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Food contamination of pathogenic bacteria poses a serious threat to human health. Accurate and rapid detections of pathogenic bacteria in food stuff are of great concerns， which are necessary to reduce outbreaks of foodborne diseases. Although traditional culture­based detection of pathogen are reliable and classical， they suffer from cumbersome and time­consuming. Recently， various detection strategies based on electrochemistry and optical spectroscopy using advanced nanomaterials and micro­analytical systems have emerged as portable and ultrasensitive sensing platform. This review focuses on several typical rapid detection method for foodborne pathogens， and emphasizes the negative effect of interference to these detection systems. Some sample processing method for achieving accurate detection are listed. Finally， taking typical food­borne pathogens as examples， the development status and future research directions of rapid detection technology are briefly described.

• A Boronate Affinity Material for bacterial enrichment was prepared. • The influence of PEIs, ligand density, pH and ion strength were evaluated. • The material was successfully applied to the enrichment of pathogenic bacteria. Detection of pathogenic bacteria by polymerase chain reaction (PCR) is progressively emerging, although it is still hindered by a complex matrix, long-term bacterial enrichment and low bacterial abundance. Here, we report a novel material based on boronate affinity for recognition and enrichment of bacteria using a pre-PCR method. After optimization, the material exhibited high boronate affinity toward bacteria, with adsorption capacities of S. aureus and Salmonella spp. incubated in 0.01 M PBS (pH 7.4) at 37 °C for 15 min calculated as (906.60 ± 15.73) × 107 cfu/g and (582.59 ± 13.19) × 107 cfu/g, respectively, without any bacterial death during the binding process. The material was then applied to enrich S. aureus and Salmonella spp. from the spiked water and 25% cow milk samples followed by mPCR, which resulted in high bacterial enrichment and demonstrated great potential for selective enrichment of bacteria in food samples. [ABSTRACT FROM AUTHOR]