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The present study assessed the health and growth of goat kids raised in stall-fed and free-range grazing systems during summer and winter. Parameters measured included Temperature Humidity Index (THI), weight gain, rectal temperature, hemoglobin (Hb), total leukocyte count (TLC), packed cell volume (PCV), antioxidant enzymes (SOD, GSH), total proteins, glucose, triglycerides, and cholesterol. Stall-fed kids gained significantly more weight (P<0.05) than free-range kids in both seasons. Although rectal temperature increased in summer, it remained within physiological limits. Hemoglobin levels were significantly higher (P<0.05) in winter, while TLC, Hb, and PCV decreased in summer. Free-range kids had significantly higher antioxidant enzyme levels (SOD, GSH) in both seasons. During summer a significant increase in total proteins and a decrease in glucose, triglycerides, and cholesterol in both groups was reported. Stall-reared kids exhibited better overall growth and health, but antioxidant status was superior in free-range kids. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the effect of different environmental enrichment tools on behavior, welfare, productive performance, and egg quality traits of Naked Neck chickens. A total of 1080 Naked Neck female chickens (30 weeks old) were used in the present study. A completely randomized experimental design, with 3 treatments having 4 replicates of 90 birds each, was applied. Treatments consisted of 3 different range areas A (121.9 m2), B (152.4 m2), and C (190.5 m2). Behavior (walking, feeding/foraging, sitting, aggressiveness wing flapping, perching, dust bathing, and range use), welfare (foot pad dermatitis, lameness, gait score, and feather pecking), productive performance (egg weight, egg production %, egg mass, and feed intake), egg quality traits (morphometry, egg weight, shell, albumen, and yolk characteristics) were evaluated. There was no influence (P > 0.05) of different range area on bird’s behavior, and egg quality traits except walking and rang usage. The range area C promoted productive performance including egg weight, egg production %, egg mass and feed intake, but it did not influence welfare traits. It might be concluded that Naked Neck chicken when reared in free range with different range enrichments did not reveal any difference regarding behavioral parameters, egg morphometry, and egg quality traits. The range area within minimum length and effectively used environmental enrichments (perching and dust bathing area) should be used for backyard poultry to obtain ideal performance. [ABSTRACT FROM AUTHOR]

The aim of the study was to determine the changes occurring in the eggs of helmeted guinea fowl (Numida meleagris) from free-range farming in relation to the laying season and storage time. The experimental material consisted of 360 guinea fowl eggs, collected in the first, second and third laying seasons and stored for 7, 14 and 21 days. After each period, physical and physicochemical characteristics of the eggs were determined, as well as the basic chemical composition and mineral content of the albumen and yolk and the yolk fatty acid profile. The age of the guinea fowls affected certain physical parameters of the eggs. The egg weight, shape index and shell thickness increased with the age of the laying hens; however, a decrease in the proportion of shell in the egg was demonstrated. Storage time had a significant effect on egg weight, weight loss during storage and air cell height. Significant differences were found in the chemical composition of guinea fowl eggs depending on the age of the laying hens. Eggs obtained from older laying hens were characterized by higher yolk fat content and lower ash content, while the albumen contained higher water content and lower ash content. During the three-year laying period, changes were observed in the mineral composition of the eggs. The fatty acid profile underwent significant changes; however, no important differences were observed in the total content of SFA, MUFA, PUFA and n-6 fatty acids. Conversely, significant differences were found for n-3 acids and the n-6/n-3 ratio. Eggs in the first and second laying seasons exhibited the most favorable composition. The slow dynamics of changes occurring in successive laying seasons and egg storage time indicated that the raw material studied was safe and could be used by consumers. [ABSTRACT FROM AUTHOR]

Simple Summary: Simple Summary: Recent studies have delved into the adverse phenomenon of smothering in cage-free laying hen-rearing systems, challenging the traditional notion of this behavior as "natural" or the result of hysteria among birds in the flock. This work identifies smothering as a detrimental, abnormal behavior with significant economic repercussions for poultry farming. Through a comprehensive literature review and bibliographic mapping, combined with consultations with poultry farmers via extension services and rural technical assistance, this study illuminates the environmental triggers of smothering behavior. The investigation reveals that factors inherent to the birds' rearing environment precipitate this behavior, underscoring an urgent need for detailed, focused research into avian behavioral physiology. The objective is to unravel the complex interplay between production systems, animal welfare, and their economic implications on poultry operations. This study not only advances our understanding of bird behavior in intensive production contexts but also offers valuable insights for improving welfare standards and economic outcomes in the poultry industry. The proliferation of rearing systems providing opportunities for birds to engage in natural behaviors can trigger behavioral repertoires that when not manageable compromise animal welfare and the economic viability of the flock. Smothering in laying hens has long been perceived as "natural" or the result of hysteria among birds in the flock. However, the current literature has recognized smothering as an abnormal outcome with the potential to result in significant losses in cage-free poultry systems. Recent studies have specifically aimed to categorize the organization of smothering behavior and highlight its potential causes and consequences. In this study, literature review and bibliographic mapping, drawing on published articles and engagement with poultry farmers through extension and rural technical assistance, were employed. The findings indicate that smothering is a behavior triggered by factors related to the environment in which the laying hens are kept. This study concludes that there is a critical need for more rigorous and detailed research to elucidate the nuances of avian behavioral physiology and assess the impact of production systems on animal welfare and the economic impacts on the flock. This research contributes to a deeper understanding of bird behavior in high-production environments and provides practical insights for the poultry industry. [ABSTRACT FROM AUTHOR]

Summary: The objective of this study was to apply multivariate data analytical methods to evaluate the effect of both storage conditions (cold and room temperature) and production systems (cage and free range) on the physical properties of chicken eggs produced under tropical conditions. The results of this study indicated that most of the physical quality parameters (Haugh units, egg weight, albumin height, yolk colour, shell breaking and shell thickness) measured in the egg samples stored at room temperature (RT) were lower compared with egg samples stored in cold room (CT) conditions. The weight loss rate (WLR) was higher in the egg samples from RT compared with CT conditions. Both egg samples from either CT storage conditions or FR production system had the best quality parameters (high Haugh units, heavy egg weights and low WLR). The highest classification rate using linear discriminant analysis was obtained for the prediction of systems of production where egg samples from cage (C) and FR were 83% and 80% correctly classified. However, the classification of weeks of storage provides with intermediate classification results based on the physical parameters measured where 75% and 72% of the samples were correctly classified as stored in CT or RT storage conditions, respectively. The utilisation of multivariate data analysis allowed for the analysis of all the variables at the time without the need to isolate effects such as birds age, housing systems or by the solely interpretation of a single parameter. [ABSTRACT FROM AUTHOR]

Simple Summary: A ready supply of eggs has been provided for consumers throughout the years at a relatively low price sufficient to meet consumer demand. In response to the cage-free trend, adequate studies regarding the difference between the cage system and alternative systems are necessary due to the growing concerns about food safety and animal welfare, especially animal living conditions. Along with welfare concerns, there is rising consumer consciousness about the environmental sustainability of animal production. From this perspective, increasing persistency in lay and stability in egg quality would generate environmental and economic benefits. The present study investigated the effects of the enriched cage and free-range systems on the egg yolk nutrient composition at the late phase of the laying cycle (from the 68th week to the 74th week of age). The results of the present study indicated that the egg yolk lipid profile in terms of the total lipid, cholesterol, and fatty acid composition was not modified by the increasing age of laying hens. On the other hand, the results confirmed that the free-range system may improve the nutritional yolk fatty acid profile and its positive impact on human health, which can be a convincing support for the trend of "cage-free" eggs. In recent years, the free-range system for laying hens has increased, driven by societal sensitivity to animal welfare. This study aimed to comparatively analyze the total lipid, cholesterol, and fatty acid composition of egg yolks of late-age laying hens reared in enriched cages (C) and the free-range system (FR). Eggs were collected from Lohmann Brown Classic hens at the 68th, 70th, 72nd, and 74th week of age. The concentrations of total lipids and cholesterol were not affected (p > 0.05) by either factor. Egg yolk from the FR group showed lower (p < 0.01) monounsaturated fatty acids and higher (p < 0.01) polyunsaturated fatty acid (PUFA) compared with that of the C group. From a nutritional point of view, the PUFA n-6/n-3 and the PUFA/SFA ratios of egg yolk from the FR group were favorably lower and higher (p < 0.01) compared with the C one. Conversely, hen age did not affect (p > 0.05) the fatty acid composition of yolks. Interactions between factors were found for total n-3 and n-6 PUFA and the n-6/n-3 ratio (p < 0.01), as well as the thrombogenic index (p < 0.05). In conclusion, the results confirmed that the free-range system may improve the nutritional yolk fatty acid profile and its positive impact on human health. [ABSTRACT FROM AUTHOR]

Consumers' interest in animal welfare-friendly systems with outdoor access is growing and therefore the necessity has arisen for genotypes suitable for free-range systems. This study aimed to investigate the suitability of two slow-growing broiler genotypes by comparing growth performance, pasture usage, carcass yield and breast meat traits. Two slow growing genotypes Hubbard ISA Red JA-57 (n= 240) and Sasso XL44 × SA51A (n= 240), were raised in free-range system for 63 days, and their suitability was assessed. Body weight, feed consumption and feed conversion rate were weekly determined. To assess the pasture usage (interior, buffer and outer zones), the observations were performed twice a day. The total of 60 birds (n: 30 broilers/genotype) were randomly sampled for slaughter process at 63rd day of age, and subsequently breast muscle samples were processed for the physical quality and chemical composition parameters of the meat. At 63 days of age, the final body weight was found to be 2918.0 g and 3253.6 g in Hubbard and Sasso birds respectively (P<0.001). Also, a higher body weight gain was observed for Sasso birds than Hubbard birds as well (3210.2 vs. 2874.8 g, P<0.001). The broilers preferred to pasture at the interior zone rather than buffer and outer zones (P<0.001), and usually in the morning (27.54%) than in the evening (20.93%, P=0.010). The average slaughter weight, carcass weight and carcass yield were higher in Sasso genotype (3296.7, 2540.4 g, 77.1%, respectively] at 63 days of age compared to Hubbard genotype (2878.3, 2192.3g and 76.2%, respectively, P<0.001). The weight and relative weight of breast were also higher in Sasso (746.2 g and 29.4% respectively) than the Hubbard genotype (617.6 g and 28.2% respectively, P>0.001). These findings could help free-range broiler producers to choose a more suitable genotype according to the final body weight, feed efficiency, pasture usage, carcass yield, and breast meat characteristics. [ABSTRACT FROM AUTHOR]

The aim of the present study was to evaluate the effects of feed restriction and the combination of feed restriction with pasture on the performance parameters of fast-growing chickens, the proportion of internal organs and the caecum microbiota. In the experiment, one-day-old Ross 308 chickens were divided into three groups. Group 1 was fed ad libitum (AL), group 2 was restricted on feed at a rate of 70% ad libitum (R), group 3 was restricted to the same age and level as group 2, and was restricted to 22 days on pasture (FR). Feed restriction and the combination of feed restriction and pasture significantly reduced body weight beginning at the age of 14 days and at the end of the experiment (at 35 days), mortality, and the European Production Efficiency Factor (EPEF) but improved the feed conversion ratio (FCR) beginning at the 4th week of age. Conversely, feed restriction and the combination of feed restriction and pasture significantly increased the proportion of liver, gizzard and Lactobacillus in the caecum. In summary, feed restriction and the combination of feed restriction in the free range had negative effects on growth, feed conversion ratio and economic profit, presumably because of the short realimentation period and because pasture did not have a beneficial effect. [ABSTRACT FROM AUTHOR]

Determining egg freshness is critical for ensuring food safety and security and as such, different methods have been evaluated and implemented to accurately measure and predict it. In this study, a portable near-infrared (NIR) instrument combined with chemometrics was used to monitor and predict the storage time of eggs under two storage conditions—room temperature (RT) and cold (CT) storage—from two production systems: cage and free-range. A total of 700 egg samples were analyzed, using principal component analysis (PCA) and partial least squares (PLS) regression to analyze the NIR spectra. The PCA score plot did not show any clear separation between egg samples from the two production systems; however, some egg samples were grouped according to storage conditions. The cross-validation statistics for predicting storage time were as follows: for cage and RT eggs, the coefficient of determination in cross validation (R2CV) was 0.67, with a standard error in cross-validation (SECV) of 7.64 days and residual predictive deviation (RPD) of 1.8; for CT cage eggs, R2CV of 0.84, SECV of 5.38 days and RPD of 3.2; for CT free-range eggs, R2CV of 0.83, SECV of 5.52 days and RPD of 3.2; and for RT free-range eggs, R2CV of 0.82, SECV of 5.61 days, and RPD of 3.0. This study demonstrated that NIR spectroscopy can predict storage time non-destructively in intact egg samples. Even though the results of the present study are promising, further research is still needed to further extend these results to other production systems, as well as to explore the potential of this technique to predict other egg quality parameters associated with freshness. [ABSTRACT FROM AUTHOR]

The study was conducted to determine the effect of duration on free range on broiler chickens, in relation to sensory characteristics. Two hundred (200) unsexed Arbor broiler chicks were randomly assigned to four treatments. The birds were transferred to the free-range paddock, at 2, 4 and 6 weeks. Some of the birds were reared indoors throughout the rearing period and these served as the control (week 0). The results indicated that the production system had no effect (p>0.05) on sensory traits for broiler chickens. Sensory results showed that there was no difference in juiciness, tenderness, flavour and aroma between meats from free ranged and conventionally raised broiler chickens. Meat floss from week 0 broiler chickens had higher (p<0.05) sensory ratings for colour, aroma, flavour tenderness and juiciness. Overall acceptability showed that the meat floss from weeks 2, 4 and 6 scored above intermediate (7.2, 7.0, and 6.75, respectively), which indicated that all the products irrespective of the rearing system were acceptable to the panellists. It was evident that meat floss from broiler chickens at week 0 was more preferred by the panellists than the other treatments. [ABSTRACT FROM AUTHOR]

Acquiring land that meets the required standards for lowland ponds is becoming an increasing problem in the city of Kinshasa in the Democratic Republic of Congo, and fish farming in above-ground concrete tanks is emerging as an alternative. The objective of this study was to follow the evolution of pre-growth of Oreochromis niloticus Linnaeus, 1758 in an above-ground concrete tank culture system using feed based on local agricultural by-products available in Kinshasa. A total of 198 fries were distributed in 3 tanks and fed three times a day. The experiments were conducted for 60 days between August and November 2021. The results obtained indicate a better survival rate (T1 = 75.75±4.32%; T2 = 60.6±9.76% and T3 = 83.33±4.96%). Feed T1 (mean weight = 33.15±2.78 g) and T2 (28.44±3.76 g) had a positive impact on fish weight growth. The cost of producing one kilogram of a feed ration varied depending on the ingredients used. It cost 2,843 FC to develop the T1 feed, 951 FC to produce the T2 feed and 5.000 FC to afford the T3 commercial feed sold in Kinshasa. Ration T2 is the one that gave a better compromise of price and quality by promoting good fish growth at a lower cost (1,692 Congolese Francs) than the other two feeds. These observations sufficiently show that during the experiment, the experimental structure (concrete tank) did not have negative effects on the zootechnical performance of the fry of the fish species studied. The economic aspect of the use of feed rations by fry showed the merits of rearing fish using local agricultural by-products rather than commercial feed. This study showed the merits of setting up an above-ground concrete tank fish farm to overcome the difficulty of acquiring land to meet fish farming requirements.

The aim of the study was to determine the changes occurring in the eggs of helmeted guinea fowl (Numida meleagris) from free-range farming in relation to the laying season and storage time. The experimental material consisted of 360 guinea fowl eggs, collected in the first, second and third laying seasons and stored for 7, 14 and 21 days. After each period, physical and physicochemical characteristics of the eggs were determined, as well as the basic chemical composition and mineral content of the albumen and yolk and the yolk fatty acid profile. The age of the guinea fowls affected certain physical parameters of the eggs. The egg weight, shape index and shell thickness increased with the age of the laying hens; however, a decrease in the proportion of shell in the egg was demonstrated. Storage time had a significant effect on egg weight, weight loss during storage and air cell height. Significant differences were found in the chemical composition of guinea fowl eggs depending on the age of the laying hens. Eggs obtained from older laying hens were characterized by higher yolk fat content and lower ash content, while the albumen contained higher water content and lower ash content. During the three-year laying period, changes were observed in the mineral composition of the eggs. The fatty acid profile underwent significant changes; however, no important differences were observed in the total content of SFA, MUFA, PUFA and n-6 fatty acids. Conversely, significant differences were found for n-3 acids and the n-6/n-3 ratio. Eggs in the first and second laying seasons exhibited the most favorable composition. The slow dynamics of changes occurring in successive laying seasons and egg storage time indicated that the raw material studied was safe and could be used by consumers

ABSTRACT: The poultry industry is facing continuous challenges with regard to increased feed costs and loss due to infectious disease. To overcome this challenge, several antibiotics have been used along with chicken feeds to promote growth. Nevertheless, the use of antibiotics as growth promoter has been banned in many countries, due to the concerns associated with potential risks of emerging and horizontal transfer of multidrug resistant genes to bacteria in animal tissues. The objective of this study was to identify and characterize potential probiotic bacteria strains from the gastrointestinal tract of free-range locally selected chickens. The bacterial isolates were screened, purified and characterized based on morphological, biochemical and molecular characteristics from 12 well-adopted free-range healthy young chickens. Low pH and bile salt tolerance, antagonistic activity, antibiotic activity, hemolysis activity, adhesion to the chicken intestine and carbohydrate fermentation tests was conducted to identify potential probiotic bacteria. Twelve bacterial isolates were screened based on their ability for their tolerance to low pH and bile salt. The isolates were identified by using 16S rRNA gene partial sequencing method. All screened isolates showed great survival percentage at low pH, that is (89.2 ± 0.75 to 97.1 ± 0.64) survived at 3 h and (83.6 ± 0.75 to 95.2 ± 0.63) at 6 h challenge at pH2. Isolate GCM112 was the least tolerant strain in 6.0% salt concentration at 12 and 24 h exposure time (82.1 ± 1.28 and 79.9 ± 1.96%) respectively. The result revealed no strain tests in this study exhibited α- and β-hemolytic activity when cultured in sheep blood agar. Most isolated strains showed best growth at 37°C temperature and up to 4% NaCl concentration. Based on the reported result from in vitro data, GCH212 and GCM412 isolates were recognized as best potential probiotic bacteria for chicken against pathogens but further studies are needed on in vivo assessment on the health benefits in the real life situation.

This study was conducted to investigate the effects of different free-range systems on the growth performance, carcass traits, and meat quality of geese. Grass pasture zones in the study area were selected, and 28 d-old male Yangzhou geese with similar body weights (1.57 ± 0.12 kg) were randomly allocated to one of three conditions: (A) free-range conditions in the apron area during 9:00 a.m.–4:00 p.m. (10–20 m from shed with grass pasture); (B) free-range conditions in the outer range from 9:00 a.m. to 4:00 p.m. (beyond 50 m from shed with grass pasture); and (C) barn system. Free range-reared geese had higher weight gain after 42 days of age than barn-reared geese, regardless of the range area. A lower feed conversion ratio was found in outer range-reared and apron area-reared geese from 28 to 63 days of age. In addition, the highest percentages of leg and breast muscle weights were observed in outer range-reared and apron area-reared geese, respectively. Finally, outer-range rearing resulted in a lower pH and lower moisture content. Therefore, these data suggest that the outer range system benefits growth performance and feed conversion ratio of geese and results in a higher percentage of leg muscle weight, lower pH, and lower moisture content. [ABSTRACT FROM AUTHOR]

Determining egg freshness is critical for ensuring food safety and security and as such, different methods have been evaluated and implemented to accurately measure and predict it. In this study, a portable near-infrared (NIR) instrument combined with chemometrics was used to monitor and predict the storage time of eggs under two storage conditions—room temperature (RT) and cold (CT) storage—from two production systems: cage and free-range. A total of 700 egg samples were analyzed, using principal component analysis (PCA) and partial least squares (PLS) regression to analyze the NIR spectra. The PCA score plot did not show any clear separation between egg samples from the two production systems; however, some egg samples were grouped according to storage conditions. The cross-validation statistics for predicting storage time were as follows: for cage and RT eggs, the coefficient of determination in cross validation (R2CV) was 0.67, with a standard error in cross-validation (SECV) of 7.64 days and residual predictive deviation (RPD) of 1.8; for CT cage eggs, R2CV of 0.84, SECV of 5.38 days and RPD of 3.2; for CT free-range eggs, R2CV of 0.83, SECV of 5.52 days and RPD of 3.2; and for RT free-range eggs, R2CV of 0.82, SECV of 5.61 days, and RPD of 3.0. This study demonstrated that NIR spectroscopy can predict storage time non-destructively in intact egg samples. Even though the results of the present study are promising, further research is still needed to further extend these results to other production systems, as well as to explore the potential of this technique to predict other egg quality parameters associated with freshness.

Simple Summary: Poultry is currently the most efficient animal productive system and could contribute to fulfilling the need for protein in order to supply the increasing human population. However, intensive selection of broilers makes them more susceptible to several welfare concerns. Moreover, in order to improve animal welfare, alternative housing systems, such as free-range and organic systems, are becoming increasingly popular. These changes in the modern poultry industry, in combination with restrictions on antibiotics, have led to the need for poultry with higher disease resistance. Naturally occurring antibodies (NAbs), which are major components of innate immunity, have been previously detected in chicken sera. These studies showed that NAbs are heritable and that high levels of NAbs are associated with survival and highly specific antibody responses against pathogens. Therefore, we investigated the circulating levels of IgM and IgY NAbs against selected antigens (actin, DNA, trinitrophenol and lipopolysaccharide) as potential biomarkers of poultry welfare and productivity in fast-growth (Ross 308) and slow-growth (Sasso) broilers raised in conventional and free-range systems, respectively, under 3-year industrial-scale production. Overall, we demonstrated significant differences in IgM NAb levels during the final breeding step between the two commercial genotypes, highlighting NAbs as potential biomarkers to be exploited in the poultry industry. Naturally occurring antibodies (NAbs), which are major components of innate immunity, exist in circulation under healthy conditions without prior antigenic stimulation and are able to recognize both self- and non-self-constituents. The present study aimed at identifying potential immunological differences between commercial fast- and slow-growth broilers (n = 555) raised in conventional and free-range systems, respectively, through the use of the specificity, isotypes and levels of circulating NAbs. The possible beneficial effect of oregano-based dietary supplementation was also evaluated. To this end, serum IgM and IgY NAbs against self- (actin and DNA) and non-self- antigens (trinitrophenol and lipopolysaccharide) were measured by ELISA and further correlated with genotype, season and performance. Significantly higher levels of IgM NAbs against all antigens were found in slow-growth compared to fast-growth broilers. IgM NAb levels were also significantly increased in dietarily supplemented slow-growth broilers versus those consuming standard feed. Moreover, significantly elevated levels of anti-DNA IgY NAbs were found in fast-growth compared to slow-growth broilers, whereas the opposite was observed for anti-LPS IgY NAbs. Multivariate linear regression analysis confirmed multiple interactions between NAb levels, genotype, season and performance. Overall, serum NAbs have proven to be valuable innovative immunotools in the poultry industry, efficiently differentiating fast-growing versus slow-growing broilers, and dietary supplementation of plant extracts can enhance natural immunity. [ABSTRACT FROM AUTHOR]

Simple Summary: Campylobacter spp. from contaminated chickens is the single largest cause of foodborne illness in the United States. Returning to traditional farming practices, such as rearing poultry on rotational pastures, is proposed to reduce human disease risks while improving animal welfare. Yet, little is understood about how environmental factors and farm management alter pathogen prevalence. Here, we assess environmental and farm management factors that impact Campylobacter spp. prevalence in poultry on 27 open-environment farms spanning the USA states of California, Oregon, Washington, and Idaho over a three-year period. We found that 26.0% of individual chicken fecal samples we tested were positive for Campylobacter spp. We detected Campylobacter spp. in ≥1 fecal sample in 69.4% of flocks and 85.2% of farms. Our analyses suggest that Campylobacter spp. prevalence increases in poultry on farms with higher average wind speeds in the seven days preceding sampling; on farms embedded in more agricultural landscapes; and in flocks typified by younger birds, more rotations, higher flock densities, and the production of broilers. Our findings indicate that farmers will face greater risk of infected poultry when there are high wind speeds, when their farms are located in highly agricultural areas, and if they raise broilers. Foodborne pathogens cause over 9 million illnesses in the United States each year, and Campylobacter from chickens is the largest contributor. Rearing poultry outdoors without the use of antibiotics is becoming an increasingly popular style of farming; however, little is understood about how environmental factors and farm management alter pathogen prevalence. Our survey of 27 farms in California, Oregon, Washington, and Idaho, USA, revealed a diversity of management practices used to rear poultry in the open environment. Here, we assess environmental and management factors that impact Campylobacter spp. prevalence in 962 individual chicken fecal samples from 62 flocks over a three-year period. We detected Campylobacter spp. in 250/962 (26.0%) of fecal samples screened, in 69.4% (43/62) of flocks, and on 85.2% (23/27) of farms. We found that Campylobacter spp. prevalence was predicted to increase in poultry on farms with higher average wind speeds in the seven days preceding sampling; on farms embedded in more agricultural landscapes; and in flocks typified by younger birds, more rotations, higher flock densities, and the production of broilers. Collectively, our results suggest that farms in areas with higher wind speeds and more surrounding agriculture face greater risk of Campylobacter spp. introduction into their flocks. [ABSTRACT FROM AUTHOR]

The cutaneous microbiome and also its resistance to antibiotics is exposed to change, depending on different habitat factors. This research investigates the composition of cutaneous microflora and its antibiotic resistance in pigs raised on free range farms (mix breed swine, from low input small farms). Swabs were collected from the skin surface and subjected to classical microbiological methods (simple broth and nutrient agar cultivation, colony isolation and biochemical API identification). The antibacterial resistance to gentamicin, streptomycin, oxitetracycline, tylosin, amoxacillin-clavulanic acid, marbofloxacin, tulatromycin, cefotaxime and doxycycline was estimated by Kirby Bauer method and multiple antibiotic resistance (MAR) index was calculated. Strains from Staphylococcus (sciuri and warnerii), Shigella spp., Kytococcus sedentarius, Salmonella spp. and Citrobacter freundii genera and species were identified in the collected samples. The most resistant was a S. warnerii strain, but the MAR index was high (0.33) in 50% of the strains. The most efficient antibiotic was cefotaxime and the least efficient was oxitetracycline. The results indicated the presence of antibiotic resistant ubiquitous and pathogenic strains in the investigated pigs which need caution, since they could express pathogenicity under appropriate conditions which low input farming system could provide. [ABSTRACT FROM AUTHOR]

ABSTRACT: Despite the existing research into the gut microbiome of meat chickens, the associations between gut microbiome composition, its activity and chicken outdoor ranging frequency remain unexplored. The aim of this study was to determine the gut microbiota composition, activity and metabolic products in chickens of 2 different lines and 3 ranging profiles. Sixty non-beak trimmed birds, either Sasso or Green-legged Partridge were housed with access to outdoor ranges from wk. 5 to 10 of age. Outdoor ranges were video recorded to obtain frequencies of the birds’ range use. The information about relative abundance of selected bacterial groups in the ceca including Lactobacillus spp., E. coli, Bifidobacterium spp., and Clostridium spp. was obtained with the PCR method. Gut microbiota activity was assessed based on the glycolytic activity of bacterial enzymes including, α-glucosidase, β-glucosidase, α-galactosidase, β-galactosidase, and β-glucuronidase as well as based on the concentration of short-chain fatty acids (SCFA) in the caecal digesta. Statistical analysis was conducted by generalized linear mixed models, applying the breed and ranging profile as fixed effects and pen as a random factor. The lowest relative abundance of Bifidobacterium spp. was found in the cecal content of indoor-preferring Sasso birds (0.01 ± 0.001), as compared to all other birds in the experiment (ranging from 0.03 ± 0.01 to 0.11 ± 0.07; P = 0.0002). The lowest relative abundance of E. coli was identified for all outdoor-preferring birds and indoor- preferring Sasso birds (0.01 ± 0.001; P = 0.0087). Cecal activity of: α-glucosidase, β-glucuronidase and β-galactosidase was higher in Green-legged Partridges, as compared to Sasso (P = 0.013; P = 0.008; P = 0.004). Valeric acid concentrations were higher in moderate Green-legged Partridges than in Sasso of the same ranging profile (2.03 ± 0.16 vs. 1.5 ± 0.17; 0.016). The majority of the current results confirmed an effect of genotype and ranging profile on the various analyzed parameters. In outdoor-preferring birds, the consumption of pasture originating feed sources as a supplement to the indoor accessible cereal-based diet likely caused the positive effects on the birds’ microbial profile.

Brazil is a big table egg producer and consumer. Although battery cages are still the main production system in the country, growth in demand for specialty eggs can be identified from the growing availability of free-range and organic eggs in the market. The goal for this study was to report the prices of different table egg categories, varying in features such as eggshell color, package size or farming system, and to characterize the information available on labels. We investigated fifteen supermarket units, randomly distributed along the west side of the city of São Paulo, state of São Paulo, Brazil. Four data collections were performed throughout one year, in each unit. Our results indicate that organic eggs were the most expensive among different farming system categories. Furthermore, brown shell eggs were more expensive compared to white shell eggs, regardless of the farming system. Eggs sold in small packages were more expensive than eggs sold in large packages. Cage-free and organic eggs had labels with more informative content and, lastly, eggs with more detailed labels were related to higher prices.

Yaks usually live in an extremely harsh natural environment resulting in low reproductive performance, so the production of yak cannot meet local demand in China. In order to solve this problem, the experiment aims to explore the effect of different feeding modes on the semen quality of male yaks, so as to provide a theoretical basis for improving the yield of yaks in Tibet. We used the combined analysis of metabolomics and microbial sequencing to explore the underlying mechanisms that affect the differences in semen quality between the house feeding (HF) system and the free range (FR). The results showed that the sperm motility (P < 0.001) and sperm concentration (P < 0.05) in the HF group were significantly higher than the FR group, and the abnormal sperm rate (P < 0.01) in HF was significantly lower compared to FR. House feeding modes increased some beneficial materials in blood and testis especially some antioxidants, unsaturated fatty acids, and amino acids. House feeding group increased some gut microbiota at genus level namely Rikenellaceae, Bacteroides, Prevotellaceae_UCG-004, Bacteroidales_RF16, and Alloprevotella, DgA-11. It was interesting that blood metabolites, testicular metabolites, and fecal microbiota were well-correlated with sperm parameters. Meanwhile, the blood metabolites and testicular metabolites were well-correlated with microbes. The result indicated that the HF model was beneficial for yak semen quality by improving the gut microbiota and blood metabolism to increase yak fertility.

In free-range and organic production systems, hens can make choices according to their needs and desires, which is in accordance with welfare definitions. Nonetheless, health and behavioral problems are also encountered in these systems. The aim of this article was to identify welfare challenges observed in these production systems in the EU and the most promising solutions to overcome these challenges. It is based on a review of published literature and research projects complemented by interviews with experts. We selected EU specific information for welfare problems, however, the selected literature regarding solutions is global. Free range use may increase the risk of infection by some bacteria, viruses and parasites. Preventive methods include avoiding contamination thanks to biosecurity measures and strengthening animals' natural defenses against these diseases which can be based on nutritional means with new diet components such as insect-derived products, probiotics and prebiotics. Phytotherapy and aromatherapy can be used as preventive and curative medicine and vaccines as alternatives to antibiotics and pesticides. Bone quality in pullets and hens prevents keel deviations and is favored by exercise in the outdoor range. Free range use also lead to higher exposure to variable weather conditions and predators, therefore shadow, fences and guard animals can be used to prevent heat stress and predation respectively. Granting a free range provides opportunities for the expression of many behaviors and yet many hens usually stay close to the house. Providing the birds with trees, shelters or attractive plants can increase range use. Small flock sizes, early experiences of enrichment and personality traits have also been found to enhance range use. Severe feather pecking can occur in free range production systems, although flocks using the outdoor area have better plumage than indoors. While many prevention strategies are facilitated in free range systems, the influence of genetics, prenatal and nutritional factors in free range hens still need to be investigated. This review provides information about practices that have been tested or still need to be explored and this information can be used by stakeholders and researchers to help them evaluate the applicability of these solutions for welfare improvement.

Context. Few studies have been conducted with the Brazilian Embrapa 051 hens to improve the feed conversion rate and control skeletal health in this breed. Aims. To determine how different feeding volumes affect the laying rate and bone quality of the Embrapa 051 (E051) strain in comparison with Lohmann Brown (LB). Methods. In total, 600 E051 and 200 LB hens were subjected to the following treatments: (1) control (LB fed 100% of their dietary requirements), (2) E051 fed 93% of the control diet, (3) E051 fed 100% of the control diet, and (4) E051 fed 107% of the control diet. Key results. Throughout the 37-72 weeks of age, LB hens presented a higher egg production rate than E051 hens (P < 0.05). The bodyweight and egg production rate were lower in E051 when hens received 93% of the E051 control diet. The E051 hens achieved higher values for tibia weight and length and Seedor index than did LB hens (P < 0.05). At Week 40, the E051 hens fed 93% of the control diet presented a greater tibia weight (12.6 vs 11.5 g), length (124.5 vs 118.9 mm) and strength (21.9 vs 15.5 kgf), Seedor index (101.7 vs 96.13), and ash (33.0 vs 29.6%), calcium (11.1 vs 9.8%) and phosphorus (4.9 vs 4.5%) concentrations than did LB hens. However, at 73 weeks of age, the only differences observed in favour of E051 hens fed 93% of the control diet were for tibia weight, tibia length and tibia strength in comparison with LB hens (P < 0.05). Conclusions. LB hens showed a higher performance, but lower bone quality than did E051 hens. E051 receiving 7% less feed showed a reduced laying rate but, in contrast, better bone quality. This study showed that the best feeding strategy for Embrapa 051 hens was to use the same feeding volume as recommended for Lohmann Brown hens. Implications. Despite E051 hens showing a lower laying rate than that of LB hens, especially when receiving a restricted amount of feed, E051 is an accessible alternative breed with excellent bone quality for free-range systems in Brazil. [ABSTRACT FROM AUTHOR]

Despite consumers' belief that access to an outdoor range improves chicken welfare, still little is known about whether this is true and whether individual ranging profiles relate to the birds' welfare. The aim of the present study was to identify and compare welfare issues of the traditional broiler hybrid Sasso and the Polish heritage chicken Green-legged Partridge, having outdoor access, and examine if the birds' welfare status was associated with the ranging profile: outdoor-preferring, moderate-outdoor, and indoor-preferring. In August 2018, 60 non-beak trimmed birds per genetic strain were housed in groups of 10 from weeks 5 to 10, under conditions exceeding EU requirements of organic meat chicken production. Each pen had access to an individual outdoor range that was video-recorded continuously to obtain frequencies of individual birds' use of the ranges. Plumage condition, comb pecking wounds, skin injuries, dirtiness, toe damage, eye pathologies, footpad dermatitis, hock burns, respiratory infections, diarrhea, and walking difficulties were assessed at the end of the experiment. Statistical analysis was conducted applying generalized linear mixed models, with binomial distribution and logit link using SAS software, applying breed and ranging profile as fixed factors and their interaction, with pen as random factor. A tendency for more respiratory infections was observed in Sasso birds from each ranging profile, as compared to matching ranging profiles presented by Green-legged Partridges (outdoor-preferring: P = 0.0012; moderate-outdoor: P < 0.0001; and indoor-preferring: P = 0.0247). Indoor-preferring Green-legged Partridges tended to present more respiratory infections, as compared to the 2 other ranging profiles within the breed (outdoor-preferring: P = 0.0291; moderate-outdoor: P = 0.0448). Regardless of the breed, toe damages were more frequent in indoor-preferring birds, as compared to other ranging profiles (P = 0.017). It remains unknown whether the use of outdoor areas prevents development of welfare issues or if birds with a suboptimal welfare condition become indoor-preferring individuals.

Two spent hen breeds (Mos and Isa Brown) fed with three different diets [commercial feeding (CF); corn, pea, and triticale (CPT); and corn and wheat (CW)] were studied to assess their laying performance, carcass characteristics, and meat quality parameters (breast and drumstick). A total of 48 hens reared in a traditional free-range system were used. Birds were slaughtered at 72 weeks of age after the moulting in an accredited abattoir, and measurements (for each bird) of carcass and meat started at 24 h post-mortem. The results demonstrated a breed and diet effect (P<0.05) on laying performance, carcass, and meat quality. The Mos breed showed lower laying performance, higher live weight (P<0.001), carcass weight (P<0.001), dressing percentage (P<0.001), and carcass fat (P<0.001) than Isa Brown. The breed also affected physicochemical parameters of drumstick and breast (P<0.05). Concerning the diet effect, spent hens fed with commercial feeding showed heavier live weight (P<0.01) and carcass weight (P<0.01). Diet significantly affected most of meat quality parameters, CPT being the formulation that most affected (P<0.05) drumstick composition, while in the breast was CF (P<0.001). Significant (P≤0.005) effects on breast colour, cooking loss and hardness were observed in animals fed with alternative diets to CF. Regarding nutritional quality, no breed or diet effect (P<0.05) was observed in MUFA s, PUFA s and n-6 contents of meat. Overall, both breeds showed a high protein and low-fat content in the breast, showing these hens as an opportunity for the development of meat products as well as an economic purpose for spent hens that reach the end of their productive lives. [ABSTRACT FROM AUTHOR]

The study was carried out to demonstrate the effects of the use of water-extracted propolis (PE) as a coating material on shelf life, storage temperature, time, and egg quality parameters obtained from free-range laying hens. The idea of coating an egg with a product to preserve its freshness for a longer time has been the subject of research by researchers. For this purpose, there are studies using samples of propolis extracted using different alcohols. In this study, the effects of coating eggs obtained from free-range reared chickens with water extracted PE and keeping them in different storage conditions on the internal and external quality parameters of the eggs were investigated. For this purpose, 80 eggs were collected from 64-week-old Lohmann brown layers. Egg samples were divided into groups of 8 treatments [2 storage times (10 and 20 days) × 2 storage temperatures (4°C and 22°C) × propolis coating on the shell (coated / uncoated)] and arranged to contain 10 eggs per treatment. In the study, it was seen that PE caused positive effects on the shelf life and internal quality characteristics of eggs stored at room and refrigerator temperature, as it covers the pores on the shell. As a result of coating with propolis extract, specific gravity and egg yolk index values increased (P <0.05). In addition, the number of mesophilic bacteria has decreased. Due to these positive effects, propolis can be considered as a natural alternative product that can be used for eggshell coating. It was determined at the end of the research that coating with the water-derived extract of propolis, a by-product of beekeeping activities, might have positive effects on long-term storage. [ABSTRACT FROM AUTHOR]

ABSTRACT: Mandating free range husbandry as a requirement for organic egg designation remains a prevailing sentiment within a segment of the organic community. The proponents maintain that such management practice ensures high hen welfare and enhanced wholesomeness of the egg. However, evidence from the field, especially in the European Union (EU), contradicts these assumptions. In many cases, hens allowed outdoor access were more subject to increased injury from predators and from flock mates, disease was more prevalent and generally more severe, and, as a result, higher mortality was routinely observed in these individuals compared with those raised indoors. The safety of eggs from free range hens is also questionable. Outdoor access compromises biosecurity efforts to curtail interaction of hens with rodents and wild birds, increasing the risk of flock Salmonella enterica serovar Enteritidis infection and consequent production of Salmonella-contaminated eggs. Even more serious, soil contaminated with dioxins and polychlorinated biphenyls, carcinogenic industrial by-products widespread in the environment, can be ingested by hens foraging outdoors. These compounds will subsequently be deposited into the egg yolks, many times at high levels, creating a serious food safety issue for the consuming public. Such findings provide evidence that hens exposed to a free-range environment may exhibit neither an enhanced welfare nor produce the safe wholesome egg that consumers expect.

Introduction: Smaller scale, alternative, chicken production systems are gaining popularity globally. However, this brings public health and market confidence concerns, especially where there are no established standards of production. The aim of this study was to carry out a microbiological analysis of chicken carcasses from the commercial, backyard and semi-backyard production systems, slaughtered in the same slaughterhouse. Methodology: Samples of 102 chicken carcasses were taken in two steps of the slaughter (A: after bleeding; and B: after chiller tank) and were subjected to aerobic mesophilic, coliforms at 35 °C and coliforms at 45 °C counts, and Salmonella spp. detection. Salmonella spp. isolates were subjected to antimicrobial resistance analysis. Results: At slaughter step A, carcasses from the backyard system had less contamination than carcasses from the commercial system, with a difference of 0.7 log10 CFU/mL. Salmonella was identified in carcasses of all production systems and in both slaughter steps. Nine chicken carcasses were positive for Salmonella and no significant difference was observed in the occurrence of Salmonella amongst the carcasses from different production systems. Two Salmonella isolates, that presented the highest resistance profiles (one isolate was resistant to eight and the other to six out of ten tested antibiotics), were identified on carcasses from the semi-backyard system. Conclusions: Carcasses from the backyard system had a lower microbial count at the initial step of the slaughter process than the commercial production system. In addition, greater resistance to antimicrobials was observed in Salmonella isolates from semi-backyard system. [ABSTRACT FROM AUTHOR]

Sheep and goats raised extensively are frequently infested by Ixodid ticks that may act as vectors or reservoirs of Spotted Fever Group Rickettsiae (SFGR). A study to determine the seroprevalence of SFGR infection in 300 sheep and goats in Plateau State, Nigeria was conducted from September to November, 2018 using the Indirect Fluorescence Antibody Test (IFAT). Overall, 85 out of 300 animals (28.3%) were seropositive to SFGR. Relatively higher seroprevalence was recorded in sheep than goats (28.8% vs 28.0%) but the difference was not statistically significant (p > 0.05). Furthermore, seropositivity was not affected by age, sex or location of the animals screened in this study. This is the first serological study to report the prevalence of SFGR in sheep and goats using IFAT in this study area. The presence of SFGR antibodies in domestic ruminants is of public health concern considering the close association between farmers and their animals occasioned by the management system practiced in the study area. This finding calls for further studies to evaluate the level of human exposure to this group of pathogen. [ABSTRACT FROM AUTHOR]

1. The objective of this study was to evaluate specific sensory attributes of chicken meat from different production systems to evaluate the emotional responses related to information available on the packages, as well as to investigate consumers' perceptions regarding this information. 2. The survey was conducted using the Temporal Dominance of Sensations analysis, which identified differences in taste and texture between samples belonging to the organic, conventional and natural systems. 3. The specific emotional lexicon for chicken meat, defined by the consumers through the EmoSemio questionnaire, provided a list of positive emotions for packages that indicated some type of information about the rearing or feeding system. 4. The qualitative data obtained using the Attitude Questionnaire demonstrated that packaging and labelling are not self-explanatory, posing more doubts for the consumer at the time of purchase. It was shown that packaging and labelling were extrinsic attributes that influenced the evaluation and acceptability of products. Besides that, consumers were interested in buying products that attest to animal welfare, provided that the price is attractive. 5. Poultry companies can have a greater chance of making their products more attractive if they learn more about the attitude and expectations of the consumer in relation to the products, as well as the importance of the animal welfare attributes that are communicated. [ABSTRACT FROM AUTHOR]

The rearing of pigs in the mountains of Papua is generally based on the free-range method during the daylight. Poor environmental sanitation and free-range management have been reported as risk factors of gastrointestinal worm infestation in pigs. In general, gastrointestinal parasite infestation can cause a loss of appetite, impede growth, decrease feed conversion rate, and increase vulnerabilities toward other pathogens. To add to that, the acute levels of infestation can cause death. Furthermore, some species can infest humans (zoonotic). As zoonotic parasites can impact the productivity and health of the people, it is crucial to identify the species of such parasites. This research was conducted in Umpakalo village at Kurulu district, Jayawijaya region, and three other villages namely Obaipugaida, Eyagitaida, and Ugituwataida at Ekadide district, Paniai region. Forty-five fecal samples from different pigs were used as the research material. The native method was used to identify the physical appearance of the eggs, and in turn, identify the species of the parasite. The data was analyzed in the tabulation form. The conclusion obtained in this research is that 95% of the fecal samples were infected by the gastrointestinal worms. Identified worms are Ascaris suum, Strongyloides ransomii, Trichuris suis, and Strongyle types. Most pigs have mixed infections.

Pasture access for dairy cows is highly valued both by cows and the public at large. When pasture access is not feasible, farmers can provide cows with alternative forms of outdoor access, such as an outdoor bedded pack, that may be easier to implement on some farms. We reviewed the literature on how lying, standing, walking, feeding, social, and estrus behaviors are influenced by pasture and other types of outdoor areas. Pasture allows the expression of grazing and can facilitate the expression of lying, standing, walking, and estrus behaviors. In addition, pasture can decrease the number of negative social interactions between cows, likely because more space per cow is provided than what is normally available indoors. The provision of soft flooring and an open space in outdoor bedded packs appears to provide some benefits for lying, standing, and walking behavior and may also have positive effects on social behavior, especially with larger space allowances. The effects of an outdoor bedded pack on estrus behavior are less well-documented, but the provision of a standing surface that provides better footing than typically available indoors may promote estrus behavior. Alternative outdoor areas assessed to date appear to be less attractive for cows than pasture, perhaps because these areas do not provide the opportunity to graze. We encourage future research to investigate the importance of grazing for dairy cows. The motivation of dairy cows to access alternative outdoor areas should also be investigated. As cow preference for the outdoors depends on many factors, providing cows a choice may be of particular importance.

Currently, the poultry industry has been faced with consumer pressure to utilize only vegetable feedstuffs in poultry diets, eliminate antibiotics from poultry production, and rear poultry in free range systems. To maintain current production standards, the industry must determine ways to enhance nutrient uptake and utilization further. One possible solution is the supplementation of pectinase, an enzyme that degrades pectin within the cell walls of plants, in poultry diets. Therefore, the objective of the current study was to determine the potential role of a pectinase producer, Dickeya dadantii DSM 18020, as a commercially utilized pectinase producer in poultry diets against other known pectinase producers, in silico. In the current study, whole genomes of Dickeya dadantii DSM 18020 (Dd18020), D. dadantii 3937 (Dd3937), D. solani IPO 2222 (Ds2222), Bacillus halodurans C-125 (BhC125), and B. subtilis subsp. subtilis str. 168 (Bs168) were compared using bioinformatic approaches to compare the chromosomal genome size, GC content, protein coding genes (CDS), total genes, average protein length (a.a.) and determine the predicted metabolic pathways, predicted pectin degrading enzymes, and pectin-degradation pathways across pectinase producers. Due to insufficient information surrounding the genome of Dd18020 (lack of annotation), the genome of Dd3937, a 99% identical genome to Dd18020, was utilized to compare pectinase-associated enzymes and pathways. The results from the current study demonstrated that Dd3937 possessed the most significant proportion of pathways presented and the highest number of pathways related to degradation, assimilation, and utilization of pectin. Also, Dd18020 exhibited a high number of pectinase-related enzymes. Both Dd3937 and Dd2222 shared the pectin degradation I pathway via the EC 3.1.1.11, EC 3.2.1.82, and EC 4.2.2.- enzymes, but did not share this pathway with either Bacillus species. In conclusion, Dd18020 demonstrated the genetic potential to produce multiple pectinase enzymes that could be beneficial to the degradation of pectin in poultry diets. However, for Dd18020 to become a commercially viable enzyme producer for the poultry industry, further research quantifying the pectinase production in vitro and determining the stability of the produced pectinases during feed manufacturing are necessary.

1. Ascarids (Ascaridia galli and Heterakis spp.) are highly prevalent in free-range laying hens. Ascarid eggs survive for long periods in soil, and one preventive measure is to add litter material to areas close to the henhouse. In this study, recovery rates of ascarid eggs from three common litter materials, namely pea gravel, beech (Fagus sylvatica) and spruce (Picea abies) wood chips were compared to recovery rates from soil. 2. Materials were mixed with faeces containing 1,408 ascarid eggs per g of faeces, placed in plastic fruit boxes and exposed to natural weather conditions in a randomised block design with six replicates per treatment. 3. Numbers of ascarid eggs were quantified at 28 time points over 3.5 years. Ascarid eggs were recovered for over three years from all materials and completely disappeared during the fourth winter of exposure. Time needed to get to a 50% reduction in ascarid eggs did not differ between litter materials and soil (242 to 269 days). A 99% reduction was reached significantly (P < 0.001) earlier in pea gravel (548 days) than in the three other materials, and earlier in the two wood chips (day 682 for beech, day 692 for spruce, P < 0.05) than in soil (1,277 days). 4. Accumulation of ascarid eggs in the area close to the henhouse can be reduced by any of the tested litter materials compared to bare soil. Adding litter to this area is highly recommended for free-range layer farmers in order to reduce numbers of infective ascarid eggs. [ABSTRACT FROM AUTHOR]

The ability to use heterospecific alarm calls is adaptive in the wild, as it provides an opportunity to avoid predators. We now know that several species are able to respond to alarm calls intended for others. However, this capacity has never been investigated in domestic animals. The capacity to use heterospecific alarm calls may be relevant for free-range domestic species, especially when they share predators with wild signallers. Using playback experiments, we investigated the vigilance behaviour of free-range naked neck chickens (Gallus gallus domesticus) when confronted with alarm calls (test playbacks) and songs (control playbacks) of a commonly occurring wild passerine, the great tit (Parus major). We found that subjects exhibited an increased vigilance to alarm calls compared to songs, therefore, showing that chickens respond to heterospecific signals as wild birds do. Recently, there has been an increased interest for free-range poultry production, notably because of the benefits of this farming method for chicken welfare. Although future studies are required to address this question, mortality due to predation may be reduced through the implementation of structures in areas frequented by wild heterospecific signallers. [ABSTRACT FROM AUTHOR]

The mainstreaming of ethical consumption over the past two decades has attuned citizen-consumers to their power to shape food production practices through their consumption choices. To navigate the complexity inherent in contemporary food supply chains, ethical consumers often turn to certification and labelling schemes to identify which products to purchase. However, the existence of competing supply chain interests, coupled with the myriad different ways production factors and processes can be combined, has constructed certification and labelling as a highly contested space. Within this context, celebrity chefs have taken on a significant role in influencing food cultures, consumption practices and public policy. As a group of powerful cultural and political intermediaries, celebrity chefs have used their public profile to address causes related to food ethics and sustainability, and to shape consumer 'choice' by advocating for the consumption of labelled and certified food products. This article analyses the media campaigns of British celebrity chefs Jamie Oliver and Hugh Fearnley-Whittingstall to promote 'free range' chicken and eggs. It reveals how the celebrity chefs' interventions into consumption politics often occurs without sufficient sensitivity to the specificities of the particular labelling and certification systems they are promoting, with very different systems often presented as achieving identical ends. In presenting 'free range' as a single, idealised and uncontested standard, they (perhaps unwittingly) expose themselves to the range of contradictions involved in the need to present complex information on animal friendly and sustainably produced food in simple, unambiguous and entertaining formats. [ABSTRACT FROM AUTHOR]

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The present study evaluated performance, physiological response and economics of commercial fast growing (CFG), commercial slow growing (CSG), and Rhode Island Red (RIR) chickens under intensive and free-range rearing environments. After 21 days of rearing under the same intensive environment 240 birds from each strain were subjected to free-range and intensive rearing until they were 56 days old. Each treatment was replicated six times with 20 birds in each replicate. Body surface and cloacal temperatures, respiration and heart rates, feed intake, bodyweight and weight gain, feed conversion efficiency, growth efficiency, and liveability were recorded. Significant differences among strains were detected in physiological response and growth performance (except liveability). Rearing environment also caused significant differences in physiological parameters (except body surface temperature) and growth performance (except liveability). Significant interactions of the strains and production systems were detected. The CFG strain grew most rapidly under the intensive system with differences between strains being reduced in the free-range system. The RSG and CSG strains had similar respiration rates under the two production systems but differed significantly from each other. However, the CFG strain had a significantly elevated respiration rate in the free-range system. Total input cost of rearing CFG under the intensive system was highest ($3.54) among the treatments, whereas CSG under a free-range environment generated the highest profit ($0.37 per bird). In conclusion, rearing CSG under free range was the most economic farming strategy in today's scenario. [ABSTRACT FROM AUTHOR]

Providing dairy cows access to pasture is desirable, given their strong motivation for pasture access, but this practice is hindered by several practical constraints, including lack of available pasture. An alternative to pasture is an outdoor area that is bedded with a soft material such as sand or wood chips that requires less space than pasture, given the absence of soil or grass that can otherwise be damaged by cow traffic. However, little is known about space requirements for alternative outdoor areas. This study investigated how space allowance affected cow preference to be outdoors as well as cow behavior on an outdoor pack. A total of 3 groups of 24 pregnant, lactating, healthy Holstein cows were used. Each group was given 3 d for social dynamics to stabilize; during this time cows were kept indoors in a freestall pen. A habituation phase of 5 d followed, where animals were given free access to the outdoor pack with a space allowance of 16 m2/cow. Cows were moved outside (if not already outdoors) at set times each day during the habituation phase (i.e., 5 times during the first 2 d and 2 times during the last 3 d). Cows were then given free access to the outdoor pack, but space allowance was changed every day. A total of 13 different space allowances were randomly applied, without replacement, ranging from 4 to 16 m2/cow in 1-m2 increments. Using continuous video recordings, the location of the cows (i.e., in the freestall barn or on the outdoor pack) as well as displacements from a lying position on the outdoor pack were scored. Standing and lying behaviors were automatically measured using HOBO data loggers (Onset, Cape Cod, MA). Over the 24-h period, cows spent more time outside with increasing space allowance, but this result was driven almost entirely by the increased time spent outdoors during the nighttime hours. During the night, space allowance did not influence the number of displacements from lying on the outdoor pack or the proportion of time on the outdoor pack that cows spent lying down. Our results indicate that cows use an outdoor bedded pack mostly at night and that the time spent outside at night increases with increasing space allowance. Providing an outdoor bedded pack should be considered when designing dairy cattle housing systems. [ABSTRACT FROM AUTHOR]

Hens of the laying hybrid ISA Brown were used in the study with the objective to evaluate eggshell quality, microbial contamination of eggshells, and penetration of microorganisms into the egg content in different housing systems (enriched cage: 60 hens, 10 hens per cage, 750 cm2 per hen vs free range: 60 hens, 9 hens per m2) and at different hen ages (26 vs 51 weeks) during storage time (0, 2, 7, 14, and 21 days). A significant interaction between the housing system and age was observed in egg weight and most of eggshell quality measurements. However, microbial contamination and penetration were affected mostly by the housing system and storage time. The numbers of Escherichia coli (P < 0.001, 4.51 vs 2.75 log cfu/eggshell) and Enterococcus (P < 0.001, 2.56 vs 1.11 log cfu/eggshell), and the total number of microorganisms (P < 0.001, 5.04 vs. 3.65 log cfu/eggshell) were higher in free range eggs compared to enriched cage eggs, respectively. The counts of Escherichia coli (P < 0.001, 4.23 vs 2.91 log cfu/eggshell) and Enterococcus (P < 0.001, 2.31 vs 1.27 log cfu/eggshell) decreased with storage time. A positive correlation between the total number of pores and penetration of Escherichia coli in both housing systems was observed in the albumen. It can be concluded that the housing system and age of laying hens significantly affected eggshell quality. Microbial contamination presumably affects the penetration of microorganisms. The correlation between the number of pores and penetration is assumed to be affected by the microbial species.

Free-range systems are considered to improve bird health and welfare, thereby satisfying consumer demands. Behavioral time budget, fear level and clinical welfare indicators were compared for two Turkish laying hen genotypes, Atak-S (brown) and Atabey (white), reared in a free-range system. A total of 420 laying hens (210 Atak-S, 210 Atabey) were studied between 19 and 72 weeks of age. Higher percentages of eating and drinking behavior, feather pecking, and explorative pecking were observed for Atak-S hens, whereas Atabey hens were preening, walking–standing, and resting more. The duration of tonic immobility was longer, and the number of inductions was lower in Atabey compared with Atak-S hens. Atabey hens had less keel bone damages and better plumage conditions on the breast, wing, and tail at 56 and 72 weeks of age than Atak-S hens. Footpad dermatitis was more common in Atabey hens at 40 weeks, whereas Atak-S hens had a higher prevalence of footpad dermatitis with moderate lesions at 72 weeks of age. These findings indicate that free-range Atak-S hens may be more prone to keel bone damage and development of feather pecking, but they showed less foot lesions and were less fearful.

The purpose of this experiment was to investigate the behaviour of fast- and slow growing chickens, when the birds had access to the outdoors. Fifty of both 1-day-old male chicks of a laying hybrid ISA BROWN (IB) and broilers ROSS 308 (RS) were kept in two pens in the same building. In each group ten birds were marked with a colored spray. From three weeks of age the birds had access to the outdoors. From the age of one to seven weeks old, one day a week the marked birds were observed from 8:00 to 18:00 every ten minutes and the activity was noted. At 49 and 90 days old twenty birds were slaughtered and the strength of bones was measured. The behaviour was expressed as percentage of the time spent in activity. The average time that the birds spent eating during the 7 week period was almost the same in IB and RS. On average, IB birds spent significantly higher (P

A total of 211 pheasants (Phasianus colchicus) were reared using two different production systems (free range and intensive) to determine the effect of production system and slaughter age on growth performance and carcass traits. Birds were slaughtered at 14, 16, and 18 weeks of age. Production system did not significantly affect body weight until 18 weeks; at this age, pheasants reared in free range exhibited a higher average weight (1149 g vs. 1012 g). Total feed consumption and feed conversion ratios did not differ among production systems. The most in-demand portions of the carcass (breast and leg) were not affected by differences in production system. The leg and breast proportions which were the most edible parts of the carcass were around 26% and 35%, respectively, in both free-range and intensive systems. [ABSTRACT FROM AUTHOR]

The objective of the study was to evaluate changes during storage time (0, 7, 14, and 21 D) in the weight, albumen quality and its functional properties, and protein content of eggs from enriched cage and free range at 26 and 51 wk of age. Egg weight was affected by a significant interaction between the housing system and the age of hens. Regarding individual factors, heavier eggs (P < 0.001) were obtained with the enriched cage (61.7 g) environment than with free range (59.6 g) environment at 51 wk (P < 0.001) (63.3 g vs. 58.0 g), and freshly laid eggs were heavier compared to eggs stored for 14 and 21 D (P < 0.001). The significant interaction of evaluated factors affecting egg weight loss included the Haugh unit score and pH. Albumen dry matter content was significantly higher in free-range eggs (14.70% vs. 14.0%), in eggs from younger hens (15.0% vs. 13.7%) and in eggs that were stored 21 D. Free-range eggs contained more lysozyme (P < 0.001), and younger hens produced eggs with higher ovotransferrin (P < 0.05) and ovalbumin content (P < 0.01). The data from the study show that the evaluated factors and their interactions significantly affected the physical parameters of the eggs, whereas albumen functional properties and lysozymes, ovalbumin, and ovotransferrin were negligibly affected. [ABSTRACT FROM AUTHOR]

Highly adapted plant species suited to low rainfall conditions need to be considered for free-range poultry farms, particularly in marginal rainfall areas. A group of suitable plants are the saltbushes, in particular old man saltbush (Atriplex nummularia) can provide shelter, shade, and possibly forage. The first experiment examined whether hens eat old man saltbush while ranging. Free-range Hy-Line Brown layer hens were provided saltbush or "no" saltbush on the outdoor range throughout 11 wk of early production (16 to 27 wk). The quantity of saltbush eaten by the hens was determined by the n -alkane method. Hen interactions with saltbush were video recorded, and hen live weight, feed intake, egg production, and egg quality were measured. Hens ate the saltbush at 5% of their dietary dry matter intake. This level of saltbush intake had no influence on egg production. The video footage revealed hens actively pecked at the saltbush. The second experiment investigated the consequences of hens diluting their diet by eating increased levels of River saltbush (Atriplex amnicola). Air-dried, hammer-milled river saltbush was mixed and pelleted into a standard commercial layer diet at the following levels; 0 (control), 5, 10, 15, and 20%. Hy-Line Brown layer hens were fed the diets for 28 D (32 to 35 wk of age). Seventy-five hens were housed in individual cages, with 15 hen replicates per diet. The saltbush had no significant impact on egg production, hen live weight, or feed intake. Excreta moisture increased significantly (P < 0.001) with increased saltbush (5, 10, 15, and 20%) in the diet. Furthermore, high saltbush eggs (20%) had significantly stronger egg yolk color (P = 0.006) and thicker egg shells (P < 0.001) compared to the control eggs. Consumers were presented with the eggs from the second experiment and they preferred the high saltbush eggs (20%). In conclusion, saltbush would be a valuable plant on free-range poultry farms to provide shade and shelter, especially in those areas with marginal rainfall. [ABSTRACT FROM AUTHOR]

This study investigated the laying performance, egg quality, and egg yolk fatty acids (FAs) and cholesterol content in layer hens housed with free access to chicory- and/or white clover-vegetated areas. During a 16-week study, 400 Lohmann Brown hens (32 weeks old) housed with free outdoor access were allocated randomly into four groups, each with four replicates of 25 hens. Control hens were fed a conventional diet with free access to a soil area (C), whereas other hens were fed on a conventional diet with free access to a chicory (CI)- or white clover (TR)-vegetated area or a CI and TR mixture (MIX)-vegetated area. The C hens consumed more concentrate feed (p = 0.018) than the TR and MIX hens, which had a higher herbage intake than the CI birds (p < 0.001). The C hens produced eggs with a thicker shell than those in the other treatment groups (p = 0.013). Compared with C, the saturated FAs of egg yolk decreased for MIX (p = 0.010). The polyunsaturated FAs were higher in the MIX eggs than in the C and TR eggs (p < 0.001). Although FAs were distributed in all quadrants of the principal component analysis (PCA), three main FA profiles could be identified based on the loadings of natural groupings in the PC2 versus PC1 plot. The present study shows clear evidence for the contribution of herbage to the hen diet without affecting laying performance. In addition, the FA composition of the CI and MIX vegetation contributed to the production of eggs with preferred FA attributes, such as polyunsaturated FAs and a favourable n-6 to n-3 ratio.

Consumer interest in buying eggs from animal welfare-friendly systems with outdoor access is increasing, leading to an increase in the need for knowledge on genotypes suitable for free-range systems. Two Turkish laying hen genotypes, Atak-S (brown, n = 210) and Atabey (white, n = 210), were reared in a free-range system from 19–72 weeks of age, and their suitability for the system was assessed based on laying performance, egg quality, and yolk fatty acid profile. Mean hen-day and hen-housed egg production were found to be higher in Atabey than Atak-S (p < 0.01). The brown eggs from Atak-S hens tended to be heavier than the white eggs from Atabey hens (p < 0.01). Brown eggs obtained from Atak-S hens had a stronger shell structure (p < 0.01), while white eggs from Atabey hens had higher mean yolk index, albumen index, and Haugh unit than brown eggs (p < 0.05). At 56 weeks of age, total saturated fatty acid content in yolk was higher in white eggs than in brown eggs (p < 0.01). These findings related to genotype could help free-range egg producers in their choices for more profitable production and for meeting consumer demands on egg quality and egg yolk fatty acid levels.

Free-range use by broiler chickens is often limited, whereas better use of the free-range area could benefit animal welfare. Use of free-range areas could be stimulated by more appropriate shelter or environmental enrichment (by decreasing birds’ fearfulness). This study aimed to assess the effects of shelter type, early environmental enrichment and weather conditions on free-range use. Three production rounds with 440 slow-growing broiler chickens (Sasso T451) were carried out. Birds were housed indoors in four groups (two with males, two with females) from days 0 to 25, during which two of the groups received environmental enrichment. At day 23 birds’ fearfulness was assessed with a tonic immobility (TI) test (n=100). At day 25 all birds were moved (in mixed-sex groups) to mobile houses, and provided with free-range access from day 28 onwards. Each group could access a range consisting for 50% of grassland with 21 artificial shelters (ASs, wooden A-frames) and for 50% of short rotation coppice (SRC) with willow (dense vegetation). Free-range use was recorded by live observations at 0900, 1300 and 1700 h for 15 to 21 days between days 28 and 63. For each bird observed outside the shelter type (AS or SRC), distance from the house (0 to 2, 2 to 5, >5 m) and its behaviour (only rounds 2 and 3) were recorded. Weather conditions were recorded by four weather stations. On average, 27.1% of the birds were observed outside at any given moment of observation. Early environmental enrichment did not decrease fearfulness as measured by the TI test. It only had a minor effect on the percentage of birds outside (0.4% more birds outside). At all distances from the house, SRC was preferred over AS. In AS, areas closer to the house were preferred over farther ones, in SRC this was less pronounced. Free-range use increased with age and temperature and decreased with wind speed. In AS, rainfall and decreasing solar radiation were related to finding more birds outside, whereas the opposite was true in SRC. Behaviour of the birds depended on shelter type, distance from the house, early environmental enrichment, time of day and age. Chickens ranged more and farther in SRC, possibly because this provided a greater sense of safety because of the amount of cover and/or better protection against adverse weather conditions. These results indicate that SRC with willow is a more appropriate shelter for slow-growing broiler chickens than A-frames.