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Hypoxia complicates various complications of pregnancy, somatic and infectious diseases of the mother, her harmful habits. The cardiovascular system is one of the most vulnerable under such conditions. The aim of the study was to detect morphological features of CD16 macrophages and smooth myocytes (SMC) of the pulmonary artery (PA) of fetuses and newborns from mothers with experimental chronic intrauterine hypoxia (CIH) on the basis of a complex pathomorphological study. The study was conducted on laboratory WAG line rats. All material was divided into a control group (18 cases), and a group of off springs who suffered from chronic oxygen deficiency (16 cases). Morphological investigation included macroscopic, histologic (hematoxylin and eosin staining), immunohistochemical (study of macrophages CD16 and smooth myocytes) methods, followed by statistical processing of the received data. Macroscopic study using a magnifier (×3, 8 diopters) of both groups PA demonstrated its wall elasticity, smooth intima with whitish-grayish color. Microscopically, with hematoxylin and eosin staining all three layers of the vessel are determined: the inner (tunica intima), medium (tunica media) and external (tunica adventitia). The smooth muscles cells were observed diffuse evenly with MCA to Monoclonal Anti-Human Smooth Muscle Actin in the sub-endothelial layer, the medium layer. They had oblong shape, with moderate intensity expressed cytoplasm marker. The density of their location in wall thickness of the control group PA was 28.20±0.63 cells in the field of view (×1000). The density of SMC location in wall thickness of the CIH group PA was 20.00±0.35 cells in the field of view (×1000). In addition to intima and medium layers, SMC were still located in the adventitious vessels walls. The number of macrophages CD16 in the investigated vessel wall of control group was determined; their placement density was 26.35±1.42 cells in the field of view (×600). The placement density of macro

Hypoxia complicates various complications of pregnancy, somatic and infectious diseases of the mother, her harmful habits. The cardiovascular system is one of the most vulnerable under such conditions. The aim of the study was to detect morphological features of CD16 macrophages and smooth myocytes (SMC) of the pulmonary artery (PA) of fetuses and newborns from mothers with experimental chronic intrauterine hypoxia (CIH) on the basis of a complex pathomorphological study. The study was conducted on laboratory WAG line rats. All material was divided into a control group (18 cases), and a group of off springs who suffered from chronic oxygen deficiency (16 cases). Morphological investigation included macroscopic, histologic (hematoxylin and eosin staining), immunohistochemical (study of macrophages CD16 and smooth myocytes) methods, followed by statistical processing of the received data. Macroscopic study using a magnifier (×3, 8 diopters) of both groups PA demonstrated its wall elasticity, smooth intima with whitish-grayish color. Microscopically, with hematoxylin and eosin staining all three layers of the vessel are determined: the inner (tunica intima), medium (tunica media) and external (tunica adventitia). The smooth muscles cells were observed diffuse evenly with MCA to Monoclonal Anti-Human Smooth Muscle Actin in the sub-endothelial layer, the medium layer. They had oblong shape, with moderate intensity expressed cytoplasm marker. The density of their location in wall thickness of the control group PA was 28.20±0.63 cells in the field of view (×1000). The density of SMC location in wall thickness of the CIH group PA was 20.00±0.35 cells in the field of view (×1000). In addition to intima and medium layers, SMC were still located in the adventitious vessels walls. The number of macrophages CD16 in the investigated vessel wall of control group was determined; their placement density was 26.35±1.42 cells in the field of view (×600). The placement density of macro

Hypoxia complicates various complications of pregnancy, somatic and infectious diseases of the mother, her harmful habits. The cardiovascular system is one of the most vulnerable under such conditions. The aim of the study was to detect morphological features of CD16 macrophages and smooth myocytes (SMC) of the pulmonary artery (PA) of fetuses and newborns from mothers with experimental chronic intrauterine hypoxia (CIH) on the basis of a complex pathomorphological study. The study was conducted on laboratory WAG line rats. All material was divided into a control group (18 cases), and a group of off springs who suffered from chronic oxygen deficiency (16 cases). Morphological investigation included macroscopic, histologic (hematoxylin and eosin staining), immunohistochemical (study of macrophages CD16 and smooth myocytes) methods, followed by statistical processing of the received data. Macroscopic study using a magnifier (×3, 8 diopters) of both groups PA demonstrated its wall elasticity, smooth intima with whitish-grayish color. Microscopically, with hematoxylin and eosin staining all three layers of the vessel are determined: the inner (tunica intima), medium (tunica media) and external (tunica adventitia). The smooth muscles cells were observed diffuse evenly with MCA to Monoclonal Anti-Human Smooth Muscle Actin in the sub-endothelial layer, the medium layer. They had oblong shape, with moderate intensity expressed cytoplasm marker. The density of their location in wall thickness of the control group PA was 28.20±0.63 cells in the field of view (×1000). The density of SMC location in wall thickness of the CIH group PA was 20.00±0.35 cells in the field of view (×1000). In addition to intima and medium layers, SMC were still located in the adventitious vessels walls. The number of macrophages CD16 in the investigated vessel wall of control group was determined; their placement density was 26.35±1.42 cells in the field of view (×600). The placement density of macro

Hypoxia complicates various complications of pregnancy, somatic and infectious diseases of the mother, her harmful habits. The cardiovascular system is one of the most vulnerable under such conditions. The aim of the study was to detect morphological features of CD16 macrophages and smooth myocytes (SMC) of the pulmonary artery (PA) of fetuses and newborns from mothers with experimental chronic intrauterine hypoxia (CIH) on the basis of a complex pathomorphological study. The study was conducted on laboratory WAG line rats. All material was divided into a control group (18 cases), and a group of off springs who suffered from chronic oxygen deficiency (16 cases). Morphological investigation included macroscopic, histologic (hematoxylin and eosin staining), immunohistochemical (study of macrophages CD16 and smooth myocytes) methods, followed by statistical processing of the received data. Macroscopic study using a magnifier (×3, 8 diopters) of both groups PA demonstrated its wall elasticity, smooth intima with whitish-grayish color. Microscopically, with hematoxylin and eosin staining all three layers of the vessel are determined: the inner (tunica intima), medium (tunica media) and external (tunica adventitia). The smooth muscles cells were observed diffuse evenly with MCA to Monoclonal Anti-Human Smooth Muscle Actin in the sub-endothelial layer, the medium layer. They had oblong shape, with moderate intensity expressed cytoplasm marker. The density of their location in wall thickness of the control group PA was 28.20±0.63 cells in the field of view (×1000). The density of SMC location in wall thickness of the CIH group PA was 20.00±0.35 cells in the field of view (×1000). In addition to intima and medium layers, SMC were still located in the adventitious vessels walls. The number of macrophages CD16 in the investigated vessel wall of control group was determined; their placement density was 26.35±1.42 cells in the field of view (×600). The placement density of macro

Хронічна внутрішньоутробна гіпоксія займає одне з перших місць серед чинників, що ушкоджують органи сечовидільної системи плодів та новонароджених. Метою даної роботи є виявлення морфофункціональних особливостей нирок, сечоводів і сечового міхура у плодів та новонароджених, які зазнали впливу хронічної внутрішньоутробної гіпоксії. Матеріалом даного дослідження є тканина нирок, сечоводів і сечового міхура плодів та новонароджених, яку вивчали за допомогу різних гістологічних та гістохімічних методів забарвлення. В ході проведеного авторами дослідження в органах сечовидільної системи плодів та новонароджених, які зазнали впливу хронічної внутрішньоутробної гіпоксії, виявлено різні морфофункціональні зміни, які в подальшому онтогенезі можуть призвести до розвитку різної патології даної системи у таких дітей., Хроническая внутриутробная гипоксия занимает одно из первых мест среди факторов, повреждающих органы мочевыделительной системы плодов и новорожденных. Целью данной работы явилось выявление морфофункциональных особенностей почек, мочеточников и мочевого пузыря у плодов и новорожденных, подвергшихся влиянию хронической внутриутробной гипоксии. Материалом данного исследования послужила ткань почек, мочеточников и мочевого пузыря плодов и новорожденных, которую изучали с помощь различных гистологических и гистохимических методов окраски. В ходе проведенного авторами исследования в органах мочевыделительной системы плодов и новорожденных, подвергшихся влиянию хронической внутриутробной гипоксии, выявлены различные морфофункциональные изменения, которые в дальнейшем онтогенезе могут привести к развитию различной патологии данной системы у таких детей., Chronic intrauterine hypoxia is one of the main factors that affects the urinary system organs of fetuses and newborns. The purpose of this study was to identify the morphofunctional features of kidneys, ureters and urinary bladder in fetuses and newborns exposed to the influence of chronic intrauterine hypoxia. The material of this study was the tissue of kidneys, ureters and urinary bladder of fetuses and newborns, which was studied using different histological and histochemical staining methods. In the study conducted by the authors in organs of urinary system of fetuses and newborn, exposed to chronic intrauterine hypoxia, identified various morphological changes, which can lead to the development of various pathologies of such system in these children in future ontogenesis.