1. Proteomic and carbonylation profile analysis of rat skeletal muscles following acute swimming exercise
- Author
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Provvidenza Maria Abruzzo, Francesca Magherini, Alessandra Modesti, Tania Fiaschi, Marina Marini, Laura Pietrovito, Fabio Esposito, Tania Gamberi, Luca Bini, Massimo Gulisano, Magherini F, Gamberi T, Pietrovito L, Fiaschi T, Bini L, Esposito L, Marini M, Abruzzo PM, Gulisano M, and Modesti A
- Subjects
Male ,Proteomics ,Anatomy and Physiology ,Proteome ,Muscle Proteins ,lcsh:Medicine ,Hindlimb ,medicine.disease_cause ,Mass Spectrometry ,Protein Carbonylation ,Lipid peroxidation ,chemistry.chemical_compound ,Molecular Cell Biology ,esercizio fisico ,lcsh:Science ,Musculoskeletal System ,Cellular Stress Responses ,proteomica ,Spectrometric Identification of Proteins ,Multidisciplinary ,muscolo scheletrico ,Animal Models ,musculoskeletal system ,Cellular Structures ,medicine.anatomical_structure ,Muscle ,Medicine ,Research Article ,Signal Transduction ,medicine.medical_specialty ,animal structures ,carbonilazione delle proteine ,Oxidative phosphorylation ,Biology ,Model Organisms ,Physical Conditioning, Animal ,Internal medicine ,medicine ,Animals ,Sports and Exercise Medicine ,Muscle, Skeletal ,Protein Interactions ,Swimming ,Actin ,proteomics ,muscle ,protein carbonylation ,lcsh:R ,Skeletal muscle ,Rats ,Endocrinology ,chemistry ,Rat ,lcsh:Q ,Lipid Peroxidation ,Protein Abundance ,Oxidative stress - Abstract
Previous studies by us and other groups characterized protein expression variation following long-term moderate training, whereas the effects of single bursts of exercise are less known. Making use of a proteomic approach, we investigated the effects of acute swimming exercise (ASE) on protein expression and carbonylation patterns in two hind limb muscles: the Extensor Digitorum Longus (EDL) and the Soleus, mostly composed of fast-twitch and slow-twitch fibres, respectively. Carbonylation is one of the most common oxidative modifications of proteins and a marker of oxidative stress. In fact, several studies suggest that physical activity and the consequent increase in oxygen consumption can lead to increase in reactive oxygen and nitrogen species (RONS) production, hence the interest in examining the impact of RONS on skeletal muscle proteins following ASE. Results indicate that protein expression is unaffected by ASE in both muscle types. Unexpectedly, the protein carbonylation level was reduced following ASE. In particular, the analysis found 31 and 5 spots, in Soleus and EDL muscles respectively, whose carbonylation is reduced after ASE. Lipid peroxidation levels in Soleus were markedly reduced as well. Most of the decarbonylated proteins are involved either in the regulation of muscle contractions or in the regulation of energy metabolism. A number of hypotheses may be advanced to account for such results, which will be addressed in future studies.
- Published
- 2013