1. The gas 5 gene shows four alternative splicing patterns without coding for a protein
- Author
-
Daniela Rossi, Virginia Barone, G. Raho, Louis H. Philipson, and Vincenzo Sorrentino
- Subjects
Cell Extracts ,DNA, Complementary ,Blotting, Western ,Molecular Sequence Data ,genetics/metabolism ,Sequence Homology ,Biology ,chemistry ,Exon ,Mice ,Open Reading Frames ,Putative gene ,Complementary ,Sequence Homology, Nucleic Acid ,Animals ,RNA, Small Nucleolar ,genetics ,Small nucleolar RNA ,Gene ,Small Nucleolar ,Genetics ,Nucleic Acid ,Base Sequence ,Blotting ,Alternative splicing ,Intron ,General Medicine ,DNA ,3T3 Cells ,Molecular biology ,Stop codon ,Rats ,Open reading frame ,Alternative Splicing ,RNA ,3T3 Cells, Alternative Splicing ,genetics, Animals, Base Sequence, Blotting ,Western, Cell Extracts ,chemistry, DNA ,genetics, Mice, Molecular Sequence Data, Open Reading Frames, RNA ,genetics/metabolism, Rats, Sequence Alignment, Sequence Homology ,Western ,Sequence Alignment - Abstract
The murine gas5 gene was originally isolated based on its preferential expression in the growth arrest phase of the cell cycle. This gene contains 12 exons from which two alternatively spliced transcripts have been initially identified. More recently, it has been reported that both human and murine gas5 genes contain in their introns sequences homologous to the small nucleolar RNAs involved in the processing of ribosomal RNA. Here we report on the identification and analysis of the expression pattern of two novel alternatively spliced mouse gas5 mRNAs which contain no obvious open reading frame (ORF). Using antibodies generated against the putative amino acid sequence deduced from the gas5 cDNAs, we were not able to detect any Gas5 protein in cultured cells or murine tissues extracts. Even more definitive evidence that the gas5 gene may not encode a protein was obtained by cloning and sequencing the rat gas5 gene which revealed that the putative ORF is interrupted by a stop codon after the first 13 amino acids.
- Published
- 2000