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3. Structural and Functional Alterations Caused by Aureobasidin A in Clinical Resistant Strains of Candida spp.

Author

Rollin-Pinheiro, Rodrigo, primary, de Moraes, Daniel Clemente, additional, Bayona-Pacheco, Brayan, additional, Curvelo, Jose Alexandre da Rocha, additional, dos Santos-Freitas, Giulia Maria Pires, additional, Xisto, Mariana Ingrid Dutra da Silva, additional, Borba-Santos, Luana Pereira, additional, Rozental, Sonia, additional, Ferreira-Pereira, Antonio, additional, and Barreto-Bergter, Eliana, additional

Published
2023
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5. Synthesis of Altissimacoumarin D and Other Prenylated Coumarins and Their Ability to Reverse the Multidrug Resistance Phenotype in Candida albicans

Author

Silva, Anna Claudia, primary, de Moraes, Daniel Clemente, additional, do Carmo, Denilson Costa, additional, Gomes, Giselle Cristina Casaes, additional, Ganesan, A., additional, Lopes, Rosangela Sabbatini Capella, additional, Ferreira-Pereira, Antonio, additional, and Lopes, Cláudio Cerqueira, additional

Published
2023
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6. β-Lapachone enhances the antifungal activity of fluconazole against a Pdr5p-mediated resistant Saccharomyces cerevisiaestrain

Author

de Moraes, Daniel Clemente, Cardoso, Karina Martins, Domingos, Levy Tenório Sousa, do Carmo Freire Ribeiro Pinto, Maria, Monteiro, Robson Q., and Ferreira-Pereira, Antônio

Abstract

Objectives: The aim of this study was to evaluate the ability of lapachones in disrupting the fungal multidrug resistance (MDR) phenotype, using a model of study which an azole-resistant Saccharomyces cerevisiaemutant strain that overexpresses the ATP-binding cassette (ABC) transporter Pdr5p. Methods: The evaluation of the antifungal activity of lapachones and their possible synergism with fluconazole against the mutant S. cerevisiaestrain was performed through broth microdilution and spot assays. Reactive oxygen species (ROS) and efflux pump activity were assessed by fluorometry. ATPase activity was evaluated by the Fiske and Subbarow method. The effect of β-lapachone on PDR5mRNA expression was assessed by RT-PCR. The release of hemoglobin was measured to evaluate the hemolytic activity of β-lapachone. Results: α-nor-Lapachone and β-lapachone inhibited S. cerevisiaegrowth at 100 μg/ml. Only β-lapachone enhanced the antifungal activity of fluconazole, and this combined action was inhibited by ascorbic acid. β-Lapachone induced the production of ROS, inhibited Pdr5p-mediated efflux, and impaired Pdr5p ATPase activity. Also, β-lapachone neither affected the expression of PDR5nor exerted hemolytic activity. Conclusions: Data obtained indicate that β-lapachone is able to inhibit the S. cerevisiaeefflux pump Pdr5p. Since this transporter is homologous to fungal ABC transporters, further studies employing clinical isolates that overexpress these proteins will be conducted to evaluate the effect of β-lapachone on pathogenic fungi.

Published
2024
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7. Dentine biomodification by sulphonamides pre-treatment: bond strength, proteolytic inhibition, and antimicrobial activity.

Author

Portela, Maristela Barbosa, Barboza, Caroliny Mello, da Silva, Eduardo Moreira, de Moraes, Daniel Clemente, Simão, Renata Antoun, de Souza, Clara Ribeiro, Cardoso, Verônica da Silva, Ferreira-Pereira, Antônio, Vermelho, Alane Beatriz, and Supuran, Claudiu T.

Subjects
DENTIN, BOND strengths, ANTI-infective agents, SULFONAMIDES, CARBONIC anhydrase inhibitors
Abstract

We evaluated the effects of dentine biomodification after pre-treatment with two sulphonamide carbonic anhydrase inhibitors (CAIs) of the N-[4-sulphamoylphenethylcarbamoyl]benzenesulphonamide type, investigating matrix metalloproteases activity, resin–dentine micro tensile bond strength, dentine surface wettability, and antimicrobial activities. Ninety-five sound-extracted human molars were selected for the study. Inhibitory effects were evaluated by gelatinase and collagenase activity tests and collagen degradation FT-IR spectroscopic analysis. Pre-treatment with the two CAIs kept the micro tensile values after 12 months of storage (32.23 ± 5.95) and cariogenic challenge (34.13 ± 2.71) similar to the initial, pre-treatment values (33.56 ± 4.34). A decreased Streptococcus mutans biofilm formation on dentine surfaces and antibacterial activity against planktonic bacteria were observed after CAI treatment. Dentine pre-treatment with sulphonamide CAIs maintained adhesion strength stability, allowed better dentine wettability, maintained matrix collagen, and showed anti-S. mutans activity. [ABSTRACT FROM AUTHOR]

Published
2023
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8. Dentine biomodification by sulphonamides pre-treatment: bond strength, proteolytic inhibition, and antimicrobial activity

Author

Portela, Maristela Barbosa, primary, Barboza, Caroliny Mello, additional, da Silva, Eduardo Moreira, additional, de Moraes, Daniel Clemente, additional, Simão, Renata Antoun, additional, de Souza, Clara Ribeiro, additional, Cardoso, Verônica da Silva, additional, Ferreira-Pereira, Antônio, additional, Vermelho, Alane Beatriz, additional, and Supuran, Claudiu T., additional

Published
2022
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