43 results on '"di Gioia, Cira R. T."'
Search Results
2. Sodium Glucose Cotransporter-2 Inhibitors in Non-Diabetic Kidney Disease: Evidence in Experimental Models.
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Castoldi, Giovanna, Carletti, Raffaella, Barzaghi, Francesca, Meani, Michela, Zatti, Giovanni, Perseghin, Gianluca, Di Gioia, Cira R. T., and Zerbini, Gianpaolo
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DIABETIC nephropathies ,KIDNEY diseases ,TYPE 2 diabetes ,SODIUM-glucose cotransporter 2 inhibitors ,GLYCEMIC control ,GLUCOSE ,GLYCOSYLATED hemoglobin - Abstract
Sodium glucose cotransporter 2 (SGLT2) inhibitors are a class of glucose-lowering agents widely used for the treatment of type 2 diabetes mellitus. A number of clinical trials in type 2 diabetic patients with different degrees of renal impairment have clearly demonstrated that SGLT2 inhibitors reduce the progression rate of diabetic kidney disease. Furthermore, recent studies have shown that SGLT2 inhibitors also exert a protective effect in the case of non-diabetic kidney disease. Consequently, it has been hypothesized that the nephroprotective activity of these drugs could exceed the canonical impact on glycemic control and that the resulting beneficial effects could be the consequence of their pleiotropic properties (proven reduction of inflammation, fibrosis, oxidative stress and sympathetic nervous activity) both at systemic and tissue levels, suggesting that the efficacy of these drugs could also be extended to non-diabetic nephropathies. This review focuses on the nephroprotective effects of SGLT2 inhibitors in different experimental models of non-diabetic kidney disease. The different glucose-independent mechanisms potentially implemented by SGLT2 inhibitors to ultimately protect the non-diabetic kidney are described in detail, and conflicting results, when present, are discussed. [ABSTRACT FROM AUTHOR] more...
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- 2024
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Catalog
3. Cardioprotective Effects of Sodium Glucose Cotransporter 2 Inhibition in Angiotensin II-Dependent Hypertension Are Mediated by the Local Reduction of Sympathetic Activity and Inflammation
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Castoldi, Giovanna, primary, Carletti, Raffaella, additional, Ippolito, Silvia, additional, Colzani, Massimiliano, additional, Pelucchi, Sara, additional, Zerbini, Gianpaolo, additional, Perseghin, Gianluca, additional, Zatti, Giovanni, additional, and di Gioia, Cira R. T., additional more...
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- 2023
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4. Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone
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Castoldi, Giovanna, primary, Carletti, Raffaella, additional, Ippolito, Silvia, additional, Villa, Isabella, additional, Palmisano, Biagio, additional, Bolamperti, Simona, additional, Rubinacci, Alessandro, additional, Zerbini, Gianpaolo, additional, Meani, Michela, additional, Zatti, Giovanni, additional, and di Gioia, Cira R. T., additional more...
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- 2022
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5. Angiotensin II Modulates Calcium/Phosphate Excretion in Experimental Model of Hypertension: Focus on Bone
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Castoldi, G, Carletti, R, Ippolito, S, Villa, I, Palmisano, B, Bolamperti, S, Rubinacci, A, Zerbini, G, Meani, M, Zatti, G, di Gioia, C, Castoldi, Giovanna, Carletti, Raffaella, Ippolito, Silvia, Villa, Isabella, Palmisano, Biagio, Bolamperti, Simona, Rubinacci, Alessandro, Zerbini, Gianpaolo, Meani, Michela, Zatti, Giovanni, di Gioia, Cira R. T., Castoldi, G, Carletti, R, Ippolito, S, Villa, I, Palmisano, B, Bolamperti, S, Rubinacci, A, Zerbini, G, Meani, M, Zatti, G, di Gioia, C, Castoldi, Giovanna, Carletti, Raffaella, Ippolito, Silvia, Villa, Isabella, Palmisano, Biagio, Bolamperti, Simona, Rubinacci, Alessandro, Zerbini, Gianpaolo, Meani, Michela, Zatti, Giovanni, and di Gioia, Cira R. T. more...
- Abstract
A link between hypertension and long-term bone health has been suggested. The aim of this study was to investigate the effects of chronic angiotensin II administration on urinary calcium/phosphate excretion, bone mineral density, bone remodeling and osteoblast population in a well-established experimental model of hypertension, in the absence of possible confounding factors that could affect bone metabolism. Male Sprague–Dawley rats, divided in the following groups: (a) Angiotensin II (Ang II, 200 ng/kg/min, osmotic minipumps, sub cutis, n = 8); (b) Ang II+losartan (Los, 50 mg/kg/day, per os, n = 6); (c) control group (physiological saline, sub cutis, n = 9); and (d) control+losartan (n = 6) were treated for four weeks. During the experimental period, 24-hour diuresis, urinary calcium, phosphate and sodium excretion were measured prior to the treatment, at two weeks of treatment, and at the end of the treatment. Systolic blood pressure was measured by plethysmography technique (tail cuff method). At the end of the experimental protocol, the rats were euthanized and peripheral quantitative computed tomography at the proximal metaphysis and at the diaphysis of the tibiae and quantitative bone histomorphometry on distal femora were performed. Angiotensin II-dependent hypertension is associated with increased calcium and phosphate excretion. AT1 receptor blockade prevented the increase of blood pressure and phosphate excretion but did not affect the increase of calcium excretion. These changes took place without significantly affecting bone density, bone histology or osteoblast population. In conclusion, in our experimental conditions, angiotensin II-dependent hypertension gave rise to an increased urinary excretion of calcium and phosphate without affecting bone density. more...
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- 2022
6. Evaluation of Browning Markers in Subcutaneous Adipose Tissue of Newly Diagnosed Gastrointestinal Cancer Patients with and without Cachexia
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Molfino, Alessio, primary, Belli, Roberta, additional, Imbimbo, Giovanni, additional, Carletti, Raffaella, additional, Amabile, Maria Ida, additional, Tambaro, Federica, additional, di Gioia, Cira R. T., additional, Belloni, Elena, additional, Ferraro, Elisabetta, additional, Nigri, Giuseppe, additional, and Muscaritoli, Maurizio, additional more...
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- 2022
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7. Angiotensin Type 2 and Mas Receptor Activation Prevents Myocardial Fibrosis and Hypertrophy through the Reduction of Inflammatory Cell Infiltration and Local Sympathetic Activity in Angiotensin II-Dependent Hypertension
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Castoldi, Giovanna, primary, Carletti, Raffaella, additional, Ippolito, Silvia, additional, Stella, Andrea, additional, Zerbini, Gianpaolo, additional, Pelucchi, Sara, additional, Zatti, Giovanni, additional, and di Gioia, Cira R. T., additional more...
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- 2021
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8. Sodium-glucose cotransporter 2 inhibition prevents renal fibrosis in cyclosporine nephropathy
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Castoldi, Giovanna, primary, Carletti, Raffaella, additional, Ippolito, Silvia, additional, Colzani, Massimiliano, additional, Barzaghi, Francesca, additional, Stella, Andrea, additional, Zerbini, Gianpaolo, additional, Perseghin, Gianluca, additional, Zatti, Giovanni, additional, and di Gioia, Cira R. T., additional more...
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- 2021
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9. In vivo clinical and histological thermal effect of a 445 nm diode laser on oral soft tissues during a biopsy
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Palaia, Gaspare, primary, D’Alessandro, Leonardo, additional, Pergolini, Daniele, additional, Carletti, Raffaella, additional, Di Gioia, Cira R. T., additional, and Romeo, Umberto, additional
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- 2021
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10. Asymptomatic right atrial myxoma in acromegalic man: a case of Carney complex
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IACOBELLIS, GIANLUCA, DI GIOIA, CIRA R T, and TAMBURRANO, GUIDO
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- 2001
11. Angiolymphoid Hyperplasia with Eosinophilia and Radial Artery Pseudoaneurysm: A Case Report
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Petrakis, Ioannis E, Di Gioia, Cira R. T, and Sciacca, Vincenzo
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- 2000
12. Activation of angiotensin type 2 (AT2) receptors prevents myocardial hypertrophy in Zucker diabetic fatty rats
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Castoldi, G, di Gioia, C, Roma, F, Carletti, R, Manzoni, G, Stella, A, Zerbini, G, Perseghin, G, Castoldi, Giovanna, di Gioia, Cira R. T., Roma, Francesca, Carletti, Raffaella, Manzoni, Giuseppina, Stella, Andrea, Zerbini, Gianpaolo, Perseghin, Gianluca, Castoldi, G, di Gioia, C, Roma, F, Carletti, R, Manzoni, G, Stella, A, Zerbini, G, Perseghin, G, Castoldi, Giovanna, di Gioia, Cira R. T., Roma, Francesca, Carletti, Raffaella, Manzoni, Giuseppina, Stella, Andrea, Zerbini, Gianpaolo, and Perseghin, Gianluca more...
- Abstract
Aims: Compound 21 (C21), selective AT2 receptor agonist, has cardioprotective effects in experimental models of hypertension and myocardial infarction. The aims of the study was to evaluate the effect of C21, losartan, or both in Zucker diabetic fatty (ZDF) rats (type 2 diabetes) on (1) the prevention of myocardial hypertrophy; (2) myocardial expression of phosphatase and tensin homolog (PTEN), a target gene of miR-30a-3p, involved in myocardial remodelling. Methods: Experiments were performed in ZDF (n = 33) and in control Lean (8) rats. From the 6th to the 20th week of age, we administered C21 (0.3 mg/kg/day) to 8 ZDF rats. 8 ZDF rats were treated with losartan (10 mg/kg/day), 8 rats underwent combination treatment, C21+ losartan, and 9 ZDF rats were left untreated. Blood glucose and blood pressure were measured every 4 weeks. At the end of the study the hearts were removed, the apex was cut for the quantification of PTEN mRNA and miR-30a-3p expression (realtime-PCR). Myocardial hypertrophy was evaluated by histomorphometric analysis, and nitrotyrosine expression (as marker of oxidative stress) by immunohistochemistry. Results: ZDF rats had higher blood glucose (p < 0.0001) with respect to control Lean rats, while blood pressure did not change. Both parameters were not modified by C21 treatment, while losartan and losartan + C21 reduced blood pressure in ZDF rats (p < 0.05). miR-30a-3p expression was increased in ZDF rats (p < 0.01) and PTEN mRNA expression was decreased (p < 0.05). ZDF rats developed myocardial hypertrophy (p < 0.01) and increased oxidative stress (p < 0.01), both were prevented by C21 or losartan, or combination treatment. C21 or losartan normalized the expression of miR-30a-3p and PTEN. Conclusions: Activation of AT2 receptors or AT1 receptor blockade prevents the development of myocardial hypertrophy in ZDF rats. This occurs through the modulation of the miR-30a-3p/PTEN interaction. more...
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- 2019
13. Activation of angiotensin type 2 (AT2) receptors prevents myocardial hypertrophy in Zucker diabetic fatty rats
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Castoldi, Giovanna, primary, di Gioia, Cira R. T., additional, Roma, Francesca, additional, Carletti, Raffaella, additional, Manzoni, Giuseppina, additional, Stella, Andrea, additional, Zerbini, Gianpaolo, additional, and Perseghin, Gianluca, additional more...
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- 2018
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14. Renal Anti-Fibrotic Effect of Sodium Glucose Cotransporter 2 Inhibition in Angiotensin II-Dependent Hypertension.
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Castoldi, Giovanna, Carletti, Raffaella, Ippolito, Silvia, Colzani, Massimiliano, Barzaghi, Francesca, Stella, Andrea, Zerbini, Gianpaolo, Perseghin, Gianluca, di Gioia, Cira R.T., and di Gioia, Cira R T more...
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SODIUM-glucose cotransporters ,EMPAGLIFLOZIN ,SPRAGUE Dawley rats ,RENAL fibrosis ,ANGIOTENSINS ,CATABOLITE repression ,BLOOD pressure ,CD14 antigen ,KIDNEY disease prevention ,BENZENE ,HYPERTENSION ,RESEARCH ,KIDNEYS ,ANIMAL experimentation ,RESEARCH methodology ,GLYCOSIDES ,FIBROSIS ,EVALUATION research ,MEDICAL cooperation ,RATS ,KIDNEY diseases ,COMPARATIVE studies ,ANGIOTENSIN II ,DISEASE complications - Abstract
Background: Clinical trials have shown that empagliflozin (Empa), a sodium-glucose cotransporter 2 (SGLT2) inhibitor, promotes nephroprotective effects in diabetic patients. The mechanisms underlying nephroprotection are not completely known and it is not known whether the renal beneficial action is present even in non-diabetic kidney disease. The aim of this study was to evaluate the effect of Empa administration on the development of renal fibrosis in an experimental model of angiotensin II (Ang II)-dependent hypertension.Methods: Sprague Dawley rats (n = 31) were divided into 4 experimental groups. Ang II (200 ng/kg/min, osmotic minipumps, s.c., n = 9) or Ang II + Empa (10 mg/kg/day, per os, n = 10) were administered for 2 weeks. Control rats were treated with placebo (physiological saline, n = 6), and another group was treated with placebo plus Empa (n = 6) for the same period. Blood pressure (plethysmographic method) was measured at the beginning and at the end of the experimental protocol. After 2 weeks, the rats were euthanized and the kidneys were excised for histomorphometric evaluation of glomerular and tubulo-interstitial fibrosis and for the immunohistochemical evaluation of inflammatory infiltrates (monocytes/macrophages) and types I and IV collagen expression.Results: The administration of Ang II resulted in an increase in blood pressure (p < 0.01), glomerular (p < 0.05) and tubulo-interstitial (p < 0.01) fibrosis, renal inflammatory infiltrates (p < 0.01) and type I (p < 0.01) and type IV collagen expression (p < 0.05) compared to the control group. Treatment with Empa did not significantly modify the increase in blood pressure due to Ang II, but prevented the development of renal glomerular and tubulo-interstitial fibrosis, and the increase in inflammatory infiltrates and types I and IV collagen expression in Ang II-treated rats (p < 0.01).Conclusions: These data demonstrate that the treatment with Empa prevents the development of renal fibrosis in Ang II-dependent hypertension. In Ang II-dependent hypertension, the anti-fibrotic effect due to SGLT2 inhibition is caused by the reduction of inflammatory infiltrates and it is independent on the modulation of blood pressure increase. [ABSTRACT FROM AUTHOR] more...- Published
- 2020
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15. Correlating the morphological features of tetralogy of Fallot and the Eisenmenger malformation
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Restivo, Angelo, primary, Anderson, Robert H., additional, Carletti, Raffaella, additional, and di Gioia, Cira R. T., additional
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- 2016
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16. The Eisenmenger malformation: a morphologic study
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Restivo, Angelo, primary, di Gioia, Cira R. T., additional, Anderson, Robert H., additional, Carletti, Raffaella, additional, and Gallo, Pietro, additional
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- 2015
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17. Prevention of diabetic nephropathy by compound 21, selective agonist of angiotensin type 2 receptors, in Zucker diabetic fatty rats
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Castoldi, Giovanna, primary, di Gioia, Cira R. T., additional, Bombardi, Camila, additional, Maestroni, Silvia, additional, Carletti, Raffaella, additional, Steckelings, U. Muscha, additional, Dahlöf, Bjorn, additional, Unger, Thomas, additional, Zerbini, Gianpaolo, additional, and Stella, Andrea, additional more...
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- 2014
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18. Correlating the morphological features of tetralogy of Fallot and the Eisenmenger malformation.
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Restivo, Angelo, Anderson, Robert H., Carletti, Raffaella, and di Gioia, Cira R. T.
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- 2017
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19. Angiotensin type-2 (AT-2)-receptor activation reduces renal fibrosis in cyclosporine nephropathy: evidence for blood pressure independent effect.
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Castoldi, Giovanna, di Gioia, Cira R. T., Carletti, Raffaella, Roma, Francesca, Zerbini, Gianpaolo, and Stella, Andrea
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ANGIOTENSINS , *HYPERTENSION , *CYCLOSPORINE , *SPRAGUE Dawley rats , *DIABETES - Abstract
Compound 21 (C21), selective agonist of angiotensin type-2 (AT-2) receptors, shows anti-inflammatory effects in experimental models of hypertension and nephroprotection in diabetes. The aim of the present study was to evaluate the effects of C21 in cyclosporine nephropathy, which is characterized mainly by tubulo-interstitial fibrosis. Ten days before and during the experimental periods, low-salt diet was administered to Sprague-Dawley rats. Cyclosporine-A (CsA; 15 mg/kg per day, intraperitoneal injection) and CsA plus C21 (0.3 mg/kg per day, intraperitoneal injection) were administered for 1 and 4 weeks. Control groups were left without any treatment. Blood pressure (plethysmographic method) and 24 h urinary albumin excretion were measured once a week. At the end of the experimental protocols, the kidneys were excised for histomorphometric analysis of renal fibrosis and for immunohistochemical evaluation of inflammatory infiltrates and type I and type IV collagen expression. After 1 and 4 weeks, the rats treated with CsA showed a significant increase (P<0.01) in blood pressure, no significant changes in urinary albumin excretion and a significant increase (P<0.01) in glomerular and tubulo-interstitial fibrosis and inflammatory infiltrates as compared with the control rats. Treatment with C21 did not modify the CsA dependent increase of blood pressure, which was higher than in control rats, but after 4 weeks of treatment significantly reduced (P<0.01) glomerular and tubulo-interstitial fibrosis, type 1 collagen expression and macrophage infiltration, as compared with rats treated with cyclosporine. The administration of C21 showed a protective effect on cyclosporine nephropathy, decreasing renal fibrosis and macrophage infiltration. These data suggest that C21 may counteract tubulo-interstitial fibrosis, the most potent predictor of the progression of renal diseases. [ABSTRACT FROM AUTHOR] more...
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- 2016
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20. Anatomical Lymph Node Mapping in Normal Mesorectal Adipose Tissue
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Miscusi, Giandomenico, primary, di Gioia, Cira R. T., additional, Patrizi, Gregorio, additional, Gravetz, Aviad, additional, Redler, Adriano, additional, and Petrozza, Vincenzo, additional
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- 2010
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21. Prevention of myocardial fibrosis by N-acetyl-seryl-aspartyl-lysyl-proline in diabetic rats
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Castoldi, Giovanna, primary, Di Gioia, Cira R. T., additional, Bombardi, Camila, additional, Perego, Carla, additional, Perego, Lucia, additional, Mancini, Massimiliano, additional, Leopizzi, Martina, additional, Corradi, Barbara, additional, Perlini, Stefano, additional, Zerbini, Gianpaolo, additional, and Stella, Andrea, additional more...
- Published
- 2010
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22. The Eisenmenger malformation: a morphologic study.
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Restivo, Angelo, di Gioia, Cira R. T., Anderson, Robert H., Carletti, Raffaella, and Gallo, Pietro
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- 2016
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23. Angiotensin II modulates frizzled-2 receptor expression in rat vascular smooth muscle cells
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CASTOLDI, Giovanna, primary, REDAELLI, Serena, additional, van de GREEF, Willy M. M., additional, di GIOIA, Cira R. T., additional, BUSCA, Giuseppe, additional, SPERTI, Giovanni, additional, and STELLA, Andrea, additional more...
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- 2005
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24. Neonatal Williams Syndrome Presenting as an Isolated Supravalvular Pulmonary Stenosis
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di Gioia, Cira R. T., primary, Ciallella, Costantino, additional, d'Amati, Giulia, additional, Parroni, Eleonora, additional, Nardone, Anna Maria, additional, and Gallo, Pietro, additional
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- 2003
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25. ANG II increases TIMP-1 expression in rat aortic smooth muscle cells in vivo
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Castoldi, Giovanna, primary, di Gioia, Cira R. T., additional, Pieruzzi, Federico, additional, D'Orlando, Cristina, additional, van de Greef, Willy M. M., additional, Busca, Giuseppe, additional, Sperti, Giovanni, additional, and Stella, Andrea, additional more...
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- 2003
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26. Angiotensin II modulates calponin gene expression in rat vascular smooth muscle cells in vivo
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Castoldi, Giovanna, primary, di Gioia, Cira R. T., additional, Pieruzzi, Federico, additional, van de Greef, Willy M. M., additional, Busca, Giuseppe, additional, Sperti, Giovanni, additional, and Stella, Andrea, additional more...
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- 2001
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27. Is Apoptosis a Diagnostic Marker of Acute Myocardial Infarction?
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Piro, Francesca R., primary, di Gioia, Cira R. T., additional, Gallo, Pietro, additional, Giordano, Carla, additional, and d'Amati, Giulia, additional
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- 2000
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28. Myocyte Transdifferentiation
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d'Amati, Giulia, primary, di Gioia, Cira R. T., additional, Giordano, Carla, additional, and Gallo, Pietro, additional
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- 2000
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29. Prevention of diabetic nephropathy by compound 21, selective agonist of angiotensin type 2 receptors, in Zucker diabetic fatty rats.
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Castoldi, Giovanna, di Gioia, Cira R. T., Bombardi, Camila, Maestroni, Silvia, Carletti, Raffaella, Steckelings, U. Muscha, Dahlöf, Bjorn, Unger, Thomas, Zerbini, Gianpaolo, and Stella, Andrea
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DIABETIC nephropathies , *ANGIOTENSIN receptors , *LABORATORY rats , *ALBUMINURIA , *RENAL fibrosis , *LOSARTAN , *PREVENTION , *THERAPEUTICS - Abstract
The aim of this study was to evaluate the effect of compound 21 (C21), a selective AT2 receptor agonist, on diabetic nephropathy and the potential additive effect of C21, when associated with losartan treatment, on the development of albuminuria and renal fibrosis in Zucker diabetic fatty (ZDF) rats. The experiments lasted 15 wk (from 5 to 20 wk of age) and were performed in 40 ZDF rats and 12 control lean rats. ZDF rats were divided into 4 groups: 1) 9 rats were treated with losartan; 2) 10 rats were treated with C21; 3) 9 rats were treated with losartan plus C21; and 4) 12 rats were maintained without any treatment. ZDF rats showed an increase in blood glucose level, albuminuria, renal fibrosis, macrophage infiltration, and TNF-α expression and a reduction of glomerular nephrin expression compared with control lean rats. C21 treatment reduced renal glomerular, tubulointerstitial, and perivascu-lar fibrosis, and macrophage infiltration and TNF-α expression in ZDF rats. C21 treatment caused a decrease in albuminuria in ZDF rats up to 11 wk of age. Losartan decreased macrophage infiltration, TNF-α expression, and renal glomerular and perivascular fibrosis, restored glomerular nephrin expression, but did not affect tubulointerstitial fibrosis. Losartan treatment caused a decrease in albuminuria in ZDF rats up to 15 wk of age. At the end of the protocol, only the combination of C21 plus losartan significantly reduced albuminuria in ZDF rats. These data demonstrate that C21 has beneficial effects on diabetic nephropathy, suggesting the combination of C21 and losartan as a novel pharmacological tool to slow the progression of nephropathy in type II diabetes. [ABSTRACT FROM AUTHOR] more...
- Published
- 2014
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30. Angiolymphoid Hyperplasia with Eosinophilia and Radial Artery Pseudoaneurysm.
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Petrakis, Ioannis E., Di Gioia, Cira R. T., and Sciacca, Vincenzo
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A case of angiolymphoid hyperplasia with eosinophilia arising from the radial artery is presented. Histologically, there was proliferation of atypical endothelial cells forming vascular spaces and solid cords, with a background infiltrate of inflammatory cells and prominent tissue eosinophilia. Immunohistochemical studies demonstrated vimentin and Factor VIII-related antigen in the endothelial cells. The lymphoid infiltrate was polyclonal. The authors discuss the probable nature of this process, which typically occurs in the dermis and subcutaneous tissue of the head and neck and is known by a variety of different names, reflecting disagreement regarding pathogenesis. This case draws attention to the fact that it can arise not only within the dermis and subcutaneous tissue of the head and neck, but also from major peripheral arteries. Angiolymphoid hyperplasia with eosinophilia should be considered promptly in the doubtful case of a pulsatile mass arising from peripheral arteries as it may lead to an earlier diagnosis and timely treatment with lower morbidity. [ABSTRACT FROM PUBLISHER] more...
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- 2000
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31. Angiotensin II Increases Calponin Expression in Cultured Rat Vascular Smooth Muscle Cells
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di Gioia, Cira R. T., van de Greef, Willy M. M., Sperti, Giovanni, Castoldi, Giovanna, Todaro, Nicoletta, Ierardi, Carolina, Pieruzzi, Federico, and Stella, Andrea
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Angiotensin II (Ang II) action on vascular smooth muscle cells is not limited to contraction, but includes long term effects such as hypertrophy and hyperplasia. This implies a complex pattern of gene modulation, which remains largely unknown. We used the mRNA differential display method to screen rat aortic smooth muscle cells cultured with or without Ang II. We demonstrated that Ang II induces the expression of calponin, a 34-kD protein, which has been shown to regulate smooth muscle cell contraction and to be a marker of smooth muscle cell differentiation. We demonstrated this induction both at gene and protein level in vascular smooth muscle cells. Calponin mRNA was dose-dependently induced by Ang II, with an effect still evident at 5 × 10−9M, and it did not require active protein synthesis, since cycloheximide treatment did not suppress this induction. Calponin gene expression was maximal at 3 h, while protein expression was maximal at 8 h. Calponin expression was completely abolished by the AT1 receptor antagonist, losartan, at 1 × 10−6M. Our data demonstrate that Ang II increases calponin gene expression and protein level in rat aortic smooth muscle cells in vitro. more...
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- 2000
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32. Effects of oral immunonutrition on histological changes of inflammatory infiltration of the tumor microenvironment among patients with a new diagnosis of gastric cancer.
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Molfino A, Mari A, Paldino A, Carletti R, Imbimbo G, Cardi M, di Gioia CRT, and Laviano A
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- Humans, Tumor Microenvironment, Nutritional Support, Dietary Supplements, Weight Loss, Stomach Neoplasms surgery
- Abstract
Objectives: We assessed the effects of oral immunonutrition (OI) on the inflammatory infiltration of the tumor microenvironment (TME) of patients undergoing surgery for gastric cancer., Methods: We analyzed patients at the time of their first gastric cancer diagnosis. We collected surgical tissue specimens (stomach), and performed an immunohistochemical analysis to evaluate the inflammatory infiltration of the TME. Patients receiving OI were compared with patients receiving standard oral nutritional supplements and no nutritional support., Results: We enrolled 12 patients with gastric cancer in the study. The median body weight loss was 5.6%. Four patients received OI, five patients received standard nutritional supplement, and three patients received no nutritional supplementation. No difference in age, body mass index (BMI), and body weight loss was observed between the three groups. The OI group showed a tendency of increased number of T-lymphocyte cluster of differentiation (CD) 8+ compared with the other groups, as well as the number of CD83+ and CD68+. The absence of F4/80+ cells was documented only in the TME of the OI group, where a linear positive correlation was present between lymphocytes CD4+ and CD8+ (R = 0.48), and between CD4+ and CD83+ (R = 0.89), although not statistically significant. In the OI group, we observed a positive correlation (not significant) between the number of lymphocytes CD8+ and macrophages CD68+ (R = 0.70; P = 0.30). A strong significant correlation was documented between CD68+ and CD40+ (R = 0.99; P = 0.01), but this correlation did not reach the significance among the patients of the other two groups (R = 0.60; P = 0.116)., Conclusions: The administration of OI in patients with gastric cancer might determine changes in inflammatory patterns of the TME., (Copyright © 2022 Elsevier Inc. All rights reserved.) more...
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- 2023
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33. Histomorphological and inflammatory changes of white adipose tissue in gastrointestinal cancer patients with and without cachexia.
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Molfino A, Carletti R, Imbimbo G, Amabile MI, Belli R, di Gioia CRT, Belloni E, Spinelli F, Rizzo V, Catalano C, Nigri G, and Muscaritoli M
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- Adipose Tissue pathology, Humans, Intra-Abdominal Fat metabolism, Subcutaneous Fat, Cachexia pathology, Gastrointestinal Neoplasms complications, Gastrointestinal Neoplasms pathology
- Abstract
Background: During cancer cachexia, several alterations occur in peripheral tissues, and the adipose tissue may be involved during the catabolic state. We aimed at investigating histological rearrangement and infiltration of inflammatory cells in subcutaneous adipose tissue (SAT) of patients with cancer undergoing surgery, according to the presence/absence of cachexia., Methods: We considered gastrointestinal cancer patients and controls with non-malignant diseases undergoing surgery. We collected SAT samples and performed histomorphological analyses [cross-sectional area (CSA) and per cent of fibrosis] and immunohistochemistry to characterize the inflammatory cells. By computed tomography (CT) scan, we calculated SAT and visceral adipose tissue (VAT)., Results: We enrolled 51 participants (31 gastrointestinal cancer patients and 20 controls). In cancer patients, cachexia was present in 13/31 (42%). The CSA (μm
2 ) of the adipocytes from SAT was reduced in cancer patients vs. controls (3148, inter-quartile range 2574-3755 vs. 4474, inter-quartile range 3654-5183) (P < 0.001), in particular in cachectic patients vs. non-cachectic (median 2518 vs. median 3470) (P = 0.03) and in cachectic vs. controls (P < 0.001), as well as in non-cachectic vs. controls (P = 0.04). The median per cent of fibrosis was higher in cancer patients vs. controls (9 vs. 3) (P = 0.0001), in particular in cachectic vs. non-cachectic (13.35 vs. 7.13) (P = 0.03). We observed a higher number of macrophages (CD68) (P = 0.0001) and T lymphocytes (CD3) (P = 0.002) in SAT of cancer patients vs. controls, and the number of T lymphocytes was higher in cachectic vs. non-cachectic patients (P = 0.025). Anorexic cancer patients showed in SAT a higher number of macrophages and T lymphocytes with respect to controls (P < 0.0001), whereas no difference was present between anorexic and non-anorexic patients. At CT scan, cachectic patients showed lower VAT and SAT vs. non-cachectic (VAT: 97.64 ± 40.79 vs. 212.53 ± 79.24, P = 0.0002; SAT: 126.27 ± 87.92 vs. 206.27 ± 61.93, P = 0.01, respectively). Cancer patients with low CSA, high degree of fibrosis, and high number of T lymphocytes presented with lower body mass index and lower SAT and VAT at CT scan (P ≤ 0.01)., Conclusions: We found histological alterations of SAT among gastrointestinal cancer patients and in particular significant changes in CSA, fibrosis, and inflammation when cachexia was present; the changes in histomorphological parameters of the adipocytes reflected alterations in adiposity at body composition analysis., (© 2021 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders.) more...- Published
- 2022
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34. Nonischemic left ventricular scar and cardiac sudden death in the young.
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di Gioia CR, Giordano C, Cerbelli B, Pisano A, Perli E, De Dominicis E, Poscolieri B, Palmieri V, Ciallella C, Zeppilli P, and d'Amati G
- Subjects
- Adolescent, Adult, Age Distribution, Autopsy, Biopsy, Cardiomyopathies genetics, Cardiomyopathies mortality, Cardiomyopathies physiopathology, Child, Child, Preschool, Cicatrix genetics, Cicatrix mortality, Cicatrix physiopathology, DNA Mutational Analysis, Death, Sudden, Cardiac epidemiology, Diagnosis, Differential, Electrocardiography, Female, Genetic Markers, Genetic Predisposition to Disease, Humans, Infant, Italy epidemiology, Male, Molecular Diagnostic Techniques, Mutation, Phenotype, Predictive Value of Tests, Prevalence, Risk Factors, Young Adult, Cardiomyopathies pathology, Cicatrix pathology, Death, Sudden, Cardiac pathology, Myocardium metabolism
- Abstract
Nonischemic left ventricular scar (NLVS) is a pattern of myocardial injury characterized by midventricular and/or subepicardial gadolinium hyperenhancement at cardiac magnetic resonance, in absence of significant coronary artery disease. We aimed to evaluate the prevalence of NLVS in juvenile sudden cardiac death and to ascertain its etiology at autopsy. We examined 281 consecutive cases of sudden death of subjects aged 1 to 35 years. NLVS was defined as a thin, gray rim of subepicardial and/or midmyocardial scar in the left ventricular free wall and/or the septum, in absence of significant stenosis of coronary arteries. NLVS was the most frequent finding (25%) in sudden deaths occurring during sports. Myocardial scar was localized most frequently within the left ventricular posterior wall and affected the subepicardial myocardium, often extending to the midventricular layer. On histology, it consisted of fibrous or fibroadipose tissue. Right ventricular involvement was always present. Patchy lymphocytic infiltrates were frequent. Genetic and molecular analyses clarified the etiology of NLVS in a subset of cases. Electrocardiographic (ECG) recordings were available in more than half of subjects. The most frequent abnormality was the presence of low QRS voltages (<0.5 mV) in limb leads. In serial ECG tracings, the decrease in QRS voltages appeared, in some way, progressive. NLVS is the most frequent morphologic substrate of juvenile cardiac sudden death in sports. It can be suspected based on ECG findings. Autopsy study and clinical screening of family members are required to differentiate between arrhythmogenic right ventricular cardiomyopathy/dysplasia and chronic acquired myocarditis., (Copyright © 2016 Elsevier Inc. All rights reserved.) more...
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- 2016
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35. MiR-133a regulates collagen 1A1: potential role of miR-133a in myocardial fibrosis in angiotensin II-dependent hypertension.
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Castoldi G, Di Gioia CR, Bombardi C, Catalucci D, Corradi B, Gualazzi MG, Leopizzi M, Mancini M, Zerbini G, Condorelli G, and Stella A
- Subjects
- Angiotensin II genetics, Animals, Collagen Type I genetics, Collagen Type I, alpha 1 Chain, Gene Expression Regulation physiology, Male, MicroRNAs genetics, Rats, Rats, Sprague-Dawley, Angiotensin II metabolism, Collagen Type I metabolism, Fibrosis metabolism, Heart Diseases metabolism, Hypertension metabolism, MicroRNAs metabolism
- Abstract
MicroRNAs play an important role in myocardial diseases. MiR-133a regulates cardiac hypertrophy, while miR-29b is involved in cardiac fibrosis. The aim of this study was to evaluate whether miR-133a and miR-29b play a role in myocardial fibrosis caused by Angiotensin II (Ang II)-dependent hypertension. Sprague-Dawley rats were treated for 4 weeks with Ang II (200 ng/kg/min) or Ang II + irbesartan (50 mg/kg/day in drinking water), or saline by osmotic minipumps. At the end of the experimental period, cardiac miR-133a and miR-29b expression was measured by real-time PCR, and myocardial fibrosis was evaluated by morphometric analysis. A computer-based prediction algorithm led to the identification of collagen 1a1 (Col1A1) as a putative target of miR-133a. A reporter plasmid bearing the 3'-untranslated regions (UTRs) of Col1A1 mRNA was constructed and luciferase assay was performed. MiR-133a suppressed the activity of luciferase when the reporter gene was linked to a 3'-UTR segment of Col1A1 (P < 0.01). Mutation of miR-133a binding sites in the 3'-UTR of Col1A1 mRNA abolished miR-133a-mediated repression of reporter gene activity, showing that Col1A1 is a real target of miR-133a. In vivo, Ang II caused an increase in systolic blood pressure (P < 0.0001, tail cuff) and myocardial fibrosis in presence of a decrease in miR-133a (P < 0.01) and miR-29b (P < 0.01), and an increase in Col1A1 expression (P < 0.01). These effects were abolished by Ang II administration + irbesartan. These data demonstrate a relationship between miR-133a and Col1A1, suggesting that myocardial fibrosis occurring in Ang II-dependent hypertension is regulated by the down-regulation of miR-133a and miR-29b through the modulation of Col1A1 expression., (Copyright © 2011 Wiley Periodicals, Inc.) more...
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- 2012
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36. Prevention of myocardial fibrosis by N-acetyl-seryl-aspartyl-lysyl-proline in diabetic rats.
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Castoldi G, di Gioia CR, Bombardi C, Perego C, Perego L, Mancini M, Leopizzi M, Corradi B, Perlini S, Zerbini G, and Stella A
- Subjects
- Angiotensin-Converting Enzyme Inhibitors administration & dosage, Animals, Cardiomyopathies prevention & control, Fibrosis, Heart Ventricles drug effects, Hypoglycemic Agents administration & dosage, Insulin administration & dosage, Male, Oligopeptides administration & dosage, Ramipril administration & dosage, Rats, Rats, Sprague-Dawley, Smad Proteins drug effects, Smad Proteins metabolism, Transforming Growth Factor beta1 drug effects, Transforming Growth Factor beta1 metabolism, Diabetes Complications prevention & control, Diabetes Mellitus, Experimental physiopathology, Heart Ventricles pathology, Myocardium pathology, Oligopeptides pharmacology
- Abstract
Ac-SDKP (N-acetyl-seryl-aspartyl-lysyl-proline) is a physiological tetrapeptide hydrolysed by ACE (angiotensin-converting enzyme). In experimental models of hypertension, Ac-SDKP has antifibrotic effects in the heart; however, the role of Ac-SDKP in diabetic cardiomyopathy is currently unknown. The aim of the present study was to evaluate the effect of Ac-SDKP on cardiac systolic and diastolic function, and interstitial and perivascular fibrosis in the heart of diabetic rats.Diabetes was induced in 55 Sprague-Dawley rats by streptozotocin injection. Control rats (n=18)underwent only buffer injection.Out of the 55 diabetic rats, 19 were chronically treated with insulin and 13 with the ACEI (ACE inhibitor) ramipril (3 mg x kg(-1 )of body weight x day(-1)). At 2 months after the onset of diabetes, Ac-SDKP (1 mg x kg(-1) of body weight x day(-1)) was administered by osmotic minipumps for 8 weeks to eight control rats, 13 diabetic rats, seven diabetic rats treated with ramipril and nine insulin-treated diabetic rats. Diabetic rats had a significant increase in blood glucose levels. Left ventricular interstitial and perivascular fibrosis, and TGF-beta1 (transforming growth factor-beta1) protein levels were increased in diabetic rats, but not in insulin-treated diabetic rats and ramipril-treated diabetic rats, compared with control rats. Ac-SDKP administration significantly reduced left ventricular interstitial and perivascular fibrosis in diabetic rats and in diabetic rats treated with ramipril. This was accompanied by a significant reduction in active TGF-beta1 and phospho-Smad2/3 protein levels in myocardial tissue of diabetic rats. Echocardiography showed that diabetes was associated with increased end-systolic diameters, and depressed global systolic function and diastolic dysfunction, as assessed by transmitral Doppler velocity profile. These changes were completely reversed by insulin or ramipril treatment. Ac-SDKP treatment partially restored diastolic function in diabetic rats. In conclusion, Ac-SDKP administration in diabetic rats reduces left ventricular interstitial and perivascular fibrosis, active TGF-beta1 and phospho-Smad2/3levels, and improves diastolic function. Taken together, these findings suggest that, by inhibiting theTGF-beta/Smad pathway, Ac-SDKP protects against the development of diabetic cardiomyopathy more...
- Published
- 2009
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37. [Three good reasons to perform a postmortem examination in all cases of juvenile sudden death].
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d'Amati G, di Gioia CR, Silenzi PF, and Gallo P
- Subjects
- Adolescent, Age Factors, Death, Sudden etiology, Female, Humans, Male, Pedigree, Autopsy, Death, Sudden pathology
- Abstract
The aim of this review is to underline the reasons why a post-mortem examination has to be performed in all cases of juvenile sudden death. Sudden death in children and young adults can be caused by potentially heritable cardiovascular disorders and fatal outcome is often the first symptom in apparently healthy subjects. In these cases, a careful autopsy, performed according to a standardized protocol, becomes the sole diagnostic tool to guide clinical and molecular genetic family screening and to adopt the proper therapeutic and preventive strategies. Thus, a post-mortem examination is a fundamental part of a multidisciplinary approach to the issue of juvenile sudden death. more...
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- 2009
38. Blue rubber bleb nevus syndrome and pulmonary hypertension: an unusual association.
- Author
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Giordano C, Battagliese A, di Gioia CR, Campagna D, Benedetti F, Travaglini C, Gallo P, and d' Amati G
- Subjects
- Adult, Arteriovenous Malformations pathology, Fatal Outcome, Humans, Hypertension, Pulmonary pathology, Male, Skin blood supply, Syndrome, Thromboembolism complications, Thromboembolism pathology, Arteriovenous Malformations complications, Hypertension, Pulmonary complications, Neoplasms pathology, Nevus, Blue pathology, Skin Neoplasms pathology
- Abstract
Introduction: Blue rubber bleb nevus syndrome (BRBNS) is a rare congenital systemic angiodysplasia with multiple vascular malformations in the skin, gastrointestinal tract and, less often, in other internal organs and the brain., Case Report: A 36-year-old man with past history of BRBNS was admitted to our hospital for progressive dyspnea and fatigue. Primary pulmonary hypertension (PPH) was diagnosed. He then developed acute abdominal pain and dyspnea, dying in a few hours due to sudden cardiac arrest. Postmortem examination demonstrated angiomatous lesions located in the skin, small bowel, heart, lungs, liver and thyroid. The lesions were slightly raised, soft and compressible and microscopically consisted of dilated vascular channels lined by a flattened endothelium. The vascular wall was formed by several layers of smooth muscle cells, intermixed with abundant aggregates of elastic lamellae and thin collagen fibers. Luminal thrombi were a frequent finding. In the small bowel, we identified the presence of an abnormally large artery directly opening into a thin-walled venous channel. The most striking finding in the lungs was the presence of thrombi of varying age in the lumen of segmental and elastic arteries, as well as muscular arteries and arterioles. Severe medial hypertrophy of muscular arteries and muscolarization of arterioles were also present. Intimal proliferative lesions and plexiform lesions were never observed., Conclusion: The pulmonary findings are consistent with recurrent thromboembolic events from shunts in the visceral lesions. To our knowledge, this is the first report of BRBNS with visceral arterovenous (AV) fistulae complicated by thromboembolic pulmonary hypertension (PH). more...
- Published
- 2004
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39. Detection of deleted mitochondrial DNA in Kearns-Sayre syndrome using laser capture microdissection.
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Pistilli D, di Gioia CR, D'Amati G, Sciacchitano S, Quaglione R, Quitadamo R, Casali C, Gallo P, and Santorelli FM
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- Adult, DNA, Mitochondrial analysis, Electron Transport Complex IV genetics, Electron Transport Complex IV metabolism, Female, Humans, Kearns-Sayre Syndrome enzymology, Muscle Fibers, Skeletal enzymology, Muscle Fibers, Skeletal pathology, Muscle, Skeletal enzymology, Muscle, Skeletal pathology, Polymerase Chain Reaction, DNA, Mitochondrial genetics, Gene Deletion, Kearns-Sayre Syndrome genetics, Laser Therapy, Microdissection methods
- Abstract
A novel 4949-base pair mitochondrial DNA (mtDNA) deletion was detected in various tissues in a postmortem study of a patient with Kearns-Sayre syndrome (KSS). Deleted mtDNA levels were higher in skeletal muscle and brain and lower in kidney, working myocardium, and endocrine tissues (thyroid, parathyroids, pancreas, and adrenal glands). The distribution of the deletion in skeletal muscle and conducting myocardium was analyzed by means of laser capture microdissection (LCM). In skeletal muscle, the abundance of deleted mtDNA was slightly higher in cytochrome c oxidase (COX)-negative fibers (70%) than in COX-positive fibers (64%), whereas in the conducting myocardium it was lower in the atrioventricular node (9%) than in the sinus node and bundle of His (30% and 32%, respectively). In this study, LCM proved to be a reliable technique for a more accurate assessment of genotype/phenotype correlation when investigating mtDNA-related disorders. more...
- Published
- 2003
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40. Integrated approach for cardiac angiosarcoma.
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Sinatra R, Brancaccio G, di Gioia CR, De Santis M, Sbraga F, and Gallo P
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- Fatal Outcome, Female, Humans, Middle Aged, Delivery of Health Care, Integrated, Heart Neoplasms diagnosis, Heart Neoplasms therapy, Hemangiosarcoma diagnosis, Hemangiosarcoma therapy
- Published
- 2003
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41. ANG II increases TIMP-1 expression in rat aortic smooth muscle cells in vivo.
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Castoldi G, Di Gioia CR, Pieruzzi F, D'Orlando C, Van De Greef WM, Busca G, Sperti G, and Stella A
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- Angiotensin II administration & dosage, Animals, Aorta cytology, Cells, Cultured, Dose-Response Relationship, Drug, Drug Administration Schedule, Infusion Pumps, Male, Muscle, Smooth, Vascular cytology, RNA, Messenger metabolism, Rats, Rats, Sprague-Dawley, Tissue Inhibitor of Metalloproteinase-1 genetics, Angiotensin II pharmacology, Aorta metabolism, Muscle, Smooth, Vascular metabolism, Tissue Inhibitor of Metalloproteinase-1 metabolism
- Abstract
Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are involved in tissue remodeling processes. TIMP-1 is the main native inhibitor of MMPs and it contributes to the development of tissue fibrosis. It is known that ANG II plays a fundamental role in vascular remodeling. In this study, we investigated whether ANG II modulates TIMP-1 expression in rat aortic smooth muscle cells. In vitro, ANG II induces TIMP-1 mRNA expression in a dose-dependent manner. The maximal increase in TIMP-1 expression was present after 3 h of ANG II stimulation. The ANG II increase in TIMP-1 expression was mediated by the ANG type 1 receptors because it was blocked by losartan. The increase in TIMP-1 expression was present after the first ANG II treatment, whereas repeated treatments (3 and 5 times) did not modify TIMP-1 expression. In vivo, exogenous ANG II was administered to Sprague-Dawley rats (200 ng. kg(-1). min(-1) sc) for 6 and 25 days. Control rats received physiological saline. After treatment, systolic blood pressure was significantly higher (P < 0.01), whereas plasma renin activity was suppressed (P < 0.01), in ANG II-treated rats. ANG II increased TIMP-1 expression in the aorta of ANG II-treated rats both at the mRNA (P < 0.05) and protein levels as evaluated by Western blotting (P < 0.05) and/or immunohistochemistry. Neither histological modifications at the vascular wall nor differences in collagen content in the tunica media were present in both the ANG II- and saline-treated groups. Our data demonstrate that ANG II increases TIMP-1 expression in rat aortic smooth muscle cells. In vivo, both short- and long-term chronic ANG II treatments increase TIMP-1 expression in the rat aorta. TIMP-1 induction by ANG II in aortic smooth muscle cells occurs in the absence of histological changes at the vascular wall. more...
- Published
- 2003
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42. Type 5 phosphodiesterase expression in the human vagina.
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D'Amati G, di Gioia CR, Bologna M, Giordano D, Giorgi M, Dolci S, and Jannini EA
- Subjects
- 3',5'-Cyclic-GMP Phosphodiesterases, Adult, Blotting, Western, Clitoris blood supply, Clitoris enzymology, Clitoris metabolism, Cyclic Nucleotide Phosphodiesterases, Type 5, Epithelium blood supply, Epithelium enzymology, Epithelium metabolism, Female, Humans, Immunohistochemistry, Male, Muscle, Smooth, Vascular chemistry, Muscle, Smooth, Vascular enzymology, Muscle, Smooth, Vascular physiology, Nitric Oxide Synthase metabolism, Nitric Oxide Synthase physiology, Phosphodiesterase Inhibitors therapeutic use, Phosphoric Diester Hydrolases biosynthesis, Phosphoric Diester Hydrolases physiology, Sex Factors, Sexual Behavior physiology, Sexual Dysfunctions, Psychological drug therapy, Tissue Distribution, Vagina blood supply, Vagina metabolism, Phosphoric Diester Hydrolases analysis, Vagina enzymology
- Abstract
Objectives: It has been demonstrated that clitoral and vaginal tissues express nitric oxide synthase isoforms in a way that parallels that of the penile corpus cavernosum. Considering the role of the vagina in the female sexual response and the anatomic connection between the clitoris and the anterosuperior vaginal wall, our aim was to study the distribution of type 5 phosphodiesterase (PDE5) in the anterosuperior wall of the human vagina., Methods: Immunohistochemistry was performed on the vaginal tissue of 14 women obtained at autopsy and on exfoliated cells of the vaginal epithelium obtained from 5 healthy female donors. Specific antibodies against PDE5 were tested on both paraffin sections and cytologic smears. Immunoblotting experiments were performed in parallel with the same antibodies., Results: The histologic analysis of human cadaveric vaginal tissue revealed that PDE5 immunoreactivity was mostly localized in the smooth muscle of vessels, forming a pseudocavernous tissue in the vaginal wall and endothelium. The Skene periurethral glands and vaginal epithelium were also positive for the antibody. The latter finding was confirmed using exfoliated cells of the vaginal epithelium harvested in vivo., Conclusions: The presence and tissue distribution of PDE5 in the human vagina suggest that the integrated system of nitric oxide synthase-PDE5 may play a physiologic role not only in the male sexual response but also in female sexual arousal. more...
- Published
- 2002
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43. Cardinal vein isomerism: an embryological hypothesis to explain a persistent left superior vena cava draining into the roof of the left atrium in the absence of coronary sinus and atrial septal defect.
- Author
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Miraldi F, di Gioia CR, Proietti P, De Santis M, d'Amati G, and Gallo P
- Subjects
- Adult, Female, Heart Septal Defects, Atrial surgery, Humans, Magnetic Resonance Angiography, Treatment Outcome, Coronary Vessel Anomalies pathology, Heart Atria abnormalities, Heart Septal Defects, Atrial pathology, Vena Cava, Superior abnormalities
- Abstract
Background: A persistent left superior vena cava (PLSVC) is a relatively frequent systemic venous anomaly associated with congenital heart defects. This anomaly has been explained with the persistence of the left superior cardinal vein. PLSVC usually drains into the right atrium, via coronary sinus, but it joins the left atrium in approximately 8% of the cases either directly in the setting of atrial isomerism, or via an unroofed coronary sinus, or through a coronary sinus type atrial septal defect., Case Report: We describe a case of an adult patient with atria in the situs solitus, PLSVC draining into the left atrium, atresia of coronary sinus without atrial septal defect, and with additional cardiac anomalies (ventricular septal defect and discrete subaortic stenosis)., Conclusion: A possible embryological explanation to this case rises from a right partial isomerism of the superior cardinal veins, which gives reason for both the coexistence of the PLSVC draining into the left atrium and the absence of coronary sinus, atrial septal defect, or coronary sinus ostium. more...
- Published
- 2002
- Full Text
- View/download PDF
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