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44 results on '"de Villiers, Etienne P."'

2. Genome Sequence of Theileria parva, a Bovine Pathogen That Transforms Lymphocytes

Author

Gardner, Malcolm J., Bishop, Richard, Shah, Trushar, de Villiers, Etienne P., Carlton, Jane M., Hall, Neil, Ren, Qinghu, Paulsen, Ian T., Pain, Arnab, Berriman, Matthew, Sato, Shigeharu, Ralph, Stuart A., Mann, David J., Xiong, Zikai, Shallom, Shamira J., Weidman, Janice, Jiang, Lingxia, Lynn, Jeffery, Weaver, Bruce, Shoaibi, Azadeh, Domingo, Alexander R., Wasawo, Delia, Crabtree, Jonathan, Wortman, Jennifer R., Haas, Brian, Angiuoli, Samuel V., Creasy, Todd H., Lu, Charles, Suh, Bernard, Silva, Joana C., Utterback, Teresa R., Feldblyum, Tamara V., Pertea, Mihaela, Allen, Jonathan, Nierman, William C., Salzberg, Steven L., White, Owen R., Fitzhugh, Henry A., Morzaria, Subhash, Venter, J. Craig, Fraser, Claire M., and Nene, Vishvanath

Published
2005

3. The Genome of the Heartwater Agent Ehrlichia ruminantium Contains Multiple Tandem Repeats of Actively Variable Copy Number

Author

Collins, Nicola E., Liebenberg, Junita, de Villiers, Etienne P., Brayton, Kelly A., Louw, Elmarié, Pretorius, Alri, Faber, F. Erika, van Heerden, Henriette, Josemans, Antoinette, van Kleef, Mirinda, Steyn, Helena C., van Strijp, M. Fransie, Zweygarth, Erich, Jongejan, Frans, Maillard, Jean Charles, Berthier, David, Botha, Marli, Joubert, Fourie, Corton, Craig H., Thomson, Nicholas R., Allsopp, Maria T., Allsopp, Basil A., and Beaty, Barry J.

Published
2005

5. Repeated clinical malaria episodes are associated with modification of the immune system in children

Author

Bediako, Yaw, Adams, Rhys, Reid, Adam J., Valletta, John Joseph, Ndungu, Francis M., Sodenkamp, Jan, Mwacharo, Jedidah, Ngoi, Joyce Mwongeli, Kimani, Domtila, Kai, Oscar, Wambua, Juliana, Nyangweso, George, de Villiers, Etienne P., Sanders, Mandy, Lotkowska, Magda Ewa, Lin, Jing-Wen, Manni, Sarah, Addy, John W. G., Recker, Mario, Newbold, Chris, Berriman, Matthew, Bejon, Philip, Marsh, Kevin, and Langhorne, Jean

Published
2019
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6. Genome of the Host-Cell Transforming Parasite Theileria annulata Compared with T. parva

Author

Pain, Arnab, Renauld, Hubert, Berriman, Matthew, Murphy, Lee, Yeats, Corin A., Weir, William, Kerhornou, Arnaud, Aslett, Martin, Bishop, Richard, Bouchier, Christiane, Cochet, Madeleine, Cronin, Ann, de Villiers, Etienne P., Fraser, Audrey, Fosker, Nigel, Gardner, Malcolm, Goble, Arlette, Griffiths-Jones, Sam, Harris, David E., Katzer, Frank, Larke, Natasha, Lord, Angela, Maser, Pascal, McKellar, Sue, Mooney, Paul, Morton, Fraser, Nene, Vishvanath, O'Neil, Susan, Price, Claire, Quail, Michael A., Rabbinowitsch, Ester, Rawlings, Neil D., Rutter, Simon, Saunders, David, Seeger, Kathy, Shah, Trushar, Squares, Robert, Squares, Steven, Tivey, Adrian, Walker, Alan R., Woodward, John, Langsley, Gordon, Rajandream, Marie-Adele, McKeever, Declan, Shiels, Brian, Tait, Andrew, Barrell, Bart, and Hall, Neil

Published
2005

7. Theileria

Author

Bishop, Richard P., Odongo, David O., Mann, David J., Pearson, Terry W., Sugimoto, Chihiro, Haines, Lee R., Glass, Elizabeth, Jensen, Kirsty, Seitzer, Ulrike, Ahmed, Jabbar S., Graham, Simon P., de Villiers, Etienne P., Nene, Vishvanath, editor, and Kole, Chittaranjan, editor

Published
2009
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9. Theileria parva Candidate Vaccine Antigens Recognized by Immune Bovine Cytotoxic T Lymphocytes

Author

Graham, Simon P., Pellé, Roger, Honda, Yoshikazu, Mwangi, Duncan M., Tonukari, Nyerhovwo J., Yamage, Mat, Glew, E. Jane, de Villiers, Etienne P., Shah, Trushar, Bishop, Richard, Abuya, Evelyne, Awino, Elias, Gachanja, James, Luyai, Anthony E., Mbwika, Ferdinand, Muthiani, Anthony M., Ndegwa, David M., Njahira, Moses, Nyanjui, John K., Onono, Fredrick O., Osaso, Julius, Saya, Rosemary M., Wildmann, Claude, Fraser, Claire M., Maudlin, Ian, Gardner, Malcolm J., Morzaria, Subhash P., Loosmore, Sheena, Gilbert, Sarah C., Audonnet, Jean-Christophe, van der Bruggen, Pierre, Nene, Vishvanath, and Taracha, Evans L. N.

Published
2006
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25. Micro-epidemiological structuring of Plasmodium falciparum parasite populations in regions with varying transmission intensities in Africa

Author

Omedo, Irene, primary, Mogeni, Polycarp, additional, Bousema, Teun, additional, Rockett, Kirk, additional, Amambua-Ngwa, Alfred, additional, Oyier, Isabella, additional, Stevenson, Jennifer C., additional, Baidjoe, Amrish Y., additional, de Villiers, Etienne P., additional, Fegan, Greg, additional, Ross, Amanda, additional, Hubbart, Christina, additional, Jeffreys, Anne, additional, Williams, Thomas N., additional, Kwiatkowski, Dominic, additional, and Bejon, Philip, additional

Published
2017
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26. Geographic-genetic analysis of Plasmodium falciparum parasite populations from surveys of primary school children in Western Kenya

Author

Omedo, Irene, primary, Mogeni, Polycarp, additional, Rockett, Kirk, additional, Kamau, Alice, additional, Hubbart, Christina, additional, Jeffreys, Anna, additional, Ochola-Oyier, Lynette Isabella, additional, de Villiers, Etienne P., additional, Gitonga, Caroline W., additional, Noor, Abdisalan M., additional, Snow, Robert W., additional, Kwiatkowski, Dominic, additional, and Bejon, Philip, additional

Published
2017
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27. Patterns of nucleotide diversity in Meisa1 and G3pdh in wild and cultivated cassava

Author

Beyene, Dereje, Mukasa, Settumba B., Jansson, Christer, de Villiers, Etienne P., Ferguson, Morag, Rabbi, Ismail Yusuf, Kyamanywa, Samuel, Sebuliba, James, and Baguma, Yona

Subjects
Cassava, G3pdh, Isoamylase1, SNPs, Starch
Abstract

The distribution and frequency of single nucleotide polymorphisms (snps) is an excellent tool for discerning evolutionary relatedness between cultivated and wild plant genomes. This type of information is scanty for the genus Manihot, and thus limiting systematic approaches in the genetic improvement of cassava. Here, we present a detailed description of the comparative patterns of snps in Isoamylase1 (Meisa1) and Glyceraldehyde-3-phosphate dehydrogenase (G3pdh) in 10 accessions of wild (Manihot esculenta subsp. flabellifolia) and 12 accessions of cultivated cassava (M. esculenta). The results show that Meisa1 is more variable in cultivated cassava than that in subspecies flabellifolia, where the 954 bp sequence region differs at 1 in 111 and 250 nucleotides of cultivated and wild species, respectively. Frequency analysis shows that snp occurs once every 42 bp in cultivated and every 70 bp in wild. Tajima’s D test statistics showed that Meisa1 has been evolving under different selection pressures, diversifying in cultivated and purifying in wild. G3pdh is under diversifying selection in both populations. This may indicate the importance for isoamylase1 in starch quality traits in cassava, a trait that is likely to have been the target for artificial selection by farmers and breeders, in addition to natural selection. This study also suggests that G3pdh may be a good marker for phylogeny study while Meisa1 may be useful for intra and inter-cultivar diversity studies. The non-synonymous snps that changed the amino acid property were identified and the potential implication of the change in protein function was analyzed and discussed.

Published
2014

30. TparvaDB: A database to support Theileria parva vaccine development

Author

Visendi, Paul, Ng'ang'a, Wanjiku, Bulimo, Wallace, Bishop, Richard, Ochanda, James, De Villiers, Etienne P., Visendi, Paul, Ng'ang'a, Wanjiku, Bulimo, Wallace, Bishop, Richard, Ochanda, James, and De Villiers, Etienne P.

Abstract

We describe the development of TparvaDB, a comprehensive resource to facilitate research towards development of an East Coast fever vaccine, by providing an integrated user-friendly database of all genome and related data currently available for Theileria parva. TparvaDB is based on the Generic Model Organism Database (GMOD) platform. It contains a complete reference genome sequence, Expressed Sequence Tags (ESTs), Massively Parallel Signature Sequencing (MPSS) expression tag data and related information from both public and private repositories. The Artemis annotation workbench provides online annotation functionality. TparvaDB represents a resource that will underpin and promote ongoing East Coast fever vaccine development and biological research.

Published
2011

31. Characterization of the fine specificity of bovine CD8 T-cell responses to defined antigens from the protozoan parasite Theileria parva.

Author

UCL - SSS/DDUV - Institut de Duve, Graham, Simon P, Pellé, Roger, Yamage, Mat, Mwangi, Duncan M, Honda, Yoshikazu, Mwakubambanya, Ramadhan S, de Villiers, Etienne P, Abuya, Evelyne, Awino, Elias, Gachanja, James, Mbwika, Ferdinand, Muthiani, Anthony M, Muriuki, Cecelia, Nyanjui, John K, Onono, Fredrick O, Osaso, Julius, Riitho, Victor, Saya, Rosemary M, Ellis, Shirley A, McKeever, Declan J, MacHugh, Niall D, Gilbert, Sarah C, Audonnet, Jean-Christophe, Morrison, W Ivan, van der Bruggen, Pierre, Taracha, Evans L N, UCL - SSS/DDUV - Institut de Duve, Graham, Simon P, Pellé, Roger, Yamage, Mat, Mwangi, Duncan M, Honda, Yoshikazu, Mwakubambanya, Ramadhan S, de Villiers, Etienne P, Abuya, Evelyne, Awino, Elias, Gachanja, James, Mbwika, Ferdinand, Muthiani, Anthony M, Muriuki, Cecelia, Nyanjui, John K, Onono, Fredrick O, Osaso, Julius, Riitho, Victor, Saya, Rosemary M, Ellis, Shirley A, McKeever, Declan J, MacHugh, Niall D, Gilbert, Sarah C, Audonnet, Jean-Christophe, Morrison, W Ivan, van der Bruggen, Pierre, and Taracha, Evans L N

Abstract

Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated by CD8 T cells. Six antigens targeted by CD8 T cells from T. parva-immune cattle of different major histocompatibility complex (MHC) genotypes have been identified, raising the prospect of developing a subunit vaccine. To facilitate further dissection of the specificity of protective CD8 T-cell responses and to assist in the assessment of responses to vaccination, we set out to identify the epitopes recognized in these T. parva antigens and their MHC restriction elements. Nine epitopes in six T. parva antigens, together with their respective MHC restriction elements, were successfully identified. Five of the cytotoxic-T-lymphocyte epitopes were found to be restricted by products of previously described alleles, and four were restricted by four novel restriction elements. Analyses of CD8 T-cell responses to five of the epitopes in groups of cattle carrying the defined restriction elements and immunized with live parasites demonstrated that, with one exception, the epitopes were consistently recognized by animals of the respective genotypes. The analysis of responses was extended to animals immunized with multiple antigens delivered in separate vaccine constructs. Specific CD8 T-cell responses were detected in 19 of 24 immunized cattle. All responder cattle mounted responses specific for antigens for which they carried an identified restriction element. By contrast, only 8 of 19 responder cattle displayed a response to antigens for which they did not carry an identified restriction element. These data demonstrate that the identified antigens are inherently dominant in animals with the corresponding MHC genotypes.

Published
2008

32. Camel Streptococcus agalactiae populations are associated with specific disease complexes and acquired the tetracycline resistance gene tetM via a Tn916-like element

Author

Fischer, Anne, primary, Liljander, Anne, additional, Kaspar, Heike, additional, Muriuki, Cecilia, additional, Fuxelius, Hans-Henrik, additional, Bongcam-Rudloff, Erik, additional, de Villiers, Etienne P, additional, Huber, Charlotte A, additional, Frey, Joachim, additional, Daubenberger, Claudia, additional, Bishop, Richard, additional, Younan, Mario, additional, and Jores, Joerg, additional

Published
2013
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34. A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology.

Author

UCL - SSS/DDUV - Institut de Duve, Graham, Simon P, Honda, Yoshikazu, Pellé, Roger, Mwangi, Duncan M, Glew, E Jane, de Villiers, Etienne P, Shah, Trushar, Bishop, Richard, van der Bruggen, Pierre, Nene, Vishvanath, Taracha, Evans L N, UCL - SSS/DDUV - Institut de Duve, Graham, Simon P, Honda, Yoshikazu, Pellé, Roger, Mwangi, Duncan M, Glew, E Jane, de Villiers, Etienne P, Shah, Trushar, Bishop, Richard, van der Bruggen, Pierre, Nene, Vishvanath, and Taracha, Evans L N

Abstract

Immunity against the bovine protozoan parasite Theileria parva has previously been shown to be mediated through lysis of parasite-infected cells by MHC class I restricted CD8+ cytotoxic T lymphocytes. It is hypothesized that identification of CTL target schizont antigens will aid the development of a sub-unit vaccine. We exploited the availability of the complete genome sequence data and bioinformatics tools to identify genes encoding secreted or membrane anchored proteins that may be processed and presented by the MHC class I molecules of infected cells to CTL.

Published
2007

36. Ten Simple Rules for Organizing a Virtual Conference—Anywhere

Author

Gichora, Nelson N., primary, Fatumo, Segun A., additional, Ngara, Mtakai V., additional, Chelbat, Noura, additional, Ramdayal, Kavisha, additional, Opap, Kenneth B., additional, Siwo, Geoffrey H., additional, Adebiyi, Marion O., additional, El Gonnouni, Amina, additional, Zofou, Denis, additional, Maurady, Amal A. M., additional, Adebiyi, Ezekiel F., additional, de Villiers, Etienne P., additional, Masiga, Daniel K., additional, Bizzaro, Jeffrey W., additional, Suravajhala, Prashanth, additional, Ommeh, Sheila C., additional, and Hide, Winston, additional

Published
2010
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38. Characterization of the Fine Specificity of Bovine CD8 T-Cell Responses to Defined Antigens from the Protozoan ParasiteTheileria parva

Author

Graham, Simon P., primary, Pellé, Roger, additional, Yamage, Mat, additional, Mwangi, Duncan M., additional, Honda, Yoshikazu, additional, Mwakubambanya, Ramadhan S., additional, de Villiers, Etienne P., additional, Abuya, Evelyne, additional, Awino, Elias, additional, Gachanja, James, additional, Mbwika, Ferdinand, additional, Muthiani, Anthony M., additional, Muriuki, Cecelia, additional, Nyanjui, John K., additional, Onono, Fredrick O., additional, Osaso, Julius, additional, Riitho, Victor, additional, Saya, Rosemary M., additional, Ellis, Shirley A., additional, McKeever, Declan J., additional, MacHugh, Niall D., additional, Gilbert, Sarah C., additional, Audonnet, Jean-Christophe, additional, Morrison, W. Ivan, additional, van der Bruggen, Pierre, additional, and Taracha, Evans L. N., additional

Published
2008
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40. Characterization of the Fine Specificity of Bovine CD8 T-Cell Responses to Defined Antigens from the Protozoan Parasite Theileria parva

Author

Graham, Simon P., Pellé, Roger, Yamage, Mat, Mwangi, Duncan M., Honda, Yoshikazu, Mwakubambanya, Ramadhan S., de Villiers, Etienne P., Abuya, Evelyne, Awino, Elias, Gachanja, James, Mbwika, Ferdinand, Muthiani, Anthony M., Muriuki, Cecelia, Nyanjui, John K., Onono, Fredrick O., Osaso, Julius, Riitho, Victor, Saya, Rosemary M., Ellis, Shirley A., McKeever, Declan J., MacHugh, Niall D., Gilbert, Sarah C., Audonnet, Jean-Christophe, Morrison, W. Ivan, van der Bruggen, Pierre, and Taracha, Evans L. N.

Abstract

ABSTRACTImmunity against the bovine intracellular protozoan parasite Theileria parvahas been shown to be mediated by CD8 T cells. Six antigens targeted by CD8 T cells from T. parva-immune cattle of different major histocompatibility complex (MHC) genotypes have been identified, raising the prospect of developing a subunit vaccine. To facilitate further dissection of the specificity of protective CD8 T-cell responses and to assist in the assessment of responses to vaccination, we set out to identify the epitopes recognized in these T. parvaantigens and their MHC restriction elements. Nine epitopes in six T. parvaantigens, together with their respective MHC restriction elements, were successfully identified. Five of the cytotoxic-T-lymphocyte epitopes were found to be restricted by products of previously described alleles, and four were restricted by four novel restriction elements. Analyses of CD8 T-cell responses to five of the epitopes in groups of cattle carrying the defined restriction elements and immunized with live parasites demonstrated that, with one exception, the epitopes were consistently recognized by animals of the respective genotypes. The analysis of responses was extended to animals immunized with multiple antigens delivered in separate vaccine constructs. Specific CD8 T-cell responses were detected in 19 of 24 immunized cattle. All responder cattle mounted responses specific for antigens for which they carried an identified restriction element. By contrast, only 8 of 19 responder cattle displayed a response to antigens for which they did not carry an identified restriction element. These data demonstrate that the identified antigens are inherently dominant in animals with the corresponding MHC genotypes.

Published
2008
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41. Who will bear moral responsibility?

Author

de Villiers, Etienne

Abstract

One of the most disturbing paradoxes of modern times is that an acute sense of expanding moral responsibility goes hand in hand with a growing inability to bear such responsibility. This article demonstrates how modern technology, in particular information technology, has contributed to both the expansion of moral responsibility and the development of the responsibility gap. In order to deal adequately with the challenges modern technology poses in respect of moral responsibility, it has become necessary to supplement the classical notion of retrospective moral responsibility with the new notion of prospective moral responsibility. In the case of retrospective responsibility some or other negative outcome of the past is ascribed to a particular person or persons. In the case of prospective responsibility, the responsibility of preventing humans and nature from being actively harmed or to realise desirable future conditions by taking the necessary measures in the present, is ascribed to a particular person or persons, or, more likely, to a particular institution or institutions.

Published
2002
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42. THE CHANGING MAN.

Author

De Villiers, Etienne

Abstract

This article features Etienne de Villiers, president of the Association of Tennis Professionals (ATP) Tour. He believes that revamping the calendar and rebranding the competitions will benefit the players, create more value for the tournaments themselves, and make it easier for fans to relate to the sport. A detailed description of the changes to the ATP Tour is presented, as well as biographical information on de Villiers.

Published
2008

43. TparvaDB: a database to support Theileria parva vaccine development.

Author

Visendi P, Ng'ang'a W, Bulimo W, Bishop R, Ochanda J, and de Villiers EP

Subjects
Expressed Sequence Tags, Internet, Molecular Sequence Annotation, User-Computer Interface, Databases, Genetic, Protozoan Vaccines immunology, Theileria parva genetics, Theileria parva immunology
Abstract

We describe the development of TparvaDB, a comprehensive resource to facilitate research towards development of an East Coast fever vaccine, by providing an integrated user-friendly database of all genome and related data currently available for Theileria parva. TparvaDB is based on the Generic Model Organism Database (GMOD) platform. It contains a complete reference genome sequence, Expressed Sequence Tags (ESTs), Massively Parallel Signature Sequencing (MPSS) expression tag data and related information from both public and private repositories. The Artemis annotation workbench provides online annotation functionality. TparvaDB represents a resource that will underpin and promote ongoing East Coast fever vaccine development and biological research. Database URL: http://tparvadb.ilri.cgiar.org.

Published
2011
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44. A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology.

Author

Graham SP, Honda Y, Pellé R, Mwangi DM, Glew EJ, de Villiers EP, Shah T, Bishop R, van der Bruggen P, Nene V, and Taracha EL

Abstract

Background: Immunity against the bovine protozoan parasite Theileria parva has previously been shown to be mediated through lysis of parasite-infected cells by MHC class I restricted CD8+ cytotoxic T lymphocytes. It is hypothesized that identification of CTL target schizont antigens will aid the development of a sub-unit vaccine. We exploited the availability of the complete genome sequence data and bioinformatics tools to identify genes encoding secreted or membrane anchored proteins that may be processed and presented by the MHC class I molecules of infected cells to CTL., Results: Of the 986 predicted open reading frames (ORFs) encoded by chromosome 1 of the T. parva genome, 55 were selected based on the presence of a signal peptide and/or a transmembrane helix domain. Thirty six selected ORFs were successfully cloned into a eukaryotic expression vector, transiently transfected into immortalized bovine skin fibroblasts and screened in vitro using T. parva-specific CTL. Recognition of gene products by CTL was assessed using an IFN-gamma ELISpot assay. A 525 base pair ORF encoding a 174 amino acid protein, designated Tp2, was identified by T. parva-specific CTL from 4 animals. These CTL recognized and lysed Tp2 transfected skin fibroblasts and recognized 4 distinct epitopes. Significantly, Tp2 specific CD8+ T cell responses were observed during the protective immune response against sporozoite challenge., Conclusion: The identification of an antigen containing multiple CTL epitopes and its apparent immunodominance during a protective anti-parasite response makes Tp2 an attractive candidate for evaluation of its vaccine potential.

Published
2007
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