48 results on '"differential metabolite"'
Search Results
2. Metabolic Response of the Lycium barbarum Variety 'Ningqi No. 7′ to Drought Stress.
- Author
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Liu, Xiao, Wang, Chuanzhe, Xu, Qiao, Zhao, Dan, Liu, Fei, and Han, Beibei
- Subjects
LIQUID chromatography-mass spectrometry ,PLANT enzymes ,VITAMIN B6 ,ENERGY dissipation ,ENERGY metabolism ,DROUGHT tolerance - Abstract
Lycium barbarum has been widely planted in arid and semi-arid areas due to its drought-resistant ability, which is of great economic value as a medicinal and edible homology plant. In this study, the metabolome of the L. barbarum variety "Ningqi 7" under different drought stress conditions was compared and analyzed by the non-targeted UPLC-MS (ultra-high performance liquid chromatography with mass spectrometry) technique. The results showed that drought stress significantly decreased the water content of leaves, increased the activity of antioxidant enzymes in plants, and up-regulated the metabolites and pathways involved in osmoregulation, antioxidant stress, energy metabolism, and signal transduction. Under moderate drought (40–45% FC), L. barbarum accumulated osmoregulatory substances mainly through the up-regulation of the arginine metabolism pathway. At the same time, phenylalanine metabolism and cutin, suberine, and wax biosynthesis were enhanced to improve the antioxidant capacity and reduce water loss. However, in severe drought (10–15% FC), L. barbarum shifted to up-regulate purine metabolism and lysine degradation and redistributed energy and nitrogen resources. In addition, vitamin B6 metabolism was significantly upregulated in both groups of stress levels, playing a key role in antioxidant and growth regulation. These observations delineate the metabolic adaptations of L. barbarum "Ningqi 7" in response to drought stress. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. 基于非靶向代谢组学分析两种酵母培养物的成分差异.
- Author
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林青, 时红玲, 秦新政, 李月, 王子涵, 高雁, 曾军, 王浩中, 娄恺, and 霍向东
- Abstract
Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
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- View/download PDF
4. Lipid degradation contributes to flavor formation during air-dried camel jerky processing
- Author
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Shenyi Cao and Yinghua Fu
- Subjects
Air-dried camel jerky ,Lipidomics ,Volatile compounds ,Key aroma compounds ,Differential metabolite ,Correlation analysis ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
Lipids play an important role in flavor formation in meat products. To determine the contribution of lipids to flavor formation during air-dried camel jerky processing, lipid changes were analyzed by UHPLC-Q-Exactive Orbitrap MS/MS in this study, and volatile compounds were identified by HS-SPME-GC-ToF-MS. Results showed that 606 lipid molecules belonging to 30 subclasses were identified and 206 differential lipid molecules were screened out (VIP > 1, P
- Published
- 2024
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- View/download PDF
5. 腐植酸负载纳米零价铁对镉污染农田水稻籽粒代谢的影响.
- Author
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黄鹏, 张静, 王鸿辉, 井江心, 曾燕琼, 陈铮, and 刘洋之
- Abstract
Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
- Full Text
- View/download PDF
6. 基于核磁共振和化学计量学鉴别啤酒类别.
- Author
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符艳美, 张祎迒, 王道兵, 冯 迪, 罗静仪, and 钟其顶
- Abstract
Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
7. 叶面喷施镁对紫米产质量、花青素及代谢物 含量的影响.
- Author
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杜雪丽, 柯璐瑶, 柳 展, 代明珠, 周 宇, 李剑兴, and 徐玖亮
- Abstract
Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
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8. Differences in Bioactive Composition of Sea Buckthorn Berry Pulp during the Ripening Process as Analyzed by Metabolomics Based on Ultra-high Performance Liquid Chromatography Coupled to Mass Spectrometry
- Author
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DING Jian, RUAN Chengjiang, YANG Hong, GUAN Ying, LI He, CHEN Ye, TANG Ke, WU Liren
- Subjects
sea buckthorn ,berry pulp ,differential metabolite ,lipid ,flavonoid ,Food processing and manufacture ,TP368-456 - Abstract
In order to reveal the differences in the biosynthesis and metabolism of the major bioactive composition in sea buckthorn berry pulp during ripening, non-targeted metabolomics based on ultra-high performance liquid chromatography coupled to mass spectrometry (UPLC-MS) was used to analyze the metabolites of sea buckthorn berry pulp from two strains at different developmental stages (green and orange pulp). The results showed that the four sample groups had significant metabolic differences. A total of 124 significantly differential metabolites were detected among the four groups based on variable importance in the projection (VIP) > 1 and t-test P < 0.05, including organic acids, carbohydrates, lipids, flavonoids and secondary metabolites. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, 15, 7 and 4 significantly differential metabolites associated with lipid, flavonoid and serotonin metabolism were identified, respectively, and eight significantly differential metabolites involved in anticancer function were selected. The significant up-regulation of phosphatidylcholine (PC) (16:0/16:0) and glycerophosphocholine could be the reason for the high accumulation of lipids in sea buckthorn berry pulp. The significant upregulation of isorhamnetin was key for the high flavonoid content in sea buckthorn berry pulp. Relatively high contents of N-acetylserotonin, capecitabine, γ-aminobutyric acid (GABA) and other bioactive composition involved in anticancer function were shown in sea buckthorn berry pulp during its development. These results provide a scientific basis for revealing the metabolic mechanism of bioactive composition of sea buckthorn berries and developing related functional products.
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- 2023
- Full Text
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9. A Comparative Metabolomics Study of Glucosinolates in Four Watercress (Nasturtium officinale R. Br.) Samples from Different Origins Using Ultra-High-Performance Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry
- Author
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Suchong Deng, Xiaoqing Ma, Qiang Ding, and Xilin Hou
- Subjects
Nasturtium officinale R. Br. ,glucosinolate ,UHPLC-ESI-MS/MS ,metabolomics ,differential metabolite ,Plant culture ,SB1-1110 - Abstract
Glucosinolates are important plant secondary metabolites that are involved in plant defense responses and have beneficial effects on human life and health. Watercress (Nasturtium officinale R. Br.) is an aquatic vegetable rich in glucosinolates. This study utilized ultra-high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (UHPLC-ESI-MS/MS) to compare and analyze four watercress samples from different origins. A total of 35 glucosinolates were identified, including 33 differential glucosinolates, and their relative content in different samples was determined. Differential metabolite screening revealed significant differences in the watercress from Guangdong, China (GD), compared with other samples. It contained two specific glucosinolates: 2(R)-Hydroxy-2-Phenylethyl glucosinolate and 4-Benzoyloxybutyl glucosinolate. The watercress from the United States of America (MG) had the highest total glucosinolate content. Genes involved in glucosinolate biosynthesis, such as NoCYP83A1 and NoSUR1, were also expressed differently in the four samples. This study provides a reference for further investigation of glucosinolate metabolites in the four watercress samples and the utilization of glucosinolates in watercress.
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- 2024
- Full Text
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10. 基于UPLC-MS技术分析沙棘果肉成熟过程中 生物活性成分差异.
- Author
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丁 健, 阮成江, 杨 红, 关 莹, 李 贺, 陈 叶, 唐 克, and 吴立仁
- Subjects
SEA buckthorn ,FLAVONOIDS ,BERRIES ,LIPIDS - Abstract
Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
11. Analysis of Differential Metabolites of Phyllostachys edulis Shoots at Different Growth Stages by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
- Author
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GUO Mingyang, HE Yuelin, PAN Kaiting, BAO Fangyan, YING Yeqing
- Subjects
phyllostachys edulis shoots ,different growth stages ,metabolomics ,differential metabolite ,nutritional quality ,Food processing and manufacture ,TP368-456 - Abstract
The dynamic changes of differential metabolites of Phyllostachys edulis shoots at three stages including ground breaking (S1, 5 cm high), growth (S2, 20 cm high) and rapid growth (S3, 40 cm high) were studied by widely targeted metabolomics combined with basic physiological indices. The results of physiological analyses showed that the nutritional and flavor quality was better at the ground breaking stage than at the other growth stages. A total of 765 metabolites were identified by metabolomics. Multivariate statistical analysis showed that the composition of metabolites varied significantly among growth stages. There were 203 differentially accumulated metabolites identified, among which lipids, amino acids, organic acids, phenolic acids, flavonoids and alkaloids remarkably changed. The nutritional quality at the ground breaking stage could be closely related to the enrichment of most flavonoids, free fatty acids, amino acids and neochlorogenic acid. At the latter two stages, bamboo shoots mainly contained lipids, amino acid derivatives, organic acids, phenolic acids and melatonin as well as a large number of bioactive ingredients, which could be closely related to improving the environmental adaptability of bamboo shoots. The results of this study provide a theoretical basis for understanding the nutritional value of P. edulis shoots and for the exploitation and utilization of bamboo resources.
- Published
- 2023
- Full Text
- View/download PDF
12. Metabolic Response of the Lycium barbarum Variety ‘Ningqi No. 7′ to Drought Stress
- Author
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Xiao Liu, Chuanzhe Wang, Qiao Xu, Dan Zhao, Fei Liu, and Beibei Han
- Subjects
Lycium barbarum ,UPLC-MS ,differential metabolite ,drought resistance mechanism ,Botany ,QK1-989 - Abstract
Lycium barbarum has been widely planted in arid and semi-arid areas due to its drought-resistant ability, which is of great economic value as a medicinal and edible homology plant. In this study, the metabolome of the L. barbarum variety “Ningqi 7” under different drought stress conditions was compared and analyzed by the non-targeted UPLC-MS (ultra-high performance liquid chromatography with mass spectrometry) technique. The results showed that drought stress significantly decreased the water content of leaves, increased the activity of antioxidant enzymes in plants, and up-regulated the metabolites and pathways involved in osmoregulation, antioxidant stress, energy metabolism, and signal transduction. Under moderate drought (40–45% FC), L. barbarum accumulated osmoregulatory substances mainly through the up-regulation of the arginine metabolism pathway. At the same time, phenylalanine metabolism and cutin, suberine, and wax biosynthesis were enhanced to improve the antioxidant capacity and reduce water loss. However, in severe drought (10–15% FC), L. barbarum shifted to up-regulate purine metabolism and lysine degradation and redistributed energy and nitrogen resources. In addition, vitamin B6 metabolism was significantly upregulated in both groups of stress levels, playing a key role in antioxidant and growth regulation. These observations delineate the metabolic adaptations of L. barbarum “Ningqi 7” in response to drought stress.
- Published
- 2024
- Full Text
- View/download PDF
13. 基于UPLC-MS/MS的毛竹笋不同生长 阶段差异代谢物分析.
- Author
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郭明阳, 贺曰林, 潘凯婷, 鲍方艳, and 应叶青
- Subjects
AMINO acid derivatives ,PHENOLIC acids ,MULTIVARIATE analysis ,FREE fatty acids ,AMINO acids ,ORGANIC acids - Abstract
Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
14. 日粮精粗比对藏系绵羊精液代谢产物的影响.
- Author
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吴振岭, 周力, 张峰硕, 侯生珍, and 桂林生
- Abstract
The experiment was to elucidate the effect of dietary concentrate to roughage ratio on semen metabolites of plateau Tibetan ram. Fifteen plateau Tibetan rams with good growth and similar body weight (26.58 ± 1.07) kg were selected and randomly divided into three groups, with five replicates in each group and one ram in each replicate. Rams in each group were fed with diets concentrate to roughage ratio of 70∶30 (HM group), 50∶50 (MM group) and 30∶70 (LM group) respectively. Pre-test period was 15 d and trial period was 90 d. The results showed that with the gradual increase of concentrate proportion in diet, the average sperm collection, sperm motility and sperm density of plateau Tibetan sheep were increased to varying degrees. The average sperm motility and sperm density of rams were the best when concentrate to roughage ratio was 70∶30. Rams fed a 50∶50 concentrate to roughage diet had the highest mean sperm intake. A total of 1 954 differential metabolites were screened by two-by-two comparisons between the groups of this histological data. 718 were obtained between HM group and LM group, of which 278 were up-regulated ions and 440 down-regulated ions. 855 were obtained between MM group and LM group, of which 446 were up-regulated ions and 409 down-regulated ions. 381 were obtained between HM group and LM group, of which 147 were up-regulated ions and 234 were down-regulated ions. The enrichment pathways of KEGG analysis mainly include purine metabolism, pentose phosphate pathway, fatty acid biosynthesis, prolactin signaling pathway, amino sugar and nucleotide sugar metabolism, etc. Glycine, glucose, acetyl-CoA, ADP and alpha-D-galactose were selected as the key differential metabolites. The content of glycine in semen of Tibetan sheep in HM group was significantly lower than that in LM group and MM group (P<0.05), and the content of other four key metabolites in semen of Tibetan sheep in HM group were significantly higher than that in LM group and MM group (P<0.05). The study indicates that the change of dietary concentrate to roughage ratio can significantly affect the metabolism and quality of semen of plateau Tibetan sheep, and concentrate to roughage ratio of 70∶ 30 has the best effect. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
15. 慢性肌筋膜触发点模型大鼠的尿液代谢组学分析.
- Author
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刘 琳, 刘世轩, 陆馨悦, and 王 侃
- Subjects
- *
MYOFASCIAL pain syndromes , *LABORATORY rats , *LIQUID chromatography-mass spectrometry , *ARACHIDONIC acid , *RUNNING speed , *TREADMILL exercise - Abstract
BACKGROUND: Chronic myofascial trigger points can identify differential metabolite changes through non targeted metabolomics techniques, helping to understand and further explore the pathophysiological processes and pathogenesis of chronic myofascial trigger points from the perspective of endogenous small molecule metabolites. OBJECTIVE: To investigate potential biomarkers and related metabolic pathways based on urine metabolomics in the rat model of chronic myofascial trigger points. METHODS: Sixteen Sprague-Dawley rats were randomly divided into a model group and a normal group. The model group was used to establish a chronic myofascial trigger point animal model by combining blunt hitting with centrifugal exercise (treadmill slope: -16°, running speed: 16 m/min, training time: 90 minutes each), once a week for 8 continuous weeks, with 4 weeks off. After 12 weeks of modeling, the metabolic cage method was used to collect urine from rats at 24 hours after modeling. Liquid chromatography-mass spectrometry non-targeted metabolomics technology was used to detect metabolic profiles in the urine samples, screen common differential metabolites, and conduct bioinformatics analysis. RESULTS AND CONCLUSION: Compared with the normal group, there were 32 differential metabolic markers in the model group, of which 21 were upregulated and 11 were downregulated. A total of 14 differential metabolites were identified as potential biomarkers based on the value of variable important in projection greater than 3. The enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes indicated that the formation of chronic myofascial trigger points is closely related to metabolic pathways such as primary bile acid biosynthesis and arachidonic acid metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2025
- Full Text
- View/download PDF
16. Metabolomics Comparative Analysis on Tender Tips of White Sechium edule and Jiangtun Cyan Sechium edule
- Author
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Shaoqi SHI, Jintian CHEN, Yu'e LIN, Guoyuan XIE, Meihua HUANG, and Min WANG
- Subjects
sechium edule ,metabolomics ,differential metabolite ,kegg ,breeding ,Agriculture - Abstract
【Objective】In order to find out the substances that affect the quality and taste of Chaylte Vine, the tender tips of white and Jiangtun cyan Sechium edule were used for metabonomic analysis.【Method】Metabolomic detection was carried out for tender tips of the two Sechium edule by using ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS), and MetaboAnalyst 5.0, SIMCA-P and Origin software were used to analyze metabolome data.【Result】The results showed that there were significant differences in metabolites among tender tips of the two Sechium edule. A total of 135 differential metabolites were screened, including 82 down-regulated metabolites and 53 up-regulated metabolites. The cluster analysis of differential metabolites showed that the content of myrosinaldehyde in white Sechium edule was high, followed by coniferol. The lysophosphatidyl ethanolamine was the highest differential metabolite in Jiangtun cyan Sechium edule, followed by lysophosphatidylcholine. The result of KEGG shows that differential metabolites enriched in 5 major metabolic pathways, including purine, linoleic acid, flavonoid biosynthesis, synthesis of secondary metabolites and α-linolenic acid metabolism.【Conclusion】The lipid, flavonoid, alkaloids and nucleotides substances in the tender tips of Jiangtun cyan Sechium edule are high, especially lysophosphatidyl ethanolamine, daidzein, adenosine triphosphate and ferulic spermidine etc. The research results elucidated the nutritional and medicinal basis of the metabolomics of Jiangtun cyan Sechium edule, and provided a theoretical basis for the selection and breeding of high-quality varieties of Chaylte Vine.
- Published
- 2023
- Full Text
- View/download PDF
17. Effect of Different Accumulative Temperate Zones in Heilongjiang on Glycine Soja Metabolites as Analyzed by Non-Target Metabolomics.
- Author
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Bao, Guofeng, Mu, Liqiang, and Wang, Ying
- Subjects
- *
AMINO acid analysis , *ORGANIC acids , *MULTIVARIATE analysis , *METABOLOMICS , *METABOLITES , *AMINO acid metabolism - Abstract
To study the effect of growth temperature on the nutritional components and metabolites of the wild soybean (Glycine soja), we analyzed the nutritional components and metabolic gases of the wild soybean in six accumulated temperature regions of the Heilongjiang Province, China, by gas chromatography–time-of-flight mass spectrometry (GC-TOF-MS). A total of 430 metabolites, including organic acids, organic oxides, and lipids, were identified and analyzed using multivariate statistical analysis, orthogonal partial least squares discriminant analysis, principal component analysis, and cluster analysis. Eighty-seven metabolites significantly differed in the sixth accumulated temperature region compared with the other five accumulated temperature regions. The 40 metabolites (such as threonine (Thr) and lysine (Lys)) were found to be elevated in soybeans from the sixth accumulated temperature zone compared with the other five accumulated temperature zones. Through analyzing the metabolic pathways of these metabolites, amino acid metabolism had the greatest influence on wild soybean quality. The results of the amino acid analysis were consistent with those of the GC-TOF-MS and showed that amino acids in wild soybeans from the sixth accumulated temperature zone significantly differed from those of the other zones. Threonine and lysine were the main substances driving these differences. The growth temperature affected the type and concentrations of metabolites in wild soybeans, and the GC-TOF-MS analysis of the effect of growth temperature on wild soybean metabolites was shown to be feasible. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
18. 基于非靶向代谢组学的片烟醇化进程判断及 代谢物差异分析.
- Author
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梁珂馨, 奚家勤, 陈若星, 牟文君, 刘萍萍, 郑庆霞, 王得强, 贾中林, 李晓闯, and 张艳玲
- Abstract
Copyright of Tobacco Science & Technology is the property of Tobacco Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
19. 基于代谢组学技术的金刚烷胺胁迫刺参的毒性作用机 制研究.
- Author
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赵军强, 韩典峰, 田秀慧, 刘鸽, 刘小静, 高永刚, 姜芳, 刘欢, 崔艳梅, 罗晶晶, 洪赫阳, and 徐英江
- Subjects
AMINO acid metabolism ,APOSTICHOPUS japonicus ,CHOLESTEROL metabolism ,ADENOSINE monophosphate ,SEA cucumbers ,ENERGY metabolism - Abstract
Copyright of Asian Journals of Ecotoxicology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
20. '重瓣红'玫瑰不同花发育阶段转录和代谢差异分析.
- Author
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赵艳侠, 张晶莹, 孙骏飞, 王绛辉, 孙家波, and 吕晓惠
- Abstract
In order to explore the key genes and metabolic pathways regulating floral aroma during the development of‘Rose rugosa cv. Plena’, transcriptome and metabolome sequencing were performed on the samples at different development stages of flowers. The 4435, 2444, 7021 and 4123 differentially expressed genes in the comparison of flower bud stage, early opening stage, half opening stage, full opening stage and falling stage respectively were detected via transcriptomics analysis, of which 214 were differentially expressed genes in five stages. A total of 508 metabolites and 230 differential metabolites were detected by metabonomics. There were significant differences in metabolites at different developmental stages, and the metabolites were divided into 4 clusters. The 58 differentially expressed genes and 11 differentially expressed metabolites were found in the analysis of synthetic pathways of phenylpropanoid and terpene in‘Rosa rugosa’. Combined with transcriptome and metabolome analysis, the genes and volatile substances changed significantly during flower development. Monoterpenoids were formed in flower bud stage, volatiles gradually accumulated in half opening stage, and the release of metabolites decreased in falling stage. Typical rose aroma substances were mainly formed in half opening stage. Therefore, choosing the appropriate harvest time is conducive to the formation and retention of rose fragrance quality. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
21. 白皮和江屯青皮佛手瓜嫩梢的代谢组比较分析.
- Author
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史绍琪, 陈金甜, 林毓娥, 谢国源, 黄美华, and 王敏
- Subjects
- *
TANDEM mass spectrometry , *METABOLITES , *ADENOSINE triphosphate , *LINOLEIC acid , *FLAVONOIDS , *LINOLENIC acids - Abstract
[Objective] In order to find out the substances that affect the quality and taste of Chaylte Vine, the tender tips of white and Jiangtun cyan Sechium edule were used for metabonomic analysis.[Method] Metabolomic detection was carried out for tender tips of the two Sechium edule by using ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS), and MetaboAnalyst 5.0, SIMCA-P and Origin software were used to analyze metabolome data. [Result] The results showed that there were significant differences in metabolites among tender tips of the two Sechium edule. A total of 135 differential metabolites were screened, including 82 down-regulated metabolites and 53 up-regulated metabolites. The cluster analysis of differential metabolites showed that the content of myrosinaldehyde in white Sechium edule was high, followed by coniferol. The lysophosphatidyl ethanolamine was the highest differential metabolite in Jiangtun cyan Sechium edule, followed by lysophosphatidylcholine. The result of KEGG shows that differential metabolites enriched in 5 major metabolic pathways, including purine, linoleic acid, flavonoid biosynthesis, synthesis of secondary metabolites and α-linolenic acid metabolism.[Conclusion] The lipid, flavonoid, alkaloids and nucleotides substances in the tender tips of Jiangtun cyan Sechium edule are high, especially lysophosphatidyl ethanolamine, daidzein, adenosine triphosphate and ferulic spermidine etc. The research results elucidated the nutritional and medicinal basis of the metabolomics of Jiangtun cyan Sechium edule, and provided a theoretical basis for the selection and breeding of high-quality varieties of Chaylte Vine. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
22. 基于非靶向代谢组学技术的氨基脲胁迫雌性栉孔扇贝的毒性作用机制研究.
- Author
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田秀慧, 赵军强, 孙春晓, 徐英江, 郭治诚, 高继庆, 胡顺鑫, and 李焕军
- Abstract
Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
23. Regulatory mechanisms and metabolic changes of miRNA during leaf color change in the bud mutation branches of Acer pictum subsp. mono.
- Author
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Baoli Lin, He Ma, Kezhong Zhang, and Jinteng Cui
- Subjects
LEAF color ,LIQUID chromatography-mass spectrometry ,NON-coding RNA ,MICRORNA ,ANTHOCYANINS ,CAROTENOIDS ,MAPLE ,GENE expression - Abstract
Acer pictum subsp. mono is a colorful tree species with considerable ornamental and economic value. However, little is known about the metabolism and regulatory mechanism of leaf color change in A. p. subsp. mono. To reveal the molecular mechanism of leaf color change in A. p. subsp. mono, the present study examined the bud mutation branches and compared the metabolites of the red leaves (AR) of the bud mutation branches of A. p. subsp. mono with those of the green leaves (AG) of the wild-type branches. It was found that the chlorophyll and carotenoids content of the red leaves decreased significantly, while anthocyanins, and various antioxidant enzymes increased significantly compared with the green leaves. The glycosides cyanidin, pelargonidin, malvidin, petunidin, delphinidin, and peonidin were detected in AR by liquid chromatography-mass spectrometry. The cyanidin glycosides increased, and cyanidin 3-O-glycoside was significantly upregulated. We analyzed the transcriptome and small RNA of A. p. subsp. mono leaves and detected 4061 differentially expressed mRNAs and 116 differentially expressed miRNAs. Through miRNA-mRNA association analysis, five differentially expressed modules were found; one miRNA targeted three genes, and four miRNAs targeted a single gene. Among them, miR160b, miR6300, and miR396g were found to be the key miRNAs regulating stable anthocyanin accumulation in A. p. subsp. mono leaves. By revealing the physiological response of leaf color change and the molecular regulatory mechanism of the miRNA, this study provides new insight into the molecular regulatory mechanism of leaf color change, thereby offering a foundation for future studies. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
24. 烤烟上部叶成熟过程中代谢组的差异分析.
- Author
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贾中林, 郑庆霞, 戴华鑫, 张艳玲, 周会娜, 张仕祥, 周汉平, and 梁太波
- Abstract
Copyright of Tobacco Science & Technology is the property of Tobacco Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
25. 基于GC-TOF-MS的大黄鱼鱼卵鱼露快速发酵 过程中代谢产物分析.
- Author
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周 静, 张华丹, 火玉明, 雷彩玲, 杜希萍, 梁 鹏, 程文健, and 陈丽娇
- Subjects
LARIMICHTHYS ,KREBS cycle ,ORGANIC acids ,UNSATURATED fatty acids ,PHOSPHATE metabolism ,LEUCINE ,THREONINE - Abstract
Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
- Full Text
- View/download PDF
26. Regulatory mechanisms of the green alga Ulva lactuca oligosaccharide via the metabolomics and gut microbiome in diabetic mice
- Author
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Yihan Chen, Weihao Wu, Xiaoyu Ni, Mohamed A. Farag, Esra Capanoglu, and Chao Zhao
- Subjects
Type 2 diabetes ,Ulva lactuca oligosaccharide ,Senescence ,Differential metabolite ,Gut microbiota ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
Type 2 diabetes (T2D) has emerged as one of the most acute public health diseases of the present time, which increases with the population ageing. This study aimed to evaluate the hypoglycaemic activity of Ulva lactuca oligosaccharide (ULO) under ageing-related diabetes conditions in an animal model. The results demonstrated that ULO can promote hypoglycaemia and delay senescence as mediated via GLP-1/GLP-1R pathway to mobilize the intercommunication between the brain and gut. In addition, twenty-six different metabolites and eight different bacteria were screened in the brain and the gut, respectively. A network relationship displayed that all-trans-retinoic acid has positive relationships with Bifidobacterium and Streptococcus, suggesting that plays a potential key role in maintaining the hypoglycaemic and anti-ageing activities of ULO. Based on these findings, ULO might be an efficient therapy for restoring blood glucose metabolism and delaying brain senescence in elderly T2D patients.
- Published
- 2022
- Full Text
- View/download PDF
27. Bioelectricity facilitates carbon dioxide fixation by Alcaligenes faecalis ZS-1 in a biocathodic microbial fuel cell (MFC).
- Author
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Li, Xinyi, Jia, Tianbo, Zhu, Haiguang, Cai, Luhan, Lu, Yubiao, Wang, Jianxin, Tao, Hengcong, and Li, Peng
- Subjects
- *
CARBON dioxide fixation , *MICROBIAL fuel cells , *VITAMIN B2 , *CARBON sequestration , *RENEWABLE energy sources , *CARBON dioxide - Abstract
[Display omitted] • A. faecalis ZS-1 possesses simultaneous bioelectricity generation and CO 2 fixation. • Bioelectricity promotes the formation of electrotrophic bacterial biofilms. • Riboflavin is responsible to inward EET at biocathode during CO 2 -fixing process. • The high expression of ppc gene increases the performance of CO 2 fixation. • Bioelectricity contributes to converting carbon dioxide into lipids in MFC system. The CO 2 fixation mechanism by Alcaligenes faecalis ZS-1 in a biocathode microbial fuel cell (MFC) was investigated. The closed-circuit MFC (CM) exhibited a significantly higher CO 2 fixation rate (10.7%) compared to the open-circuit MFC (OC) (2.0%), indicating that bioelectricity enhances CO 2 capture efficiency. During the inward extracellular electron transfer (EET) process, riboflavin concentration increased in the supernatant while cytochrome levels decreased. Genome sequencing revealed diverse metabolic pathways for CO 2 fixation in strain ZS-1, with potential dominance of rTCA and C4 pathways under electrotrophic conditions as evidenced by significant upregulation of the ppc gene. Differential metabolite analysis using LC-MS demonstrated that CM promoted upregulation of various lipid metabolites. These findings collectively highlight that ZS-1 simultaneously generated electricity and fixed CO 2 and that the ppc associated with bioelectricity played a critical role in CO 2 capture. In conclusion, bioelectricity resulted in a significant enhancement in the efficiency of CO 2 fixation and lipid production. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
28. Effect of Different Accumulative Temperate Zones in Heilongjiang on Glycine Soja Metabolites as Analyzed by Non-Target Metabolomics
- Author
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Guofeng Bao, Liqiang Mu, and Ying Wang
- Subjects
glycine soja ,accumulated temperature zone ,amino acid ,Heilongjiang ,differential metabolite ,Organic chemistry ,QD241-441 - Abstract
To study the effect of growth temperature on the nutritional components and metabolites of the wild soybean (Glycine soja), we analyzed the nutritional components and metabolic gases of the wild soybean in six accumulated temperature regions of the Heilongjiang Province, China, by gas chromatography–time-of-flight mass spectrometry (GC-TOF-MS). A total of 430 metabolites, including organic acids, organic oxides, and lipids, were identified and analyzed using multivariate statistical analysis, orthogonal partial least squares discriminant analysis, principal component analysis, and cluster analysis. Eighty-seven metabolites significantly differed in the sixth accumulated temperature region compared with the other five accumulated temperature regions. The 40 metabolites (such as threonine (Thr) and lysine (Lys)) were found to be elevated in soybeans from the sixth accumulated temperature zone compared with the other five accumulated temperature zones. Through analyzing the metabolic pathways of these metabolites, amino acid metabolism had the greatest influence on wild soybean quality. The results of the amino acid analysis were consistent with those of the GC-TOF-MS and showed that amino acids in wild soybeans from the sixth accumulated temperature zone significantly differed from those of the other zones. Threonine and lysine were the main substances driving these differences. The growth temperature affected the type and concentrations of metabolites in wild soybeans, and the GC-TOF-MS analysis of the effect of growth temperature on wild soybean metabolites was shown to be feasible.
- Published
- 2023
- Full Text
- View/download PDF
29. 基于GC-MS的低盐虾酱低温发酵过程中 代谢组学分析.
- Author
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李文亚, 班雨函, 于宏伟, 马爱进, 桑亚新, and 孙纪录
- Subjects
GAS chromatography/Mass spectrometry (GC-MS) ,MULTIVARIATE analysis ,DISCRIMINANT analysis ,AMINO acids ,HYPOXANTHINE ,ORGANIC acids ,GLUTAMINE - Abstract
Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
- Full Text
- View/download PDF
30. Lipid degradation contributes to flavor formation during air-dried camel jerky processing.
- Author
-
Cao S and Fu Y
- Abstract
Lipids play an important role in flavor formation in meat products. To determine the contribution of lipids to flavor formation during air-dried camel jerky processing, lipid changes were analyzed by UHPLC-Q-Exactive Orbitrap MS/MS in this study, and volatile compounds were identified by HS-SPME-GC-ToF-MS. Results showed that 606 lipid molecules belonging to 30 subclasses were identified and 206 differential lipid molecules were screened out (VIP > 1, P < 0.05); Cer/NS (d18:1/20:0), LPE (18:1), FA (18:0), GlcADG (12:0/24:1), and PE (18:2e/22:5) were identified as potential lipid biomarkers. A total of 96 volatile compounds were also identified, and 16 of these were identified as key aroma compounds in air-dried camel jerky. Meanwhile, 11 differential lipids significantly, negatively correlated with 7 key aroma compounds ( P < 0.05) during processing, indicating that the precursors produced by the degradation of lipid molecules were important sources of volatile flavor substances in air-dried camel jerky., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
31. Emerging application of metabolomics on Chinese herbal medicine for depressive disorder
- Author
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Xinyi Gu, Xingyuan Gao, Jieyi Cheng, Chenyi Xia, Ying Xu, Li Yang, and Mingmei Zhou
- Subjects
Chinese herbal medicine (CHM) ,Depressive disorder ,Metabolomics ,Differential metabolite ,Metabolic pathway ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Depressive disorder is a kind of emotional disorder that is mainly manifested with spontaneous and persistent low mood. Its etiology is complex and still not fully understood. Metabolomics, an important part of system biology characterized by its integrity and systematicness, analyzes endogenous metabolites of small molecules in vivo and examines the metabolic status of the organism. It is widely used in the field of disease research for its unique advantage in the disease molecular marker discovering Due to fewer adverse reactions and high safety, Chinese herbal medicine (CHM) has great advantages in the treatment of chronic diseases including depression. Metabolomics has been gradually applied to the efficacy evaluation of CHM in treatment of depression and the metabolomics analysis exhibits a systemic metabolic shift in amino acids (such as alanine, glutamic acid, valine, etc.), organic acids (lactic acid, citric acid, stearic acid, palmitic acid, etc.), and sugars, amines, etc. These differential metabolites are mainly involved in energy metabolism, amino acid metabolism, lipid metabolism, etc. In this review, we have exemplified the study of CHM in animals or clinics on the depression, and revealed that CHM treatment has significantly changed the metabolic disorders associated with depression, promoting metabolic network reorganization through restoring of key metabolites, and metabolic pathways, which may be the main mechanism basis of CHM’s treatment on depression. Besides, we further envisioned the future application of metabolomics in the study of CHM treatment of depression.
- Published
- 2021
- Full Text
- View/download PDF
32. 基于UHPLC-Q/TOF-MS技术的黄色金针菇菌柄和菌盖成分分析.
- Author
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陈一龙, 李卿, 陈仕江, 张德利, 罗昌树, and 贺宗毅
- Subjects
AMINO acid derivatives ,LIQUID chromatography-mass spectrometry ,ORGANIC acids ,LINOLENIC acids ,SUGAR alcohols ,CITRULLINE ,NICOTINAMIDE ,EXCIMERS - Abstract
Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
- View/download PDF
33. 基于 UHPLC-Q/TOF-MS技术的黄色金针菇菌柄 和菌盖成分分析.
- Author
-
陈一龙, 李即, 陈仕江, 张德利, 罗昌树, and 贺宗毅
- Subjects
AMINO acid derivatives ,LIQUID chromatography-mass spectrometry ,ORGANIC acids ,LINOLENIC acids ,SUGAR alcohols ,CITRULLINE ,NICOTINAMIDE ,EXCIMERS - Abstract
Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
- View/download PDF
34. 基于 UPLC-QTOF-MS 代谢组学研究灰树花发酵的代谢差异.
- Author
-
雷露, 吴天祥, and 王川南
- Subjects
TIME-of-flight mass spectrometry ,CULTURE media (Biology) ,FATTY acids ,AMINO acids ,DISCRIMINANT analysis ,ORGANIC acids ,POLYSACCHARIDES - Abstract
Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
- Full Text
- View/download PDF
35. METABOLOMICS ANALYSIS OF ANABASIS APHYLLA SEEDLINGS UNDER COLD STRESS.
- Author
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CHU, G. M., WANG, T. T., CHANG, Y. L., PENG, M. W., and WANG, M.
- Subjects
AMINOBUTYRIC acid ,KETOGLUTARIC acids ,METABOLOMICS ,PLANT metabolism ,AMINO acids - Abstract
Cold severely affects plant growth and metabolism. The analysis of the characteristics of metabolite variations in seedlings under cold stress can help understand the physiological mechanism of Anabasis aphylla when responding to stress. In this study, gas chromatography-mass spectrometry technology was used to systematically analyze the changing characteristics of the metabolite profiles of A. aphylla under cold stress. A total of 116 types of differential metabolites were stably detected, of which 41 were up-regulated, while 75 were down-regulated. The metabolites of carbohydrates, amino acids, and organic acids formed the majority. The results showed that cold stress had a significant impact on the differential metabolites of A. aphylla seedlings. Via enrichment of metabolites such as γ- aminobutyric acid, α- ketoglutaric acid, and 1-kestose, A. aphylla could reduce the extent of damage caused by cold stress. The results of this study provide a basis for further analysis of the molecular and physiological mechanisms of the cold stress response of A. aphylla. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
36. 基于GC-TOF-MS代谢组学研究高度黑糯米酒后发酵阶段代谢差异.
- Author
-
姜 丽, 苏 伟, 母应春, 王洪琳, and 赵 驰
- Subjects
ORGANIC acids ,STARCH metabolism ,AMINO acids ,METHIONINE metabolism ,PROLINE metabolism ,LEUCINE ,SUCROSE - Abstract
Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
- Full Text
- View/download PDF
37. 基于核磁共振代谢组学分析不同品种草莓的化学成分差异.
- Author
-
安 莉, 汪 红, 马婧玮, 秦冬梅, 朱春雨, 袁永亮, and 吴绪金
- Abstract
Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
38. Steam radish sprout (Raphanus sativus L.): active substances, antioxidant activities and non‐targeted metabolomics analysis.
- Author
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Li, Ru, Hao, Rui, and Zhu, Yi
- Subjects
- *
RADISHES , *STEAM , *SPROUTS , *SUPEROXIDE dismutase , *VITAMIN B2 , *PHENOLIC acids , *FLAVONOIDS , *PLANT phenols - Abstract
Summary: Various active substances, antioxidant activities and primary differential metabolite profiles were investigated to assess the nutritional quality of radish sprouts steamed for 0–300 s. The levels of flavonoids, total phenolic content and isothiocyanate in radish sprouts decreased with increased steaming time, and DPPH radical scavenging activity levels and superoxide dismutase levels also exhibited a downward trend (P < 0.05). By contrast, total phenolic acid, ferric‐reducing antioxidant power and reducing power levels exhibited significant increases in the early short‐term steaming process (P < 0.05). Steaming for 30 s promoted the accumulation of vitamin B2, whereas, steaming for 300 s promoted the accumulation of phospholipids and myristic acid to a greater extent than steaming for 30 s. Overall, there was a partial non‐decreased nutritional indicator in short‐term steaming of radish sprouts, and a longer steaming time positively affected the differential metabolites. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
39. Changes of anthocyanin and amino acid metabolites in saffron petals (Crocus sativus L.) during fermentation based on untargeted metabolomics.
- Author
-
Wang, Liling, Qin, Yuchuan, Wang, Yanbin, and Zhou, Yifeng
- Subjects
- *
ANTHOCYANINS , *SAFFRON crocus , *AMINO acids , *AMINO acid metabolism , *LIQUID chromatography-mass spectrometry , *METABOLOMICS - Abstract
In order to investigate the possibility of fermentation for deep processing of saffron petals, the metabolic profiles of anthocyanins and amino acids in saffron petals were studied by untargeted metabolomics method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS) combined with multivariate statistical analysis. The PCA, OPLS-DA and HCA models were established to analyze the differential metabolites before and after fermentation. Subsequently, the differential metabolites were screened by OPLS-DA with pairwise modeling. The contents of 26 kinds of anthocyanins during fermentation and 23 kinds of anthocyanins after fermentation were significantly different, respectively. After fermentation, the total anthocyanin content increased, and the monomer anthocyanin produced a variety of glucoside anthocyanin. As for amino acid metabolism, there were significant differences in 24 amino acids during fermentation, 27 kinds of amino acids after fermentation, and then the total content of amino acids decreased. In addition, based on the KEEG database, several metabolic pathways during the fermentation process were identified, and the metabolism pathway of anthocyanin is primarily related to anthocyanin biosynthesis, while the metabolic pathway of amino acid is mainly related to metabolic pathways. These findings provide comprehensive insights into the quality improvements of saffron petals wine. • Saffron petals were used for fermentation for the first time. • The types and abundances of amino acid and anthocyanin changed during fermentation. • The PCA, OPLS-DA and HCA models were established to analyze the differential metabolites before and after fermentation. • 26 and 23 kinds of anthocyanins markers were found during and after fermentation, respectively. • 24 and 27 kinds of amino acids during and after fermentation were significant differences, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
40. Differences in body wall metabolites of Apostichopus japonicus of different ages in liquid chromatography-tandem mass spectrometry.
- Author
-
Yang, Xinyu, Han, Lingshu, Zhang, Jinyuan, Wang, Wenpei, Tian, Wanrong, Wang, Yongjie, Chang, Yaqing, Wang, Luo, Hao, Zhenlin, Yin, Donghong, and Ding, Jun
- Subjects
APOSTICHOPUS japonicus ,GERMPLASM conservation ,SULFUR metabolism ,LIQUID chromatography-mass spectrometry ,LIPOIC acid ,TANDEM mass spectrometry ,STEARIC acid - Abstract
Apostichopus japonicus is a marine invertebrate with high economic value. Analysis of the effects of age on the structure of this species is important for understanding the status of the population and conservation. In this study, metabolite analysis of the body walls of A. japonicus of three different ages (1, 2, and 3 years) was performed using LC-MS/MS. Metabolite analysis was performed for the KEGG metabolic pathway. The partial least squares-discriminant analysis is clearly distinguished metabolites in the three groups of A. japonicus. A total of 59, 222, and 179 different metabolites were detected in the age 3 vs age 2, age 3 vs age 1, and age 2 vs age 1 controls, respectively (P < 0.05). These metabolic differences involved several metabolic pathways, including phenylalanine metabolism, taurine and hypotaurine metabolism, sulfur metabolism et al. Thiamin, riboflavin, lipoic acid, acetyl l -carnitine levels increase with age, trans-10-heptadecenoic acid, stearic acid, pentadecanoic acid, dibutyl sebacate, and 8,15-diHETE levels decrease with age, which these metabolites can be used as potential markers to determine the age of A. japonicus. Our results provide the foundation for determining the age of A. japonicus and are important for the conservation of germplasm resources of A. japonicus. [Display omitted] • Lipids molecules, and organic heterocyclic compounds were potential biomarkers for the age identification of A. japonicus. • Thiamine, riboflavin, and lipoic acid, etc. increase with the age of A. japonicus. • Trans 10 heptadecaenoic acid, stearic acid, pentadecanoic acid, etc. decrease with the age of A. japonicus. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
41. Comprehensive investigation on non-volatile and volatile metabolites in four types of green teas obtained from the same tea cultivar of Longjing 43 (Camellia sinensis var. sinensis) using the widely targeted metabolomics.
- Author
-
Shi, Yali, Zhu, Yin, Ma, Wanjun, Shi, Jiang, Peng, Qunhua, Lin, Zhi, and Lv, Haipeng
- Subjects
- *
GREEN tea , *TEA , *OLIVE oil , *METABOLOMICS , *METABOLITES , *GREEN products - Abstract
[Display omitted] • Widely target metabolomics was used to identify the flavour profiles of green teas. • The same cultivar was used to compare the metabolites of four types of green teas. • Differences in metabolites of four types of green tea products were revealed. • Non-volatiles and volatiles changed notably in different manufacturing processes. In this study, we produced roasted, baked, steamed, and sun-dried green tea products using the same batch of fresh tea leaves (FTL) of Longjing 43 (Camellia sinensis var. sinensis), and explored processing effects on the metabolic profiles of four types of green teas (FGTs) using the widely targeted metabolomics. Results showed that 146 differential metabolites including flavonoids, amino acids, lipids, and phenolic acids were screened among 1034 non-volatiles. In addition, nineteen differential metabolites were screened among 79 volatiles. Most of non-volatiles and volatiles metabolites changed notably in different manufacturing processes, whereas there were no significant differences (p >0.05) in the levels of total catechins between FGTs and FTL. The transformation of metabolites was the dominant trend during green tea processing. The results contribute to a better understanding of how the manufacturing process influences green tea quality, and provide useful information for the enrichment of tea biochemistry theory. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
42. Emerging application of metabolomics on Chinese herbal medicine for depressive disorder
- Author
-
Ying Xu, Chenyi Xia, Li Yang, Xingyuan Gao, Xinyi Gu, Jieyi Cheng, and Mingmei Zhou
- Subjects
Metabolic network ,RM1-950 ,Disease ,Pharmacology ,Palmitic acid ,chemistry.chemical_compound ,Metabolomics ,Chinese herbal medicine (CHM) ,Valine ,Medicine ,Animals ,Humans ,Medicine, Chinese Traditional ,chemistry.chemical_classification ,Depressive Disorder ,Differential metabolite ,business.industry ,Lipid metabolism ,General Medicine ,Amino acid ,Metabolic pathway ,chemistry ,Therapeutics. Pharmacology ,business ,Drugs, Chinese Herbal - Abstract
Depressive disorder is a kind of emotional disorder that is mainly manifested with spontaneous and persistent low mood. Its etiology is complex and still not fully understood. Metabolomics, an important part of system biology characterized by its integrity and systematicness, analyzes endogenous metabolites of small molecules in vivo and examines the metabolic status of the organism. It is widely used in the field of disease research for its unique advantage in the disease molecular marker discovering Due to fewer adverse reactions and high safety, Chinese herbal medicine (CHM) has great advantages in the treatment of chronic diseases including depression. Metabolomics has been gradually applied to the efficacy evaluation of CHM in treatment of depression and the metabolomics analysis exhibits a systemic metabolic shift in amino acids (such as alanine, glutamic acid, valine, etc.), organic acids (lactic acid, citric acid, stearic acid, palmitic acid, etc.), and sugars, amines, etc. These differential metabolites are mainly involved in energy metabolism, amino acid metabolism, lipid metabolism, etc. In this review, we have exemplified the study of CHM in animals or clinics on the depression, and revealed that CHM treatment has significantly changed the metabolic disorders associated with depression, promoting metabolic network reorganization through restoring of key metabolites, and metabolic pathways, which may be the main mechanism basis of CHM’s treatment on depression. Besides, we further envisioned the future application of metabolomics in the study of CHM treatment of depression.
- Published
- 2021
43. Regulatory mechanisms and metabolic changes of miRNA during leaf color change in the bud mutation branches of Acer pictum subsp . mono .
- Author
-
Lin B, Ma H, Zhang K, and Cui J
- Abstract
Acer pictum subsp. mono is a colorful tree species with considerable ornamental and economic value. However, little is known about the metabolism and regulatory mechanism of leaf color change in A. p. subsp. mono . To reveal the molecular mechanism of leaf color change in A. p. subsp. mono , the present study examined the bud mutation branches and compared the metabolites of the red leaves (AR) of the bud mutation branches of A. p. subsp. mono with those of the green leaves (AG) of the wild-type branches. It was found that the chlorophyll and carotenoids content of the red leaves decreased significantly, while anthocyanins, and various antioxidant enzymes increased significantly compared with the green leaves. The glycosides cyanidin, pelargonidin, malvidin, petunidin, delphinidin, and peonidin were detected in AR by liquid chromatography-mass spectrometry. The cyanidin glycosides increased, and cyanidin 3-O-glycoside was significantly upregulated. We analyzed the transcriptome and small RNA of A. p. subsp. mono leaves and detected 4061 differentially expressed mRNAs and 116 differentially expressed miRNAs. Through miRNA-mRNA association analysis, five differentially expressed modules were found; one miRNA targeted three genes, and four miRNAs targeted a single gene. Among them, miR160b, miR6300, and miR396g were found to be the key miRNAs regulating stable anthocyanin accumulation in A. p. subsp. mono leaves. By revealing the physiological response of leaf color change and the molecular regulatory mechanism of the miRNA, this study provides new insight into the molecular regulatory mechanism of leaf color change, thereby offering a foundation for future studies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Lin, Ma, Zhang and Cui.)
- Published
- 2023
- Full Text
- View/download PDF
44. Regulatory mechanisms of the green alga Ulva lactuca oligosaccharide via the metabolomics and gut microbiome in diabetic mice.
- Author
-
Chen Y, Wu W, Ni X, Farag MA, Capanoglu E, and Zhao C
- Abstract
Type 2 diabetes (T2D) has emerged as one of the most acute public health diseases of the present time, which increases with the population ageing. This study aimed to evaluate the hypoglycaemic activity of Ulva lactuca oligosaccharide (ULO) under ageing-related diabetes conditions in an animal model. The results demonstrated that ULO can promote hypoglycaemia and delay senescence as mediated via GLP-1/GLP-1R pathway to mobilize the intercommunication between the brain and gut. In addition, twenty-six different metabolites and eight different bacteria were screened in the brain and the gut, respectively. A network relationship displayed that all-trans-retinoic acid has positive relationships with Bifidobacterium and Streptococcus , suggesting that plays a potential key role in maintaining the hypoglycaemic and anti-ageing activities of ULO. Based on these findings, ULO might be an efficient therapy for restoring blood glucose metabolism and delaying brain senescence in elderly T2D patients., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
45. Integration of non-targeted metabolomics and E-tongue evaluation reveals the chemical variation and taste characteristics of five typical dark teas.
- Author
-
Cheng, Lizeng, Wang, Yuanfeng, Zhang, Jiarong, Xu, Lurong, Zhou, Hui, Wei, Kang, Peng, Lanlan, Zhang, Jie, Liu, Zhonghua, and Wei, Xinlin
- Subjects
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TEA growing , *BITTERNESS (Taste) , *LIQUID chromatography-mass spectrometry , *TEA , *FLAVONOID glycosides , *ELECTRONIC tongues , *METABOLOMICS - Abstract
The chemical profile and taste quality of various dark teas vary considerably due to the huge differences in tea cultivar, processing technology, and production region. Herein, we integrated liquid chromatography-mass spectrometry and electronic tongue to reveal variations in chemical profile and taste quality of five typical dark teas, namely Pu-erh tea (PET), Fuzhuan tea (FZT), Qingzhuan tea (QZT), Kangzhuan tea (KZT), and Liubao tea (LBT), and elucidate chemical basis for their taste quality. The chemical phenotypes of dark teas were classified into FZT, PET, and QZT-KZT-LBT, whereas their taste phenotypes were classified into FZT, PET-QZT, and KZT-LBT. Totally, 49 compounds were identified as differential metabolites, with higher contents of catechins and flavonoid glycosides in FZT, and higher contents of catechin derivatives, flavonoids, and alkaloids in PET. PET had the strongest bitterness and aftertaste-bitterness, while FZT showed the strongest astringency, aftertaste-astringency, and saltiness among five typical dark teas. The bitterness and aftertaste-bitterness of dark teas were negatively associated with polyphenols, flavonoids, and polysaccharides, but positively associated with theabrownins; their aftertaste-astringency was positively related to polyphenols and flavonoids, but negatively related to theabrownins. This study will guide dark tea taste quality control and lay a foundation for comparing their health benefits. • A total of 49 compounds were identified as differential metabolites. • FZT had higher contents of catechins and flavonoid glycosides compared with others. • The taste phenotypes of dark teas were classified into FZT, PET-QZT, and KZT-LBT. • PET had the strongest bitterness and aftertaste-bitterness in five dark teas. • The aftertaste-astringency of dark teas was negatively related to theabrownins. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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46. Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
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Liu, Zhengqi, Zhang, Yujiao, Ai, Chunqing, Wen, Chengrong, Dong, Xiuping, Sun, Xiaona, Cao, Cui, Zhang, Xueqian, Zhu, Beiwei, and Song, Shuang
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GUT microbiome , *SEA cucumbers , *APOSTICHOPUS japonicus , *FERMENTATION , *FUNCTIONAL assessment , *FATTY acids , *POLYSACCHARIDES - Abstract
[Display omitted] • Microbial consortiums from different individuals response discrepantly to SCSP sj. • Parabacteroides distasonis plays a critical role in SCSP sj utilization. • More beneficial metabolites were accumulated by microbiota consuming more SCSP sj. Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSP sj) has been inferred to benefit the host health via modulating gut microbiota composition. The present study compared the responses of gut microbiota communities from different donors to SCSP sj , and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria. Gut microbiota communities from 6 donors (A ~ F) utilized the polysaccharides to different degrees in vitro fermentation. Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSP sj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides. Further in vitro fermentation of SCSP sj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis , enriched in Sample C, plays a critical role in the utilization of the polysaccharides. Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides. Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis , are critical for SCSP sj utilization in gut so as to influence the benefits of the polysaccharide supplement for host. Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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47. Emerging application of metabolomics on Chinese herbal medicine for depressive disorder.
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Gu, Xinyi, Gao, Xingyuan, Cheng, Jieyi, Xia, Chenyi, Xu, Ying, Yang, Li, and Zhou, Mingmei
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MENTAL depression , *HERBAL medicine , *CHINESE medicine , *METABOLOMICS , *AMINO acid metabolism - Abstract
Depressive disorder is a kind of emotional disorder that is mainly manifested with spontaneous and persistent low mood. Its etiology is complex and still not fully understood. Metabolomics, an important part of system biology characterized by its integrity and systematicness, analyzes endogenous metabolites of small molecules in vivo and examines the metabolic status of the organism. It is widely used in the field of disease research for its unique advantage in the disease molecular marker discovering Due to fewer adverse reactions and high safety, Chinese herbal medicine (CHM) has great advantages in the treatment of chronic diseases including depression. Metabolomics has been gradually applied to the efficacy evaluation of CHM in treatment of depression and the metabolomics analysis exhibits a systemic metabolic shift in amino acids (such as alanine, glutamic acid, valine, etc.), organic acids (lactic acid, citric acid, stearic acid, palmitic acid, etc.), and sugars, amines, etc. These differential metabolites are mainly involved in energy metabolism, amino acid metabolism, lipid metabolism, etc. In this review, we have exemplified the study of CHM in animals or clinics on the depression, and revealed that CHM treatment has significantly changed the metabolic disorders associated with depression, promoting metabolic network reorganization through restoring of key metabolites, and metabolic pathways, which may be the main mechanism basis of CHM's treatment on depression. Besides, we further envisioned the future application of metabolomics in the study of CHM treatment of depression. • CHM has great advantages in the treatment of depressive disorder. • Metabolomics is widely used in the field of CHM. • Metabolomics reveals the mechanism of CHM in the treatment of depression. [ABSTRACT FROM AUTHOR]
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- 2021
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48. Metabolomics responses of Bambusa pervariabilis × Dendrocalamopsis grandis varieties to Biotic (pathogenic fungus) stress.
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Li, Shujiang, He, Qianqian, Peng, Qi, Fang, Xinmei, Zhu, Tianhui, Qiao, Tianmin, and Han, Shan
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METABOLOMICS , *PATHOGENIC fungi , *CATIONS , *ANIONS , *GLUTAMINE , *PROLINE metabolism - Abstract
Bambusa pervariabilis × Dendrocalamopsis grandis blight, caused by Arthrinium phaeospermum , is one of the most common and serious diseases in bamboo and occurs in the newly born twigs. Bamboo has suffered large dead areas, including more than 3000 hm2, which greatly threatens the process of returning farmlands to forests and the construction of ecological barriers. To identify differential metabolites and metabolic pathways associated with B. pervariabilis × D. grandis to A. phaeospermum , ultra-performance liquid chromatography (UPLC) and quadrupole-time of flight (Q-TOF) Mass Spectrometry (MS) combined with a data-dependent acquisition method was used to analyse the entire sample spectrum. In total, 13223 positive ion peaks and 10616 negative ion peaks were extracted. OPLS-DA and several other analyses were performed using the original data. The OPLS-DA models showed good quality and had strong predictive power, indicating clear trends in the analyses of the treatment and control groups. Clustering and KEGG pathway analyses were used to screen the differential metabolites in the treatment and control groups from the three B. pervariabilis × D. grandis varieties and reflected their metabolic responses induced by A. phaeospermum infection. The results showed that the three B. pervariabilis × D. grandis varieties mode showed significant changes in the following six resistance-related metabolites after A. phaeospermum invasion in positive and negative ion modes: proline, glutamine, dictamnine, apigenin 7-O-neohesperidoside, glutamate, and cis-Aconitate. The following four main metabolic pathways are involved: Arginine and proline metabolism, Glyoxylate and dicarboxylate metabolism, Biosynthesis of alkaloids derived from shikimate pathway, and Flavone and flavonol biosynthesis. This study lays a foundation for the later detection of differential metabolites and metabolic pathways for targeting, and provides a theoretical basis for disease-resistant breeding and the control of B. pervariabilis × D. grandis blight. Image 1 • The application methods of UPLC-Q-TOF MS in plant metabolomics are reviewed. • Non-targeted metabolomics can characterize all metabolites as much as possible. • Differential metabolomics are associated with the fungal stress in bamboo varieties. • Six metabolites and four metabolic pathways in resistance have significant changes. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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