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14 results on '"da Fonseca-Martins, Alessandra Marcia"'

1. B-1 lymphocytes are able to produce IL-10, but is not pathogenic during Leishmania (Leishmania) amazonensis infection

Author

Firmino-Cruz, Luan, Ramos, Tadeu Diniz, da Fonseca-Martins, Alessandra Marcia, Oliveira-Maciel, Diogo, Oliveira-Silva, Gabriel, dos Santos, Júlio Souza, Cavazzoni, Cecília, Morrot, Alexandre, Gomes, Daniel Claudio Oliveira, Vale, André Macedo, Decoté-Ricardo, Debora, Freire-de-Lima, Celio G., and de Matos Guedes, Herbert Leonel

Published
2020
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2. Combined therapy with adipose tissue-derived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis

Author

Ramos, Tadeu Diniz, Silva, Johnatas Dutra, da Fonseca-Martins, Alessandra Marcia, da Silveira Pratti, Juliana Elena, Firmino-Cruz, Luan, Maciel-Oliveira, Diogo, Dos-Santos, Julio Souza, Tenorio, João Ivo Nunes, de Araujo, Almair Ferreira, Freire-de-Lima, Célio Geraldo, Diaz, Bruno Lourenço, Cruz, Fernanda Ferreira, Rocco, Patricia Rieken Macedo, and de Matos Guedes, Herbert Leonel

Published
2020
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3. Intradermal Immunization of SARS-CoV-2 Original Strain Trimeric Spike Protein Associated to CpG and AddaS03 Adjuvants, but Not MPL, Provide Strong Humoral and Cellular Response in Mice

Author

Firmino-Cruz, Luan, primary, dos-Santos, Júlio Souza, additional, da Fonseca-Martins, Alessandra Marcia, additional, Oliveira-Maciel, Diogo, additional, Guadagnini-Perez, Gustavo, additional, Roncaglia-Pereira, Victor A., additional, Dumard, Carlos H., additional, Guedes-da-Silva, Francisca H., additional, Vicente Santos, Ana C., additional, Alvim, Renata G. F., additional, Lima, Tulio M., additional, Marsili, Federico F., additional, Abreu, Daniel P. B., additional, Rossi-Bergmann, Bartira, additional, Vale, Andre M., additional, Filardy, Alessandra D’Almeida, additional, Silva, Jerson Lima, additional, de Oliveira, Andrea Cheble, additional, Gomes, Andre M. O., additional, and de Matos Guedes, Herbert Leonel, additional

Published
2022
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4. Immunogenicity of SARS-CoV-2 Trimeric Spike Protein Associated to Poly(I:C) Plus Alum

Author

dos-Santos, Júlio Souza, primary, Firmino-Cruz, Luan, additional, da Fonseca-Martins, Alessandra Marcia, additional, Oliveira-Maciel, Diogo, additional, Perez, Gustavo Guadagnini, additional, Roncaglia-Pereira, Victor A., additional, Dumard, Carlos H., additional, Guedes-da-Silva, Francisca H., additional, Santos, Ana C. Vicente, additional, Leandro, Monique dos Santos, additional, Ferreira, Jesuino Rafael Machado, additional, Guimarães-Pinto, Kamila, additional, Conde, Luciana, additional, Rodrigues, Danielle A. S., additional, Silva, Marcus Vinicius de Mattos, additional, Alvim, Renata G. F., additional, Lima, Tulio M., additional, Marsili, Federico F., additional, Abreu, Daniel P. B., additional, Ferreira Jr., Orlando C., additional, Mohana Borges, Ronaldo da Silva, additional, Tanuri, Amilcar, additional, Souza, Thiago Moreno L., additional, Rossi-Bergmann, Bartira, additional, Vale, André M., additional, Silva, Jerson Lima, additional, de Oliveira, Andréa Cheble, additional, Filardy, Alessandra D’Almeida, additional, Gomes, Andre M. O., additional, and de Matos Guedes, Herbert Leonel, additional

Published
2022
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5. Subtilisin of Leishmania amazonensis as Potential Druggable Target: Subcellular Localization, In Vitro Leishmanicidal Activity and Molecular Docking of PF-429242, a Subtilisin Inhibitor

Author

Gomes, Pollyanna Stephanie, primary, Carneiro, Monique Pacheco Duarte, additional, Machado, Patrícia de Almeida, additional, de Andrade-Neto, Valter Viana, additional, da Fonseca-Martins, Alessandra Marcia, additional, Goundry, Amy, additional, Pereira da Silva, João Vitor Marques, additional, Gomes, Daniel Claudio Oliveira, additional, Lima, Ana Paula Cabral de Araujo, additional, Ennes-Vidal, Vítor, additional, Sodero, Ana Carolina Rennó, additional, De-Simone, Salvatore Giovanni, additional, and de Matos Guedes, Herbert L., additional

Published
2022
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6. MPLA and AddaVax® Adjuvants Fail to Promote Intramuscular LaAg Vaccine Protectiveness against Experimental Cutaneous Leishmaniasis

Author

Oliveira-Maciel, Diogo, primary, dos-Santos, Júlio Souza, additional, Oliveira-Silva, Gabriel, additional, Mello, Mirian França de, additional, da Fonseca-Martins, Alessandra Marcia, additional, Carneiro, Monique Pacheco Duarte, additional, Ramos, Tadeu Diniz, additional, Firmino-Cruz, Luan, additional, Gomes, Daniel Claudio Oliveira, additional, Rossi-Bergmann, Bartira, additional, and de Matos Guedes, Herbert Leonel, additional

Published
2021
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7. Dietary Vitamin D3 Deficiency Increases Resistance to Leishmania (Leishmania) amazonensis Infection in Mice

Author

Bezerra, Izabella Pereira da Silva, primary, Oliveira-Silva, Gabriel, additional, Braga, Danielle Sophia Ferreira Santos, additional, de Mello, Mirian França, additional, Pratti, Juliana Elena Silveira, additional, Pereira, Joyce Carvalho, additional, da Fonseca-Martins, Alessandra Marcia, additional, Firmino-Cruz, Luan, additional, Maciel-Oliveira, Diogo, additional, Ramos, Tadeu Diniz, additional, Vale, André Macedo, additional, Gomes, Daniel Claudio Oliveira, additional, Rossi-Bergmann, Bartira, additional, and de Matos Guedes, Herbert Leonel, additional

Published
2019
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8. The role of TLR9 on Leishmania amazonensis infection and its influence on intranasal LaAg vaccine efficacy

Author

Pratti, Juliana Elena Silveira, primary, da Fonseca Martins, Alessandra Marcia, additional, da Silva, Juliana Paiva, additional, Ramos, Tadeu Diniz, additional, Pereira, Joyce Carvalho, additional, Firmino-Cruz, Luan, additional, Oliveira-Maciel, Diogo, additional, Vieira, Thiago Soares de Souza, additional, Lacerda, Leandra Linhares, additional, Vale, Andre Macedo, additional, Freire-de-Lima, Celio G., additional, Gomes, Daniel C. Oliveira, additional, Saraiva, Elvira M., additional, Rossi-Bergmann, Bartira, additional, and de Matos Guedes, Herbert Leonel, additional

Published
2019
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9. Immunomodulating role of IL-10-producing B cells in Leishmania amazonensis infection

Author

Firmino-Cruz, Luan, primary, Ramos, Tadeu Diniz, additional, da Fonseca-Martins, Alessandra Marcia, additional, Maciel-Oliveira, Diogo, additional, Oliveira-Silva, Gabriel, additional, Pratti, Juliana Elena Silveira, additional, Cavazzoni, Cecília, additional, Chaves, Suzana Passos, additional, Oliveira Gomes, Daniel Claudio, additional, Morrot, Alexandre, additional, Freire-de-Lima, Leonardo, additional, Vale, André M., additional, Freire-de-Lima, Celio Geraldo, additional, Decote-Ricardo, Debora, additional, and de Matos Guedes, Herbert Leonel, additional

Published
2018
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10. Effects of Bone Marrow Mesenchymal Stromal Cell Therapy in Experimental Cutaneous Leishmaniasis in BALB/c Mice Induced by Leishmania amazonensis

Author

Pereira, Joyce Carvalho, primary, Ramos, Tadeu Diniz, additional, Silva, Johnatas Dutra, additional, de Mello, Mirian França, additional, Pratti, Juliana Elena Silveira, additional, da Fonseca-Martins, Alessandra Marcia, additional, Firmino-Cruz, Luan, additional, Kitoko, Jamil Zola, additional, Chaves, Suzana Passos, additional, Gomes, Daniel Claudio De Oliveira, additional, Diaz, Bruno Lourenço, additional, Rocco, Patricia R. M., additional, and de Matos Guedes, Herbert Leonel, additional

Published
2017
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11. Efficacy of intranasal LaAg vaccine against Leishmania amazonensis infection in partially resistant C57Bl/6 mice

Author

Pratti, Juliana Elena Silveira, primary, Ramos, Tadeu Diniz, additional, Pereira, Joyce Carvalho, additional, da Fonseca-Martins, Alessandra Marcia, additional, Maciel-Oliveira, Diogo, additional, Oliveira-Silva, Gabriel, additional, de Mello, Mirian França, additional, Chaves, Suzana Passos, additional, Gomes, Daniel Claudio Oliveira, additional, Diaz, Bruno Lourenço, additional, Rossi-Bergmann, Bartira, additional, and de Matos Guedes, Herbert Leonel, additional

Published
2016
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12. Efficacy of intranasal LaAg vaccine against Leishmania amazonensis infection in partially resistant C57Bl/6 mice.

Author

Silveira Pratti, Juliana Elena, Ramos, Tadeu Diniz, Pereira, Joyce Carvalho, da Fonseca-Martins, Alessandra Marcia, Maciel-Oliveira, Diogo, Oliveira-Silva, Gabriel, de Mello, Mirian França, Chaves, Suzana Passos, Oliveira Gomes, Daniel Claudio, Diaz, Bruno Lourenço, Rossi-Bergmann, Bartira, and de Matos Guedes, Herbert Leonel

Subjects
LEISHMANIASIS vaccines, ENZYME-linked immunosorbent assay, INTERLEUKIN-18, IMMUNOREGULATION, LABORATORY mice
Abstract

Background: We have previously demonstrated that intranasal vaccination of highly susceptible BALB/c mice with whole Leishmania amazonensis antigens (LaAg) leads to protection against murine cutaneous leishmaniasis. Here, we evaluate the response of partially resistant C57BL/6 mice to vaccination as a more representative experimental model of human cutaneous leishmaniasis. Methods: C57BL/6 mice from different animal facilities were infected with L. amazonensis (Josefa strain) to establish the profile of infection. Intranasal vaccination was performed before the infection challenge with two doses of 10 μg of LaAg alone or associated with the adjuvant ADDAVAX® by instillation in the nostrils. The lesion progression was measured with a dial caliper and the parasite load by limited dilution assay in the acute and chronic phases of infection. Cytokines were quantified by ELISA in the homogenates of infected footpads. Results: C57BL/6 mice from different animal facilities presented the same L. amazonensis infection profile, displaying a progressive acute phase followed by a controlled chronic phase. Parasites cultured in M199 and Schneider’s media were equally infective. Intranasal vaccination with LaAg led to milder acute and chronic phases of the disease. The mechanism of protection was associated with increased production of IFN-gamma in the infected tissue as measured in the acute phase. Association with the ADDAVAX® adjuvant did not improve the efficacy of intranasal LaAg vaccination. Rather, ADDAVAX® reduced vaccination efficacy. Conclusion: This study demonstrates that the efficacy of adjuvant-free intranasal vaccination with LaAg is extendable to the more resistant C57Bl/6 mouse model of infection with L. amazonensis, and is thus not exclusive to the susceptible BALB/c model. These results imply that mucosal immunomodulation by LaAg leads to peripheral protection irrespective of the genetic background of the host. [ABSTRACT FROM AUTHOR]

Published
2016
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13. MPLA and AddaVax ® Adjuvants Fail to Promote Intramuscular LaAg Vaccine Protectiveness against Experimental Cutaneous Leishmaniasis.

Author

Oliveira-Maciel, Diogo, dos-Santos, Júlio Souza, Oliveira-Silva, Gabriel, Mello, Mirian França de, da Fonseca-Martins, Alessandra Marcia, Carneiro, Monique Pacheco Duarte, Ramos, Tadeu Diniz, Firmino-Cruz, Luan, Gomes, Daniel Claudio Oliveira, Rossi-Bergmann, Bartira, and de Matos Guedes, Herbert Leonel

Subjects
CUTANEOUS leishmaniasis, LEISHMANIASIS, VACCINES, VACCINE effectiveness, IMMUNIZATION, LEISHMANIA
Abstract

There is so far no vaccine approved for human leishmaniasis, mainly because of the lack of appropriate adjuvants. This study aimed to evaluate in mice the capacity of a mixture of monophosphoryl lipid A (MPLA) and AddaVax® adjuvants in enhancing the efficacy of a Leishvacin®-like vaccine comprised of Leishmania amazonensis whole antigens (LaAg). For that, mice were immunized with LaAg plus MPLA/AddaVax® by the intramuscular route (i.m.) prior to challenge with 2 × 105 and 2 × 106 living parasites. Immunization with LaAg alone reduced the lesion growth of the 2 × 105-challenged mice only in the peak of infection, but that was not accompanied by reduced parasite load, and thus not considered protective. Mice given a 2 × 106 -challenge were not protected by LaAg. The association of LaAg with MPLA/AddaVax® was able to enhance the cutaneous hypersensitivity response compared with LaAg alone. Despite this, there was no difference in proliferative cell response to antigen ex vivo. Moreover, regardless of the parasite challenge, association of LaAg with MPL/AddaVax® did not significantly enhance protection in comparison with LaAg alone. This work demonstrated that MPL/AddaVax® is not effective in improving the efficacy of i.m. LaAg vaccine against cutaneous leishmaniasis. [ABSTRACT FROM AUTHOR]

Published
2021
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14. Characterization of Sv129 Mice as a Susceptible Model to Leishmania amazonensis .

Author

Dos-Santos JS, Firmino-Cruz L, Ramos TD, da Fonseca-Martins AM, Oliveira-Maciel D, De-Medeiros JVR, Chaves SP, Gomes DCO, and de Matos Guedes HL

Abstract

Leishmaniasis is a complex of neglected diseases caused by parasites of the genus Leishmania , such as Leishmania (Leishmania) amazonensis , the ethiologic agent of diffuse cutaneous leishmaniasis in Brazil. In this work, we investigated a new experimental model of infection for L. amazonensis : the Sv129 mouse. First, we subcutaneously infected Sv129 mice with 2 × 10 5 or 2 × 10 6 L. amazonensis parasites of the Josefa strain. A progressive lesion developed for both inoculation doses, showing that Sv129 mice are susceptible, independent of parasite dose. We next investigated the mechanisms associated with the pathogenesis of infection. We did not observe an increase of frequency of interferon-gamma (IFN- γ)-producing CD4 + and CD8 + T cells, a phenotype similar to that seen in BALB/c mice. There was an increased of frequency and number of IL-17-producing γδ (gamma-delta) T cells in infected Sv129 mice compared to naïve SV129 and an increased frequency of this population compared to infected BALB/c mice. In addition, Sv129 mice presented high levels of both IgG1 and IgG2a, suggesting a mixed Th1 and Th2 response with a skew toward IgG1 production based on IgG1/IgG2a ratio. Susceptibility of the Sv129 mice was further confirmed with the use of another strain of L. amazonensis , LTB0016. In this work, we characterized the Sv129 mice as a new model of susceptibility to Leishmania amazonensis infection, during infection there was controlled IFN-γ production by CD4 + or CD8 + T cells and induced IL-17 production by γδ T cells.

Published
2019
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