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Turfgrass anthracnose, caused by Colletotrichum cereale (ex. Colletotrichum graminicola), is an important disease of turf used on golf course putting greens. Recent management of the disease has become increasingly difficult, partly due to the possible development of practical resistance to the Qol fungicides. In all, 558 single-conidia isolates of C. cereale were collected from 10 California golf courses, 8 of which had been exposed to Qol fungicides and 2 where no fungicides had been used. Isolates were tested using a mycelial expansion assay on azoxystrobin- amended media. For the two nonexposed populations, in vitro 50% effective dose (ED50) values ranged from 0.0060 to 0.089 pg/mI. All isolates from the exposed populations could not be fully inhibited by doses of azoxystrobin as high as 8.0 pg/mI. A subset of these isolates were tested in vitro with the Qol fungicides pyraclostrobin and trifloxystrobin and found to be similar in re- sponse, indicating that these isolates were fully cross-resistant to all three fungicides. In green- house pot experiments, three isolates nonresponsive to Qol fungicides in vitro were not con- trolled by label rates of the fungicides. Spore germination assays also were examined; for 10 isolates identified as sensitive by mycelial expansion assays, ED50 values for axoystrobin ranged from 0.0040 to 0.0047 pg/mI; for 25 isolates identified as Qol-resistant, 93 to 100% of the co- nidia germinated at azoxystrobin concentrations as high as 8.0 pg/mI relative to the nonamended check treatments. Mitochondrial cytochrome b genes from a subset of IS isolates (12 resistant and 3 sensitive) were partially cloned and sequenced; all resistant isolates had an alanine substitution that corresponded to position 143 of the gene product. These results indicate that Qol resistance is present in California populations of C. cereale and is contributing to the difficulty in controlling this disease. [ABSTRACT FROM AUTHOR]