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After a century since the erection of the genus Manipuria from India, its type species M. dohertyi Jacoby was discovered in Yunnan Province of China. A new Manipuria species, M. yuaesp. nov., is described from Tibet and Yunnan, China. The new species differs from M. dohertyi by its larger size, unicolored elytra, and absence of a tooth-like prolongation in front of the mandible. Additional data is provided for M. dohertyi based on new material from China.

Intracellular bacteria of the genus Wolbachia (alpha-Proteobacteria) are the most widespread endosymbionts of insects. Host infection is usually associated with alterations in reproduction, such as cytoplasmic incompatibility, the induction of parthenogenesis and offspring sex ratio bias: all phenomena that may influence host speciation. In the present study, by using well-established molecular tools, we investigated the presence of Wolbachia in leaf beetles of the genus Crioceris and their host plants, which are various species of Asparagus. Multilocus sequence typing of bacterial genes showed that despite their occurrence in the same habitat and feeding on the same plant, two species of Crioceris, C. quinquepunctata and C. quatuordecimpunctata, are infected by two different strains of Wolbachia. C. asparagi, C. paracenthesis and C. duodecimpunctata, which are sympatric with the infected species, do not harbour the bacterium. Interestingly, DNA of Wolbachia was detected in host plant tissues that are exploited by the beetles, providing evidence for the horizontal transmission of the bacterium between beetles and their host plants. Moreover, Wolbachia was detected in species of Crioceris that are not closely related.

Crioceris paracenthesis (Linnaeus) MALLORCA���Art�� 10.03.1982, 1 ex.; Banyalbufar, 0 5.10.1983, 1 ex.; Bunyola: torrent, 24.09.1982, 1 ex.; Can Picafort, 0 4.07.1984, 2 exx.; Capdepera 10.09.1983, 7 exx.; Esporles 26.05.1984, 1 ex.; ibid 23.04.1988, 1 ex.; ibid 0 2.05.1999, 1 ex.; ibid 0 9.10.1999, 2 exx.; ibid 14.10.1999, 2 exx.; ibid 31.10.1999, 2 exx.; ibid 0 1.11.1999, 1 ex.; ibid 14.09.2002, 2 exx.; ibid 12.10.2003, 1 ex.; Petra 0 6.06.1989 (leg. R. Beenen); Son Serra de Marina: torrent Na Borges 10.06.1991, 2 exx. On Asparagus acutifolius L. and A. albus L. (Liliaceae). This is the most abundant species of Crioceris in Mallorca, although as the previous one it seems to have become scarcer recently. MENORCA���Although we have not collected any individual from this island, this species should be probably there because it has been found in Mallorca and Eivissa. EIVISSA: Santa Eul��ria 21.10.1996, 1 ex. (Sacar��s & Petitpierre 1999)., Published as part of Petitpierre, Eduard, Sacar��s, Antoni & Jurado-Rivera, Jos�� A., 2017, Updated checklist of Balearic leaf beetles (Coleoptera: Chrysomelidae), pp. 151-177 in Zootaxa 4272 (2) on pages 152-153, DOI: 10.11646/zootaxa.4272.2.1, http://zenodo.org/record/583820, {"references":["Sacares, A. & Petitpierre, E. (1999) Noves cites de Chrysomelidae (Coleoptera) d'Eivissa i Formentera (Illes Pitiuses). Bolleti de la Societat d'Historia Natural de les Balears, 42, 33 - 37."]}

Crioceris asparagi (Linnaeus) MALLORCA���Port d���Alc��dia: foothills behind 20.10.1990, 1 ex. (Whitehead 1993 sub C. asparagi var. campestris Linnaeus). A very rare species in the island contrary to its abundance in the Iberian Peninsula., Published as part of Petitpierre, Eduard, Sacar��s, Antoni & Jurado-Rivera, Jos�� A., 2017, Updated checklist of Balearic leaf beetles (Coleoptera: Chrysomelidae), pp. 151-177 in Zootaxa 4272 (2) on page 152, DOI: 10.11646/zootaxa.4272.2.1, http://zenodo.org/record/583820, {"references":["Whitehead, A. (1993) Observations on the Coleoptera of Mallorca, Balearic Islands. Bolleti de la Societat d'Historia Natural de les Balears, 36, 45 - 56."]}

Crioceris macilenta Weise MALLORCA���Banyalbufar 11.10.1982, 2 exx.; ibid 0 5.10.1983, 8 exx.; Felanitx: castell de Santueri 13.04.1986, 1 ex.; Son Serra de Marina: torrent Na Borges 17.04.1989, 2 exx.; Esporles 31.10.1999, 1 ex., on Asparagus albus L.; ibid 14.04.2002, 1 ex. on A. acutifolius L.; ibid 20.04.2002, 6 exx., on A. acutifolius; ibid 28.04.2002, 2 exx., on A. acutifolius; ibid 20.04.2003, 1 ex.; ibid 0 1.05.2003, 1 ex.; ibid 0 3.04.2004, 2 exx.; ibid 0 1.05.2004, 3 exx.; Palma: Campus UIB 0 8.11.2003, 1 ex.Nevertheless, it has not been captured in the last ten years, which probably means that it is becoming rarer for unknown reasons. MENORCA���Ma�� 0 6.1971, 1 ex. (leg. P. Orom��), Published as part of Petitpierre, Eduard, Sacar��s, Antoni & Jurado-Rivera, Jos�� A., 2017, Updated checklist of Balearic leaf beetles (Coleoptera: Chrysomelidae), pp. 151-177 in Zootaxa 4272 (2) on page 152, DOI: 10.11646/zootaxa.4272.2.1, http://zenodo.org/record/583820

Based on a single well-preserved specimen from Eocene Baltic amber, Lilioceris groehni sp. n. is described and illustrated using phase-contrast X-ray synchrotron microtomography. It is the first described species of Criocerinae (Coleoptera: Chrysomelidae) from Baltic amber. A check-list of fossil Criocerinae is provided. Placement of Crioceris pristiana (Germar, 1813) is discussed, this species is removed from Criocerinae and placed in Coleoptera incertae sedis.

Phylogenetic relationships among major groups of Criocerinae were reconstructed using molecular data (mitochondrial cytochrome oxidase I and 12S rDNA, and nuclear histone 3). The monophyly of Criocerinae was consistently and robustly supported. The Lema group including Lema, Oulema and Neolema was recovered as a clade, with the latter two genera imbedded within Lema. The Lilioceris group was placed as the sister taxon of the Lema group, and the genus Crioceris was identified as the sister taxon of the Lilioceris + Lema groups. The monophyly and/or validity of Mecoprosopus Chûjô, 1951 and the subgenera Lema, Petauristes Latreille, 1829, Quasilema Monrós, 1960, Microlema Pic, 1932, and Bradyceris Chûjô, 1951 were not confirmed. The monophyly of the subgenus Lema except for the type species L. cyanea was supported by molecular and morphological data, and we termed it the cyanella clade. The present molecular phylogeny was compared with previous concepts with respect to the validity of each genus/subgenus. A revision of several genera is necessary. Based on the phylogenic result, the character evolution of the reproductive organs was analyzed. The ancestral states of this character system were parsimoniously reconstructed. Various shapes of the spermatheca were observed in the subfamily. A convoluted spermatheca evolved once, and reversals to the ancestral state took place several times independently. An elongation of a part of the intromittent organ also occurred several times independently. The length of the male and female reproductive ducts, which are in physical contact during copulation, showed a tight positive correlation even after removing phylogenetic effects. This strongly suggests coevolution between the male and female genital length.

Phylogenetic relationships among major groups of Criocerinae were reconstructed using molecular data (mitochondrial cytochrome oxidase I and 12S rDNA, and nuclear histone 3). The monophyly of Criocerinae was consistently and robustly supported. The Lema group including Lema, Oulema and Neolema was recovered as a clade, with the latter two genera imbedded within Lema. The Lilioceris group was placed as the sister taxon of the Lema group, and the genus Crioceris was identified as the sister taxon of the Lilioceris + Lema groups. The monophyly and/or validity of Mecoprosopus Chûjô, 1951 and the subgenera Lema, Petauristes Latreille, 1829, Quasilema Monrós, 1960, Microlema Pic, 1932, and Bradyceris Chûjô, 1951 were not confirmed. The monophyly of the subgenus Lema except for the type species L. cyanea was supported by molecular and morphological data, and we termed it the cyanella clade. The present molecular phylogeny was compared with previous concepts with respect to the validity of each genus/subgenus. A revision of several genera is necessary. Based on the phylogenic result, the character evolution of the reproductive organs was analyzed. The ancestral states of this character system were parsimoniously reconstructed. Various shapes of the spermatheca were observed in the subfamily. A convoluted spermatheca evolved once, and reversals to the ancestral state took place several times independently. An elongation of a part of the intromittent organ also occurred several times independently. The length of the male and female reproductive ducts, which are in physical contact during copulation, showed a tight positive correlation even after removing phylogenetic effects. This strongly suggests coevolution between the male and female genital length.

12. Crioceris quatuordecimpunctata Aedeagus (Fig. 76): Median lobe stubby, apical half of median lobe enlarged; ventral edge of median orifice rounded; median foramen expanding apical half of ventral surface of median lobe. Tegmen forming clubshaped lobe in lateral view. Male internal reproductive system (Fig. 104): Testis large, almost oval and constricted at middle, yellowish orange in color. Accessory glands elliptic or pear-like; of a pair of accessory glands, shorter gland about 1 / 3 times and longer gland half length to maximal width of apparent testis. Lateral ejaculatory duct short, a little over 1 / 3 times as long as maximal width of apparent testis; common ejaculatory duct slightly tapering toward base, almost equal length to maximal width of apparent testis. Female internal reproductive system (Figs. 119, 120 & 139): Eggs yellowish orange in color. Spermathecal organ connected to bursa copulatrix ventro-apically. Spermathecal capsule hook-shaped, tapering toward apex, yellowish brown in color; proximal part of spermathecal capsule tubular, somewhat constricted at middle in some individuals; spermathecal wall thick; spermathecal duct relatively short, 1.3���1.5 times as long as maximal length of spermathecal capsule; spermathecal gland tubular, a little longer than maximal length of spermathecal capsule., Published as part of Matsumura, Yoko & Suzuki, Kunio, 2008, Comparative morphology of internal reproductive systems in leaf beetles of the Donaciinae and Criocerinae (Coleoptera: Chrysomelidae) and its implication for the phylogeny, pp. 1-32 in Zootaxa 1845 on pages 19-22, DOI: 10.5281/zenodo.183289