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Fish bacterial infectious diseases are a signifi cant problem for aquaculture, often leading to massive fi sh deaths and signifi cant economic losses. For fi sh treatment often used antibacterial drugs: nitrofuran, antibiotics, feed antibiotics, as well as dyes. Finding eff ective drugs and studying their eff ects on the body of fi sh are relevant. Nowadays, diff erent groups of substances, including probiotics, are off ered as an alternative to increase the growth of animals and to combat pathogens. Here are the results of studies of the infl uence of the probiotic drug "Enteronormin" on the indicators that characterize the status of T- and B-cell immunity of carp aff ected by bacterial Aeromonosis. Enteronormin is a probiotic supplement , wich contains a bacteria complex - Enterococcus spp, Bacillus subtilis spp, Lactobacillus spp; and excipients - chitosan, peptone enzymatic and ground sugar (РП № ВВ-00427-02-12 від 13.04.2012). The research was conducted under the conditions of aquariums at the Lviv Research Station of the Institute of Fisheries of NAAS. The control group, which consisted of clinically healthy fi sh, received only 3% starch suspension, the fi rst experimental group (D1) - Aeromonosis aff ected carps who were given only 3% starch suspension, the second experimental group (D2), which consisted of carps aff ected Aeromonosis, through the probe within 7 days was administered the drug "Enteronormin", at the rate of 2 mg per 1 kg of fi sh weight, in the composition of 3% starch suspension. Before feeding for the carp drug was activated for 14-16 hours water enriched with Iodine and Selenium ions in the Jodis + Se form (TU U 15.7-30631018-011: 2011). It is established that the disease of carp on Aeromonosis leads to changes in the number and functional activity of immunocompetent blood cells. The inhibition of lymphocytogenesis and functional activity of immunocompetent cells in carp blood in a disease associated with the bacterial form of aeromonosis was ascertained. The use in the experimental carps group in the composition of 3% starch suspension of the drug "Enteronormin" had a normalizing eff ect on the state of T-cell specifi c protection, but did not signifi cantly aff ect the amount of EAC-RUL and their functional activity.

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The objective of this study is to identify fi sh protein markers for detecting multiple species based on a comparative proteomic approach that relies on fragments with identical sequences. The possibilities and challenges of the use of peptides obtained from carp (Cyprinus carpio) and herring (Clupea harengus) proteins are discussed. A bioinformatic analysis was followed by an LC-MS/MS experiment to identify markers predicting the presence of fi sh allergenic proteins. Selected myosin peptides were found in carp protein hydrolysates with known sequences and in herring protein hydrolysates with unknown sequences. The results obtained for carp and herring proteins myosin and parvalbumin indicate that proteins with unknown sequences can be identified by peptide markers. Such markers can be designed by disregarding the principle that peptides should be unique (present in one sequence). The challenge is to determine a group of proteins that can be detected by peptide identification. [ABSTRACT FROM AUTHOR]

Copyright of Collegium Antropologicum is the property of Croatian Anthropological Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)