human maxillary anterior teeth were selected, prepared to simulate immature permanent teeth with an apical diameter 1.1 mm, placed in moist foam and divided into three groups. Group 1: Obturation with a single customized gutta percha cone and calcium silicate sealer. Group 2: MTA apical plug. Group 3: Biodentine apical plug. After incubation, teeth were horizontally sectioned at 1 mm and 3 mm from the apex and marginal adaptation was evaluated using scanning electron microscope (SEM). Results: Biodentine showed the least mean gap size at both 1 and 3 mm from the apex with no statistically significant differences compared to MTA (p > 0.05). The single customized cone with calcium silicate based sealer showed the greatest mean gap size at both 1 and 3 mm from the apex with a statistically significant difference compared to the other groups (p < 0.001). Conclusion: Biodentine and MTA apical plugs provide a significantly better marginal adaptation to the dentinal walls than a single customized gutta percha cone with calcium silicate based sealer in simulated immature permanent teeth. [ABSTRACT FROM AUTHOR]

Roses, due to their delicacy and short vase life, require special care; however, the use of calcium can help improve their growth, quality, and longevity. This study was designed and conducted as a completely randomized design to investigate the effect of three calcium sources (distilled water as a control, calcium silicate, and calcium amino chelate) at a concentration of 160 ppm on the growth and quality of Rosa 'Samurai' in a hydroponic system. The results showed that foliar application of calcium silicate and calcium amino chelate improved growth traits, including stem diameter, fresh stem weight, flower diameter, flower count, and leaf area compared to the control. Calcium silicate and calcium amino chelate increased the photosynthesis rate by 36.94% and 30.78%, respectively. The highest anthocyanin content (8.39) was observed in the calcium amino chelate treatment, while the calcium concentrations in leaves and petals increased by 47.91% and 51.67%, respectively, in the calcium silicate treatment compared to the control. Calcium amino chelate also resulted in a 47.86% increase in root calcium concentration and a 3.3-day increase in vase life compared to the control. Additionally, calcium silicate increased iron concentration by 11.34%, manganese by 91.74%, and leaf nitrogen by 5.88%, while calcium amino chelate increased leaf and root copper concentrations by 4% and 25.41%, phosphorus concentration in leaves by 25.90%, and root potassium concentration by 74.84%. These results demonstrate the significant role of calcium, particularly calcium amino chelate, in enhancing nutrient absorption and improving the growth and quality of rose flowers. [ABSTRACT FROM AUTHOR]

Calcium-based materials, such as calcium carbonate, calcium phosphate, and calcium silicate, have attracted significant attention in biomedical research, owing to their unique physicochemical properties and versatile applications. The distinctive characteristics of these materials, including their inherent biocompatibility and tunable structures, hold significant promise for applications in bone regeneration and tissue engineering. This review explores the biomedical applications of calcium-containing materials, particularly for bone regeneration. Their remarkable biocompatibility, tunable nanostructures, and multifaceted functionalities make them pivotal for advancing regenerative medicine, drug delivery system, and biomimetic scaffold applications. The evolving landscape of biomedical research continues to uncover new possibilities, positioning calcium-based materials as key contributors to the next generation of innovative biomaterial scaffolds. [ABSTRACT FROM AUTHOR]

Porous calcium silicate ceramic has been prepared by ultrafast high‐temperature process in several minutes. The effect of sintering current and starch content on the phase composition, pore structure, compressive strength, and air permeability have been investigated. Homogeneous pores can be formed during the fast decomposition of calcium silicate hydrate, calcium carbonate, and starch. The optimized composition shows the high compressive strength (over 7 MPa), porosity (60.8%), and air permeability (379.4 L/m2 s). Results showed that the ultrafast high‐temperature sintering method can achieve homogenous porous structure in much shorter time than conventional furnace sintering. [ABSTRACT FROM AUTHOR]

Neutron and x‐ray diffraction measurements have been performed on CaO–MgO–Al2O3–SiO2 (CMAS) glasses doped with NiO–FeXO at room temperature, along with x‐ray measurements on aerodynamically levitated liquids at ≥2000 K. The disordered structures have been modeled using empirical potential structure refinement to investigate the relation between the aluminosilicate network and the modifying cations. The SiO4 and AlO4 tetrahedra are found to have wider Si–O and Al–O bond distance distributions in the glass, and the first Ca–On coordination shell is highly distorted, redistributing different populations of long and short bonds between the liquid and the glass. The addition of Fe and Ni at low aluminosilicate content increases the number of free oxygens not bonded to AlO4 or SiO4. Mg–O and Fe–O are both found to be predominantly fourfold and fivefold in the liquid and glassy states. Despite these low coordination numbers, their bond angle distributions indicate that they are predominantly in nontetrahedral‐type geometries, with ferrous and ferric iron possessing similar coordination environments. The Ca–O and Mg–O average coordination numbers and enthalpies of solution are consistent with their higher reactivity within relatively acidic aluminosilicate melts. [ABSTRACT FROM AUTHOR]

Premixed calcium silicate-based materials have recently been developed and are recommended for a wide range of endodontic procedures, including vital pulp therapy. This study investigated the in vitro biocompatibility and pro-mineralization effect and in vivo reparative dentin formation of EndoSequence Root Repair Material, EndoSequence BCRRM, Bio-C Repair, and Well-pulp PT. Both fresh and set extracts had no detrimental effect on the growth of human dental pulp stem cells. The fresh extracts had a higher calcium concentration than the set extracts and induced considerably greater mineralized nodule formation. EndoSequence Root Repair Material had the longest setting time, whereas Bio-C Repair had the shortest. When these materials were applied to exposed rat molar pulps, mineralized tissue deposition was found at the exposure sites after 2 weeks. These results indicate that the premixed calcium silicate-based materials tested could have positive benefits for direct pulp capping procedures. [ABSTRACT FROM AUTHOR]

Purpose: To compare the radiopacity of two calcium silicate-based sealers (NeoSealer Flo and Sure-Seal Root), a calcium silicate particle-containing silicone (GuttaFlow Bioseal), and an epoxy resin-based root canal sealer (AH Plus Jet) using digital radiography. Methods: Five samples (1 ± 0.1 mm height, 10 ± 0.1 mm diameter) were prepared for each material tested. After the samples were completely set, digital radiographs of the samples and an aluminum stepwedge were taken. The mean gray values of each sample and aluminum step were calculated using the ImageJ program, and the values were converted into equivalent aluminum thicknesses. Kruskal- Wallis and post-hoc Dunn tests were used to analyze the data. Results: The radiopacity values of NeoSealer Flo (4.9 mm Al), GuttaFlow Bioseal (4.84 mm Al), and Sure- Seal Root (4.36 mm Al) were significantly lower than those of AH Plus Jet (10.83 mm Al) (p < 0.0001), but there were no significant differences among the three (p > 0.05). Conclusion: Although the calcium silicate-based and calcium silicate particle-containing siliconebased sealers exhibited lower radiopacity than the resin-based sealer, the radiopacity values of all root canal sealers tested met the minimum Al standard values advised by the International Organization for Standardization and the American National Standards Institute/American Dental Association. [ABSTRACT FROM AUTHOR]

The decision making process for managing irreversible pulpitis in permanent teeth often involves choosing between pulpotomy and root canal treatment, with clinicians exercising discretion. This systematic review and meta-analysis aim to evaluate the efficacy of calcium silicate based pulpotomy in treating symptomatic permanent teeth with irreversible pulpitis. A comprehensive search across PubMed, Google Scholar, and SCOPUS databases yielded 482 articles. From this pool, 169 articles underwent further scrutiny. Following data filtration, presentation, and analysis, a meta-analysis was conducted using RevMan 5 software. Eleven articles were ultimately selected based on predefined inclusion and exclusion criteria. This meta-analysis revealed that calcium silicate based pulpotomy, employed in treating irreversible pulpitis cases, exhibited notable success rates ranging from 75% to 90% over a period of 15 years across most of the included studies. The majority of studies integrated into this systematic review reported successful outcomes for teeth with symptomatic irreversible pulpitis undergoing pulpotomy with the utilization of calcium silicate. Nonetheless, further research is necessary to delineate the precise roles of calcium silicate based pulpotomy in managing this condition. [ABSTRACT FROM AUTHOR]

Based on an earlier study, a mimic composition 38SiO2–60CaO-0.5Dy2O3-0.2Na2O–1MgO-0.1Al2O3-0.1TiO2-0.1Fe2O3 (wt.%) are synthesized by solid-state reaction method. The structural, morphological, and optical properties of the synthesized sample presented in this study are compared with the corresponding properties of a similar sample derived from rice husk ash and eggshell powder as resources. The conventional chemicals derived sample are multi-phasic, containing β−Ca2SiO4, γ−Ca2SiO4, Ca3Si2O7, and CaFeO3 phases, and the CaO/SiO2 ratio was believed to be affecting the room temperature stabilization of the β phase. Field emission scanning electron microscopy images showed that twinned particles, cracks, and edge dislocations were present in the synthesized sample. The presence of precipitates in the micrographs indicated that Dy ions were segregated on the surface of the sample. The optical bandgap of the samples was 3.7 eV, as calculated by diffuse reflectance spectroscopy. The photoluminescence emission spectrum contained characteristic emission peaks of the Dy3+ ions. Apart from these, additional peaks were observed due to the titanium ions present in the sample. Understanding the properties of calcium silicates containing multiple dopant ions is important to develop a white light-emitting diode. The properties of the mineral-derived sample are compared to those of the agro-food waste-derived similar sample.

The decision making process for managing irreversible pulpitis in permanent teeth often involves choosing between pulpotomy and root canal treatment, with clinicians exercising discretion. This systematic review and meta-analysis aim to evaluate the efficacy of calcium silicate based pulpotomy in treating symptomatic permanent teeth with irreversible pulpitis. A comprehensive search across PubMed, Google Scholar, and SCOPUS databases yielded 482 articles. From this pool, 169 articles underwent further scrutiny. Following data filtration, presentation, and analysis, a meta-analysis was conducted using RevMan 5 software. Eleven articles were ultimately selected based on predefined inclusion and exclusion criteria. This meta-analysis revealed that calcium silicate based pulpotomy, employed in treating irreversible pulpitis cases, exhibited notable success rates ranging from 75% to 90% over a period of 15 years across most of the included studies. The majority of studies integrated into this systematic review reported successful outcomes for teeth with symptomatic irreversible pulpitis undergoing pulpotomy with the utilization of calcium silicate. Nonetheless, further research is necessary to delineate the precise roles of calcium silicate based pulpotomy in managing this condition.

Objectives: This study aimed to investigate the in vitro effects of root canal filling and repair paste (nRoot BP) on human dental pulp stem cells (hDPSCs). Methods: The effects of nRoot BP and iRoot BP Plus on the adhesion, proliferation, migration, and differentiation of hDPSCs were examined in vitro for 72 hours. The adhesion of cells was observed using immunofluorescence rhodamine ghost pen cyclic peptide staining and scanning electron microscopy (SEM). Cell density and changes in migration area were measured under a fluorescence inverted microscope. Fluorescent quantitative PCR was performed to detect genes related to odontogenesis and osteogenesis. Results: Cells adhering to the surfaces of nRoot BP and iRoot BP Plus exhibited similar irregular polygonal morphologies, with cells extending irregular pseudopods to adhere to the materials. CCK-8 results indicated that the density of living cells for nRoot BP and iRoot BP Plus was lower than that of the blank control group at 3 and 5 days of culture. There was no significant difference in cell migration between the groups (P > .05). The migration ability of iRoot BP Plus and nRoot BP was similar to that of the control group. Both nRoot BP and iRoot BP Plus increased the expression of the RUNX2 gene, but there was no significant difference between the groups (P < .05). Furthermore, both nRoot BP and iRoot BP Plus downregulated the expression of the DSPP gene, with no significant difference between them (P > .05). Conclusions: nRoot BP exhibited a slight inhibition of hDPSC proliferation but did not affect the adhesion and migration of hDPSCs. The impact of nRoot BP on the osteogenic and odontogenic differentiation of hDPSCs was similar to that of iRoot BP Plus.

Context: An acidic hydrogen potential (pH) in an inflammatory condition in the periapical tissues may affect the properties of repair bioceramic cement. Aims: The aim of this study was to evaluate the effect of pH on the volumetric change of the ready-to-use bioceramic NeoPUTTY (NP) compared to the powder/liquid MTA Repair HP (MTAHP) after immersion in butyric acid (BA, pH 4.1) or phosphate-buffered saline (PBS, pH 7.35). Subjects and Methods: Dentin tubes filled with NP or MTAHP were scanned in micro-computed tomography (micro-CT) after 24 h. Then, the specimens were immersed in 1.5 mL of BA: NP/BA, MTAHP/BA or PBS: NP/PBS, MTAHP/PBS. After 7 days, new micro-CT scans were performed. The percentage of volumetric change (extremities and internal part) of the materials was assessed. Statistical Analysis Used: ANOVA/Tukey and Kruskal-Wallis tests were performed (α =0.05). Results: All materials showed a volumetric decrease after immersion in BA or PBS at the extremities in contact with the solutions. MTAHP/BA showed the highest volumetric loss. There was no difference in the volumetric change when the internal part of the materials was evaluated. Conclusions: An acid pH negatively affects the volumetric stability of MTAHP. Low values of volumetric change were demonstrated for NP in both immersion environments. [ABSTRACT FROM AUTHOR]

(1) Background: The aim of this study was to compare the cytotoxicity of selected resin-modified materials used in direct contact with the dental pulp (TheraCal LC, TheraCal PT, and ApaCal ART) with calcium silicate cement (Biodentine). (2) Methods: The mouse fibroblast Balb/3T3 cell line and the extracts of tested materials in four concentrations were used for the testing. An MTT assay was performed in three independent experiments with six replicates for each concentration of tested material. The cell viability (%) and cytotoxicity were expressed (cytotoxic effect is considered in cases where the cell viability is lower than 70%). The mean of the cell viability and the standard deviation were expressed for each material at all concentrations. ANOVA and Dunnet's post hoc tests were used for the statistical analysis. All of these tests were performed at the 0.05 significance level. (3) Results: At all concentrations, the cell viability was statistically significantly lower (p ≤ 0.002) for all tested materials compared to Biodentine. ApaCal ART showed a high level of cytotoxicity at all concentrations (cell viability lower than 47.71%, p < 0.0001). The same result was found for TheraCal LC at concentrations of 100%, 50% and 25% and TheraCal PT at concentrations of 100% and 50%. TheraCal LC at a 10% concentration (cell viability 68.18%) and TheraCal PT at a 25% concentration (cell viability 60.63%) indicated potential cytotoxicity. TheraCal PT at a 10% concentration was not found to be cytotoxic (cell viability 79.18%, p = 0.095). (4) Conclusion: The resin-modified calcium silicate and calcium phosphate materials showed higher cytotoxic potential, so they should be used with caution when in direct contact with the dental pulp. [ABSTRACT FROM AUTHOR]