1. Apoptotic Effects of Anthocyanins from
- Author
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Cheol, Park, Won Sup, Lee, Se-Il, Go, Sang-Ho, Jeong, Jiyun, Yoo, Hee-Jae, Cha, Young-Joon, Lee, Heui-Soo, Kim, Sun-Hee, Leem, Hye Jung, Kim, Gon Sup, Kim, Soon-Chan, Hong, and Yung Hyun, Choi
- Subjects
Membrane Potential, Mitochondrial ,anticancer effects ,MKN28 human gastric carcinoma cells ,apoptosis ,Cell Cycle Proteins ,X-Linked Inhibitor of Apoptosis Protein ,Vitis coignetiae Pulliat ,anthocyanins ,Article ,enhancer of the rudimentary homolog ,Enzyme Activation ,Proto-Oncogene Proteins c-bcl-2 ,Stomach Neoplasms ,Caspases ,Cell Line, Tumor ,Humans ,Vitis ,Reactive Oxygen Species ,Cell Proliferation ,Transcription Factors - Abstract
Evidence suggests that augmented expression of a certain gene can influence the efficacy of targeted and conventional chemotherapies. Here, we tested whether the high expression of enhancer of the rudimentary homolog (ERH), which serves as a prognostic factor in some cancers, can influence the efficacy of anthocyanins isolated from fruits of Vitis coignetiae Pulliat, Meoru in Korea (AIMs) on human gastric cancer cells. The anticancer efficacy of AIMs was augmented in ERH-transfected MKN28 cells (E-MKN28 cells). Molecularly, ERH augmented AIM-induced caspase-dependent apoptosis by activating caspase-3 and -9. The ERH-augmented apoptotic effect was related to mitochondrial depolarization and inhibition of antiapoptotic proteins, XIAP, and Bcl-2. In addition, reactive oxygen species (ROS) generation was augmented in AIMs-treated E-MKN28 cells compared to AIMs-treated naïve MKN28 cells. In conclusion, ERH augmented AIM-induced caspase-dependent mitochondrial-related apoptosis in MKN28 cells. A decrease in expression of Bcl-2 and subsequent excessive ROS generation would be the mechanism for ERH-augmented mitochondrial-related apoptosis in AIMs-treated MKN28 cells. A decrease in expression of XIAP would be another mechanism for ERH-augmented caspase-dependent apoptosis in AIMs-treated MKN28 cells.
- Published
- 2021