1. Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology.
- Author
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Groenen, Patricia J. T. A., den Brand, Michiel van, Kroeze, Leonie I., Amir, Avital L., and Hebeda, Konnie M.
- Subjects
GENE rearrangement ,LYMPHOCYTES ,PRIMARY immunodeficiency diseases ,T-cell lymphoma ,PATHOLOGY ,AUTOIMMUNE diseases - Abstract
Clonality assessment using the unique rearrangements of immunoglobulin (IG) and T-cell receptor (TR) genes in lymphocytes is a widely applied supplementary test for the diagnosis of B-cell and T-cell lymphoma. To enable a more sensitive detection and a more precise comparison of clones compared with conventional clonality analysis based on fragment analysis, the EuroClonality NGS Working Group developed and validated a next-generation sequencing (NGS)-based clonality assay for detection of the IG heavy and kappa light chain and TR gene rearrangements for formalin-fixed and paraffin-embedded tissues. We outline the features and advantages of NGS-based clonality detection and discuss potential applications for NGS-based clonality testing in pathology, including site specific lymphoproliferations, immunodeficiency and autoimmune disease and primary and relapsed lymphomas. Also, we briefly discuss the role of T-cell repertoire of reactive lymphocytic infiltrations in solid tumors and B-lymphoma. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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