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12 results on '"de los Angeles García, María"'

6. Evaluation of specific humoral immune response and cross reactivity against Mycobacterium tuberculosis antigens induced in mice immunized with liposomes composed of total lipids extracted from Mycobacterium smegmatis

Author

de los Angeles García, María, primary, Borrero, Reinier, additional, Marrón, Reynel, additional, Lanio, María E, additional, Canet, Lien, additional, Otero, Oscar, additional, Kadir, Ramlah, additional, Suraiya, Siti, additional, Zayas, Caridad, additional, López, Yamilé, additional, Nor Norazmi, Mohd, additional, Sarmiento, Maria E, additional, and Acosta, Armando, additional

Published
2013
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7. Differential distribution of the Sodium-vitamin C cotransporter-1 along the proximal tubule of the mouse and human kidney

Author

Castro, Tamara, primary, Low, Marcela, additional, Salazar, Katterine, additional, Montecinos, Hernán, additional, Cifuentes, Manuel, additional, Yáñez, Alejandro J., additional, Slebe, Juan Carlos, additional, Figueroa, Carlos D., additional, Reinicke, Karin, additional, de los Angeles García, María, additional, Henriquez, Juan Pablo, additional, and Nualart, Francisco, additional

Published
2008
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10. Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol

Author

Klattenhoff, Carla, primary, Montecino, Martín, additional, Soto, Ximena, additional, Guzmán, Leonardo, additional, Romo, Ximena, additional, de los Angeles García, María, additional, Mellstrom, Britt, additional, Naranjo, José Ramón, additional, Hinrichs, María Victoria, additional, and Olate, Juan, additional

Published
2003
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11. Expresión heteróloga de un péptido multiepitópico de células B de M. tuberculosis en Saccharomyces cerevisiae.

Author

De los Angeles García, María, Sarmiento, María Elena, Coria, Roberto, Kawasaky, Laura, Ongay, Laura, De la Rosa, Juan Francisco, and Acosta, Armando

Subjects
*SACCHAROMYCES cerevisiae, *TUBERCULOSIS, *VACCINATION, *PROTEINS, *WESTERN immunoblotting
Abstract

Saccharomyces cerevisiae has been widely used as expression system of heterologous proteins. The aim of the present work was the expression in Saccharomyces cerevisiae of a class B multiepitopic peptide from M. tuberculosis. For this purpose, the chimerical peptide named B2 was cloned in two yeast expression vectors containing galactose and cupric sulphate regulated promoters, respectively. After induction experiments, B2 expression was analyzed by SDS/PAGE and Western Blot. By Western Blot analysis B2 expression was confirmed when induction took place overnight with 100 µM of CuSO4. No expression signal took place when galactose was used as inductor. These results show that Sacchromyces cervisiae could be a good alternative host for the expression of multiepitopic peptides from M. tuberculosis. [ABSTRACT FROM AUTHOR]

Published
2007

12. Cytogenetic damage in female Chilean agricultural workers exposed to mixtures of pesticides

Author

Márquez, Carolina, Villalobos, Cecilia, Poblete, Susana, Villalobos, Eva, de los Angeles García, María, and Duk, Soledad

Abstract

The VIII Region of Bío‐Bío is a major fruit‐growing area of Chile that makes intensive use of agricultural pesticides. The cytogenetic damage associated with exposure to mixtures of pesticides was evaluated by comparing peripheral blood lymphocyte micronucleus (MN) frequencies in a group of 64 female agricultural workers and 30 female controls. The exposed subjects worked during the spring and summer in thinning and pruning fruit trees and in harvesting and packing different fruits, such as raspberries, grapes, apples, and kiwis. They did not use any protective measures during their work activities. A significant increase in the frequency of binucleated cells with micronuclei (BNMN) was found in the exposed women as compared with the controls (36.94 ± 14.47 vs. 9.93 ± 6.17 BNMN/1000 BN cells; P< 0.001). The frequency of BNMN varied as a function of age in both the exposed and control groups, but no correlation was found between BNMN frequency and the duration of exposure. Also, smoking and other habits had no effect on MN frequency. Our study confirms that occupational exposure to pesticide mixtures results in cytogenetic damage. Environ. Mol. Mutagen., 2005. © 2004 Wiley‐Liss, Inc.

Published
2005
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